##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934432.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1941052
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17023346103041	33.0	31.0	34.0	30.0	34.0
2	32.361453995050105	34.0	31.0	34.0	30.0	34.0
3	32.42539251910819	34.0	31.0	34.0	30.0	34.0
4	35.93306310186435	37.0	35.0	37.0	35.0	37.0
5	35.88279603019394	37.0	35.0	37.0	35.0	37.0
6	35.51203728699695	37.0	35.0	37.0	33.0	37.0
7	35.882531740520086	37.0	35.0	37.0	35.0	37.0
8	35.96925481646035	37.0	35.0	37.0	35.0	37.0
9	37.763462802645165	39.0	38.0	39.0	35.0	39.0
10	37.236605201715356	39.0	37.0	39.0	34.0	39.0
11	37.09141692236993	39.0	37.0	39.0	33.0	39.0
12	36.7904208645621	39.0	35.0	39.0	33.0	39.0
13	36.637604762778125	39.0	35.0	39.0	33.0	39.0
14	37.43336551519485	40.0	36.0	41.0	32.0	41.0
15	37.57913698345021	40.0	36.0	41.0	32.0	41.0
16	37.74510471641151	40.0	36.0	41.0	33.0	41.0
17	37.7367412104364	40.0	36.0	41.0	33.0	41.0
18	37.72767138644405	40.0	36.0	41.0	33.0	41.0
19	37.768153042782984	40.0	36.0	41.0	33.0	41.0
20	37.6711113355026	40.0	36.0	41.0	33.0	41.0
21	37.526509336174406	39.0	35.0	41.0	33.0	41.0
22	37.673785143314035	39.0	35.0	41.0	33.0	41.0
23	37.65704628211918	39.0	35.0	41.0	33.0	41.0
24	37.60297354218228	39.0	35.0	41.0	33.0	41.0
25	37.53353439269015	39.0	35.0	41.0	33.0	41.0
26	37.4658133836703	39.0	35.0	41.0	33.0	41.0
27	37.380874907009186	39.0	35.0	41.0	33.0	41.0
28	37.27388962274066	39.0	35.0	41.0	32.0	41.0
29	37.203345917574595	39.0	35.0	41.0	32.0	41.0
30	37.14599042168886	39.0	35.0	41.0	32.0	41.0
31	37.05286772327583	39.0	35.0	41.0	31.0	41.0
32	36.97495687905322	39.0	35.0	41.0	31.0	41.0
33	36.932461881495186	39.0	35.0	41.0	31.0	41.0
34	36.76161174455913	39.0	35.0	41.0	31.0	41.0
35	36.700352695342524	39.0	35.0	41.0	31.0	41.0
36	36.5097241083701	39.0	35.0	41.0	30.0	41.0
37	36.44245440101553	39.0	35.0	41.0	30.0	41.0
38	36.394135757310984	39.0	35.0	40.0	30.0	41.0
39	36.340953771460015	39.0	35.0	40.0	30.0	41.0
40	36.21044619103455	39.0	35.0	40.0	30.0	41.0
41	36.08616152478141	38.0	35.0	40.0	30.0	41.0
42	36.06125647329387	38.0	35.0	40.0	30.0	41.0
43	36.03235101378016	38.0	35.0	40.0	30.0	41.0
44	35.91956166037798	38.0	35.0	40.0	29.0	41.0
45	35.95864768177256	38.0	35.0	40.0	30.0	41.0
46	35.86922709953159	38.0	35.0	40.0	29.0	41.0
47	35.65386553271113	38.0	34.0	40.0	28.0	41.0
48	35.53650391643294	38.0	34.0	40.0	28.0	41.0
49	35.55925910279581	38.0	34.0	40.0	28.0	41.0
50	35.45115432250141	37.0	34.0	40.0	28.0	41.0
51	34.461842341163454	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	18.0
10	28.0
11	30.0
12	26.0
13	23.0
14	43.0
15	68.0
16	117.0
17	242.0
18	497.0
19	853.0
20	1572.0
21	2529.0
22	3860.0
23	5843.0
24	8452.0
25	11750.0
26	15692.0
27	19987.0
28	23790.0
29	29284.0
30	36712.0
31	46801.0
32	62195.0
33	89965.0
34	146220.0
35	149357.0
36	159293.0
37	234825.0
38	393488.0
39	497331.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.82035720835918	4.6844700708687865	56.4532531843557	16.041919536416334
2	20.096885606361912	4.980443594504423	52.90280734364664	22.019863455487023
3	20.74684243389667	4.950562890638684	52.080933431973996	22.221661243490644
4	19.833162635519297	4.6649445764461746	51.142112627585455	24.359780160449077
5	19.45408984406394	5.51376264005292	50.50642641206933	24.525721103813808
6	22.04077994819304	6.573857887372414	52.592923837176954	18.792438327257592
7	84.64590335550001	2.0643444894830227	8.991361385475505	4.298390769541465
8	86.17579539342583	2.853040516173704	6.857724574096934	4.11343951630353
9	81.3189445723247	4.627696733523883	8.698478969136323	5.3548797250150955
10	42.6911798344403	27.905743895578272	15.466973579275567	13.936102690705866
11	29.498643003896856	23.639191531190303	27.39205338136227	19.47011208355057
12	27.811207530761667	21.033336561823177	31.412141457312835	19.743314450102314
13	26.601296616473952	21.050543725773448	33.131415335601524	19.21674432215108
14	20.57059779954375	23.36495879553974	34.582071989828194	21.482371415088313
15	18.51521752122045	23.81600286854757	36.89267469392886	20.77610491630312
16	24.775534091822372	24.33999707375176	29.975600859739977	20.90886797468589
17	25.11658626353132	24.520363184499953	28.05308667670933	22.309963875259395
18	24.6739912171338	23.71172951574713	30.94517818172826	20.66910108539081
19	25.0874783364897	25.530485530526747	28.719890039009776	20.66214609397378
20	23.744237660814857	29.460210236510925	28.662446961750636	18.13310514092358
21	24.080653171579122	25.79863908849428	31.883586838477278	18.237120901449316
22	23.159039531140845	22.7262329911821	32.57609790979325	21.538629567883806
23	22.238301704436562	24.048505655695983	31.38777322812578	22.325419411741674
24	21.880712108691576	26.43963170486932	29.478190177285306	22.2014660091538
25	23.369492419574538	26.015583302250533	27.327809868050934	23.287114410123994
26	21.271145749830506	26.587850299734374	27.302926454314463	24.83807749612066
27	20.382709994374185	25.263207786293208	31.89538456465875	22.458697654673855
28	18.52732435813157	25.186290733066397	31.26418045472249	25.02220445407954
29	20.80351273433169	25.51920298889468	30.08899297906496	23.588291297708665
30	23.643673636770163	24.197651582749973	31.08005349676361	21.078621283716252
31	23.152548205818288	25.13899679143063	30.287081438312836	21.421373564438255
32	25.18124192448219	24.6515806892345	27.64639999340564	22.520777392877676
33	24.852708737323884	23.168415889940093	29.16124864248871	22.81762673024731
34	21.719923010820935	23.292678403257618	31.578906695956626	23.40849188996482
35	21.878857444313702	25.19731568242376	28.86424474975426	24.05958212350828
36	24.31846235958645	24.621493911548995	29.80636273525902	21.253680993605528
37	21.38701075499265	26.00764946018963	30.689337534491607	21.916002250326112
38	21.49334484599073	25.32946051934724	29.463095269987615	23.71409936467441
39	21.222563846821206	23.716314658236872	29.98358622025582	25.077535274686095
40	24.38713646002271	22.67394175941706	29.668447831382156	23.270473949178076
41	21.494426733544493	22.848795395486572	28.82581198236832	26.830965888600616
42	20.310068972907473	22.681411935383494	31.583646393811193	25.424872697897843
43	20.5754405343082	22.51485277055947	32.37172419904258	24.53798249608975
44	22.3400506529449	22.207339113017067	29.79992292839141	25.652687305646626
45	22.09162866321974	22.0526807112844	28.326186006351193	27.529504619144667
46	22.364470400586896	22.702534501909273	30.078174103527367	24.85482099397646
47	19.16120742772476	23.725072795576832	33.25567784891904	23.858041927779368
48	19.458881060373447	24.573118082359464	31.98415086252197	23.983849994745118
49	22.141704601422322	21.84207326748588	32.637610944992716	23.378611186099086
50	21.102834957538487	22.32531637483179	29.561598555834674	27.01025011179505
51	19.845887693889704	22.970997170606456	28.63205107333549	28.55106406216835
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	260.0
1	1065.0
2	1870.0
3	5086.0
4	8302.0
5	5555.0
6	2808.0
7	2838.5
8	2869.0
9	3253.0
10	3637.0
11	3679.5
12	3722.0
13	3736.5
14	3751.0
15	4193.0
16	4635.0
17	4120.0
18	3605.0
19	4136.0
20	4667.0
21	5275.0
22	5883.0
23	7323.0
24	8763.0
25	10389.5
26	15736.5
27	19457.0
28	22337.0
29	25217.0
30	29715.5
31	34214.0
32	40382.5
33	46551.0
34	51266.5
35	55982.0
36	62686.0
37	69390.0
38	74027.0
39	78664.0
40	85724.0
41	92784.0
42	99472.0
43	106160.0
44	115443.0
45	124726.0
46	143929.0
47	163132.0
48	180366.0
49	197600.0
50	193422.5
51	189245.0
52	167208.0
53	145171.0
54	131166.5
55	117162.0
56	110020.0
57	102878.0
58	95165.5
59	87453.0
60	76136.5
61	64820.0
62	56918.0
63	49016.0
64	42879.0
65	36742.0
66	30410.5
67	24079.0
68	19935.5
69	15792.0
70	13700.0
71	11608.0
72	9498.5
73	7389.0
74	6010.0
75	3528.0
76	2425.0
77	1703.5
78	982.0
79	759.0
80	536.0
81	447.5
82	359.0
83	201.0
84	43.0
85	36.0
86	29.0
87	18.5
88	8.0
89	7.5
90	7.0
91	6.0
92	5.0
93	5.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1941052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.05522209009683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.3524884732551	17.626972232883837
2	12.305362112434775	6.166271612522327
3	4.946882522032845	3.7183572072945377
4	2.5089049232145864	2.5144468021631514
5	1.443841513842887	1.8087884896117574
6	0.9534440252712924	1.4333251082188874
7	0.6568074856104559	1.1519520195685635
8	0.4944424046589052	0.9910691407592318
9	0.3665339749320683	0.8265231130940058
>10	4.110382200416917	25.92533352521851
>50	1.7141355840259893	28.889523352722925
>100	0.13780985204455531	5.712934328594725
>500	0.005212167592784598	0.8142151051477047
>1k	0.0033357872593821423	1.8907112164509636
>5k	4.169734074227678E-4	0.5295767457488567
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	5109	0.26320778629320596	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5029	0.25908630989793163	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	4991	0.25712860861017633	No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT	4654	0.2397668892950833	No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	3892	0.20050982663009542	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2338	0.12045014765189187	No Hit
TCCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	2258	0.11632867125661754	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG	2183	0.11246478713604786	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2073	0.1067977570925457	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC	2022	0.10417031589055831	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC	1985	0.10226413305774394	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0303690988185788E-4	0.0	0.0	0.2620228618295646	0.0
2	1.0303690988185788E-4	0.0	0.0	1.075808376076478	0.0
3	1.0303690988185788E-4	0.0	0.0	1.6271588808542996	0.0
4	1.0303690988185788E-4	0.0	0.0	2.2934985770602747	0.0
5	1.0303690988185788E-4	0.0	0.0	3.3947055514226308	0.0
6	1.0303690988185788E-4	0.0	0.0	4.041262160931288	0.0
7	1.0303690988185788E-4	0.0	0.0	4.683233627950204	0.0
8	1.0303690988185788E-4	0.0	0.0	5.622724172252984	0.0
9	1.0303690988185788E-4	0.0	0.0	6.067173883028379	0.0
10	1.0303690988185788E-4	0.0	0.0	7.0234079251869606	0.0
11	1.0303690988185788E-4	0.0	0.0	8.793685073867161	0.0
12	1.0303690988185788E-4	0.0	0.0	10.355106406216835	0.0
13	1.0303690988185788E-4	0.0	0.0	10.813878247465807	0.0
14	1.0303690988185788E-4	0.0	0.0	10.994141321304118	0.0
15	1.0303690988185788E-4	0.0	0.0	11.256988478412737	0.0
16	1.0303690988185788E-4	0.0	0.0	11.811275535122192	0.0
17	1.0303690988185788E-4	0.0	0.0	12.62701875065686	0.0
18	1.5455536482278682E-4	0.0	0.0	13.48268876877075	0.0
19	1.5455536482278682E-4	0.0	0.0	14.159074563690204	0.0
20	1.5455536482278682E-4	0.0	0.0	14.839942464189521	0.0
21	1.5455536482278682E-4	0.0	0.0	15.802770868580543	0.0
22	1.5455536482278682E-4	0.0	0.0	16.812687140787574	0.0
23	1.5455536482278682E-4	0.0	0.0	17.72085446448627	0.0
24	1.5455536482278682E-4	0.0	0.0	18.434385065418134	0.0
25	1.5455536482278682E-4	0.0	0.0	19.055955224280442	0.0
26	1.5455536482278682E-4	0.0	0.0	19.609005838071315	0.0
27	2.0607381976371576E-4	0.0	0.0	20.2559745952195	0.0
28	2.575922747046447E-4	0.0	0.0	20.83329040128755	0.0
29	2.575922747046447E-4	0.0	0.0	21.531262428827254	0.0
30	2.575922747046447E-4	0.0	5.151845494092894E-5	22.28296820487035	0.0
31	2.575922747046447E-4	0.0	5.151845494092894E-5	22.92241526759716	0.0
32	2.575922747046447E-4	0.0	5.151845494092894E-5	23.509004395554577	0.0
33	2.575922747046447E-4	0.0	5.151845494092894E-5	24.124443858278912	0.0
34	2.575922747046447E-4	0.0	5.151845494092894E-5	24.778522162208947	0.0
35	2.575922747046447E-4	0.0	5.151845494092894E-5	25.488961655844356	0.0
36	2.575922747046447E-4	0.0	5.151845494092894E-5	26.1225356147079	0.0
37	3.0911072964557364E-4	0.0	5.151845494092894E-5	26.75698538730544	0.0
38	3.0911072964557364E-4	0.0	5.151845494092894E-5	27.490608185664268	0.0
39	3.0911072964557364E-4	0.0	5.151845494092894E-5	28.790367285368966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	55	1.8189894E-12	45.0	41
CTACGGT	20	7.0344907E-4	45.0	16
CTACCGT	20	7.0344907E-4	45.0	28
TACTACG	20	7.0344907E-4	45.0	8
TACGGGT	60	3.6379788E-12	41.250004	4
CGTTTTT	2565	0.0	40.964912	1
TTTGGGA	10345	0.0	39.54084	4
TTGGGAC	4135	0.0	38.52479	5
TGCGGGA	2325	0.0	38.225807	4
TTTGTCG	660	0.0	38.181816	1
TACCGGT	65	9.094947E-12	38.076927	40
TCTTGCG	635	0.0	37.913387	1
TCGTTCG	280	0.0	37.76786	1
TTTGGGC	3850	0.0	37.753246	4
TTGGGAT	6395	0.0	37.68178	5
GGTACCG	60	1.5643309E-10	37.500004	8
CGAACGA	60	1.5643309E-10	37.500004	22
GACCGTA	30	1.1402115E-4	37.500004	17
CGTTAGG	30	1.1402115E-4	37.500004	2
CGACTCG	30	1.1402115E-4	37.500004	30
>>END_MODULE