FastQCFastQC Report
Sat 14 Jan 2017
SRR2934431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041573
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82070.40199395270215665No Hit
TCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC44210.2165487102347063No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT43060.21091579874929772No Hit
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC41610.20381343209378258No Hit
CCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC27040.13244689266560639No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC21270.1041843715605565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAG302.1663345E-644.99999616
CGTTTTT41500.041.0421681
TTTGTCG5700.040.6578941
TTTCGCG5350.039.112151
TTGTTCG4950.038.6363641
CAACCCG356.2506715E-638.5714323
TGGGCGA26200.038.5591626
TTTGGGA123800.038.293624
CGCGCAT659.094947E-1238.0769236
CCCTCGT4050.037.7777814
GGCGATT19800.037.6136368
TTGGGAT66900.037.1973085
TTGGGAC48200.037.1576735
TTTGGGC51850.036.9720344
GGGCGAT40000.036.843757
CGTAGTA552.752131E-936.8181824
TCGTTTG7750.036.5806471
GGACGAT800.036.56258
CGTGCGG3450.036.5217362
TACGGGA3350.036.2686584
Produced by FastQC (version 0.11.5)