##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934431.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2041573
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21288290940368	33.0	31.0	34.0	30.0	34.0
2	32.40089088168779	34.0	31.0	34.0	30.0	34.0
3	32.4617855937554	34.0	31.0	34.0	30.0	34.0
4	35.9696753434729	37.0	35.0	37.0	35.0	37.0
5	35.92851835325016	37.0	35.0	37.0	35.0	37.0
6	35.568532205314234	37.0	35.0	37.0	33.0	37.0
7	35.896363245399506	37.0	35.0	37.0	35.0	37.0
8	35.99495291130907	37.0	35.0	37.0	35.0	37.0
9	37.78443337563731	39.0	38.0	39.0	35.0	39.0
10	37.23466611284534	39.0	37.0	39.0	34.0	39.0
11	37.12796162566805	39.0	37.0	39.0	34.0	39.0
12	37.005902801418316	39.0	37.0	39.0	33.0	39.0
13	36.96717873913889	39.0	37.0	39.0	33.0	39.0
14	38.161464223909704	40.0	37.0	41.0	33.0	41.0
15	38.18319697605719	40.0	37.0	41.0	33.0	41.0
16	38.20072904569173	40.0	37.0	41.0	33.0	41.0
17	38.13442722841652	40.0	37.0	41.0	33.0	41.0
18	38.08501336959296	40.0	37.0	41.0	33.0	41.0
19	38.10879258297401	40.0	37.0	41.0	34.0	41.0
20	38.04466653898734	40.0	37.0	41.0	34.0	41.0
21	37.89873445622567	40.0	36.0	41.0	33.0	41.0
22	37.959380830369525	40.0	37.0	41.0	33.0	41.0
23	37.910077670502105	40.0	36.0	41.0	33.0	41.0
24	37.859309463830094	40.0	36.0	41.0	33.0	41.0
25	37.78245989734386	40.0	36.0	41.0	33.0	41.0
26	37.723711079643	40.0	36.0	41.0	33.0	41.0
27	37.63920810081246	40.0	36.0	41.0	33.0	41.0
28	37.54080407607272	40.0	36.0	41.0	33.0	41.0
29	37.46790048653661	39.0	36.0	41.0	33.0	41.0
30	37.41190444818775	39.0	36.0	41.0	32.0	41.0
31	37.356266467082	39.0	36.0	41.0	32.0	41.0
32	37.2119135588098	39.0	35.0	41.0	32.0	41.0
33	37.1131730288361	39.0	35.0	41.0	31.0	41.0
34	36.98494739105582	39.0	35.0	41.0	31.0	41.0
35	36.905898539998326	39.0	35.0	41.0	31.0	41.0
36	36.737677271398084	39.0	35.0	41.0	31.0	41.0
37	36.73074242263196	39.0	35.0	41.0	31.0	41.0
38	36.65378999428382	39.0	35.0	41.0	30.0	41.0
39	36.54542257367236	39.0	35.0	41.0	30.0	41.0
40	36.45558988093985	39.0	35.0	41.0	30.0	41.0
41	36.374787969864414	39.0	35.0	41.0	30.0	41.0
42	36.29914139734411	39.0	35.0	40.0	30.0	41.0
43	36.24806901345188	39.0	35.0	40.0	30.0	41.0
44	36.127847987801566	39.0	35.0	40.0	30.0	41.0
45	36.12966717330215	38.0	35.0	40.0	30.0	41.0
46	36.021165052633435	38.0	35.0	40.0	29.0	41.0
47	35.83892567152877	38.0	35.0	40.0	29.0	41.0
48	35.72584129982126	38.0	35.0	40.0	28.0	41.0
49	35.70880394676066	38.0	35.0	40.0	28.0	41.0
50	35.61825122099479	38.0	34.0	40.0	28.0	41.0
51	34.65959042365862	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	20.0
10	39.0
11	32.0
12	27.0
13	28.0
14	33.0
15	53.0
16	90.0
17	196.0
18	372.0
19	799.0
20	1318.0
21	2190.0
22	3582.0
23	5298.0
24	7961.0
25	11774.0
26	16790.0
27	20605.0
28	24065.0
29	27746.0
30	34495.0
31	43098.0
32	55716.0
33	76731.0
34	125890.0
35	153793.0
36	173077.0
37	259403.0
38	438987.0
39	557192.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.56800369127139	4.299723791409859	56.27547974037667	15.856792776942093
2	20.345488503227656	4.201417240529729	54.15623149404895	21.296862762193662
3	20.383792301328437	4.295511353255554	52.802128554795736	22.518567790620274
4	18.703617259828572	4.752952747709731	51.64444278994678	24.898987202514924
5	17.666328855250338	5.818454691554012	51.694747138603425	24.820469314592227
6	22.151350943610638	6.5442186000696525	53.574082337491724	17.730348118827983
7	84.45718081107067	2.315469493375941	8.938744781597327	4.2886049139560525
8	86.96921442436788	2.4206335017165683	6.931420037392735	3.6787320365228187
9	81.90718627254573	4.888191605198541	8.591708452257157	4.612913669998575
10	38.622718854530305	29.34364825553629	17.043867645193192	14.989765244740209
11	26.147583260554484	26.53302135167344	27.27730039533242	20.04209499243965
12	24.087211184709044	23.0884225055876	31.692866235985683	21.131500073717667
13	23.46426015626186	22.40757494343822	33.298001100132105	20.83016380016781
14	20.38668222982965	24.687140748824557	32.72932194930086	22.196855072044936
15	19.391616170472474	25.5369756555362	34.1211409045868	20.950267269404524
16	22.395770320238366	25.608048303930353	30.94143584383218	21.0547455319991
17	22.18627499482017	25.91041319609928	29.969832085357712	21.933479723722833
18	22.12808457008395	24.715501233607615	31.60768681795851	21.54872737834993
19	23.47596681578371	25.927899712623553	29.61353818844587	20.982595283146868
20	24.397658080313562	26.757652065343734	29.55147819842837	19.293211655914337
21	25.064888691219956	25.595264043950422	30.070930601061047	19.26891666376857
22	24.07153699622791	24.12962945728612	29.964786955940347	21.834046590545622
23	22.563827009859555	24.81841207735408	29.565829877256412	23.051931035529957
24	22.394692719780288	24.46123650734017	30.39592510284962	22.748145670029924
25	22.316076868179586	25.84624698700463	28.44115787189584	23.39651827291995
26	21.238329464584417	27.157980635519767	28.309151815781263	23.294538084114553
27	20.625076840259936	26.499223882760987	29.84243032210947	23.033268954869605
28	19.394261189778668	26.22198667400088	30.376920149316238	24.006831986904213
29	20.896387246500613	24.6091126792919	30.38921459090613	24.105285483301355
30	22.11481049171399	25.280996564903628	29.572393443682888	23.031799499699495
31	22.34071473319837	26.690742873264877	27.86478857234103	23.103753821195717
32	22.616629432305384	27.11375003489956	27.48601201132656	22.783608521468494
33	22.362658597071963	27.052326808789108	27.33568674742466	23.249327846714273
34	21.34550172832419	25.976636642432084	28.66657229499019	24.01128933425354
35	20.945467049182177	26.596452833183044	28.11239176850399	24.345688349130793
36	20.955018507787866	28.506499645126578	27.33255190972843	23.205929937357126
37	21.72437625301667	27.819088516550718	27.93669391199825	22.519841318434363
38	22.134158318120388	27.286068144514058	28.038037336896597	22.54173620046895
39	22.423738950309392	26.172857889480316	28.712419296297515	22.690983863912777
40	22.546487438852296	25.345554628710314	29.41031253842013	22.69764539401726
41	20.574625546086278	27.481505682138234	27.525638319080436	24.418230452695056
42	20.69267177808484	27.693597045023616	27.041942658920355	24.57178851797119
43	21.08246925287511	26.34042476071147	27.846469364553705	24.730636621859713
44	21.494308555216982	25.029278894264372	27.85366969488723	25.62274285563142
45	22.574309123406312	24.52324751551867	27.253887076288724	25.648556284786288
46	22.307603010031972	25.530559034626734	27.842158962721392	24.319678992619906
47	20.236846784317777	26.238983372135117	29.310144677657863	24.214025165889243
48	20.29533110008802	25.772284410109265	29.36324099113772	24.569143498665
49	21.74372407942307	25.009490231306934	29.69475007751376	23.552035611756228
50	21.8020124678373	24.08613358425097	28.866222270768667	25.245631677143066
51	20.67072791421125	24.45565257769377	28.15387938614	26.71974012195498
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	350.0
1	1064.5
2	1779.0
3	7763.0
4	13747.0
5	9159.5
6	4572.0
7	4551.5
8	4531.0
9	4710.0
10	4889.0
11	4987.0
12	5085.0
13	5202.0
14	5319.0
15	5295.0
16	5271.0
17	5285.5
18	5300.0
19	5631.0
20	5962.0
21	6987.0
22	8012.0
23	9189.5
24	10367.0
25	11714.5
26	17449.0
27	21836.0
28	24230.5
29	26625.0
30	30975.5
31	35326.0
32	41884.5
33	48443.0
34	54375.5
35	60308.0
36	67133.5
37	73959.0
38	79224.5
39	84490.0
40	91821.5
41	99153.0
42	107099.0
43	115045.0
44	129506.0
45	143967.0
46	155792.5
47	167618.0
48	187134.0
49	206650.0
50	197409.5
51	188169.0
52	169643.0
53	151117.0
54	135128.5
55	119140.0
56	109871.5
57	100603.0
58	93417.0
59	86231.0
60	78766.0
61	71301.0
62	61092.5
63	50884.0
64	43201.0
65	35518.0
66	29853.5
67	24189.0
68	20207.0
69	16225.0
70	13759.0
71	11293.0
72	9015.5
73	6738.0
74	5315.5
75	3075.5
76	2258.0
77	1718.0
78	1178.0
79	895.0
80	612.0
81	493.5
82	375.0
83	233.5
84	92.0
85	55.0
86	18.0
87	13.5
88	9.0
89	28.0
90	47.0
91	31.0
92	15.0
93	8.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2041573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.615452802160483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9325533870303	20.140654858005846
2	11.18905312926157	6.179815371839778
3	4.1896617723547624	3.470982207944369
4	2.081129738849298	2.298853603134614
5	1.1705165206930197	1.6162171865673591
6	0.7545335702047217	1.250207171738048
7	0.5664123818595614	1.0949214078461416
8	0.4456555273311956	0.9845583344829258
9	0.332936143970419	0.827476413295357
>10	5.127931380145495	36.65454046996762
>50	1.1338395907814804	19.91046378602026
>100	0.07090551993741284	3.3960031638402297
>500	0.0028867185663850625	0.5295769066215734
>1k	0.001804199103990664	1.2368253956133164
>5k	1.804199103990664E-4	0.40890372308243983
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8207	0.40199395270215665	No Hit
TCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	4421	0.2165487102347063	No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT	4306	0.21091579874929772	No Hit
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	4161	0.20381343209378258	No Hit
CCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	2704	0.13244689266560639	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2127	0.1041843715605565	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.898183900355265E-5	0.0	0.0	0.23163511664780048	0.0
2	4.898183900355265E-5	0.0	0.0	0.8609048023264414	0.0
3	4.898183900355265E-5	0.0	0.0	1.2753401421355004	0.0
4	4.898183900355265E-5	0.0	0.0	1.778628537897004	0.0
5	4.898183900355265E-5	0.0	0.0	2.607940054066154	0.0
6	4.898183900355265E-5	0.0	0.0	3.2274623537830878	0.0
7	4.898183900355265E-5	0.0	0.0	3.753086468130211	0.0
8	4.898183900355265E-5	0.0	0.0	4.560062265713741	0.0
9	4.898183900355265E-5	0.0	0.0	4.979346807584152	0.0
10	4.898183900355265E-5	0.0	0.0	5.753161900162277	0.0
11	9.79636780071053E-5	0.0	0.0	7.1722637397732045	0.0
12	9.79636780071053E-5	0.0	0.0	8.363061227788572	0.0
13	1.4694551701065797E-4	0.0	0.0	8.740172406276924	0.0
14	1.4694551701065797E-4	0.0	0.0	8.883689194557334	0.0
15	1.4694551701065797E-4	0.0	0.0	9.08877615446521	0.0
16	1.4694551701065797E-4	0.0	0.0	9.582855964494044	0.0
17	1.959273560142106E-4	0.0	0.0	10.316016130699222	0.0
18	1.959273560142106E-4	0.0	0.0	11.09443551614368	0.0
19	1.959273560142106E-4	0.0	0.0	11.692650715894068	0.0
20	1.959273560142106E-4	0.0	0.0	12.308254468490718	0.0
21	1.959273560142106E-4	0.0	0.0	13.159607812211465	0.0
22	2.4490919501776325E-4	0.0	0.0	14.083356313979467	0.0
23	2.4490919501776325E-4	0.0	0.0	14.989030517155154	0.0
24	2.9389103402131594E-4	0.0	0.0	15.675804881823966	0.0
25	2.9389103402131594E-4	0.0	0.0	16.282004121331934	4.898183900355265E-5
26	2.9389103402131594E-4	0.0	0.0	16.821686023473077	4.898183900355265E-5
27	2.9389103402131594E-4	0.0	0.0	17.344910027709027	4.898183900355265E-5
28	3.428728730248686E-4	0.0	0.0	17.913197323828243	4.898183900355265E-5
29	3.428728730248686E-4	0.0	0.0	18.51880878126817	4.898183900355265E-5
30	3.428728730248686E-4	0.0	0.0	19.173549023228656	4.898183900355265E-5
31	3.428728730248686E-4	0.0	0.0	19.7904263036394	4.898183900355265E-5
32	3.428728730248686E-4	0.0	0.0	20.366795603194205	4.898183900355265E-5
33	3.428728730248686E-4	0.0	0.0	20.96471691191057	4.898183900355265E-5
34	3.918547120284212E-4	0.0	0.0	21.563715821085015	4.898183900355265E-5
35	3.918547120284212E-4	0.0	0.0	22.16766189599882	4.898183900355265E-5
36	4.898183900355265E-4	0.0	0.0	22.76200753046793	4.898183900355265E-5
37	4.898183900355265E-4	0.0	0.0	23.349054870925507	4.898183900355265E-5
38	4.898183900355265E-4	0.0	0.0	23.970977280753615	4.898183900355265E-5
39	4.898183900355265E-4	0.0	0.0	24.591136344377595	4.898183900355265E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAG	30	2.1663345E-6	44.999996	16
CGTTTTT	4150	0.0	41.042168	1
TTTGTCG	570	0.0	40.657894	1
TTTCGCG	535	0.0	39.11215	1
TTGTTCG	495	0.0	38.636364	1
CAACCCG	35	6.2506715E-6	38.57143	23
TGGGCGA	2620	0.0	38.559162	6
TTTGGGA	12380	0.0	38.29362	4
CGCGCAT	65	9.094947E-12	38.07692	36
CCCTCGT	405	0.0	37.77778	14
GGCGATT	1980	0.0	37.613636	8
TTGGGAT	6690	0.0	37.197308	5
TTGGGAC	4820	0.0	37.157673	5
TTTGGGC	5185	0.0	36.972034	4
GGGCGAT	4000	0.0	36.84375	7
CGTAGTA	55	2.752131E-9	36.81818	24
TCGTTTG	775	0.0	36.580647	1
GGACGAT	80	0.0	36.5625	8
CGTGCGG	345	0.0	36.521736	2
TACGGGA	335	0.0	36.268658	4
>>END_MODULE