FastQCFastQC Report
Sat 14 Jan 2017
SRR2934430.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934430.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677714
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101371.4957636997317452No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT29130.4298273312931413Illumina PCR Primer Index 10 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC25670.37877334686903325No Hit
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC25150.3711004937185893Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC24520.3618045370170898Illumina PCR Primer Index 10 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT23700.34970503781831275No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT18900.2788787010449836No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCC18720.2762227134159837No Hit
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC15150.22354562544082018Illumina PCR Primer Index 10 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTC12090.17839383574782283No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG11310.16688455602215682Illumina PCR Primer Index 10 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG10760.15876903826687955Illumina PCR Primer Index 10 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT10380.15316195327232432Illumina PCR Primer Index 10 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGT10290.15183395945782438No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT9710.14327577709771377Illumina PCR Primer Index 10 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG9130.13471759473760317No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC8350.12320831501193719No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA253.8891063E-545.020
CTATCGG207.0312363E-445.02
CCTACGG207.0312363E-445.02
TGTCGCG406.8084773E-945.01
GCGCGAC302.164179E-644.9999969
CGACCCG302.164179E-644.99999632
CGCGACC302.164179E-644.99999610
TATTGCG302.164179E-644.9999961
GGCGCGA302.164179E-644.9999968
CGTTTTT67200.044.497771
TTTCGCG2050.040.609761
TTTACCG501.0804797E-940.51
TTACCGG501.0804797E-940.52
TTCGTCG451.9263098E-840.0000041
CTACGGG900.040.0000043
TGGGCGA8750.039.8571436
TTGGGAC16600.039.4427725
CTTCGTG403.455425E-739.3751
TCTTGCG1950.039.230771
CGGGTGC750.039.06
Produced by FastQC (version 0.11.5)