##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934430.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 677714 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14452999347807 33.0 31.0 34.0 30.0 34.0 2 32.38238549004447 34.0 31.0 34.0 30.0 34.0 3 32.350025231882476 34.0 31.0 34.0 30.0 34.0 4 35.93926789176555 37.0 35.0 37.0 35.0 37.0 5 35.93737476280555 37.0 35.0 37.0 35.0 37.0 6 35.65037021516451 37.0 35.0 37.0 35.0 37.0 7 35.963189487010744 37.0 35.0 37.0 35.0 37.0 8 36.04814715351904 37.0 35.0 37.0 35.0 37.0 9 37.848207355905295 39.0 38.0 39.0 35.0 39.0 10 37.330305999285834 39.0 37.0 39.0 34.0 39.0 11 37.21981543837076 39.0 37.0 39.0 34.0 39.0 12 37.192476472376256 39.0 37.0 39.0 34.0 39.0 13 37.18567124185128 39.0 37.0 39.0 33.0 39.0 14 38.42622551695848 40.0 38.0 41.0 34.0 41.0 15 38.43755625529353 40.0 38.0 41.0 34.0 41.0 16 38.426005660204744 40.0 38.0 41.0 34.0 41.0 17 38.360143069200284 40.0 38.0 41.0 34.0 41.0 18 38.17557111111767 39.0 38.0 41.0 34.0 41.0 19 38.0805531536902 40.0 37.0 41.0 34.0 41.0 20 37.88165214234913 39.0 36.0 41.0 34.0 41.0 21 37.7307787650838 39.0 35.0 41.0 33.0 41.0 22 37.81735363294841 39.0 36.0 41.0 33.0 41.0 23 37.74960529072736 39.0 35.0 41.0 33.0 41.0 24 37.67477726592634 39.0 35.0 41.0 33.0 41.0 25 37.60705991022762 39.0 35.0 41.0 33.0 41.0 26 37.54632042424976 39.0 35.0 41.0 33.0 41.0 27 37.47178750918529 39.0 35.0 41.0 33.0 41.0 28 37.34929631083318 39.0 35.0 41.0 33.0 41.0 29 37.220340733701825 39.0 35.0 41.0 32.0 41.0 30 37.117644020929184 39.0 35.0 41.0 32.0 41.0 31 36.93261169165754 39.0 35.0 41.0 31.0 41.0 32 36.579973263057866 39.0 35.0 41.0 30.0 41.0 33 36.256620049165285 39.0 35.0 41.0 30.0 41.0 34 35.92456404914167 39.0 35.0 41.0 27.0 41.0 35 35.7101476434012 39.0 35.0 41.0 26.0 41.0 36 35.488741563550406 39.0 35.0 41.0 25.0 41.0 37 35.404251645974554 39.0 35.0 41.0 24.0 41.0 38 35.28040589393166 39.0 35.0 41.0 24.0 41.0 39 35.15599058009721 39.0 35.0 40.0 23.0 41.0 40 35.05512797433726 39.0 35.0 40.0 23.0 41.0 41 34.96215807848149 38.0 35.0 40.0 22.0 41.0 42 34.87187220567968 38.0 34.0 40.0 22.0 41.0 43 34.78232263167059 38.0 34.0 40.0 22.0 41.0 44 34.59006749159675 38.0 34.0 40.0 21.0 41.0 45 34.60123296847933 38.0 34.0 40.0 21.0 41.0 46 34.48378371997627 38.0 34.0 40.0 20.0 41.0 47 34.27103615979602 38.0 33.0 40.0 20.0 41.0 48 34.13643660895304 38.0 33.0 40.0 20.0 41.0 49 34.09542815996128 38.0 33.0 40.0 20.0 41.0 50 33.94194011043006 38.0 33.0 40.0 19.0 41.0 51 33.06811575384307 36.0 32.0 40.0 17.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 7.0 10 17.0 11 6.0 12 3.0 13 10.0 14 18.0 15 25.0 16 36.0 17 79.0 18 190.0 19 430.0 20 711.0 21 1208.0 22 1937.0 23 3184.0 24 5212.0 25 9317.0 26 13395.0 27 14036.0 28 12244.0 29 12157.0 30 12939.0 31 14727.0 32 18349.0 33 23887.0 34 35406.0 35 46817.0 36 56368.0 37 82941.0 38 140452.0 39 171552.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.28805661385186 3.982948559421821 53.979112132846595 21.74988269387972 2 27.144193568378398 3.5492848015534575 48.63349436487958 20.67302726518856 3 17.00746922743222 3.5576954290452907 57.99171922079225 21.44311612273024 4 15.865837211567122 3.791717450133832 57.20761265076418 23.13483268753486 5 16.015162738264223 4.76484180642572 56.8530383022927 22.36695715301735 6 18.860463263264446 5.1869962845684165 59.04496587055896 16.90757458160817 7 76.30313081919512 1.3238622781881442 17.964362548213554 4.408644354403185 8 78.35015950681262 1.4283311249288049 15.829686268839069 4.391823099419519 9 73.41282605937018 4.075022797227149 17.60137757225024 4.910773571152434 10 31.308191951177044 30.317508565560104 24.677371280510656 13.696928202752193 11 19.657259551964398 25.398471921784115 36.295398944097364 18.648869582154123 12 19.01244477759055 21.613837105327615 39.60136576786078 19.77235234922106 13 19.09743638171854 22.59301121121889 40.2218339889688 18.08771841809377 14 17.801019309030064 24.84469850113765 38.36913506287313 18.98514712695916 15 16.134387071832663 26.479606441655328 38.69301799874283 18.692988487769178 16 18.179645101030818 26.81676931567003 35.91647804236005 19.0871075409391 17 18.513414213075134 26.488017069147162 34.53993867619675 20.45863004158096 18 18.692693378032622 25.553256978607497 36.306465559218196 19.44758408414169 19 18.8434944534125 27.579923094402652 34.34900267664531 19.227579775539535 20 20.3594731700983 27.697671879288315 34.33660806770998 17.606246882903406 21 20.37791752863302 27.43989352440705 35.15598025125643 17.0262086957035 22 19.89201934739433 25.492169263140497 34.14537695842199 20.470434431043184 23 18.494084525330745 26.330871134431337 34.09210965097371 21.082934689264203 24 18.99562352260688 26.804374706734702 34.29971935064054 19.900282420017884 25 18.24220836518059 28.24878931230578 32.85884606190812 20.650156260605506 26 18.273785106992037 28.512617416786433 33.029714599373776 20.183882876847754 27 18.83965802683728 26.898662267564195 33.66670896572891 20.59497073986962 28 17.976904712017163 26.224779184139624 34.6238973962468 21.17441870759642 29 17.63324942379824 24.64225322186055 35.87531023411055 21.849187120230656 30 18.978949822491494 25.268328527963124 34.79697925673661 20.955742392808766 31 19.162212968892483 26.77574906228881 32.97866061494967 21.083377353869036 32 18.120180489114876 26.7698468675577 34.02246375314661 21.087508890180814 33 18.38046727675686 26.066600365345856 33.43091628622103 22.122016071676253 34 17.536748539944575 25.498071457871607 34.051384507329054 22.91379549485476 35 17.15620453465621 25.667021782049655 33.86177650159212 23.314997181702015 36 18.433291919600304 26.4047961234385 32.268921698533596 22.892990258427595 37 18.47209884995736 27.352983706991445 32.04567118282933 22.129246260221862 38 17.977347376621996 27.871639068987804 32.61611830359119 21.53489525079901 39 18.693873816978844 27.05418509872896 32.40452462248087 21.847416461811324 40 18.94014289213444 27.306651478352222 32.0664764192565 21.686729210256832 41 17.66895770192146 27.87134395925125 31.126256798590557 23.333441540236738 42 18.54454829028174 27.823831291665808 30.611880527774254 23.019739890278203 43 19.49111277028363 27.62064823804732 30.476130048958705 22.412108942710347 44 19.159852091000037 27.66742313129137 29.683465296570528 23.48925948113806 45 19.359198718043306 26.704037396305818 29.685531064726415 24.25123282092446 46 19.554266253906516 27.339851323714726 30.410025467970264 22.695856954408498 47 17.5591768799228 27.91664330381252 31.752036994956573 22.7721428213081 48 17.31969532870798 27.181229840316124 32.035489896918165 23.463584934057728 49 18.770602348483283 26.085044723880575 32.1116282089495 23.03272471868664 50 18.184661966552262 25.836414770832533 31.888082583508677 24.090840679106527 51 17.404539377967698 25.87330348790198 30.556695007038364 26.16546212709196 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 68.0 1 233.0 2 398.0 3 7589.5 4 14781.0 5 10022.5 6 5264.0 7 5371.0 8 5478.0 9 5924.5 10 6371.0 11 6724.0 12 7077.0 13 7263.0 14 7449.0 15 7517.5 16 7586.0 17 7437.0 18 7288.0 19 7213.5 20 7139.0 21 7133.0 22 7127.0 23 7400.0 24 7673.0 25 8087.0 26 9075.0 27 9649.0 28 10252.0 29 10855.0 30 12300.0 31 13745.0 32 15062.5 33 16380.0 34 18376.0 35 20372.0 36 22304.0 37 24236.0 38 26088.5 39 27941.0 40 30517.5 41 33094.0 42 36507.5 43 39921.0 44 44527.5 45 49134.0 46 56999.0 47 64864.0 48 69287.5 49 73711.0 50 66950.0 51 60189.0 52 50254.0 53 40319.0 54 35018.0 55 29717.0 56 25933.5 57 22150.0 58 19212.0 59 16274.0 60 14142.0 61 12010.0 62 10151.0 63 8292.0 64 6748.5 65 5205.0 66 4068.5 67 2932.0 68 2401.5 69 1871.0 70 1411.0 71 951.0 72 820.5 73 690.0 74 582.0 75 367.0 76 260.0 77 209.0 78 158.0 79 124.5 80 91.0 81 56.5 82 22.0 83 11.5 84 1.0 85 1.5 86 2.0 87 1.5 88 1.0 89 0.5 90 0.0 91 1.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 677714.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.35680990612592 #Duplication Level Percentage of deduplicated Percentage of total 1 77.1800376672785 37.32180410024225 2 9.284251191127249 8.979135399401269 3 3.783119758452966 5.488188090348572 4 2.1849455744393578 4.226279911935805 5 1.5804548152653408 3.821287653350372 6 1.1492325571954702 3.3343932183739406 7 0.907956631273566 3.0734120355051604 8 0.6766119711359107 2.6175037174742712 9 0.5651252389967566 2.459488837978813 >10 2.6121459500793636 19.351104400068543 >50 0.04603951208776762 1.5302264792168478 >100 0.024247464089329075 2.2197922600737314 >500 0.0015346496259062386 0.5735007550764141 >1k 0.003990089027356221 3.4993341880011943 >5k 0.0 0.0 >10k+ 3.0692992518124775E-4 1.5045489529527976 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10137 1.4957636997317452 No Hit CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT 2913 0.4298273312931413 Illumina PCR Primer Index 10 (95% over 24bp) CGTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC 2567 0.37877334686903325 No Hit TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC 2515 0.3711004937185893 Illumina PCR Primer Index 10 (95% over 23bp) CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC 2452 0.3618045370170898 Illumina PCR Primer Index 10 (95% over 23bp) CGTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT 2370 0.34970503781831275 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT 1890 0.2788787010449836 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCC 1872 0.2762227134159837 No Hit GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC 1515 0.22354562544082018 Illumina PCR Primer Index 10 (95% over 23bp) CGTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTC 1209 0.17839383574782283 No Hit CGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG 1131 0.16688455602215682 Illumina PCR Primer Index 10 (95% over 22bp) TCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG 1076 0.15876903826687955 Illumina PCR Primer Index 10 (95% over 22bp) CGTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT 1038 0.15316195327232432 Illumina PCR Primer Index 10 (95% over 21bp) CGTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGT 1029 0.15183395945782438 No Hit TTCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT 971 0.14327577709771377 Illumina PCR Primer Index 10 (95% over 21bp) CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG 913 0.13471759473760317 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC 835 0.12320831501193719 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.475548682777691E-4 0.0 0.0 0.4730609076985277 0.0 2 1.475548682777691E-4 0.0 0.0 1.5677704754512967 0.0 3 1.475548682777691E-4 0.0 0.0 2.312627450517475 0.0 4 1.475548682777691E-4 0.0 0.0 3.3825478003995784 0.0 5 1.475548682777691E-4 0.0 0.0 5.260773718707301 0.0 6 1.475548682777691E-4 0.0 0.0 6.8477263270347075 0.0 7 1.475548682777691E-4 0.0 0.0 8.043215869821193 0.0 8 1.475548682777691E-4 0.0 0.0 9.41842724217 0.0 9 1.475548682777691E-4 0.0 0.0 10.253587796622174 0.0 10 1.475548682777691E-4 0.0 0.0 11.453799095193547 0.0 11 1.475548682777691E-4 0.0 0.0 13.300153161953272 0.0 12 1.475548682777691E-4 0.0 0.0 14.657067730635637 0.0 13 1.475548682777691E-4 0.0 0.0 15.232089052314103 0.0 14 1.475548682777691E-4 0.0 0.0 15.456224897228035 0.0 15 1.475548682777691E-4 0.0 0.0 15.731414726566074 0.0 16 1.475548682777691E-4 0.0 0.0 16.290647677338818 0.0 17 2.951097365555382E-4 0.0 0.0 17.140268608882213 0.0 18 2.951097365555382E-4 0.0 0.0 18.059535438252713 0.0 19 2.951097365555382E-4 0.0 0.0 18.634114095326346 0.0 20 2.951097365555382E-4 0.0 0.0 19.21326695331659 0.0 21 2.951097365555382E-4 0.0 0.0 19.942483112345325 0.0 22 2.951097365555382E-4 0.0 0.0 20.68615964846528 0.0 23 2.951097365555382E-4 0.0 0.0 21.449756091802737 0.0 24 2.951097365555382E-4 0.0 0.0 22.028023620583316 0.0 25 2.951097365555382E-4 0.0 0.0 22.521151990367617 0.0 26 2.951097365555382E-4 0.0 0.0 22.97798186255559 0.0 27 2.951097365555382E-4 0.0 0.0 23.42920464974901 0.0 28 2.951097365555382E-4 0.0 0.0 23.902265557447535 0.0 29 2.951097365555382E-4 0.0 0.0 24.379162891721286 0.0 30 2.951097365555382E-4 0.0 0.0 24.900179131610088 0.0 31 2.951097365555382E-4 0.0 0.0 25.38637242258534 0.0 32 2.951097365555382E-4 0.0 0.0 25.867991512643975 0.0 33 2.951097365555382E-4 0.0 0.0 26.327329817592673 0.0 34 2.951097365555382E-4 0.0 0.0 26.771765080845313 0.0 35 2.951097365555382E-4 0.0 0.0 27.233169153949895 0.0 36 4.4266460483330725E-4 0.0 0.0 27.683506611933648 0.0 37 4.4266460483330725E-4 0.0 0.0 28.13768049649262 0.0 38 4.4266460483330725E-4 0.0 0.0 28.57901710751143 0.0 39 4.4266460483330725E-4 0.0 0.0 29.05266823468307 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACGA 25 3.8891063E-5 45.0 20 CTATCGG 20 7.0312363E-4 45.0 2 CCTACGG 20 7.0312363E-4 45.0 2 TGTCGCG 40 6.8084773E-9 45.0 1 GCGCGAC 30 2.164179E-6 44.999996 9 CGACCCG 30 2.164179E-6 44.999996 32 CGCGACC 30 2.164179E-6 44.999996 10 TATTGCG 30 2.164179E-6 44.999996 1 GGCGCGA 30 2.164179E-6 44.999996 8 CGTTTTT 6720 0.0 44.49777 1 TTTCGCG 205 0.0 40.60976 1 TTTACCG 50 1.0804797E-9 40.5 1 TTACCGG 50 1.0804797E-9 40.5 2 TTCGTCG 45 1.9263098E-8 40.000004 1 CTACGGG 90 0.0 40.000004 3 TGGGCGA 875 0.0 39.857143 6 TTGGGAC 1660 0.0 39.442772 5 CTTCGTG 40 3.455425E-7 39.375 1 TCTTGCG 195 0.0 39.23077 1 CGGGTGC 75 0.0 39.0 6 >>END_MODULE