##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934429.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2029320
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09962253365659	33.0	31.0	34.0	30.0	34.0
2	32.32390406638677	34.0	31.0	34.0	30.0	34.0
3	32.32354039776871	34.0	31.0	34.0	30.0	34.0
4	35.89588729229496	37.0	35.0	37.0	35.0	37.0
5	35.88643141544951	37.0	35.0	37.0	35.0	37.0
6	35.58553949106105	37.0	35.0	37.0	33.0	37.0
7	35.93583121439694	37.0	35.0	37.0	35.0	37.0
8	36.02620483708829	37.0	35.0	37.0	35.0	37.0
9	37.81368832909546	39.0	38.0	39.0	35.0	39.0
10	37.209722468610174	39.0	37.0	39.0	34.0	39.0
11	37.10472276427572	39.0	37.0	39.0	33.0	39.0
12	37.07878156229673	39.0	37.0	39.0	33.0	39.0
13	37.08700155717186	39.0	37.0	39.0	33.0	39.0
14	38.31476356612067	40.0	38.0	41.0	33.0	41.0
15	38.312142491080756	40.0	38.0	41.0	33.0	41.0
16	38.308827094790374	40.0	38.0	41.0	34.0	41.0
17	38.23129274830978	40.0	38.0	41.0	33.0	41.0
18	38.09819348353143	39.0	37.0	41.0	33.0	41.0
19	38.02737912207045	40.0	37.0	41.0	34.0	41.0
20	37.8651903100546	39.0	36.0	41.0	34.0	41.0
21	37.72313139376737	39.0	36.0	41.0	33.0	41.0
22	37.79827134212446	39.0	36.0	41.0	33.0	41.0
23	37.7347717462007	39.0	36.0	41.0	33.0	41.0
24	37.67409723454162	39.0	36.0	41.0	33.0	41.0
25	37.62075473557645	39.0	36.0	41.0	33.0	41.0
26	37.57080943370193	39.0	36.0	41.0	33.0	41.0
27	37.48037076459109	39.0	35.0	41.0	33.0	41.0
28	37.35418711686673	39.0	35.0	41.0	33.0	41.0
29	37.26077750182327	39.0	35.0	41.0	32.0	41.0
30	37.1638903672166	39.0	35.0	41.0	32.0	41.0
31	37.00716249778251	39.0	35.0	41.0	31.0	41.0
32	36.71809473124002	39.0	35.0	41.0	31.0	41.0
33	36.4391446395837	39.0	35.0	41.0	30.0	41.0
34	36.1390593893521	39.0	35.0	41.0	29.0	41.0
35	35.98387489405318	39.0	35.0	41.0	28.0	41.0
36	35.77244200027595	39.0	35.0	41.0	27.0	41.0
37	35.69576163443912	39.0	35.0	41.0	26.0	41.0
38	35.61503163621312	39.0	35.0	41.0	26.0	41.0
39	35.55912029645398	39.0	35.0	40.0	25.0	41.0
40	35.48431691404017	39.0	35.0	40.0	25.0	41.0
41	35.41892013088128	39.0	35.0	40.0	24.0	41.0
42	35.352883231821494	39.0	35.0	40.0	24.0	41.0
43	35.29377870419648	39.0	35.0	40.0	24.0	41.0
44	35.13366842094889	38.0	34.0	40.0	23.0	41.0
45	35.167796109041454	38.0	34.0	40.0	23.0	41.0
46	35.043407643939844	38.0	34.0	40.0	23.0	41.0
47	34.82131699288431	38.0	34.0	40.0	23.0	41.0
48	34.71791437525871	38.0	34.0	40.0	23.0	41.0
49	34.693419470561565	38.0	34.0	40.0	23.0	41.0
50	34.611005657067395	38.0	34.0	40.0	23.0	41.0
51	33.726577868448544	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	14.0
10	20.0
11	16.0
12	13.0
13	21.0
14	24.0
15	50.0
16	84.0
17	209.0
18	548.0
19	1011.0
20	1946.0
21	3147.0
22	5228.0
23	8302.0
24	14357.0
25	24080.0
26	33837.0
27	35973.0
28	34087.0
29	33916.0
30	37461.0
31	44534.0
32	55540.0
33	72700.0
34	108586.0
35	140057.0
36	169434.0
37	250953.0
38	432008.0
39	520980.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.139839946385983	4.098220093430312	55.4722764275718	19.289663532611907
2	25.044596219423255	4.1251256578558335	50.34750556836774	20.482772554353183
3	18.005440245993732	3.913823349693493	56.70554668558926	21.375189718723515
4	16.555594977627976	4.194557782902647	56.043650089685215	23.206197149784163
5	16.702787140519977	4.968068121341139	56.0709005972444	22.258244140894487
6	19.475686436835986	5.50204009224765	58.4587448012142	16.563528669702166
7	79.6815682100408	1.5125756411014526	14.868872331618475	3.9369838172392724
8	81.9793822561252	1.6904677428892436	12.556373563558237	3.7737764374273155
9	76.94981570181145	4.264729071807305	14.396546626456153	4.388908599925098
10	33.312636745313704	28.894555811799027	23.35289653677094	14.439910906116335
11	21.101846924092797	24.61869000453354	34.223927226854315	20.055535844519344
12	18.904805550627795	22.10444878087241	37.9834624406205	21.007283227879288
13	20.12831884572172	22.21931484438137	39.00976681844164	18.642599491455268
14	18.502109080874384	24.299174107582836	37.61964598978969	19.5790708217531
15	17.45215145960223	25.89926674945302	37.69819446908324	18.950387321861513
16	18.84424339187511	25.18897955965545	36.012260264522105	19.954516783947334
17	19.372794827824098	25.633709814124927	34.19534622435101	20.798149133699958
18	19.326671003094635	24.62238582382276	36.07035854374864	19.980584629333965
19	19.92174718624958	26.894821910787854	33.84010407427118	19.343326828691385
20	21.11692586679282	26.951934638203927	33.95102793053831	17.98011156446494
21	21.384108962608163	26.495919815504703	34.299666883488065	17.82030433839907
22	20.46577178562277	24.092799558472787	34.1027043541679	21.33872430173654
23	18.864890702304223	25.646226322117755	33.53719472532671	21.951688250251316
24	19.15981708158398	25.522441014724144	34.46622513945558	20.851516764236298
25	18.92466441960854	26.907141308418588	32.4290895472375	21.739104724735377
26	19.524027753138984	27.435002858100248	32.66261604872568	20.378353340035087
27	19.772288254193523	26.292649754597598	33.78747560759269	20.147586383616186
28	18.593716121656513	25.238602093312046	35.09037510101906	21.077306684012378
29	19.669741588315297	23.595933613230045	34.94732225573098	21.78700254272367
30	20.850038436520606	24.98841976622711	33.95851812429779	20.203023672954487
31	19.723454161985295	26.863136420081602	32.44914552658033	20.96426389135277
32	19.287988094534132	27.332801135355684	32.23197918514577	21.14723158496442
33	19.979451244751935	26.51188575483413	31.5603749039087	21.948288096505234
34	18.704196479608935	26.663315790511106	31.600585417775413	23.031902312104545
35	19.859903810143297	26.36962135099442	30.623558630477206	23.146916208385075
36	20.90434234127688	27.51384700293694	29.839453610076283	21.74235704570989
37	18.84286361934047	27.388731200599214	32.3766581909211	21.391746989139218
38	17.75565213963298	28.34806733289969	32.616196558453076	21.280083969014253
39	18.213145290047898	27.350590345534464	31.515335186170734	22.9209291782469
40	19.372055663966254	27.599787120808937	29.94574537283425	23.082411842390556
41	18.948416218240595	27.68690990085349	29.133798513787866	24.23087536711805
42	20.36716732698638	26.645674413103897	29.568968915695898	23.418189344213825
43	20.243135631640154	25.77814243194765	31.269834230185477	22.708887706226715
44	19.771154869611497	25.723000808152484	30.758529950919524	23.7473143713165
45	20.962933396408648	25.07623243253898	29.972552382078728	23.988281788973648
46	21.260668598348214	26.09149863008298	29.956734275520862	22.69109849604794
47	19.313415331243963	26.04660674511659	31.785327104645887	22.854650818993555
48	18.883616186702934	25.53865334200619	32.34512053298642	23.232609938304456
49	19.65594386296888	24.646876786312657	32.48373839512743	23.213440955591036
50	19.183421047444465	24.340173062897914	31.653509549997043	24.822896339660577
51	18.412325310941597	24.235655293398775	30.3792403366645	26.972779058995133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	308.0
1	890.5
2	1473.0
3	19209.5
4	36946.0
5	25281.0
6	13616.0
7	13553.0
8	13490.0
9	14005.5
10	14521.0
11	14826.5
12	15132.0
13	15157.0
14	15182.0
15	14674.5
16	14167.0
17	13726.0
18	13285.0
19	13030.5
20	12776.0
21	12777.5
22	12779.0
23	13600.0
24	14421.0
25	15948.0
26	20095.5
27	22716.0
28	26778.5
29	30841.0
30	35428.5
31	40016.0
32	44885.5
33	49755.0
34	57985.5
35	66216.0
36	72269.5
37	78323.0
38	83818.0
39	89313.0
40	97217.0
41	105121.0
42	116984.0
43	128847.0
44	142633.5
45	156420.0
46	178085.0
47	199750.0
48	213080.0
49	226410.0
50	210160.5
51	193911.0
52	165683.0
53	137455.0
54	115678.5
55	93902.0
56	80155.0
57	66408.0
58	58702.5
59	50997.0
60	42961.0
61	34925.0
62	29292.0
63	23659.0
64	20174.5
65	16690.0
66	12798.5
67	8907.0
68	7222.5
69	5538.0
70	4556.0
71	3574.0
72	2631.0
73	1688.0
74	1484.0
75	932.0
76	584.0
77	406.5
78	229.0
79	142.5
80	56.0
81	75.5
82	95.0
83	77.0
84	59.0
85	48.0
86	37.0
87	21.5
88	6.0
89	10.5
90	15.0
91	10.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2029320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.92967314597043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.57366683142908	26.048338077183146
2	10.943405025992396	7.64499121323769
3	4.061385577852142	4.255886122624007
4	2.003244483195124	2.798907001178966
5	1.2193277658809798	2.129536016001449
6	0.833882006768292	1.747633556323341
7	0.6210901071384842	1.5186132105580108
8	0.4746828772865288	1.3264414201285795
9	0.4105688965312812	1.2906933623765466
>10	4.4061063024350515	34.58406599950604
>50	0.3854169522985814	8.488330926162396
>100	0.06107321212082776	3.7419003447991734
>500	0.003003469360326474	0.6948052876954457
>1k	0.002717424659343	2.0345585380129942
>5k	2.860447009834737E-4	0.5445838683612347
>10k+	1.4302235049173684E-4	1.1507150558510852
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23034	1.135060020105257	No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGCT	5508	0.2714209686003193	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	5393	0.2657540456901819	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	4118	0.20292511777344138	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	3857	0.19006366664695562	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCC	3267	0.1609898882384247	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT	3221	0.15872311907436976	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT	3058	0.15069087181913154	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTC	3006	0.148128437111939	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG	2329	0.11476750832791281	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG	2302	0.1134370133837936	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.927759052293379E-5	0.0	0.0	0.2958626534996945	0.0
2	4.927759052293379E-5	0.0	0.0	1.0418268188358661	0.0
3	4.927759052293379E-5	0.0	0.0	1.6162556915617055	0.0
4	4.927759052293379E-5	0.0	0.0	2.3740464786233813	0.0
5	4.927759052293379E-5	0.0	0.0	3.635651351191532	0.0
6	4.927759052293379E-5	0.0	0.0	4.759180415114423	0.0
7	4.927759052293379E-5	0.0	0.0	5.7035361598959256	0.0
8	4.927759052293379E-5	0.0	0.0	6.974848717797094	0.0
9	4.927759052293379E-5	0.0	0.0	7.652317032306389	0.0
10	4.927759052293379E-5	0.0	0.0	8.76781384897404	0.0
11	4.927759052293379E-5	0.0	0.0	10.680671357893285	0.0
12	4.927759052293379E-5	0.0	0.0	12.23345751286145	0.0
13	4.927759052293379E-5	0.0	0.0	12.766788875091164	0.0
14	9.855518104586758E-5	0.0	0.0	12.961336802475707	0.0
15	9.855518104586758E-5	0.0	0.0	13.204570989296908	6.898862673210731E-4
16	9.855518104586758E-5	0.0	0.0	13.7765359824966	6.898862673210731E-4
17	9.855518104586758E-5	0.0	0.0	14.699800918534287	6.898862673210731E-4
18	9.855518104586758E-5	0.0	0.0	15.69018193286421	6.898862673210731E-4
19	9.855518104586758E-5	0.0	0.0	16.424960085151678	6.898862673210731E-4
20	9.855518104586758E-5	0.0	0.0	17.11765517513256	6.898862673210731E-4
21	9.855518104586758E-5	0.0	0.0	18.166282301460587	6.898862673210731E-4
22	9.855518104586758E-5	0.0	0.0	19.296660950466165	6.898862673210731E-4
23	9.855518104586758E-5	0.0	0.0	20.468531330692056	6.898862673210731E-4
24	9.855518104586758E-5	0.0	0.0	21.32275836240711	6.898862673210731E-4
25	9.855518104586758E-5	0.0	0.0	22.070250133049495	6.898862673210731E-4
26	9.855518104586758E-5	0.0	0.0	22.728697297616936	6.898862673210731E-4
27	9.855518104586758E-5	0.0	0.0	23.382758756627837	6.898862673210731E-4
28	1.4783277156880137E-4	0.0	0.0	24.0829440403682	6.898862673210731E-4
29	1.4783277156880137E-4	0.0	0.0	24.82885892811385	6.898862673210731E-4
30	1.9711036209173516E-4	0.0	0.0	25.60434037017326	6.898862673210731E-4
31	3.4494313366053653E-4	0.0	0.0	26.382088581396722	6.898862673210731E-4
32	3.9422072418347033E-4	0.0	0.0	27.088384286361933	6.898862673210731E-4
33	3.9422072418347033E-4	0.0	0.0	27.80552106124219	6.898862673210731E-4
34	4.434983147064041E-4	0.0	0.0	28.48983896083417	6.898862673210731E-4
35	4.434983147064041E-4	0.0	0.0	29.208306230658547	6.898862673210731E-4
36	4.434983147064041E-4	0.0	0.0	29.89691128062602	6.898862673210731E-4
37	4.434983147064041E-4	0.0	0.0	30.580194350817024	6.898862673210731E-4
38	4.434983147064041E-4	0.0	0.0	31.27402282537993	6.898862673210731E-4
39	4.434983147064041E-4	0.0	0.0	31.955039126406874	6.898862673210731E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	40	6.8193913E-9	45.0	1
GCGTAAT	20	7.0345646E-4	45.0	9
CGTTTTT	16245	0.0	44.030468	1
GCGCGAC	140	0.0	41.785713	9
CTAGCGG	110	0.0	40.909092	2
GCTACGA	195	0.0	40.384617	10
GATACGA	35	6.250655E-6	38.571426	9
TTTTGCG	1750	0.0	38.314285	1
TTGGGAC	5250	0.0	38.271427	5
GGCGATC	665	0.0	38.23308	8
GGCACCG	660	0.0	38.18182	8
TCTTGCG	640	0.0	37.96875	1
TTTGGGC	5545	0.0	37.858433	4
CGCGACC	155	0.0	37.741936	10
GACCGAT	865	0.0	37.716763	9
CGGGATC	910	0.0	37.58242	6
AGCGTCG	30	1.1402301E-4	37.499996	17
CCGGTCG	30	1.1402301E-4	37.499996	16
CGCACCG	30	1.1402301E-4	37.499996	22
CGTTTGG	1295	0.0	37.355213	2
>>END_MODULE