FastQCFastQC Report
Sat 14 Jan 2017
SRR2934426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082706
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC193770.9303761548677538No Hit
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT161390.774905339495829No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152210.7308280669475192No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCC131000.628989401288516No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC101370.4867225618978387No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC86800.41676549642628385No Hit
TCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG62260.2989380162154428No Hit
TTCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT60320.2896232113413991No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC46250.22206686877552567No Hit
TGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG41860.20098852166364334No Hit
TTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG41570.19959610237834818No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG33810.1623368828821735No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG30660.14721232857638092No Hit
TTTCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC30270.14533976470994947No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG29710.14265095505558634No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTC28500.13684120562383745No Hit
TTGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT28240.13559282971288314No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC26580.12762242966602103No Hit
TTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT24340.11686719104856853No Hit
CGTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT22690.10894480545982005No Hit
TGCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT22620.10860870425302468No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGC207.0346065E-445.00000441
ACCGGCG406.8193913E-945.00000420
TCCGCTA253.8919003E-545.00000415
CGTTCGC207.0346065E-445.00000416
ACGACTA207.0346065E-445.00000412
CCGTGCG351.2125565E-745.01
CGTTTTT70350.043.368871
CGAACGA650.041.5384622
TATCGCA501.0822987E-940.50000428
CTATGCG451.929584E-840.0000041
TCGATCA2150.039.7674417
TGGGCGC3000.039.756
TTGTGCG4250.039.7058831
TCGTTAC800.039.3750041
TGGGCGA22500.038.96
TTTGGGA144850.038.864344
GGCCGAT5750.038.7391328
TTGGGCG17550.038.717955
TCGCACG356.250735E-638.5714261
TACGGGT356.250735E-638.5714264
Produced by FastQC (version 0.11.5)