SRR2934424.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934424.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56879
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCT	1256	2.2081963466305665	RNA PCR Primer, Index 17 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	671	1.1796972520613935	Illumina PCR Primer Index 9 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT	479	0.8421385748694598	RNA PCR Primer, Index 17 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	472	0.8298317480968372	Illumina PCR Primer Index 9 (95% over 23bp)
CGGTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	425	0.7472001969092283	RNA PCR Primer, Index 17 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	353	0.6206156929622532	RNA PCR Primer, Index 17 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	185	0.32525185041931115	RNA PCR Primer, Index 17 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	130	0.22855535434870514	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCT	118	0.2074579370242093	Illumina PCR Primer Index 9 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	113	0.198667346472336	Illumina PCR Primer Index 9 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.18811863781008809	No Hit
GCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	94	0.16526310237521757	Illumina PCR Primer Index 9 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	86	0.15119815749222032	Illumina PCR Primer Index 9 (95% over 22bp)
CGGTGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTT	82	0.14416568505072172	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	79	0.13889133071959775	No Hit
GTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	68	0.11955203150547652	RNA PCR Primer, Index 17 (95% over 23bp)
TTGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCT	66	0.11603579528472724	RNA PCR Primer, Index 17 (95% over 22bp)
GTTCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	65	0.11427767717435257	RNA PCR Primer, Index 17 (95% over 21bp)
CGGTGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCT	62	0.10900332284322861	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCG	58	0.10197085040173	No Hit
TGCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCT	57	0.10021273229135533	RNA PCR Primer, Index 17 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCTG	40	6.6211214E-9	45.000004	1
GGGTCCT	40	6.6211214E-9	45.000004	7
ATTAGTC	20	6.97679E-4	45.000004	31
TTATTGG	20	6.97679E-4	45.000004	2
TAGTCAG	20	6.97679E-4	45.000004	33
TCTGTGG	40	6.6211214E-9	45.000004	2
AGCGGAG	20	6.97679E-4	45.000004	38
CGGGAGT	20	6.97679E-4	45.000004	6
TATTTGG	20	6.97679E-4	45.000004	2
AGCATAT	20	6.97679E-4	45.000004	26
GGAGGGA	20	6.97679E-4	45.000004	8
TATTAGT	20	6.97679E-4	45.000004	30
GGGGTGT	20	6.97679E-4	45.000004	7
CAGCGGA	20	6.97679E-4	45.000004	37
CTTTGCG	20	6.97679E-4	45.000004	1
CATATTA	20	6.97679E-4	45.000004	28
CTCGTGG	20	6.97679E-4	45.000004	2
TTAGTCA	20	6.97679E-4	45.000004	32
GCATATT	20	6.97679E-4	45.000004	27
GTGTTGG	25	3.844055E-5	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content