##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934424.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56879
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.899242251094428	33.0	31.0	34.0	30.0	34.0
2	32.097030538511575	33.0	31.0	34.0	30.0	34.0
3	32.18704618576276	34.0	31.0	34.0	30.0	34.0
4	35.70613055785088	37.0	35.0	37.0	33.0	37.0
5	35.68441779918775	37.0	35.0	37.0	35.0	37.0
6	35.34452082490902	37.0	35.0	37.0	33.0	37.0
7	35.60036217233074	37.0	35.0	37.0	35.0	37.0
8	35.68436505564444	37.0	35.0	37.0	35.0	37.0
9	37.38446526837673	39.0	37.0	39.0	35.0	39.0
10	36.99119182826702	39.0	37.0	39.0	33.0	39.0
11	36.95910617275268	39.0	37.0	39.0	33.0	39.0
12	36.81836881801719	39.0	35.0	39.0	33.0	39.0
13	36.625731816663446	39.0	35.0	39.0	33.0	39.0
14	37.6217760509151	39.0	36.0	41.0	33.0	41.0
15	37.67033527312365	39.0	36.0	41.0	33.0	41.0
16	37.73420770407356	39.0	36.0	41.0	33.0	41.0
17	37.698342094621914	39.0	36.0	41.0	33.0	41.0
18	37.75804778565024	39.0	36.0	41.0	33.0	41.0
19	37.79488036006259	39.0	36.0	41.0	33.0	41.0
20	37.75896200706764	39.0	36.0	41.0	33.0	41.0
21	37.57975702807715	39.0	36.0	41.0	33.0	41.0
22	37.659979957453544	39.0	36.0	41.0	33.0	41.0
23	37.60192689744897	39.0	36.0	41.0	33.0	41.0
24	37.522846744844315	39.0	36.0	41.0	33.0	41.0
25	37.448742066492024	39.0	36.0	41.0	32.0	41.0
26	37.37620211325797	39.0	36.0	41.0	32.0	41.0
27	37.2435345206491	39.0	35.0	41.0	32.0	41.0
28	37.12878215158494	39.0	35.0	41.0	32.0	41.0
29	36.95835018196522	39.0	35.0	40.0	31.0	41.0
30	36.904516605425556	39.0	35.0	40.0	31.0	41.0
31	36.62051020587563	39.0	35.0	40.0	31.0	41.0
32	36.5776472863447	39.0	35.0	40.0	30.0	41.0
33	36.49348617240106	38.0	35.0	40.0	30.0	41.0
34	36.530863763427625	39.0	35.0	40.0	30.0	41.0
35	36.49051495279453	39.0	35.0	40.0	30.0	41.0
36	36.38263682554194	38.0	35.0	40.0	30.0	41.0
37	36.23458569946729	38.0	35.0	40.0	30.0	41.0
38	36.046062694491816	38.0	35.0	40.0	29.0	41.0
39	35.904305631252306	38.0	35.0	40.0	29.0	41.0
40	35.78222190966789	38.0	34.0	40.0	28.0	41.0
41	35.80597408533905	38.0	35.0	40.0	28.0	41.0
42	35.83055257652209	38.0	35.0	40.0	28.0	41.0
43	35.763849575414476	38.0	34.0	40.0	28.0	41.0
44	35.520315054765376	38.0	34.0	40.0	28.0	41.0
45	35.412612739323826	38.0	34.0	40.0	27.0	41.0
46	35.194359957101916	38.0	34.0	40.0	26.0	41.0
47	34.90866576416604	37.0	33.0	40.0	26.0	41.0
48	34.9000334042441	37.0	34.0	40.0	26.0	41.0
49	34.93581110779022	37.0	34.0	40.0	26.0	41.0
50	34.76896569911567	36.0	34.0	39.0	26.0	41.0
51	33.69079976792841	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	11.0
11	2.0
12	1.0
13	1.0
14	5.0
15	7.0
16	4.0
17	5.0
18	19.0
19	49.0
20	52.0
21	83.0
22	155.0
23	190.0
24	247.0
25	357.0
26	395.0
27	553.0
28	749.0
29	916.0
30	1161.0
31	1426.0
32	1970.0
33	2601.0
34	4132.0
35	5296.0
36	5664.0
37	8098.0
38	12105.0
39	10614.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.80491921447283	4.729337716907822	52.086007137959534	19.379735930659823
2	22.131190773396156	5.131946764183618	49.63870672831801	23.098155734102217
3	23.702948364071098	4.713514653914451	48.910845830622904	22.67269115139155
4	19.494013607834173	4.89108458306229	47.968494523462084	27.646407285641452
5	17.015067072205913	5.332372228766329	51.753722815098726	25.898837883929044
6	22.570720300989823	5.926616150072962	52.20555916946501	19.297104379472213
7	77.5593804391779	2.1062254962288365	14.256579757028076	6.077814307565182
8	76.53439758082948	3.159338244343255	10.112695370875015	10.19356880395225
9	69.2839184936444	7.491341268306405	14.951036410626065	8.273703827423127
10	32.3528894671144	31.55294572689393	19.10722762355175	16.986937182439917
11	23.64317234831836	27.192812813164792	29.522319309411206	19.641695529105647
12	22.929376395506253	19.41314017475694	38.65925912902829	18.99822430070852
13	24.843967017704248	27.23500764781378	31.732273774152148	16.188751560329823
14	17.269994198210238	30.872554018178946	32.134882821427944	19.72256896218288
15	13.280824205770145	28.316250285694196	37.86634786124932	20.536577647286343
16	15.735157087853164	28.710068742418116	27.945287364405143	27.60948680532358
17	16.60542555248862	26.361222946957575	26.531760403663917	30.50159109688989
18	19.28479755269959	23.70646460029185	30.772341285887585	26.236396561120976
19	21.695177482023244	25.970920726454406	29.578579088943197	22.755322702579157
20	21.87098929306071	26.231122206789852	31.96786160094235	19.93002689920709
21	21.34355385994831	26.050036041421265	31.64612598674379	20.960284111886637
22	19.397317111763567	24.0703950491394	30.3205049315213	26.21178290757573
23	20.302747938606515	25.364369978375144	28.861266899910337	25.471615183108
24	21.93955589936532	26.35067423829533	26.619666309182648	25.090103553156702
25	18.61671267075722	27.097874435204556	27.377415214754127	26.907997679284097
26	22.354471773413735	26.619666309182648	25.34327256105065	25.68258935635296
27	21.016543891418628	27.210393994268532	26.028938624096764	25.744123490216076
28	21.774292796990103	24.89495244290511	28.325040876246067	25.005713883858714
29	21.38047434026618	27.42840063995499	24.36751700979272	26.82360800998611
30	26.31375375797746	26.072891576856133	23.67657659241548	23.93677807275093
31	25.178009458675433	33.79454631762162	19.907171363772218	21.12027285993073
32	25.000439529527597	30.506865451221017	21.593206631621513	22.899488387629884
33	23.055960899453225	32.78890275848732	22.257775277343132	21.89736106471633
34	20.97259093865926	26.624940663513776	29.175970041667398	23.226498356159567
35	20.93918669456214	27.45301429350024	25.28349654529791	26.32430246663971
36	26.846463545420978	25.279980309077168	27.263137537579773	20.610418607922078
37	22.767629529351783	27.036340301341443	26.371771655619824	23.82425851368695
38	23.92974560030943	30.540269695318134	23.581638214455246	21.948346489917192
39	24.794739710613758	27.18578034072329	27.29478366356652	20.724696285096435
40	25.777527734313193	28.73995675029449	25.30811019884316	20.174405316549166
41	19.845637229909105	26.89217461629072	26.021906151655266	27.240282002144905
42	25.98146943511665	23.943810545192427	25.32393326183653	24.750786757854392
43	24.39916313577946	24.624202253907416	23.973698553068797	27.002936057244327
44	24.049297631814905	23.76448249793421	24.784191001951513	27.40202886829937
45	25.55073049807486	25.003955765748344	22.407215316724976	27.038098419451817
46	25.974436962675153	24.597830482251798	29.443203994444346	19.984528560628704
47	18.535839237679987	27.512790309252978	31.162643506390758	22.788726946676277
48	18.88394662353417	25.819722568962185	32.15422212064206	23.142108686861583
49	20.842490198491532	23.773273088486086	34.16375112080029	21.220485592222087
50	21.99933191511806	21.107966033158107	29.314861372386996	27.57784067933684
51	19.981012324407953	22.270082104115755	26.54055099421579	31.2083545772605
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	22.0
2	37.0
3	95.0
4	153.0
5	106.5
6	60.0
7	76.0
8	92.0
9	111.0
10	130.0
11	163.0
12	196.0
13	215.0
14	234.0
15	246.5
16	259.0
17	228.0
18	197.0
19	197.5
20	198.0
21	216.0
22	234.0
23	235.5
24	237.0
25	272.5
26	352.5
27	397.0
28	431.0
29	465.0
30	562.0
31	659.0
32	731.0
33	803.0
34	983.5
35	1164.0
36	1274.5
37	1385.0
38	1393.5
39	1402.0
40	1777.0
41	2152.0
42	2797.0
43	3442.0
44	4085.5
45	4729.0
46	5551.5
47	6374.0
48	7136.5
49	7899.0
50	7783.0
51	7667.0
52	5715.0
53	3763.0
54	3342.0
55	2921.0
56	2721.5
57	2522.0
58	2254.0
59	1986.0
60	1669.0
61	1352.0
62	1224.0
63	1096.0
64	948.0
65	800.0
66	672.5
67	545.0
68	448.0
69	351.0
70	290.0
71	229.0
72	224.0
73	219.0
74	172.0
75	82.0
76	39.0
77	35.0
78	31.0
79	21.5
80	12.0
81	9.0
82	6.0
83	4.0
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	56879.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.793509027936494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.67547968940512	43.10905606638654
2	8.669704164794968	9.500870268464636
3	3.8760187383687352	6.37142003199775
4	2.595777449785022	5.689270205172384
5	1.8160816274144902	4.975474252360273
6	1.334787909901816	4.388262803495139
7	0.9144580632740807	3.5074456301974366
8	0.6385163319001477	2.7989240317164508
9	0.35294872617596096	1.7405369292709083
>10	1.0492203041776293	8.794106788094023
>50	0.04171212218443175	1.548902055240071
>100	0.028877623050760446	4.187837338912428
>500	0.0032086247834178273	1.1796972520613935
>1k	0.0032086247834178273	2.2081963466305665
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCT	1256	2.2081963466305665	RNA PCR Primer, Index 17 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	671	1.1796972520613935	Illumina PCR Primer Index 9 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT	479	0.8421385748694598	RNA PCR Primer, Index 17 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	472	0.8298317480968372	Illumina PCR Primer Index 9 (95% over 23bp)
CGGTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	425	0.7472001969092283	RNA PCR Primer, Index 17 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	353	0.6206156929622532	RNA PCR Primer, Index 17 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	185	0.32525185041931115	RNA PCR Primer, Index 17 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	130	0.22855535434870514	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCT	118	0.2074579370242093	Illumina PCR Primer Index 9 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	113	0.198667346472336	Illumina PCR Primer Index 9 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.18811863781008809	No Hit
GCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	94	0.16526310237521757	Illumina PCR Primer Index 9 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	86	0.15119815749222032	Illumina PCR Primer Index 9 (95% over 22bp)
CGGTGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTT	82	0.14416568505072172	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	79	0.13889133071959775	No Hit
GTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	68	0.11955203150547652	RNA PCR Primer, Index 17 (95% over 23bp)
TTGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCT	66	0.11603579528472724	RNA PCR Primer, Index 17 (95% over 22bp)
GTTCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	65	0.11427767717435257	RNA PCR Primer, Index 17 (95% over 21bp)
CGGTGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCT	62	0.10900332284322861	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCG	58	0.10197085040173	No Hit
TGCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCT	57	0.10021273229135533	RNA PCR Primer, Index 17 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0017581181103746549	0.0	0.0	0.9036727087325727	0.0
2	0.0017581181103746549	0.0	0.0	3.8397299530582463	0.0
3	0.0017581181103746549	0.0	0.0	5.314791047662582	0.0
4	0.0017581181103746549	0.0	0.0	9.425271189718526	0.0
5	0.0017581181103746549	0.0	0.0	12.725258882891753	0.0
6	0.0017581181103746549	0.0	0.0	14.028024402679373	0.0
7	0.0017581181103746549	0.0	0.0	15.091685859456039	0.0
8	0.0017581181103746549	0.0	0.0	16.681024631234727	0.0
9	0.0017581181103746549	0.0	0.0	17.538986269097556	0.0
10	0.0017581181103746549	0.0	0.0	19.27424884403734	0.0
11	0.0017581181103746549	0.0	0.0	22.247226568680883	0.0
12	0.0017581181103746549	0.0	0.0	25.14284709646794	0.0
13	0.0017581181103746549	0.0	0.0	26.02190615165527	0.0
14	0.0017581181103746549	0.0	0.0	26.27859139576997	0.0
15	0.0017581181103746549	0.0	0.0	26.700539742259885	0.0
16	0.0017581181103746549	0.0	0.0	28.00857961637863	0.0
17	0.0017581181103746549	0.0	0.0	30.367974120501415	0.0
18	0.0017581181103746549	0.0	0.0	33.7752070184075	0.0
19	0.0017581181103746549	0.0	0.0	34.82128729408041	0.0
20	0.0017581181103746549	0.0	0.0	36.22250742804901	0.0
21	0.0017581181103746549	0.0	0.0	37.2228766328522	0.0
22	0.0017581181103746549	0.0	0.0	37.91909140456056	0.0
23	0.0017581181103746549	0.0	0.0	38.54322333374356	0.0
24	0.0017581181103746549	0.0	0.0	38.98626909755797	0.0
25	0.0017581181103746549	0.0	0.0	39.37832943617152	0.0
26	0.0017581181103746549	0.0	0.0	39.77038977478507	0.0
27	0.0017581181103746549	0.0	0.0	40.15190140473637	0.0
28	0.0017581181103746549	0.0	0.0	40.57912410555741	0.0
29	0.0017581181103746549	0.0	0.0	41.05029975913782	0.0
30	0.0017581181103746549	0.0	0.0	41.53202412138047	0.0
31	0.0017581181103746549	0.0	0.0	41.90298704266953	0.0
32	0.0017581181103746549	0.0	0.0	42.23527136553034	0.0
33	0.0017581181103746549	0.0	0.0	42.59041122382602	0.0
34	0.0017581181103746549	0.0	0.0	42.9385186096802	0.0
35	0.0017581181103746549	0.0	0.0	43.32530459396262	0.0
36	0.0017581181103746549	0.0	0.0	43.65934703493381	0.0
37	0.0017581181103746549	0.0	0.0	43.965259586139	0.0
38	0.0017581181103746549	0.0	0.0	44.28347896411681	0.0
39	0.0017581181103746549	0.0	0.0	44.719492255489726	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCTG	40	6.6211214E-9	45.000004	1
GGGTCCT	40	6.6211214E-9	45.000004	7
ATTAGTC	20	6.97679E-4	45.000004	31
TTATTGG	20	6.97679E-4	45.000004	2
TAGTCAG	20	6.97679E-4	45.000004	33
TCTGTGG	40	6.6211214E-9	45.000004	2
AGCGGAG	20	6.97679E-4	45.000004	38
CGGGAGT	20	6.97679E-4	45.000004	6
TATTTGG	20	6.97679E-4	45.000004	2
AGCATAT	20	6.97679E-4	45.000004	26
GGAGGGA	20	6.97679E-4	45.000004	8
TATTAGT	20	6.97679E-4	45.000004	30
GGGGTGT	20	6.97679E-4	45.000004	7
CAGCGGA	20	6.97679E-4	45.000004	37
CTTTGCG	20	6.97679E-4	45.000004	1
CATATTA	20	6.97679E-4	45.000004	28
CTCGTGG	20	6.97679E-4	45.000004	2
TTAGTCA	20	6.97679E-4	45.000004	32
GCATATT	20	6.97679E-4	45.000004	27
GTGTTGG	25	3.844055E-5	45.0	2
>>END_MODULE