##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934423.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	641887
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.155158150889484	33.0	31.0	34.0	30.0	34.0
2	32.352160115409724	34.0	31.0	34.0	30.0	34.0
3	32.41278449322077	34.0	31.0	34.0	30.0	34.0
4	35.92594335139986	37.0	35.0	37.0	35.0	37.0
5	35.8839702315205	37.0	35.0	37.0	35.0	37.0
6	35.55818547501352	37.0	35.0	37.0	33.0	37.0
7	35.884716468786564	37.0	35.0	37.0	35.0	37.0
8	35.96936376651965	37.0	35.0	37.0	35.0	37.0
9	37.73461995647209	39.0	38.0	39.0	35.0	39.0
10	37.27006466870337	39.0	37.0	39.0	34.0	39.0
11	37.128647254111705	39.0	37.0	39.0	34.0	39.0
12	36.66477277153144	39.0	35.0	39.0	33.0	39.0
13	36.41201021363573	39.0	35.0	39.0	32.0	39.0
14	36.993403823414404	39.0	35.0	41.0	31.0	41.0
15	37.2164493127295	39.0	35.0	41.0	31.0	41.0
16	37.46362989124254	39.0	35.0	41.0	33.0	41.0
17	37.473463397763155	39.0	35.0	41.0	33.0	41.0
18	37.488963010623365	39.0	35.0	41.0	33.0	41.0
19	37.52377443381779	39.0	35.0	41.0	33.0	41.0
20	37.409746575331795	39.0	35.0	41.0	32.0	41.0
21	37.279867017091796	39.0	35.0	41.0	32.0	41.0
22	37.46362521752271	39.0	35.0	41.0	33.0	41.0
23	37.45446161084428	39.0	35.0	41.0	33.0	41.0
24	37.400618800505384	39.0	35.0	41.0	33.0	41.0
25	37.336534312114125	39.0	35.0	41.0	33.0	41.0
26	37.25831961077261	39.0	35.0	41.0	33.0	41.0
27	37.1967402362098	39.0	35.0	41.0	33.0	41.0
28	37.11659996229866	39.0	35.0	41.0	32.0	41.0
29	37.02115792966675	39.0	35.0	40.0	32.0	41.0
30	36.98388501402895	39.0	35.0	40.0	32.0	41.0
31	36.82214626561996	39.0	35.0	40.0	31.0	41.0
32	36.737706169465966	39.0	35.0	40.0	31.0	41.0
33	36.66859431644511	39.0	35.0	40.0	31.0	41.0
34	36.48442482866922	38.0	35.0	40.0	31.0	41.0
35	36.33751579327826	38.0	35.0	40.0	30.0	41.0
36	35.86584866183612	38.0	35.0	40.0	29.0	41.0
37	35.637574837938764	38.0	34.0	40.0	29.0	41.0
38	35.39432641570868	38.0	34.0	40.0	27.0	41.0
39	35.002456818723545	38.0	34.0	40.0	24.0	41.0
40	34.61619412762683	38.0	34.0	40.0	23.0	41.0
41	34.40563525978872	37.0	34.0	40.0	21.0	41.0
42	34.257129370122776	37.0	33.0	40.0	21.0	41.0
43	34.01018715132103	37.0	33.0	40.0	18.0	41.0
44	33.650879360385865	37.0	33.0	40.0	15.0	41.0
45	33.20229105746027	36.0	33.0	40.0	15.0	41.0
46	32.61306117743466	35.0	32.0	40.0	12.0	41.0
47	32.35329894514143	35.0	31.0	40.0	12.0	41.0
48	32.258070345715055	35.0	31.0	40.0	12.0	41.0
49	32.16974950419622	35.0	31.0	40.0	12.0	41.0
50	31.877707446949387	35.0	30.0	40.0	11.0	41.0
51	30.752454871340284	35.0	27.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	14.0
10	15.0
11	5.0
12	16.0
13	10.0
14	12.0
15	28.0
16	38.0
17	83.0
18	175.0
19	288.0
20	503.0
21	805.0
22	1272.0
23	2106.0
24	3234.0
25	4863.0
26	6637.0
27	8941.0
28	11590.0
29	15480.0
30	20214.0
31	25405.0
32	31637.0
33	41498.0
34	64827.0
35	60025.0
36	49076.0
37	64850.0
38	102315.0
39	125888.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.757764217066242	4.728869723175574	55.07776290842469	16.43560315133349
2	21.131601045043755	4.2322090959935315	52.95823096588652	21.677958893076195
3	21.55613059619528	4.772335317587053	50.82498944518272	22.84654464103495
4	20.16195997114757	4.516527052269324	49.9034876855272	25.4180252910559
5	19.098065547362697	5.688072822786565	50.558120042935904	24.655741586914832
6	22.126791787339517	5.803669493228559	53.15390403606865	18.915634683363272
7	82.44472936825329	2.488755809044271	10.380175949972504	4.6863388727299355
8	83.16728645384623	2.8308721005410606	8.614133017182152	5.38770842843055
9	77.27746472509959	5.407805423696071	11.37817092416578	5.9365589270385595
10	44.14826908786126	24.08336669849989	18.351049327996986	13.417314885641865
11	35.295464778068414	22.405189698498333	24.270471282328508	18.02887424110474
12	33.03369596206186	19.830281653935973	28.935155253183193	18.200867130818978
13	29.622192068074288	21.650383946083966	29.77876168235219	18.948662303489556
14	20.163985249740218	24.91778147867148	34.81407163566173	20.104161635926573
15	17.711061292719744	23.97306691053098	38.63842078122785	19.677451015521424
16	25.40119989967081	22.060424965765	32.423619733691446	20.11475540087274
17	25.698760062129317	22.059490221799162	28.697574495199312	23.54417522087221
18	26.654691557859874	21.735757228297192	30.718335158680578	20.891216055162356
19	26.96222232262065	24.981032487026532	28.40764807512849	19.649097115224333
20	26.3032278267047	28.555649203052873	27.92158121289261	17.219541757349813
21	24.15347249593776	26.246987398093435	31.456627101031803	18.142913004937007
22	21.734355112348435	24.155341983869434	32.612749596112714	21.497553307669417
23	21.525284045322618	24.900488715303474	31.738608197393	21.83561904198091
24	23.657746612721553	25.779615415174323	28.28005552379157	22.282582448312553
25	24.42376929272598	23.980233280935742	26.60359222106072	24.992405205277564
26	19.725278748440147	25.22297538351766	28.543341740836002	26.50840412720619
27	19.033724004380833	25.099589180027017	33.33951302955193	22.527173786040223
28	17.878068881282843	24.146461916193974	32.26533642214284	25.710132780380345
29	20.754120273506086	25.955658862073854	31.09020746642322	22.20001339799684
30	24.40382808812143	22.97725300559133	31.991768021474186	20.62715088481306
31	25.027769685318447	25.001441063614	30.266074869875855	19.704714381191703
32	27.855525972640045	24.198028624976047	27.774826410256008	20.1716189921279
33	26.866722647444174	23.198319953512065	28.15775985492774	21.77719754411602
34	21.91725334833078	22.823020251228023	33.171259115701055	22.088467284740148
35	21.539616786132136	25.3388836352816	30.38058100569103	22.74091857289523
36	22.625633483775182	25.792701830696057	29.891086748913747	21.69057793661501
37	22.082547239623175	27.44704285956874	29.64602803920316	20.824381861604923
38	24.310976854181497	25.65545025837881	27.04198714103884	22.991585746400848
39	23.99768183496472	22.09672418977172	28.75848864363977	25.14710533162379
40	26.912992473753167	21.332882579020918	29.357192153759147	22.39693279346676
41	22.3065742101024	22.065098685594194	27.761272622751353	27.86705448155205
42	21.18877621762086	21.661445083013053	30.503811418520705	26.645967280845383
43	20.930007929744644	21.439443391126474	30.730331039575503	26.900217639553382
44	22.369825218457454	21.67094831333241	29.72890867084082	26.230317797369317
45	21.381333474583535	21.13347053297543	27.646454905614227	29.838741086826808
46	21.585107659136266	21.586665565745996	30.196903816403818	26.631322958713916
47	19.595972499832527	23.61038625178575	32.84035975179432	23.953281496587405
48	20.370563666190467	23.958734169721463	31.749825124983055	23.920877039105015
49	23.627834805814732	20.400787054419236	32.66431630489478	23.307061834871245
50	21.178805615318584	21.37447868550072	29.855566478211898	27.591149220968802
51	19.13140474881093	21.81162728019106	28.277251291894057	30.779716679103952
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	481.5
2	811.0
3	1993.5
4	3176.0
5	2147.5
6	1119.0
7	1143.5
8	1168.0
9	1361.0
10	1554.0
11	1677.0
12	1800.0
13	1891.0
14	1982.0
15	1859.0
16	1736.0
17	1787.0
18	1838.0
19	1927.0
20	2016.0
21	1984.5
22	1953.0
23	2090.5
24	2228.0
25	3022.5
26	4566.0
27	5315.0
28	6120.5
29	6926.0
30	7999.0
31	9072.0
32	10003.0
33	10934.0
34	12952.0
35	14970.0
36	16325.0
37	17680.0
38	19498.0
39	21316.0
40	24468.0
41	27620.0
42	30380.0
43	33140.0
44	37339.0
45	41538.0
46	46383.5
47	51229.0
48	57789.5
49	64350.0
50	65328.5
51	66307.0
52	61854.0
53	57401.0
54	51228.0
55	45055.0
56	40733.5
57	36412.0
58	34018.0
59	31624.0
60	28132.0
61	24640.0
62	21512.5
63	18385.0
64	15313.5
65	12242.0
66	9766.0
67	7290.0
68	6139.0
69	4988.0
70	3846.0
71	2704.0
72	2567.5
73	2431.0
74	1959.0
75	1121.0
76	755.0
77	545.5
78	336.0
79	239.5
80	143.0
81	118.0
82	93.0
83	84.5
84	76.0
85	71.5
86	67.0
87	36.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	641887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.966751450046445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.61035449350962	28.320359330615187
2	10.217917344883212	7.5544642165083244
3	3.9187786923378756	4.345935537221768
4	1.9580783388319616	2.895351810852838
5	1.124464796337698	2.0783905320271385
6	0.7256975852218852	1.6096009356477796
7	0.48268223651924413	1.2490235986731597
8	0.37109387372336117	1.097450799565313
9	0.2993408251561833	0.9959092094160396
>10	3.726487557846615	34.33077947566211
>50	0.5162418575989275	11.859119147088748
>100	0.04716283636274959	3.024976167976514
>500	0.001274671253047286	0.33706832423685545
>1k	4.2489041768242877E-4	0.301570914508277
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1920	0.29911806906823163	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	932	0.14519689602687078	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5579066097303731E-4	0.0	0.0	0.4066136251396274	0.0
2	1.5579066097303731E-4	0.0	0.0	1.7862957187168458	0.0
3	1.5579066097303731E-4	0.0	0.0	2.5535647240090547	0.0
4	1.5579066097303731E-4	0.0	0.0	3.457150557652671	0.0
5	1.5579066097303731E-4	0.0	0.0	4.829510490164156	0.0
6	1.5579066097303731E-4	0.0	0.0	5.686514916176835	0.0
7	1.5579066097303731E-4	0.0	0.0	6.469362987566347	0.0
8	1.5579066097303731E-4	0.0	0.0	7.598377907637949	0.0
9	1.5579066097303731E-4	0.0	0.0	8.188668722064786	0.0
10	1.5579066097303731E-4	0.0	0.0	9.268609583929882	0.0
11	1.5579066097303731E-4	0.0	0.0	11.501868708978371	0.0
12	1.5579066097303731E-4	0.0	0.0	13.40220319152748	0.0
13	3.1158132194607463E-4	0.0	0.0	14.001529864290756	0.0
14	3.1158132194607463E-4	0.0	0.0	14.256403385642644	0.0
15	3.1158132194607463E-4	0.0	0.0	14.614566115219658	0.0
16	3.1158132194607463E-4	0.0	0.0	15.42311964566972	0.0
17	3.1158132194607463E-4	0.0	0.0	16.532349151797746	0.0
18	3.1158132194607463E-4	0.0	0.0	17.766834349348095	0.0
19	3.1158132194607463E-4	0.0	0.0	18.541581306367007	0.0
20	3.1158132194607463E-4	0.0	0.0	19.443141861417974	0.0
21	3.1158132194607463E-4	0.0	0.0	20.62621614084722	0.0
22	6.231626438921493E-4	0.0	0.0	21.823311579764038	0.0
23	6.231626438921493E-4	0.0	0.0	22.819125484703694	0.0
24	6.231626438921493E-4	0.0	0.0	23.638116989438952	0.0
25	6.231626438921493E-4	0.0	0.0	24.34493921827362	0.0
26	6.231626438921493E-4	0.0	0.0	25.020291733591737	0.0
27	6.231626438921493E-4	0.0	0.0	25.82463891619553	0.0
28	6.231626438921493E-4	0.0	0.0	26.505444104647705	0.0
29	6.231626438921493E-4	0.0	0.0	27.32895353855118	0.0
30	6.231626438921493E-4	0.0	0.0	28.155578785674113	0.0
31	6.231626438921493E-4	0.0	0.0	28.882965381757224	0.0
32	6.231626438921493E-4	0.0	0.0	29.5637705702094	0.0
33	6.231626438921493E-4	0.0	0.0	30.22603667000578	0.0
34	6.231626438921493E-4	0.0	0.0	31.02259431956092	0.0
35	6.231626438921493E-4	0.0	0.0	31.7663389350462	0.0
36	6.231626438921493E-4	0.0	0.0	32.46241160827373	0.0
37	6.231626438921493E-4	0.0	0.0	33.182164461969165	0.0
38	6.231626438921493E-4	0.0	0.0	34.04430997979395	0.0
39	6.231626438921493E-4	0.0	0.0	35.73183441945389	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.030955E-4	45.000004	24
AAACTCG	20	7.030955E-4	45.000004	17
AACCGGT	20	7.030955E-4	45.000004	39
GTTTCGA	20	7.030955E-4	45.000004	17
CAACGCG	20	7.030955E-4	45.000004	28
ACGTTCG	20	7.030955E-4	45.000004	42
TAGACGC	20	7.030955E-4	45.000004	34
CGGCCGT	20	7.030955E-4	45.000004	6
CGACAAC	20	7.030955E-4	45.000004	11
ATCGTAA	20	7.030955E-4	45.000004	20
GTATCGA	20	7.030955E-4	45.000004	9
TCCGTAG	20	7.030955E-4	45.000004	1
CTCGAGG	20	7.030955E-4	45.000004	1
TCGACGA	20	7.030955E-4	45.000004	12
ATTTGCG	40	6.8066583E-9	45.000004	1
TTCGAAC	20	7.030955E-4	45.000004	38
TGTCGAG	20	7.030955E-4	45.000004	1
TCGCGAT	20	7.030955E-4	45.000004	21
ATCGATT	20	7.030955E-4	45.000004	10
TATCCGG	20	7.030955E-4	45.000004	2
>>END_MODULE