##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934421.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2340585 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.138865710922694 33.0 31.0 34.0 30.0 34.0 2 32.33209689030734 34.0 31.0 34.0 30.0 34.0 3 32.39462399357426 34.0 31.0 34.0 30.0 34.0 4 35.90823020740542 37.0 35.0 37.0 35.0 37.0 5 35.84512760698714 37.0 35.0 37.0 35.0 37.0 6 35.48376794690216 37.0 35.0 37.0 33.0 37.0 7 35.83472251595221 37.0 35.0 37.0 35.0 37.0 8 35.939735578925784 37.0 35.0 37.0 35.0 37.0 9 37.70838871478711 39.0 38.0 39.0 35.0 39.0 10 37.20386698197245 39.0 37.0 39.0 34.0 39.0 11 37.06140088909397 39.0 37.0 39.0 33.0 39.0 12 36.65714938786671 39.0 35.0 39.0 33.0 39.0 13 36.45906942067902 39.0 35.0 39.0 32.0 39.0 14 37.077326394897 39.0 36.0 41.0 31.0 41.0 15 37.2614978733949 40.0 36.0 41.0 31.0 41.0 16 37.4895412898912 40.0 36.0 41.0 32.0 41.0 17 37.51083938417105 39.0 36.0 41.0 33.0 41.0 18 37.51995719018963 39.0 36.0 41.0 33.0 41.0 19 37.5536577394113 39.0 36.0 41.0 33.0 41.0 20 37.43744405778897 39.0 35.0 41.0 32.0 41.0 21 37.27689872403694 39.0 35.0 41.0 32.0 41.0 22 37.47628007528033 39.0 35.0 41.0 33.0 41.0 23 37.45256848181117 39.0 35.0 41.0 33.0 41.0 24 37.38586507219349 39.0 35.0 41.0 32.0 41.0 25 37.31373481415971 39.0 35.0 41.0 32.0 41.0 26 37.24254577381296 39.0 35.0 41.0 32.0 41.0 27 37.163084015320955 39.0 35.0 41.0 32.0 41.0 28 37.03012751085733 39.0 35.0 41.0 32.0 41.0 29 36.917691090048 39.0 35.0 41.0 31.0 41.0 30 36.86407885208185 39.0 35.0 41.0 31.0 41.0 31 36.728250843272086 39.0 35.0 41.0 31.0 41.0 32 36.649702958875665 39.0 35.0 40.0 31.0 41.0 33 36.5954746356146 39.0 35.0 40.0 31.0 41.0 34 36.343711508020434 39.0 35.0 40.0 30.0 41.0 35 36.302017657978666 38.0 35.0 40.0 30.0 41.0 36 36.146017769062006 38.0 35.0 40.0 30.0 41.0 37 36.0634358504391 38.0 35.0 40.0 30.0 41.0 38 35.96548854239432 38.0 35.0 40.0 29.0 41.0 39 35.87849063375182 38.0 35.0 40.0 29.0 41.0 40 35.71341139074206 38.0 34.0 40.0 29.0 41.0 41 35.58427316247861 38.0 34.0 40.0 28.0 41.0 42 35.56375521504239 38.0 34.0 40.0 28.0 41.0 43 35.51205489225984 38.0 34.0 40.0 28.0 41.0 44 35.40652529175399 38.0 34.0 40.0 28.0 41.0 45 35.476466780740715 38.0 34.0 40.0 28.0 41.0 46 35.431393860936474 37.0 34.0 40.0 28.0 41.0 47 35.18675502064655 37.0 34.0 40.0 27.0 41.0 48 35.04733773821502 37.0 34.0 40.0 27.0 41.0 49 35.06466887551616 37.0 34.0 40.0 27.0 41.0 50 34.94469673179996 37.0 34.0 40.0 27.0 41.0 51 33.926214600196104 35.0 32.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 9.0 9 28.0 10 31.0 11 42.0 12 39.0 13 46.0 14 50.0 15 122.0 16 193.0 17 374.0 18 706.0 19 1471.0 20 2341.0 21 3649.0 22 5579.0 23 8158.0 24 11621.0 25 16677.0 26 22212.0 27 27528.0 28 32862.0 29 39703.0 30 48998.0 31 63157.0 32 83495.0 33 120905.0 34 196471.0 35 179947.0 36 200094.0 37 290939.0 38 459108.0 39 523855.0 40 175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.659177086070365 4.5765908950112895 55.39200669917991 15.372225319738442 2 21.485953298000286 4.360149278919586 51.65148883719242 22.502408585887714 3 21.900379605953212 4.39561049908463 50.61337229795115 23.090637597011003 4 20.613650006301842 4.973542939051562 48.50539501876668 25.90741203587992 5 19.53656884924068 5.864516776788708 49.074141720980016 25.524772652990595 6 23.344975721881493 6.268304718692122 52.18994396699971 18.19677559242668 7 83.97631361390422 2.5319311197841565 9.087856241067938 4.403899025243689 8 85.72715795410122 2.729146773135776 7.470141011755609 4.073554261007398 9 80.01995227688803 4.942055084519469 9.584227874655268 5.453764763937221 10 43.74214138772999 25.321062896668995 16.536848693809453 14.39994702179156 11 33.90293452277956 22.641946351019083 24.74475398244456 18.710365143756796 12 30.013436811737236 20.56408974679407 29.581194444978497 19.841278996490193 13 29.2162856721717 19.852173708709575 31.438550618755567 19.492990000363157 14 22.146600102111226 21.920887299542635 35.89790586541399 20.03460673293215 15 20.122618917920093 22.576364455894574 36.55739911176052 20.743617514424812 16 27.24434276046373 22.955115921874235 29.61810829343946 20.182433024222576 17 27.213965739334395 22.985022975025473 27.851541388157237 21.949469897482896 18 28.225678623079276 21.385081080157313 29.914273568360045 20.47496672840337 19 27.118263169250422 23.72846959200371 28.38645039594802 20.766816842797848 20 25.076679548061705 29.030263801570978 27.503466013838423 18.3895906365289 21 25.058863489255888 24.95359066216352 31.98008190260127 18.007463945979318 22 23.50173140475565 21.29954690814476 33.448005519987525 21.75071616711207 23 22.40392893229684 23.348180049004842 32.290346216864585 21.957544801833727 24 22.898677040141674 26.542894190982167 28.17227317102348 22.386155597852675 25 25.520030248848048 24.390739921857143 25.93411476190781 24.155115067387 26 21.64446067970187 24.91847123689163 27.290527795401577 26.14654028800492 27 21.45813973856963 23.453965568436953 31.727110957303413 23.360783735690006 28 19.062114813177047 25.479741175817157 28.990530145241465 26.467613865764328 29 21.3441938660634 26.68849881546707 28.599986755447894 23.367320563021636 30 25.43945210278627 23.490922141259556 29.608110792814617 21.461514963139557 31 24.045057111790427 25.122779134276257 29.66625864901296 21.165905104920352 32 26.165082660958696 24.210998532418177 26.808682444773424 22.81523636184971 33 26.40784248382349 24.01677358438168 26.92976328567431 22.64562064612052 34 21.695131772612402 24.28657792816753 30.96653187130568 23.05175842791439 35 21.5200473385927 26.89374664880788 27.48509453833123 24.101111474268187 36 24.649606829061966 26.1869148097591 26.448217005577668 22.71526135560127 37 22.67475866076216 28.23764144433977 26.87832315425417 22.209276740643897 38 23.71684856563637 26.19199900879481 27.528801560293687 22.562350865275135 39 23.181298692420913 24.148535515693727 27.350171004257483 25.31999478762788 40 25.866610270509295 23.097131699980988 27.512822649038593 23.52343538047112 41 22.56717871814098 22.962806306970265 26.5522081018207 27.917806873068056 42 22.31360963178009 22.749825364171777 29.479980432242364 25.456584571805767 43 21.626516447811124 22.769264948720085 30.620507266345808 24.983711337122987 44 23.05739804365148 22.759993762243198 28.716966057630895 25.465642136474425 45 21.925287908792033 22.43242608151381 26.774716577265938 28.86756943242822 46 21.3421858210661 23.10657378390445 29.195735254220633 26.355505140808816 47 19.45581980573233 25.072193490089017 31.415992155807203 24.055994548371455 48 20.347904476872237 27.092244033008843 29.260548110835536 23.299303379283383 49 23.733382893592843 23.775637287259382 29.28720811250179 23.203771706645988 50 21.3441938660634 24.17643452384767 27.20798432870415 27.271387281384783 51 20.7130695958489 24.47815396578206 26.19071727794547 28.61805916042357 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 431.0 1 1568.0 2 2705.0 3 7291.0 4 11877.0 5 7893.0 6 3909.0 7 3994.5 8 4080.0 9 4319.0 10 4558.0 11 4781.0 12 5004.0 13 5018.0 14 5032.0 15 4904.5 16 4777.0 17 5287.5 18 5798.0 19 5648.5 20 5499.0 21 6211.5 22 6924.0 23 7821.0 24 8718.0 25 10940.0 26 15353.5 27 17545.0 28 21705.0 29 25865.0 30 29745.5 31 33626.0 32 40443.5 33 47261.0 34 52038.5 35 56816.0 36 61202.0 37 65588.0 38 70421.0 39 75254.0 40 81177.0 41 87100.0 42 99397.0 43 111694.0 44 122768.5 45 133843.0 46 156778.5 47 179714.0 48 207347.5 49 234981.0 50 234280.5 51 233580.0 52 211907.0 53 190234.0 54 176209.5 55 162185.0 56 153343.0 57 144501.0 58 134644.0 59 124787.0 60 110403.0 61 96019.0 62 84597.5 63 73176.0 64 63153.5 65 53131.0 66 45753.5 67 38376.0 68 32295.5 69 26215.0 70 22111.5 71 18008.0 72 15056.0 73 12104.0 74 10333.5 75 6290.0 76 4017.0 77 2996.0 78 1975.0 79 1561.0 80 1147.0 81 802.0 82 457.0 83 314.5 84 172.0 85 160.0 86 148.0 87 82.5 88 17.0 89 10.5 90 4.0 91 4.0 92 4.0 93 3.5 94 3.0 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2340585.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.370783208840663 #Duplication Level Percentage of deduplicated Percentage of total 1 71.50556528297834 18.141521950200463 2 11.213960715396226 5.690139324455468 3 4.548415560001378 3.4619059534953776 4 2.390174057944648 2.4256235142203453 5 1.4504560353247244 1.839960281308906 6 0.907806880179347 1.3819062931514523 7 0.6924626739475483 1.2297824266656159 8 0.5252591265455401 1.0660988342441522 9 0.39686920506086554 0.9061994307477734 >10 4.789840602896113 30.631488831206948 >50 1.441342329153137 24.047174420326982 >100 0.12626109150573464 5.532526316166122 >500 0.008519440490171544 1.4486894285428704 >1k 0.0023854433372480326 1.2079904926952127 >5k 6.815552392137236E-4 0.988992502572326 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7340 0.31359681447159576 No Hit CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT 5348 0.22848988607548967 No Hit TCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC 5153 0.2201586355547865 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 5037 0.21520260960400925 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC 3755 0.16042997797559155 No Hit GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC 3717 0.15880645223309559 No Hit CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC 3405 0.1454764513999705 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.1362180822315788E-4 0.0 0.0 0.24754495136899535 0.0 2 2.1362180822315788E-4 0.0 0.0 0.8450878733308126 0.0 3 2.1362180822315788E-4 0.0 0.0 1.2755358169004758 0.0 4 2.1362180822315788E-4 0.0 0.0 1.846162391026175 0.0 5 2.5634616986778945E-4 0.0 0.0 2.7617881854322746 0.0 6 2.5634616986778945E-4 0.0 0.0 3.455418196732868 0.0 7 2.5634616986778945E-4 0.0 0.0 4.0931647430022835 0.0 8 2.5634616986778945E-4 0.0 0.0 5.06945913094376 0.0 9 2.5634616986778945E-4 0.0 0.0 5.632865287951517 0.0 10 2.5634616986778945E-4 0.0 0.0 6.532255824932656 0.0 11 2.5634616986778945E-4 0.0 0.0 8.275965196735005 0.0 12 2.5634616986778945E-4 0.0 0.0 9.660918103807381 0.0 13 2.9907053151242107E-4 0.0 0.0 10.126314575202354 0.0 14 3.4179489315705263E-4 0.0 0.0 10.331306062373296 0.0 15 3.4179489315705263E-4 0.0 0.0 10.577270212361439 0.0 16 3.845192548016842E-4 0.0 0.0 11.126064637686731 0.0 17 4.2724361644631576E-4 0.0 0.0 11.863401670949784 0.0 18 4.2724361644631576E-4 0.0 0.0 12.644659347983517 0.0 19 4.2724361644631576E-4 0.0 0.0 13.223873518799786 0.0 20 4.2724361644631576E-4 0.0 0.0 13.887639201310783 0.0 21 4.2724361644631576E-4 0.0 0.0 14.79301114892217 0.0 22 4.2724361644631576E-4 0.0 0.0 15.745422618704298 0.0 23 4.6996797809094733E-4 0.0 0.0 16.549537829217908 0.0 24 4.6996797809094733E-4 0.0 0.0 17.17369802848433 0.0 25 4.6996797809094733E-4 0.0 0.0 17.74013761516886 0.0 26 4.6996797809094733E-4 0.0 0.0 18.26974880211571 0.0 27 4.6996797809094733E-4 0.0 0.0 18.86964156396798 0.0 28 4.6996797809094733E-4 0.0 0.0 19.43949909958408 0.0 29 4.6996797809094733E-4 0.0 0.0 20.12607959121331 0.0 30 4.6996797809094733E-4 0.0 0.0 20.957452944456193 0.0 31 5.126923397355789E-4 0.0 0.0 21.56208811045102 0.0 32 5.554167013802105E-4 0.0 0.0 22.135705389891843 0.0 33 5.554167013802105E-4 0.0 0.0 22.72602789473572 0.0 34 5.554167013802105E-4 0.0 0.0 23.40192729595379 0.0 35 5.981410630248421E-4 0.0 0.0 24.131659392844096 0.0 36 5.981410630248421E-4 0.0 0.0 24.758083983277686 0.0 37 5.981410630248421E-4 0.0 0.0 25.378612611804314 0.0 38 6.408654246694736E-4 0.0 0.0 26.213147567808903 0.0 39 6.408654246694736E-4 0.0 0.0 27.933102194536836 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTTG 830 0.0 42.56024 1 CGTTTTT 3645 0.0 40.802467 1 CACGACC 710 0.0 38.34507 27 TTTGGGA 10275 0.0 38.145985 4 CGTTTGG 1595 0.0 37.94671 2 TGGGCGA 3055 0.0 37.92962 6 TTTGGGC 4555 0.0 37.44237 4 TTCTTCG 555 0.0 37.2973 1 AACACGT 640 0.0 37.265625 41 GGGCGAT 4665 0.0 37.186493 7 TTGGGAT 5185 0.0 37.058826 5 GCCGATT 195 0.0 36.923077 9 GTTTGCG 405 0.0 36.666664 1 AGACACG 725 0.0 36.62069 24 TCGTTAG 105 0.0 36.42857 1 TTGGGCA 2095 0.0 36.40811 5 TTGGGAC 4130 0.0 36.228813 5 TTGGGAG 5715 0.0 36.220474 5 CGAATAG 230 0.0 36.195656 26 TTTGTCG 785 0.0 36.11465 1 >>END_MODULE