##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934420.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1674239
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.942391140094095	33.0	31.0	34.0	30.0	34.0
2	32.12118401255735	33.0	31.0	34.0	30.0	34.0
3	32.193615129022795	34.0	31.0	34.0	30.0	34.0
4	35.76263185841448	37.0	35.0	37.0	35.0	37.0
5	35.74140430368663	37.0	35.0	37.0	35.0	37.0
6	35.408030753076474	37.0	35.0	37.0	33.0	37.0
7	35.79370388576541	37.0	35.0	37.0	35.0	37.0
8	35.89474859921433	37.0	35.0	37.0	35.0	37.0
9	37.67274684199806	39.0	37.0	39.0	35.0	39.0
10	36.960040352661714	39.0	37.0	39.0	33.0	39.0
11	36.87131108521543	39.0	37.0	39.0	33.0	39.0
12	36.814467946332634	39.0	37.0	39.0	33.0	39.0
13	36.77939171169707	39.0	37.0	39.0	33.0	39.0
14	37.85692783407865	40.0	37.0	41.0	33.0	41.0
15	37.85694933638507	40.0	37.0	41.0	33.0	41.0
16	37.891492194364126	40.0	37.0	41.0	33.0	41.0
17	37.840773629093576	40.0	37.0	41.0	33.0	41.0
18	37.80968009943622	39.0	37.0	41.0	33.0	41.0
19	37.86386889804861	39.0	37.0	41.0	33.0	41.0
20	37.807567497830355	39.0	36.0	41.0	33.0	41.0
21	37.63018720744171	39.0	36.0	41.0	32.0	41.0
22	37.738566596525345	39.0	36.0	41.0	33.0	41.0
23	37.68332418489833	39.0	36.0	41.0	33.0	41.0
24	37.632980715417574	39.0	36.0	41.0	33.0	41.0
25	37.5703659991196	39.0	36.0	41.0	33.0	41.0
26	37.53710551480404	39.0	36.0	41.0	33.0	41.0
27	37.46829455053908	39.0	36.0	41.0	32.0	41.0
28	37.36067132589792	39.0	36.0	41.0	32.0	41.0
29	37.302128310235275	39.0	36.0	41.0	32.0	41.0
30	37.28879090739136	39.0	36.0	41.0	32.0	41.0
31	37.2251482613892	39.0	36.0	41.0	32.0	41.0
32	37.112936086186025	39.0	35.0	41.0	31.0	41.0
33	37.10341892644957	39.0	36.0	41.0	31.0	41.0
34	36.96671681880544	39.0	35.0	41.0	31.0	41.0
35	36.91537647850755	39.0	35.0	41.0	31.0	41.0
36	36.75343484412918	39.0	35.0	40.0	30.0	41.0
37	36.74015896177308	39.0	35.0	40.0	30.0	41.0
38	36.65985262558094	39.0	35.0	40.0	30.0	41.0
39	36.638398102063086	39.0	35.0	40.0	30.0	41.0
40	36.52346349595249	39.0	35.0	40.0	30.0	41.0
41	36.40576703803937	39.0	35.0	40.0	30.0	41.0
42	36.357441798930736	39.0	35.0	40.0	30.0	41.0
43	36.324978094525335	39.0	35.0	40.0	30.0	41.0
44	36.188537000989704	39.0	35.0	40.0	30.0	41.0
45	36.25159789014591	39.0	35.0	40.0	30.0	41.0
46	36.102356354140596	38.0	35.0	40.0	29.0	41.0
47	35.85649121780104	38.0	35.0	40.0	28.0	41.0
48	35.77195131638912	38.0	34.0	40.0	28.0	41.0
49	35.80839593391386	38.0	35.0	40.0	28.0	41.0
50	35.70046809326506	38.0	34.0	40.0	28.0	41.0
51	34.73493987417567	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	19.0
10	29.0
11	27.0
12	9.0
13	10.0
14	11.0
15	21.0
16	43.0
17	120.0
18	241.0
19	472.0
20	967.0
21	1595.0
22	2597.0
23	4162.0
24	6310.0
25	9510.0
26	13192.0
27	16361.0
28	19455.0
29	24427.0
30	30678.0
31	39368.0
32	51244.0
33	68803.0
34	103079.0
35	127741.0
36	155031.0
37	225257.0
38	377676.0
39	395678.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.644510132663257	4.468179274285212	57.90284421758184	12.984466375469692
2	20.67267576492962	4.445124023511577	52.881936211018854	22.000264000539946
3	19.9240371297049	4.70637704652681	52.59535824932999	22.774227574438296
4	18.02317351345895	4.701837670726819	52.59804603763262	24.67694277818161
5	17.245685950452714	5.723077768466748	53.03024239669486	24.000993884385682
6	20.72177269792425	6.116689433229068	55.753927605318	17.40761026352868
7	82.40609614278486	1.8334897227934601	11.004342868610754	4.756071265810915
8	83.91740964103691	1.978092733474731	8.405550223116293	5.698947402372063
9	78.64408844854289	4.270537241098792	11.092562053565828	5.992812256792488
10	35.16976966848819	26.320196817778108	21.58407491403557	16.92595859969813
11	23.729228622675734	27.378707579981114	27.98214591823509	20.90991787910806
12	20.4522771241143	23.713400536004716	34.24391619117701	21.590406148703977
13	21.696125821940594	25.196163749619977	35.0776083940226	18.03010203441683
14	20.39099555081443	26.60295214721435	32.980894603458644	20.025157698512576
15	17.545344481881024	27.777814278606577	34.6793378962024	19.997503343310004
16	19.210280013785365	26.899026960905818	31.952307884358206	21.938385140950604
17	19.083058034127745	27.209317188286736	30.253386762582878	23.454238015002637
18	19.63518948011604	25.93918789372366	33.453049415286586	20.972573210873716
19	20.600463852532407	28.186417829234657	31.086601136396897	20.126517181836046
20	22.1625466853896	28.833876167022748	30.481012567500816	18.522564580086833
21	21.540413286275136	29.339479011061144	30.66414054385306	18.455967158810658
22	19.886228907581298	27.086395669913315	29.425547965374122	23.601827457131268
23	18.512291255907908	27.88855115667476	29.693669780718285	23.905487806699043
24	19.85755916568662	27.641931647751605	29.63686785458946	22.863641331972318
25	18.606423575128762	28.63360607416265	27.794717480598646	24.965252870109943
26	19.241518086724774	28.44785003813673	29.205746610848273	23.104885264290225
27	19.046862484985716	27.549710644657065	30.078979166056936	23.324447704300283
28	17.515121795633718	26.106189140260145	31.71428929800345	24.66439976610269
29	18.52178810791052	24.4628753720347	30.553045294011188	26.462291226043593
30	20.250872187304203	25.307975743009216	29.901107309052055	24.540044760634533
31	18.294341488879425	28.142636744216325	29.655383729563102	23.907638037341144
32	18.118380948000855	29.374539716253175	27.43843620892836	25.06864312681762
33	18.044078533590486	26.13832314263376	29.42220316215307	26.39539516162268
34	16.51383105996217	26.331843900422818	31.494069843074975	25.66025519654004
35	17.921157015217062	25.878204963568525	28.49384108242611	27.706796938788308
36	18.682876220181228	26.254973154967722	29.80589987451015	25.256250750340904
37	15.89307141931349	26.684601182985222	33.066664914626884	24.3556624830744
38	15.545092427066864	28.25845055574503	30.16594404980412	26.03051296738399
39	17.302607333839433	24.85642730816807	31.384288623069946	26.456676734922553
40	19.179997598909114	25.165343777083198	31.41379456576988	24.2408640582378
41	17.56356171370993	27.302075749041805	29.995239628272905	25.13912290897536
42	19.078339472440913	26.023106617394532	30.81005758437117	24.08849632579339
43	19.556228232647786	24.189019608311597	31.22738151482554	25.027370644215075
44	18.506258664384237	23.960438145330507	28.816196492854367	28.717106697430893
45	20.46649253780374	22.646886137522777	27.51972687292555	29.36689445174793
46	20.170059352338583	24.282853284387713	29.66780728438413	25.879280078889572
47	16.613338955788272	25.284801034977683	33.07669932429002	25.025160684944026
48	16.034628269918453	24.910302531478482	32.631959953148865	26.423109245454203
49	18.970230654046404	21.82310888708243	33.3975615189946	25.809098939876563
50	18.378738041581876	21.679580991722208	30.703023881297714	29.238657085398202
51	17.113148122818785	21.531991549593577	29.0828848211038	32.27197550648384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	132.0
1	574.0
2	1016.0
3	3927.5
4	6839.0
5	4671.5
6	2504.0
7	2711.0
8	2918.0
9	3520.5
10	4123.0
11	4619.5
12	5116.0
13	5434.5
14	5753.0
15	5830.0
16	5907.0
17	5825.0
18	5743.0
19	5702.0
20	5661.0
21	6260.5
22	6860.0
23	7648.5
24	8437.0
25	9975.5
26	14206.0
27	16898.0
28	20276.0
29	23654.0
30	27174.5
31	30695.0
32	35075.0
33	39455.0
34	44458.5
35	49462.0
36	55592.0
37	61722.0
38	66105.0
39	70488.0
40	78091.5
41	85695.0
42	95995.0
43	106295.0
44	121200.0
45	136105.0
46	161951.5
47	187798.0
48	213140.0
49	238482.0
50	217699.0
51	196916.0
52	162610.5
53	128305.0
54	106105.0
55	83905.0
56	68573.5
57	53242.0
58	43845.5
59	34449.0
60	28493.5
61	22538.0
62	18899.5
63	15261.0
64	12151.0
65	9041.0
66	6633.5
67	4226.0
68	3530.0
69	2834.0
70	2297.0
71	1760.0
72	1393.5
73	1027.0
74	900.5
75	577.0
76	380.0
77	270.5
78	161.0
79	105.0
80	49.0
81	45.5
82	42.0
83	37.0
84	32.0
85	19.5
86	7.0
87	11.5
88	16.0
89	9.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1674239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.915709683363986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.79300264584309	21.955775006916188
2	13.506850972825477	8.621614687703207
3	5.766433052385697	5.521194096254891
4	2.931639930739589	3.7426147570256814
5	1.7200048958426746	2.7447588454839758
6	1.1380718176114795	2.1793421837823805
7	0.7888728992939906	1.7624206901658452
8	0.5754081138872019	1.4691646649820784
9	0.44554603867204723	1.2797926218746931
>10	3.578751248066097	25.708557659253508
>50	0.6440358249288288	13.629891281384607
>100	0.09965809910053013	5.73833904740005
>500	0.0077532724666896724	1.6629927258192945
>1k	0.0032147715105786453	1.9559507969206542
>5k	5.673126195138785E-4	1.387054468490202
>10k+	1.8910420650462617E-4	0.640536466542795
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	10613	0.6338999390170699	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT	9016	0.5385133185883257	No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	7719	0.46104528684375407	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	6247	0.3731247450334152	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4372	0.2611335657573381	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	3711	0.22165294202321173	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCC	2871	0.1714808937075292	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT	2728	0.16293969976807374	No Hit
TGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	2394	0.1429903376996952	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	1932	0.1153957111260698	No Hit
TTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	1838	0.10978122000502916	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3891451578896442E-4	0.0	0.0	0.5823541322356007	0.0
2	2.3891451578896442E-4	0.0	0.0	2.233014521821556	0.0
3	2.3891451578896442E-4	0.0	0.0	3.223733290169444	0.0
4	2.3891451578896442E-4	0.0	0.0	4.466924973077321	0.0
5	2.3891451578896442E-4	0.0	0.0	6.346584926046998	0.0
6	2.3891451578896442E-4	0.0	0.0	7.5734109646233305	0.0
7	2.3891451578896442E-4	0.0	0.0	8.645778768742097	0.0
8	2.3891451578896442E-4	0.0	0.0	10.352404883651618	0.0
9	2.3891451578896442E-4	0.0	0.0	11.12654764343681	0.0
10	2.3891451578896442E-4	0.0	0.0	12.7850922120438	0.0
11	2.3891451578896442E-4	0.0	0.0	15.911348379771347	0.0
12	2.3891451578896442E-4	0.0	0.0	18.338480945671435	0.0
13	2.3891451578896442E-4	0.0	0.0	19.111070761104	0.0
14	4.181004026306877E-4	0.0	0.0	19.35398709503243	0.0
15	4.181004026306877E-4	0.0	0.0	19.697008611076434	0.0
16	4.181004026306877E-4	0.0	0.0	20.680500215321707	0.0
17	4.181004026306877E-4	0.0	0.0	22.1950390595369	0.0
18	4.7782903157792884E-4	0.0	0.0	23.854539286207046	0.0
19	4.7782903157792884E-4	0.0	0.0	24.965850156399416	0.0
20	5.3755766052517E-4	0.0	0.0	26.01731294038665	0.0
21	5.3755766052517E-4	0.0	0.0	27.61588996553061	0.0
22	5.3755766052517E-4	0.0	0.0	29.322814723584866	0.0
23	5.3755766052517E-4	0.0	0.0	31.16592075563883	0.0
24	5.3755766052517E-4	0.0	0.0	32.40086988775199	0.0
25	5.3755766052517E-4	0.0	0.0	33.46445758341551	0.0
26	5.3755766052517E-4	0.0	0.0	34.38481602686355	0.0
27	5.3755766052517E-4	0.0	0.0	35.30439799813527	0.0
28	5.97286289472411E-4	0.0	0.0	36.33459739021729	0.0
29	5.97286289472411E-4	0.0	0.0	37.37489092059138	0.0
30	6.570149184196521E-4	0.0	0.0	38.46213115331801	0.0
31	6.570149184196521E-4	0.0	0.0	39.48910519943688	0.0
32	6.570149184196521E-4	0.0	0.0	40.439865515019065	0.0
33	6.570149184196521E-4	0.0	0.0	41.35765562742237	0.0
34	6.570149184196521E-4	0.0	0.0	42.245103596320476	0.0
35	6.570149184196521E-4	0.0	0.0	43.13804659908173	0.0
36	6.570149184196521E-4	0.0	0.0	44.013907213964075	0.0
37	6.570149184196521E-4	0.0	0.0	44.88660221151221	0.0
38	6.570149184196521E-4	0.0	0.0	45.74824741270512	0.0
39	6.570149184196521E-4	0.0	0.0	46.725109139137246	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	45	3.8562575E-10	45.000004	41
ATCCCGA	20	7.0342096E-4	45.000004	10
GATATCG	20	7.0342096E-4	45.000004	36
TAGGCGT	20	7.0342096E-4	45.000004	34
ACGCGTG	20	7.0342096E-4	45.000004	44
GTCGTGC	25	3.8915714E-5	45.0	13
TGCACGT	35	1.2123746E-7	45.0	21
ACGGTGA	25	3.8915714E-5	45.0	20
GCGCGAC	180	0.0	43.750004	9
CGTTTTT	2900	0.0	41.50862	1
TACGAAT	125	0.0	41.4	12
GCCTTCG	60	3.6379788E-12	41.249996	1
TGTACGG	60	3.6379788E-12	41.249996	2
AATGCGG	55	6.184564E-11	40.909092	2
TTTCTCG	465	0.0	40.645157	1
TAGCGTG	50	1.0822987E-9	40.5	1
GACGATA	50	1.0822987E-9	40.5	9
GGCGCGA	195	0.0	40.384617	8
CGGGCTA	240	0.0	40.312496	6
CTATGCG	45	1.9292202E-8	40.000004	1
>>END_MODULE