FastQCFastQC Report
Sat 14 Jan 2017
SRR2934419.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934419.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3285203
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291590.8875859421776979No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT61400.1868986482722681No Hit
TCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC39700.12084489147245998No Hit
GCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC35460.1079385353051242No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG35190.10711666828503444No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC35170.10705578924650927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT168550.043.3313561
TCTAGCG1000.040.51
TACGGGA7050.039.255324
TCTTGCG13850.038.989171
TGGGCGA38950.038.761236
TTTGGGA243100.038.5026744
CGTTTGG22800.038.190792
TTCTTCG9800.038.1122441
TTGGGAT165400.037.7221875
TTGGGAC96050.037.3399245
TTCGGGA43350.036.9550174
GGGCGAT59600.036.8078847
TTCGTTG13400.036.6044771
TTGGGCG30150.036.5671655
TTTCGCG11200.036.56251
GACCGAT14600.036.523979
TTTTGCG26800.036.5205231
TCTGGGA115900.036.516394
TGCGGGA58800.036.198984
TTTGGGC56750.036.198244
Produced by FastQC (version 0.11.5)