##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934419.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3285203
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.236618559035776	33.0	31.0	34.0	30.0	34.0
2	32.43983065886644	34.0	31.0	34.0	31.0	34.0
3	32.4704814283927	34.0	31.0	34.0	30.0	34.0
4	35.986589260998485	37.0	35.0	37.0	35.0	37.0
5	35.952716468358275	37.0	35.0	37.0	35.0	37.0
6	35.62810395582861	37.0	35.0	37.0	35.0	37.0
7	35.94887469663214	37.0	35.0	37.0	35.0	37.0
8	36.04452631998692	37.0	35.0	37.0	35.0	37.0
9	37.83299996986488	39.0	38.0	39.0	35.0	39.0
10	37.33121210470099	39.0	37.0	39.0	34.0	39.0
11	37.23253266236516	39.0	37.0	39.0	34.0	39.0
12	37.038541301709515	39.0	37.0	39.0	33.0	39.0
13	36.963227538754836	39.0	37.0	39.0	33.0	39.0
14	37.975103821590324	40.0	37.0	41.0	33.0	41.0
15	38.060594429020064	40.0	37.0	41.0	33.0	41.0
16	38.1450820542901	40.0	37.0	41.0	33.0	41.0
17	38.11623147793302	40.0	37.0	41.0	33.0	41.0
18	38.035875104217304	40.0	37.0	41.0	33.0	41.0
19	38.00931023136165	40.0	37.0	41.0	33.0	41.0
20	37.86988901446882	40.0	36.0	41.0	33.0	41.0
21	37.727379403951595	40.0	36.0	41.0	33.0	41.0
22	37.85157599089006	40.0	36.0	41.0	33.0	41.0
23	37.815901483104696	40.0	36.0	41.0	33.0	41.0
24	37.76547385351834	40.0	36.0	41.0	33.0	41.0
25	37.69202207595695	40.0	35.0	41.0	33.0	41.0
26	37.634430809907336	40.0	35.0	41.0	33.0	41.0
27	37.556925097170556	39.0	35.0	41.0	33.0	41.0
28	37.44519745050763	39.0	35.0	41.0	33.0	41.0
29	37.35929408319669	39.0	35.0	41.0	33.0	41.0
30	37.27032241234408	39.0	35.0	41.0	32.0	41.0
31	37.117390919221734	39.0	35.0	41.0	32.0	41.0
32	36.93121703590311	39.0	35.0	41.0	31.0	41.0
33	36.75181229287809	39.0	35.0	41.0	31.0	41.0
34	36.480558735639775	39.0	35.0	41.0	30.0	41.0
35	36.3439665676672	39.0	35.0	41.0	30.0	41.0
36	36.15356646149416	39.0	35.0	41.0	29.0	41.0
37	36.06274650303193	39.0	35.0	41.0	29.0	41.0
38	35.99089158265106	39.0	35.0	41.0	29.0	41.0
39	35.912984068260016	39.0	35.0	41.0	28.0	41.0
40	35.75430102797301	39.0	35.0	41.0	27.0	41.0
41	35.630322996782844	39.0	35.0	40.0	26.0	41.0
42	35.58564447919961	39.0	35.0	40.0	26.0	41.0
43	35.5225877974664	38.0	35.0	40.0	26.0	41.0
44	35.406837263937724	38.0	34.0	40.0	26.0	41.0
45	35.48096479882674	38.0	34.0	40.0	26.0	41.0
46	35.419959131901436	38.0	34.0	40.0	26.0	41.0
47	35.21726237313189	38.0	34.0	40.0	26.0	41.0
48	35.09985592975533	38.0	34.0	40.0	25.0	41.0
49	35.07990982596814	38.0	34.0	40.0	26.0	41.0
50	34.95443904075334	38.0	34.0	40.0	25.0	41.0
51	34.04221109015181	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	36.0
10	65.0
11	53.0
12	45.0
13	39.0
14	69.0
15	105.0
16	195.0
17	440.0
18	836.0
19	1625.0
20	2939.0
21	4634.0
22	7208.0
23	11347.0
24	18021.0
25	28714.0
26	41064.0
27	48218.0
28	50892.0
29	54473.0
30	62024.0
31	75399.0
32	96049.0
33	132416.0
34	205440.0
35	224173.0
36	260140.0
37	388203.0
38	658129.0
39	911874.0
40	331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.906286156441475	4.068759221271867	55.1964368716332	17.82851775065346
2	22.905829563652535	3.922071177945472	52.20450608379452	20.967593174607472
3	20.1695602981003	4.144218789523813	53.81061687816552	21.875604034210365
4	18.222466008949826	4.537436499357878	53.09824080886326	24.141856682829037
5	17.949058246933294	5.413424984696532	52.68694202458721	23.95057474378296
6	21.358771436650947	5.732187630414316	54.89274178795039	18.016299144984345
7	82.98312159096409	1.8755614188833993	11.289165387953195	3.8521516021993163
8	85.56177502577466	2.0265109949065554	9.357717011703691	3.053996967615091
9	80.26639449677843	4.860673754407261	10.933144770658009	3.939786978156297
10	35.74363593360898	36.17000227991999	16.691053794849207	11.395307991621827
11	25.56067311517736	23.031605657245535	33.04812518434934	18.359596043227768
12	24.037114297046482	21.907869924628706	34.82040531437479	19.234610463950023
13	24.12197967675057	20.912832479454085	36.0871763480065	18.878011495788844
14	19.65202759159784	23.607795317366996	36.52940168385332	20.21077540718184
15	18.304530952881755	24.817461812862096	37.50937156699297	19.368635667263177
16	23.701183762464602	24.92126057354751	32.93863423356182	18.438921430426063
17	23.782579036972752	24.37852394509563	32.42740250754672	19.411494510384898
18	23.663164802905634	23.665660843485167	33.56300965267595	19.108164700933244
19	23.24523020343035	25.548771263145685	31.812615537000298	19.39338299642366
20	23.125268058016506	28.34287561529683	31.154056537754286	17.377799788932375
21	23.834417538276934	25.47751234855198	33.469469009982035	17.21860110318906
22	22.59169372486266	22.57869605013754	34.722907534176734	20.106702690823063
23	21.08177181136143	24.120305503191126	34.25989200667356	20.538030678773882
24	20.980195135582186	25.43276625523598	32.73523736584924	20.851801243332606
25	22.022718230806436	26.266626445915215	30.617042538923773	21.093612784354573
26	20.652057117931523	26.44311477859968	30.837759493096772	22.067068610372022
27	19.741671975826154	25.470085105851908	34.00724399679411	20.780998921527832
28	18.735341469005114	26.053214976365236	32.492634397326434	22.718809157303216
29	20.93505332851577	25.774754254151112	32.00968098470627	21.28051143262684
30	22.904307587689406	24.40302775810201	32.568276602693956	20.124388051514625
31	21.990087066156946	26.248210536761352	31.581001234931293	20.180701162150406
32	23.556230771736175	26.151138909832973	29.57902449255038	20.71360582588047
33	23.479310106559627	25.425612968209272	29.991419099519877	21.103657825711228
34	21.25609893817825	26.17025492792987	31.543621505276842	21.030024628615035
35	21.132149215741006	27.626694606086748	29.954222006981002	21.286934171191245
36	23.16252602959391	27.762972333825335	28.879920053646607	20.194581582934145
37	21.17199454645573	29.150101226621306	29.065662000186897	20.612242226736065
38	21.937000544563	27.985789614827457	28.68860767508127	21.38860216552828
39	21.984638392208943	26.16858075437043	28.523929875870685	23.32285097754994
40	23.418004914764783	26.220693211347974	29.512209747769013	20.849092126118236
41	20.95739593565451	27.044051767881623	29.12517126034525	22.87338103611862
42	21.540464927129314	26.387958369695873	30.452760453463608	21.618816249711205
43	21.28471208628508	25.236066081761155	31.113967690885463	22.3652541410683
44	22.13254401630584	25.12277019106582	29.563713414361303	23.180972378267036
45	21.644842038680714	24.515684418892835	28.80029027125569	25.039183271170764
46	21.289399772251517	25.5307815072615	30.07613228162765	23.103686438859334
47	19.96025816365077	26.583988873746918	31.357788240178763	22.097964722423548
48	20.267879945318448	27.096468620051787	30.47081108838632	22.164840346243444
49	22.543294889235156	25.136315777137668	30.36421189192875	21.956177441698426
50	21.010604215325507	25.429174391962995	29.25414350346082	24.30607788925068
51	20.39508669631679	25.75655142163209	29.09381855550479	24.754543326546337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	470.0
1	1876.5
2	3283.0
3	25654.5
4	48026.0
5	32082.5
6	16139.0
7	15839.5
8	15540.0
9	15830.5
10	16121.0
11	15648.5
12	15176.0
13	14937.5
14	14699.0
15	14132.0
16	13565.0
17	13378.5
18	13192.0
19	13564.5
20	13937.0
21	14693.0
22	15449.0
23	18744.0
24	22039.0
25	26455.0
26	38124.5
27	45378.0
28	51791.5
29	58205.0
30	66264.0
31	74323.0
32	83553.0
33	92783.0
34	104064.0
35	115345.0
36	122220.5
37	129096.0
38	136391.0
39	143686.0
40	155207.0
41	166728.0
42	176231.5
43	185735.0
44	197202.0
45	208669.0
46	231211.0
47	253753.0
48	273331.5
49	292910.0
50	287336.0
51	281762.0
52	254827.5
53	227893.0
54	207110.0
55	186327.0
56	170306.0
57	154285.0
58	139527.0
59	124769.0
60	110204.5
61	95640.0
62	83196.5
63	70753.0
64	60544.0
65	50335.0
66	42137.0
67	33939.0
68	27012.0
69	20085.0
70	16384.0
71	12683.0
72	11023.5
73	9364.0
74	7714.0
75	4614.0
76	3164.0
77	2466.5
78	1769.0
79	1208.0
80	647.0
81	520.0
82	393.0
83	257.0
84	121.0
85	82.5
86	44.0
87	35.0
88	26.0
89	20.0
90	14.0
91	10.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3285203.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.318876296555484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.71210676054083	19.40019819389165
2	10.140940063197048	5.337962941081333
3	3.7960446438180613	2.9972288819054866
4	1.9781301195830276	2.0824864766318485
5	1.2102368729634465	1.5926037274527536
6	0.8258708819163592	1.3041596146850303
7	0.5777773367313523	1.0644515176670022
8	0.4661732372304192	0.9815324610745771
9	0.3903395253340649	0.9245967912831095
>10	5.566846574323083	35.12144805706458
>50	1.2245005259833404	21.64195564099476
>100	0.1036433944259897	4.437490723203659
>500	0.004809406699509957	0.7932438430522956
>1k	0.0023460520485400886	1.2308647458020154
>5k	1.1730260242700442E-4	0.189558542707978
>10k+	1.1730260242700442E-4	0.900217841501943
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29159	0.8875859421776979	No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT	6140	0.1868986482722681	No Hit
TCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC	3970	0.12084489147245998	No Hit
GCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC	3546	0.1079385353051242	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	3519	0.10711666828503444	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC	3517	0.10705578924650927	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8263711557550628E-4	0.0	0.0	0.20832806983312752	0.0
2	1.8263711557550628E-4	0.0	0.0	0.5836169028215303	0.0
3	1.8263711557550628E-4	0.0	0.0	0.8691700330238344	0.0
4	1.8263711557550628E-4	0.0	0.0	1.2448241402433884	0.0
5	1.8263711557550628E-4	0.0	0.0	1.946181103572595	0.0
6	1.8263711557550628E-4	0.0	0.0	2.667171556826169	0.0
7	1.8263711557550628E-4	0.0	0.0	3.2828412734312002	0.0
8	1.8263711557550628E-4	0.0	0.0	4.142361978848796	0.0
9	2.1307663483809068E-4	0.0	0.0	4.717303618680489	0.0
10	2.1307663483809068E-4	0.0	0.0	5.5504941399359495	0.0
11	2.1307663483809068E-4	0.0	0.0	6.807494087884371	0.0
12	2.1307663483809068E-4	0.0	0.0	7.822438978656722	0.0
13	2.1307663483809068E-4	0.0	0.0	8.171458506521514	0.0
14	2.4351615410067506E-4	0.0	0.0	8.366728022590994	0.0
15	2.4351615410067506E-4	0.0	0.0	8.554387658844826	0.0
16	2.4351615410067506E-4	0.0	0.0	8.912721679603969	0.0
17	2.4351615410067506E-4	0.0	0.0	9.467542797203095	0.0
18	2.7395567336325945E-4	0.0	0.0	10.04921157079182	0.0
19	2.7395567336325945E-4	0.0	0.0	10.479991647395915	0.0
20	2.7395567336325945E-4	0.0	0.0	10.958927043473418	0.0
21	2.7395567336325945E-4	0.0	0.0	11.594747721830279	0.0
22	3.043951926258438E-4	0.0	0.0	12.330196946733581	0.0
23	3.043951926258438E-4	0.0	0.0	13.011402948310957	0.0
24	3.043951926258438E-4	0.0	0.0	13.555813750322278	0.0
25	3.043951926258438E-4	0.0	0.0	14.02354740331115	0.0
26	3.043951926258438E-4	0.0	0.0	14.446017491156558	0.0
27	3.043951926258438E-4	0.0	0.0	14.924526733964385	0.0
28	3.6527423115101257E-4	0.0	0.0	15.395030383206151	0.0
29	3.6527423115101257E-4	0.0	0.0	15.923582195681668	0.0
30	3.6527423115101257E-4	0.0	0.0	16.551092885279843	0.0
31	3.9571375041359694E-4	0.0	0.0	17.05313187647765	0.0
32	3.9571375041359694E-4	0.0	0.0	17.54466923352986	0.0
33	3.9571375041359694E-4	0.0	0.0	18.03803296173783	0.0
34	3.9571375041359694E-4	0.0	0.0	18.573433666047425	0.0
35	3.9571375041359694E-4	0.0	0.0	19.15330650799966	0.0
36	3.9571375041359694E-4	0.0	0.0	19.677414150662837	0.0
37	4.2615326967618137E-4	0.0	0.0	20.188889392832042	0.0
38	4.2615326967618137E-4	0.0	0.0	20.804833065110437	0.0
39	4.2615326967618137E-4	0.0	0.0	21.813294338279857	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	16855	0.0	43.331356	1
TCTAGCG	100	0.0	40.5	1
TACGGGA	705	0.0	39.25532	4
TCTTGCG	1385	0.0	38.98917	1
TGGGCGA	3895	0.0	38.76123	6
TTTGGGA	24310	0.0	38.502674	4
CGTTTGG	2280	0.0	38.19079	2
TTCTTCG	980	0.0	38.112244	1
TTGGGAT	16540	0.0	37.722187	5
TTGGGAC	9605	0.0	37.339924	5
TTCGGGA	4335	0.0	36.955017	4
GGGCGAT	5960	0.0	36.807884	7
TTCGTTG	1340	0.0	36.604477	1
TTGGGCG	3015	0.0	36.567165	5
TTTCGCG	1120	0.0	36.5625	1
GACCGAT	1460	0.0	36.52397	9
TTTTGCG	2680	0.0	36.520523	1
TCTGGGA	11590	0.0	36.51639	4
TGCGGGA	5880	0.0	36.19898	4
TTTGGGC	5675	0.0	36.19824	4
>>END_MODULE