FastQCFastQC Report
Sat 14 Jan 2017
SRR2934417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405058
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90930.3780782001930931No Hit
CTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGCT34340.14278241938448055Illumina PCR Primer Index 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC33840.1407034674423652TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC32790.13633766836392305TruSeq Adapter, Index 21 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24510.10191022420249325No Hit
GCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC24420.1015360128529125TruSeq Adapter, Index 21 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGA207.0348254E-445.03
CGTTTTT46250.041.5459481
TTTGGGA175200.038.9897234
TTTCGCG9200.038.885871
TTTTGCG21600.038.3333321
CGGTAGT4000.038.25000412
TTGGGAC71050.038.1597485
TGGGCGA28650.037.85346
TTGGGAT121150.037.5154765
GTATGCG301.140293E-437.4999961
GGGCGAT43900.037.465837
TATTGCG1700.037.0588261
TAGCGGG5450.036.3302733
TATGCGG3100.036.290322
TGCGGGA42500.036.158824
GTTTACG1000.036.0000041
ATTACGC250.00210756536.00000426
TACGGTA250.00210756536.00000424
TTGGGCG21550.035.9164735
TGTTTCG5200.035.9134641
Produced by FastQC (version 0.11.5)