##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934417.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2405058
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36003331312592	33.0	31.0	34.0	31.0	34.0
2	32.55271016333078	34.0	31.0	34.0	31.0	34.0
3	32.61005098421743	34.0	31.0	34.0	31.0	34.0
4	36.07215460084539	37.0	35.0	37.0	35.0	37.0
5	36.02461354362348	37.0	35.0	37.0	35.0	37.0
6	35.68482506450988	37.0	35.0	37.0	35.0	37.0
7	36.00371591870134	37.0	35.0	37.0	35.0	37.0
8	36.09987160392806	37.0	35.0	37.0	35.0	37.0
9	37.90099032954715	39.0	38.0	39.0	35.0	39.0
10	37.425309077785236	39.0	37.0	39.0	35.0	39.0
11	37.28396071945043	39.0	37.0	39.0	34.0	39.0
12	37.0940983543848	39.0	37.0	39.0	34.0	39.0
13	37.044637592939544	39.0	37.0	39.0	33.0	39.0
14	38.11080522798203	40.0	38.0	41.0	33.0	41.0
15	38.19319575660961	40.0	37.0	41.0	33.0	41.0
16	38.25549113576471	40.0	37.0	41.0	33.0	41.0
17	38.22028242146343	40.0	37.0	41.0	33.0	41.0
18	38.168057485515945	40.0	37.0	41.0	33.0	41.0
19	38.190586256131866	40.0	37.0	41.0	34.0	41.0
20	38.12747925413857	40.0	37.0	41.0	34.0	41.0
21	38.000278163769856	40.0	37.0	41.0	33.0	41.0
22	38.09612159041487	40.0	37.0	41.0	34.0	41.0
23	38.05627473433073	40.0	37.0	41.0	34.0	41.0
24	38.01575637676929	40.0	36.0	41.0	34.0	41.0
25	37.9470786151519	40.0	36.0	41.0	34.0	41.0
26	37.91610555753749	40.0	36.0	41.0	34.0	41.0
27	37.84939406866695	40.0	36.0	41.0	33.0	41.0
28	37.74732917043996	40.0	36.0	41.0	33.0	41.0
29	37.68097692446502	40.0	36.0	41.0	33.0	41.0
30	37.64770371442186	40.0	36.0	41.0	33.0	41.0
31	37.55587931767134	40.0	36.0	41.0	33.0	41.0
32	37.4396226619067	40.0	36.0	41.0	33.0	41.0
33	37.37177149158149	40.0	35.0	41.0	32.0	41.0
34	37.209839430067795	40.0	35.0	41.0	32.0	41.0
35	37.11625956629736	40.0	35.0	41.0	31.0	41.0
36	36.99300391092439	39.0	35.0	41.0	31.0	41.0
37	36.98460328191669	39.0	35.0	41.0	31.0	41.0
38	36.92510450891413	39.0	35.0	41.0	31.0	41.0
39	36.87036736743979	39.0	35.0	41.0	31.0	41.0
40	36.755455793581696	39.0	35.0	41.0	31.0	41.0
41	36.61899713021474	39.0	35.0	41.0	31.0	41.0
42	36.56775595432626	39.0	35.0	41.0	30.0	41.0
43	36.55054639014943	39.0	35.0	41.0	30.0	41.0
44	36.45076459694527	39.0	35.0	41.0	30.0	41.0
45	36.48767846762947	39.0	35.0	41.0	31.0	41.0
46	36.41159506340388	39.0	35.0	41.0	30.0	41.0
47	36.20025463003387	38.0	35.0	40.0	30.0	41.0
48	36.107779521325476	38.0	35.0	40.0	30.0	41.0
49	36.106369160327944	38.0	35.0	40.0	30.0	41.0
50	36.00876527717835	38.0	35.0	40.0	29.0	41.0
51	35.1116072876413	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	22.0
10	40.0
11	35.0
12	36.0
13	44.0
14	36.0
15	72.0
16	131.0
17	242.0
18	453.0
19	832.0
20	1433.0
21	2342.0
22	3565.0
23	5338.0
24	8280.0
25	12539.0
26	17399.0
27	21283.0
28	24497.0
29	29358.0
30	36143.0
31	46040.0
32	61665.0
33	89523.0
34	153615.0
35	167361.0
36	183938.0
37	281739.0
38	506831.0
39	749958.0
40	262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.825003804482055	4.249668822955622	57.078997679058055	16.846329693504273
2	19.791040382394105	4.320644242259438	54.96337302468382	20.924942350662647
3	19.707258619126858	4.4288329013271195	53.72976452127142	22.134143958274603
4	18.773227090573283	4.641759159238571	52.07159245224023	24.513421297947907
5	18.383465180465503	5.516956347830281	51.557176583683216	24.542401888020997
6	21.68662876321486	6.023555357084944	53.695919183653785	18.593896696046418
7	85.89277264831036	1.8819504560804772	8.384246866395738	3.8410300292134325
8	88.77781741646147	2.295994524872165	6.040062235505339	2.8861258231610214
9	83.61162184030489	5.1044507034757585	7.432211614023446	3.8517158421959055
10	39.148286652546425	35.71402436032728	13.622956286293302	11.514732700832994
11	26.74426146895418	24.598117800069687	29.745353334514178	18.912267396461957
12	26.344894800873824	21.91489768645912	31.943304485796187	19.79690302687087
13	24.980062850875115	21.998804186842897	32.643162867589886	20.377970094692103
14	20.08608524201911	24.5839809268633	33.03255056634809	22.2973832647695
15	18.652439982736382	25.781748298793627	35.04306341052898	20.522748307941015
16	24.28103604985826	25.47090340440854	31.487598220084507	18.760462325648692
17	24.1938032263671	24.95366016121025	29.741029114474575	21.11150749794807
18	23.621550914780435	24.36564939390235	31.464272379293973	20.548527312023243
19	24.35463094860914	26.185854977302004	29.720114857936895	19.73939921615196
20	25.06579882896795	28.01080057112968	28.81830708448611	18.10509351541626
21	25.274151392606747	26.21990821011385	30.513941867514216	17.991998529765183
22	23.81959187678634	23.651321506591525	31.50044614308678	21.028640473535358
23	22.254265801490025	25.175525912472796	31.485186635831653	21.085021650205526
24	22.7729643110478	25.319722019177917	30.670736422988554	21.236577246785732
25	23.190376281985714	26.458779788262905	28.094457597280396	22.256386332470985
26	21.08851428946828	26.910452887206876	28.418940416405757	23.582092406919084
27	20.09340315285536	26.44327080677472	31.91257757609172	21.5507484642782
28	19.309763007794405	26.63806860375093	30.981622896412475	23.07054549204219
29	20.849102183814278	26.491668807987168	30.136570510981443	22.522658497217115
30	23.625584081548137	24.95149805119045	30.318395647838848	21.104522219422567
31	23.360185076617697	25.830769985588702	29.781776572540036	21.027268365253562
32	24.055137131828005	25.62125320886232	29.22835956554894	21.095250093760733
33	24.23417647308298	25.20163754886576	29.330851896295222	21.233334081756034
34	22.399418226088518	24.841854125763287	31.427724404151586	21.33100324399661
35	21.02997100277831	25.83301525368619	31.01355559824337	22.12345814529213
36	22.23917261039027	27.008246786563983	29.830340889907852	20.922239713137895
37	22.163207706425375	27.759247386133723	29.43026737816718	20.647277529273723
38	22.835707080660843	26.451836088776236	28.48584108990303	22.22661574065989
39	22.750719525267165	25.180848029444615	28.486631091641033	23.58180135364719
40	24.755868673437398	24.633210508852592	29.48619118541008	21.12472963229993
41	21.011260435299274	24.963805446687772	30.407457949039067	23.617476168973887
42	20.67176758315184	25.235108675133823	31.377496925230076	22.71562681648426
43	21.064481605017427	24.694206958834254	31.07592415650683	23.16538727964149
44	22.176679315010283	24.4275190036997	29.648016804584337	23.747784876705676
45	22.40453244786612	23.4352768207669	28.754192206591277	25.405998524775704
46	22.150983469005737	24.485604920962405	29.66697684629643	23.69643476373543
47	20.257182986855202	25.018856094114987	32.127416469789914	22.596544449239893
48	20.73937510030943	24.99602920179056	31.468471862217047	22.796123835682966
49	22.300044323255406	23.851441420539548	31.08295101407118	22.76556324213387
50	21.44168664539483	24.14769207229098	30.063141928385928	24.347479353928264
51	20.464205021251047	23.899132578091674	29.85520515513555	25.78145724552173
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	317.0
1	1377.5
2	2438.0
3	8959.5
4	15481.0
5	10290.0
6	5099.0
7	5069.5
8	5040.0
9	5076.0
10	5112.0
11	5102.5
12	5093.0
13	4937.5
14	4782.0
15	5120.0
16	5458.0
17	5290.5
18	5123.0
19	6055.0
20	6987.0
21	8376.0
22	9765.0
23	12066.5
24	14368.0
25	16645.0
26	25471.5
27	32021.0
28	36859.5
29	41698.0
30	47607.5
31	53517.0
32	61952.0
33	70387.0
34	79489.5
35	88592.0
36	93894.5
37	99197.0
38	106181.0
39	113165.0
40	120554.5
41	127944.0
42	137574.0
43	147204.0
44	150957.0
45	154710.0
46	166892.0
47	179074.0
48	193487.5
49	207901.0
50	203618.5
51	199336.0
52	179537.5
53	159739.0
54	145884.5
55	132030.0
56	126294.0
57	120558.0
58	113495.5
59	106433.0
60	97249.5
61	88066.0
62	76829.0
63	65592.0
64	54543.0
65	43494.0
66	35423.5
67	27353.0
68	22233.0
69	17113.0
70	13953.0
71	10793.0
72	8370.0
73	5947.0
74	5069.0
75	3564.5
76	2938.0
77	2059.5
78	1181.0
79	852.0
80	523.0
81	375.0
82	227.0
83	154.0
84	81.0
85	61.5
86	42.0
87	28.5
88	15.0
89	10.5
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2405058.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.90364464880648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6594626482359	17.368259755166584
2	10.983525585924939	5.250925851940474
3	4.001418251778938	2.8694543994531654
4	1.9785402067289477	1.891772881000995
5	1.1216181822406814	1.340538122996076
6	0.7354206555000878	1.0547540409879859
7	0.5552495452746574	0.9290741475159755
8	0.41938472009009875	0.8019858656138313
9	0.35638509680527675	0.7667012440947448
>10	5.272945385011603	33.26235142496077
>50	1.804833208677116	28.48595972703693
>100	0.10557650204050971	4.03926153603114
>500	0.0033487569416936285	0.5289032969208562
>1k	0.0021150043842275545	1.026967242770654
>5k	1.7625036535229622E-4	0.38309046350974557
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9093	0.3780782001930931	No Hit
CTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGCT	3434	0.14278241938448055	Illumina PCR Primer Index 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC	3384	0.1407034674423652	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC	3279	0.13633766836392305	TruSeq Adapter, Index 21 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2451	0.10191022420249325	No Hit
GCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC	2442	0.1015360128529125	TruSeq Adapter, Index 21 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6631615536922601E-4	0.0	0.0	0.1557966585421225	0.0
2	1.6631615536922601E-4	0.0	0.0	0.5792375901121719	0.0
3	1.6631615536922601E-4	0.0	0.0	0.8856751063799709	0.0
4	1.6631615536922601E-4	0.0	0.0	1.245167476210553	0.0
5	1.6631615536922601E-4	0.0	0.0	1.845859850365355	0.0
6	1.6631615536922601E-4	0.0	0.0	2.3250166939840953	0.0
7	1.6631615536922601E-4	0.0	0.0	2.743052350504645	0.0
8	1.6631615536922601E-4	0.0	0.0	3.353141587437808	0.0
9	1.6631615536922601E-4	0.0	0.0	3.70577341585941	0.0
10	1.6631615536922601E-4	0.0	0.0	4.282848895951782	0.0
11	1.6631615536922601E-4	0.0	0.0	5.3644444333567005	0.0
12	1.6631615536922601E-4	0.0	0.0	6.28379856119894	0.0
13	1.6631615536922601E-4	0.0	0.0	6.5814213212321695	0.0
14	1.6631615536922601E-4	0.0	0.0	6.705784226409508	0.0
15	1.6631615536922601E-4	0.0	0.0	6.865322998447438	0.0
16	1.6631615536922601E-4	0.0	0.0	7.211260601615429	0.0
17	1.6631615536922601E-4	0.0	0.0	7.7407280822333595	0.0
18	2.0789519421153253E-4	0.0	0.0	8.302751950264817	4.1579038842306504E-5
19	2.0789519421153253E-4	0.0	0.0	8.747148717411388	4.1579038842306504E-5
20	2.9105327189614557E-4	0.0	0.0	9.22792714354498	4.1579038842306504E-5
21	3.3263231073845203E-4	0.0	0.0	9.867786972289235	4.1579038842306504E-5
22	3.3263231073845203E-4	0.0	0.0	10.564901137519344	4.1579038842306504E-5
23	3.3263231073845203E-4	0.0	0.0	11.223596270859165	4.1579038842306504E-5
24	3.3263231073845203E-4	0.0	0.0	11.731442651279096	4.1579038842306504E-5
25	4.1579038842306506E-4	0.0	0.0	12.171639935502595	4.1579038842306504E-5
26	4.1579038842306506E-4	0.0	0.0	12.562815532931015	4.1579038842306504E-5
27	4.1579038842306506E-4	0.0	0.0	12.997441225949645	4.1579038842306504E-5
28	4.1579038842306506E-4	0.0	0.0	13.421589001180013	4.1579038842306504E-5
29	4.1579038842306506E-4	0.0	0.0	13.91816746207368	4.1579038842306504E-5
30	4.1579038842306506E-4	0.0	0.0	14.473164472540788	4.1579038842306504E-5
31	4.1579038842306506E-4	0.0	0.0	14.945252879556335	4.1579038842306504E-5
32	4.1579038842306506E-4	0.0	0.0	15.392061230955761	4.1579038842306504E-5
33	4.573694272653716E-4	0.0	0.0	15.856457515785483	4.1579038842306504E-5
34	4.573694272653716E-4	0.0	0.0	16.351871763591564	4.1579038842306504E-5
35	4.573694272653716E-4	0.0	0.0	16.88898978735648	4.1579038842306504E-5
36	4.573694272653716E-4	0.0	0.0	17.34818869233091	4.1579038842306504E-5
37	4.573694272653716E-4	0.0	0.0	17.828509749037238	4.1579038842306504E-5
38	4.573694272653716E-4	0.0	0.0	18.373111999793768	4.1579038842306504E-5
39	4.573694272653716E-4	0.0	0.0	19.215087536350474	4.1579038842306504E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGGA	20	7.0348254E-4	45.0	3
CGTTTTT	4625	0.0	41.545948	1
TTTGGGA	17520	0.0	38.989723	4
TTTCGCG	920	0.0	38.88587	1
TTTTGCG	2160	0.0	38.333332	1
CGGTAGT	400	0.0	38.250004	12
TTGGGAC	7105	0.0	38.159748	5
TGGGCGA	2865	0.0	37.8534	6
TTGGGAT	12115	0.0	37.515476	5
GTATGCG	30	1.140293E-4	37.499996	1
GGGCGAT	4390	0.0	37.46583	7
TATTGCG	170	0.0	37.058826	1
TAGCGGG	545	0.0	36.330273	3
TATGCGG	310	0.0	36.29032	2
TGCGGGA	4250	0.0	36.15882	4
GTTTACG	100	0.0	36.000004	1
ATTACGC	25	0.002107565	36.000004	26
TACGGTA	25	0.002107565	36.000004	24
TTGGGCG	2155	0.0	35.916473	5
TGTTTCG	520	0.0	35.913464	1
>>END_MODULE