##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934416.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426799
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18457166019602	33.0	31.0	34.0	30.0	34.0
2	32.370549134370044	34.0	31.0	34.0	30.0	34.0
3	32.4470254147737	34.0	31.0	34.0	30.0	34.0
4	35.93462730699931	37.0	35.0	37.0	35.0	37.0
5	35.88724434687054	37.0	35.0	37.0	35.0	37.0
6	35.534026555826046	37.0	35.0	37.0	33.0	37.0
7	35.81439272350685	37.0	35.0	37.0	35.0	37.0
8	35.91316989964831	37.0	35.0	37.0	35.0	37.0
9	37.69738448309391	39.0	38.0	39.0	35.0	39.0
10	37.23536137619816	39.0	37.0	39.0	34.0	39.0
11	37.131281938336315	39.0	37.0	39.0	34.0	39.0
12	36.919613213714186	39.0	37.0	39.0	33.0	39.0
13	36.811440514153034	39.0	37.0	39.0	33.0	39.0
14	37.78413023460692	40.0	37.0	41.0	33.0	41.0
15	37.88694678291186	40.0	37.0	41.0	33.0	41.0
16	37.98620662185244	40.0	37.0	41.0	33.0	41.0
17	37.97290527859718	40.0	37.0	41.0	33.0	41.0
18	37.94228665015616	40.0	37.0	41.0	33.0	41.0
19	37.9945688719983	40.0	37.0	41.0	33.0	41.0
20	37.92386580099766	40.0	36.0	41.0	33.0	41.0
21	37.750969425889004	40.0	36.0	41.0	33.0	41.0
22	37.871204009381465	40.0	36.0	41.0	33.0	41.0
23	37.84361959610965	40.0	36.0	41.0	33.0	41.0
24	37.780205670584984	40.0	36.0	41.0	33.0	41.0
25	37.69601381446536	40.0	36.0	41.0	33.0	41.0
26	37.625308400441426	39.0	36.0	41.0	33.0	41.0
27	37.530291776691136	39.0	36.0	41.0	33.0	41.0
28	37.3985318616023	39.0	35.0	41.0	32.0	41.0
29	37.270487981462	39.0	35.0	41.0	32.0	41.0
30	37.23806053903594	39.0	35.0	41.0	32.0	41.0
31	37.14882415375856	39.0	35.0	41.0	32.0	41.0
32	37.0311270644964	39.0	35.0	41.0	31.0	41.0
33	36.97024829017875	39.0	35.0	41.0	31.0	41.0
34	36.849905927614635	39.0	35.0	41.0	31.0	41.0
35	36.786405310228	39.0	35.0	41.0	31.0	41.0
36	36.61304033046	39.0	35.0	41.0	30.0	41.0
37	36.57594558562696	39.0	35.0	41.0	30.0	41.0
38	36.47517449666002	39.0	35.0	40.0	30.0	41.0
39	36.3734052797687	39.0	35.0	40.0	30.0	41.0
40	36.24226626585348	38.0	35.0	40.0	30.0	41.0
41	36.17206225881504	38.0	35.0	40.0	30.0	41.0
42	36.13699422913362	38.0	35.0	40.0	30.0	41.0
43	36.06455732089344	38.0	35.0	40.0	30.0	41.0
44	35.881290724673676	38.0	35.0	40.0	29.0	41.0
45	35.91255837056788	38.0	35.0	40.0	30.0	41.0
46	35.80739411291967	38.0	35.0	40.0	29.0	41.0
47	35.596332231331374	38.0	34.0	40.0	28.0	41.0
48	35.49342196209457	38.0	34.0	40.0	28.0	41.0
49	35.4695418686548	38.0	34.0	40.0	28.0	41.0
50	35.35799755856973	37.0	34.0	40.0	28.0	41.0
51	34.405324286139376	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	17.0
11	9.0
12	6.0
13	8.0
14	10.0
15	20.0
16	38.0
17	68.0
18	98.0
19	211.0
20	342.0
21	505.0
22	854.0
23	1159.0
24	1695.0
25	2305.0
26	3244.0
27	4037.0
28	4830.0
29	6041.0
30	7595.0
31	9640.0
32	12894.0
33	18189.0
34	29611.0
35	33541.0
36	38486.0
37	56394.0
38	90331.0
39	104558.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.15680683413035	4.7680524087451	54.879697468831935	15.195443288292616
2	20.192409073123414	4.7598518272067185	52.36305614586726	22.68468295380261
3	22.19241375917001	4.548276823516456	50.759491001619025	22.49981841569451
4	19.705763134402847	4.941201830369799	49.48863516549945	25.864399869727905
5	18.082750896792167	5.785393124163834	50.82228402597006	25.309571953073927
6	21.669685261680556	6.22517859695079	52.82369452599467	19.281441615373982
7	82.95145958636266	2.3099866681974417	9.697773424961165	5.040780320478726
8	84.71528752410386	2.805536095445397	7.412622803708538	5.066553576742214
9	79.2190234747504	5.365991954057999	9.970501336694792	5.444483234496801
10	39.512744875222296	29.934699940721508	16.47028226401655	14.082272920039642
11	28.434462123856896	25.16828764828409	26.922040585849544	19.47520964200947
12	25.711400448454658	22.540821323386417	31.650495900880742	20.09728232727818
13	25.3325335813814	23.306287034411984	31.62683136558427	19.73434801862235
14	20.585334079976757	26.151654525900952	32.00827555828388	21.254735835838414
15	18.14320089784653	25.7852056823001	33.8290389621344	22.242554457718974
16	22.740681210593277	26.022553942253847	29.120967949784326	22.11579689736855
17	23.70952134377072	24.96702194709922	27.65892141265561	23.664535296474455
18	24.184217863678217	24.28098472583113	30.163613316807208	21.371184093683443
19	24.37517426235769	25.152823694525996	28.366045843593824	22.105956199522492
20	24.9693649703959	27.54926792237095	27.98788188350957	19.49348522372358
21	24.649542290398994	26.818244653806595	30.053022617203883	18.479190438590532
22	24.385014960203748	23.439605059993113	29.615580167713606	22.55979981208953
23	22.28660329569657	25.73084754181711	28.64838015084384	23.334169011642484
24	22.529106206903016	26.21819638752668	28.22007549221062	23.032621913359684
25	22.601974231429782	27.740224321050423	26.105731269286007	23.552070178233784
26	21.53004107319839	28.045520256607908	26.112994641505722	24.31144402868798
27	21.003798040763918	27.670870831468676	29.440087722792228	21.885243404975174
28	18.370942762283885	28.221715608518295	28.071527815201065	25.335813813996754
29	20.13149046740972	28.04083421001455	27.636428389007474	24.191246933568262
30	22.750756210769005	26.22733417838374	27.66571618021598	23.35619343063128
31	23.600336458145403	28.202502817485513	25.25357369628326	22.943587028085823
32	23.099163774985414	27.471713851250822	24.66102310455273	24.76809926921103
33	23.415940524696637	26.81847895613626	25.24560741707455	24.519973102092553
34	20.885475364281547	26.77771035077402	27.667824901182993	24.668989383761442
35	20.500048031977585	28.26693595814423	25.713274867092	25.519741142786184
36	22.959988191162584	28.75381619919447	26.08651847825323	22.199677131389716
37	21.712562588009813	28.95086445844531	26.641580697236872	22.694992256308005
38	21.04995559970853	29.624717958570663	24.754744036419954	24.570582405300854
39	21.655158517241137	26.094484757461945	26.297156272624818	25.9532004526721
40	24.5893265916743	25.291764976019156	26.16360394471402	23.95530448759252
41	20.897190480764948	26.35760627367918	25.512712072896143	27.232491172659728
42	21.4984102586932	27.041300471650594	26.608544068753677	24.851745200902535
43	21.188193974212684	26.632677208709488	27.532163852305185	24.646964964772643
44	22.04925503574282	25.757323705069602	26.607372557105336	25.58604870208224
45	22.084634687522698	24.353852750357895	25.841672543750104	27.7198400183693
46	21.54480211996748	25.377753931007334	27.126586519649763	25.950857429375418
47	19.247936382231448	27.193362683605166	29.12518539171835	24.43351554244504
48	19.653982319546202	27.22850803305537	28.0434115356409	25.074098111757525
49	21.620247470120596	25.829020217948027	28.491866194625576	24.058866117305804
50	20.897190480764948	25.23318939360214	27.160794659781303	26.708825465851604
51	20.35923233184708	25.79738940344284	25.334642302348414	28.508735962361676
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	206.5
2	358.0
3	841.0
4	1324.0
5	950.5
6	577.0
7	635.0
8	693.0
9	830.0
10	967.0
11	1131.5
12	1296.0
13	1306.0
14	1316.0
15	1364.0
16	1412.0
17	1376.0
18	1340.0
19	1374.0
20	1408.0
21	1496.5
22	1585.0
23	1794.0
24	2003.0
25	2241.0
26	3090.0
27	3701.0
28	4492.0
29	5283.0
30	6008.5
31	6734.0
32	7820.0
33	8906.0
34	10000.0
35	11094.0
36	12495.5
37	13897.0
38	14924.0
39	15951.0
40	17763.5
41	19576.0
42	21459.0
43	23342.0
44	26299.0
45	29256.0
46	32972.5
47	36689.0
48	40803.0
49	44917.0
50	42770.5
51	40624.0
52	35162.0
53	29700.0
54	27352.5
55	25005.0
56	23522.0
57	22039.0
58	20373.0
59	18707.0
60	16946.0
61	15185.0
62	13410.5
63	11636.0
64	10605.0
65	9574.0
66	8100.5
67	6627.0
68	5557.5
69	4488.0
70	3579.0
71	2670.0
72	2232.0
73	1794.0
74	1527.0
75	1003.0
76	746.0
77	528.5
78	311.0
79	238.5
80	166.0
81	111.5
82	57.0
83	48.0
84	39.0
85	24.5
86	10.0
87	5.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	426799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.43042815384826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64986075337302	26.448765281171603
2	8.549208721030071	6.0580425072541795
3	3.133727341477692	3.330879042779259
4	1.772373262599716	2.5118377416936353
5	1.3204319430829778	2.3391734545723857
6	1.035839196878519	2.2020135746366547
7	0.8773387592264114	2.1759141512750424
8	0.7606131733230137	2.1559080312233263
9	0.7041137425745424	2.2452346231582085
>10	7.053060772305583	43.416669515245886
>50	0.1014033245931448	2.461042796492759
>100	0.03669199245146688	2.3976967083771177
>500	0.0026685085419248635	0.6261335351430387
>1k	0.0026685085419248635	1.630689036976906
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	2202	0.5159337299290767	No Hit
CTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGCT	1922	0.4503290776220188	No Hit
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	1699	0.39807965810604057	No Hit
CCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	1076	0.2521093067228367	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	748	0.1752581425917118	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	724	0.16963488667967827	No Hit
TCCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	668	0.1565139562182667	No Hit
CGGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCT	509	0.11925988580104452	No Hit
TGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	461	0.10801337397697745	No Hit
TTCCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCT	454	0.10637325766930102	No Hit
GCCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	443	0.10379593204295229	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.029069890041917E-4	0.0	0.0	0.5016412878193248	0.0
2	7.029069890041917E-4	0.0	0.0	1.8329471249932638	0.0
3	7.029069890041917E-4	0.0	0.0	2.5838860915794086	0.0
4	7.029069890041917E-4	0.0	0.0	3.5745163414159826	0.0
5	7.029069890041917E-4	0.0	0.0	4.9299553185457325	0.0
6	7.029069890041917E-4	0.0	0.0	5.671756494274822	0.0
7	7.029069890041917E-4	0.0	0.0	6.338112319850796	0.0
8	7.029069890041917E-4	0.0	0.0	7.251891405556245	0.0
9	7.029069890041917E-4	0.0	0.0	7.733616995353785	0.0
10	7.029069890041917E-4	0.0	0.0	8.645053057762553	0.0
11	7.029069890041917E-4	0.0	0.0	10.27204843497759	0.0
12	7.029069890041917E-4	0.0	0.0	11.6153036909646	0.0
13	7.029069890041917E-4	0.0	0.0	12.11132172287189	0.0
14	7.029069890041917E-4	0.0	0.0	12.276739167617544	0.0
15	7.029069890041917E-4	0.0	0.0	12.53728335820843	0.0
16	7.029069890041917E-4	0.0	0.0	13.15701302018046	0.0
17	7.029069890041917E-4	0.0	0.0	14.153266525929068	0.0
18	7.029069890041917E-4	0.0	0.0	15.291975848115857	0.0
19	7.029069890041917E-4	0.0	0.0	15.907956672813198	0.0
20	7.029069890041917E-4	0.0	0.0	16.565643312191455	0.0
21	7.029069890041917E-4	0.0	0.0	17.333920651173035	0.0
22	7.029069890041917E-4	0.0	0.0	18.077127640880132	0.0
23	7.029069890041917E-4	0.0	0.0	18.754729978280174	0.0
24	7.029069890041917E-4	0.0	0.0	19.300420104077094	0.0
25	7.029069890041917E-4	0.0	0.0	19.754498018973802	0.0
26	7.029069890041917E-4	0.0	0.0	20.18092825896968	0.0
27	7.029069890041917E-4	0.0	0.0	20.665699779052904	0.0
28	7.029069890041917E-4	0.0	0.0	21.078306181598364	0.0
29	7.029069890041917E-4	0.0	0.0	21.598223051131797	0.0
30	7.029069890041917E-4	0.0	0.0	22.139930037324362	0.0
31	9.372093186722555E-4	0.0	0.0	22.580418417100322	0.0
32	0.0011715116483403194	0.0	0.0	22.994196331294123	0.0
33	0.0011715116483403194	0.0	0.0	23.41687773401531	0.0
34	0.0011715116483403194	0.0	0.0	23.862520765043968	0.0
35	0.0011715116483403194	0.0	0.0	24.358773099280928	0.0
36	0.0011715116483403194	0.0	0.0	24.7962155487712	0.0
37	0.0011715116483403194	0.0	0.0	25.203901602393632	0.0
38	0.0011715116483403194	0.0	0.0	25.651419052059634	0.0
39	0.0011715116483403194	0.0	0.0	26.279817900229382	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTCT	20	7.0282974E-4	45.0	32
GCACGAA	25	3.8866696E-5	45.0	34
TATTGCG	25	3.8866696E-5	45.0	1
TACGCAA	20	7.0282974E-4	45.0	24
CGGGCTA	25	3.8866696E-5	45.0	6
GCGATAG	25	3.8866696E-5	45.0	9
TTACGGG	45	3.8380676E-10	45.0	3
TTACGCA	20	7.0282974E-4	45.0	23
CGCAAAT	20	7.0282974E-4	45.0	26
TACGTTC	20	7.0282974E-4	45.0	31
AATCGTA	25	3.8866696E-5	45.0	12
AGTCGTG	20	7.0282974E-4	45.0	21
CTAATAC	25	3.8866696E-5	45.0	32
AGTCGGC	25	3.8866696E-5	45.0	30
CTTACGC	20	7.0282974E-4	45.0	22
TCTTGCG	120	0.0	43.125004	1
GCTACGA	55	6.002665E-11	40.909092	10
ACACGCG	265	0.0	40.75472	36
TACACGC	265	0.0	40.75472	35
TCGTTGA	50	1.0786607E-9	40.5	24
>>END_MODULE