##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934415.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3255111
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90598231519601	33.0	31.0	34.0	30.0	34.0
2	32.1181535744864	33.0	31.0	34.0	30.0	34.0
3	32.12312514074021	33.0	31.0	34.0	30.0	34.0
4	35.759167045301986	37.0	35.0	37.0	35.0	37.0
5	35.75385447685194	37.0	35.0	37.0	35.0	37.0
6	35.46411689186636	37.0	35.0	37.0	33.0	37.0
7	35.830326216218126	37.0	35.0	37.0	35.0	37.0
8	35.92574385328181	37.0	35.0	37.0	35.0	37.0
9	37.70532372014349	39.0	38.0	39.0	35.0	39.0
10	37.03149600735582	39.0	37.0	39.0	33.0	39.0
11	36.93676037468461	39.0	37.0	39.0	33.0	39.0
12	36.87787390353201	39.0	37.0	39.0	33.0	39.0
13	36.87187564417926	39.0	37.0	39.0	33.0	39.0
14	37.9599144238092	40.0	37.0	41.0	33.0	41.0
15	37.949285293189696	40.0	37.0	41.0	33.0	41.0
16	37.95895316626683	40.0	37.0	41.0	33.0	41.0
17	37.89150600394272	40.0	37.0	41.0	33.0	41.0
18	37.775257126408285	39.0	37.0	41.0	33.0	41.0
19	37.72276859375917	39.0	37.0	41.0	33.0	41.0
20	37.56630849147694	39.0	36.0	41.0	33.0	41.0
21	37.40042044649169	39.0	35.0	40.0	32.0	41.0
22	37.51395543807876	39.0	35.0	41.0	33.0	41.0
23	37.449680517807224	39.0	35.0	40.0	33.0	41.0
24	37.36670024463068	39.0	35.0	41.0	32.0	41.0
25	37.29201339063399	39.0	35.0	40.0	32.0	41.0
26	37.22645279991988	39.0	35.0	40.0	32.0	41.0
27	37.15185104286766	39.0	35.0	40.0	32.0	41.0
28	37.04471460420244	39.0	35.0	40.0	32.0	41.0
29	36.964069428047154	39.0	35.0	40.0	31.0	41.0
30	36.882266687679774	39.0	35.0	40.0	31.0	41.0
31	36.76425903755663	39.0	35.0	40.0	31.0	41.0
32	36.517213391494174	39.0	35.0	40.0	30.0	41.0
33	36.266488915431765	39.0	35.0	40.0	30.0	41.0
34	35.99917330008101	39.0	35.0	40.0	29.0	41.0
35	35.84658157586638	39.0	35.0	40.0	27.0	41.0
36	35.60409030598342	39.0	35.0	40.0	26.0	41.0
37	35.54512273160577	39.0	35.0	40.0	26.0	41.0
38	35.48859071165315	38.0	35.0	40.0	25.0	41.0
39	35.397492435741825	38.0	35.0	40.0	25.0	41.0
40	35.302364189731165	38.0	35.0	40.0	25.0	41.0
41	35.21857995011537	38.0	35.0	40.0	24.0	41.0
42	35.09890353969496	38.0	34.0	40.0	24.0	41.0
43	34.97985967298811	38.0	34.0	40.0	23.0	41.0
44	34.84781102702796	38.0	34.0	40.0	23.0	41.0
45	34.925314374840056	38.0	34.0	40.0	23.0	41.0
46	34.807678447831734	38.0	34.0	40.0	23.0	41.0
47	34.58606787909844	38.0	34.0	40.0	23.0	41.0
48	34.48115471331085	38.0	33.0	40.0	22.0	41.0
49	34.50243785849392	38.0	33.0	40.0	22.0	41.0
50	34.3965367079648	38.0	33.0	40.0	22.0	41.0
51	33.441309681912536	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	16.0
10	20.0
11	18.0
12	23.0
13	31.0
14	29.0
15	63.0
16	147.0
17	350.0
18	824.0
19	1728.0
20	3182.0
21	5214.0
22	8630.0
23	13384.0
24	22869.0
25	37814.0
26	53561.0
27	58124.0
28	57626.0
29	59680.0
30	68671.0
31	82487.0
32	102733.0
33	134655.0
34	194595.0
35	238936.0
36	295484.0
37	428091.0
38	707844.0
39	678157.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.56734440085146	4.042750001459243	57.739352052817864	16.650553544871435
2	24.89359041826838	3.8187637840921553	51.40786289622689	19.87978290141258
3	17.49700701450734	3.8355374056368587	57.42636733432439	21.24108824553141
4	16.514644201073327	4.102041374318725	56.348677510536504	23.03463691407144
5	16.18334981510615	4.780328535647479	56.403760117550526	22.632561531695846
6	18.82052562877272	5.3479896691694995	58.441509367883306	17.389975334174473
7	77.07595839281672	1.4072638383145768	16.46321738337034	5.053560385498375
8	79.3219647502036	1.626887685243299	13.81243220277281	5.23871536178029
9	74.5169058750992	4.012582059413642	16.15413422153653	5.316377843950637
10	33.16310872348132	29.574260294042197	22.921983305638424	14.340647676838056
11	21.387504143483895	24.985445964822706	33.720017535500325	19.907032356193078
12	19.163094591858773	21.77597015892853	38.62246172250347	20.438473526709227
13	20.83317588862561	21.709490091121317	39.31813692374853	18.139197096504542
14	18.480998036626094	24.10329478779679	37.84651276100877	19.56919441456835
15	16.452004248088624	25.705575017257477	38.350151500209975	19.492269234443928
16	18.51617348840024	25.92633553817366	35.39645806241323	20.161032911012867
17	19.08260578517906	25.97727082117937	33.57218847529316	21.367934918348404
18	19.034681152194196	24.662292622279242	36.10248621321976	20.2005400123068
19	18.888603184346096	27.368314014483687	34.46039167327935	19.282691127890878
20	19.607626283711983	27.985927361616852	34.18927956681047	18.217166787860688
21	19.93928932070212	27.778131068341448	34.543030944259655	17.739548666696773
22	18.785810990777275	25.151615413422153	33.79580604163729	22.266767554163284
23	18.381646585938235	26.041569703767397	32.70524415296437	22.871539557329996
24	18.959015529731552	25.6818277471951	33.095983516383924	22.26317320668942
25	17.946361890577617	27.00814196505127	31.153161904463474	23.89233423990764
26	17.091982423948064	27.301373132897773	32.37868078845852	23.227963654695646
27	17.472768209747684	26.658814399877606	33.958719072867254	21.909698317507452
28	15.43280705327714	25.350502640309347	35.152871898992075	24.063818407421437
29	16.1886645340205	23.958722144959115	34.837091576907824	25.01552174411257
30	18.20168344489635	25.68600579212199	33.772949678213735	22.339361084767923
31	16.838258357395492	27.339467071937023	33.11644364815823	22.70583092250925
32	16.431697720907213	27.150533422669763	31.682698378027663	24.73507047839536
33	18.00777300681912	25.8973349910341	31.504578492100578	24.5903135100462
34	16.208479526504625	24.130759289007347	33.35305616306172	26.307705021426305
35	15.532895806010917	24.53836443672735	31.56522158537758	28.363518171884156
36	17.203007823696336	26.9431365013359	30.623594709980708	25.23026096498706
37	16.415722843245593	26.85659567369592	34.63900309390371	22.08867838915478
38	16.6850838573554	25.311579236468436	34.34801455311355	23.655322353062612
39	18.379311796126157	23.300403580707385	33.362979019763074	24.957305603403388
40	18.624218959046253	23.152175148558683	32.59010215012637	25.633503742268697
41	17.858346458845798	24.182093943954598	30.06834482756502	27.89121476963458
42	20.494938575059347	24.792149945117078	29.132493484861193	25.580417994962385
43	19.82875545565113	24.079701122327318	31.552902497026984	24.53864092499457
44	18.002489008823357	23.796484973937908	30.67975254914502	27.521273468093714
45	18.992317005472316	22.45935699274157	30.16182858280409	28.386497418982024
46	18.822338162968943	23.538982234399995	31.621563750053376	26.017115852577682
47	15.882684184963278	24.467675603074674	33.56417031554378	26.085469896418278
48	16.3505944958559	23.62610675949299	33.93954921967331	26.083749524977794
49	19.26118648488485	21.18824826557374	34.19487077399204	25.355694475549374
50	18.015146027278334	20.47552295451676	32.7556879012728	28.75364311693211
51	16.818627690422847	20.02626024120222	31.20901253444199	31.94609953393294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	442.0
1	1181.0
2	1920.0
3	28933.0
4	55946.0
5	37612.5
6	19279.0
7	19484.5
8	19690.0
9	20785.5
10	21881.0
11	22847.5
12	23814.0
13	24257.5
14	24701.0
15	23920.5
16	23140.0
17	22453.5
18	21767.0
19	20796.0
20	19825.0
21	19765.5
22	19706.0
23	20456.5
24	21207.0
25	22570.0
26	26714.5
27	29496.0
28	33509.5
29	37523.0
30	41424.5
31	45326.0
32	53794.5
33	62263.0
34	73260.5
35	84258.0
36	95681.5
37	107105.0
38	120414.0
39	133723.0
40	151119.0
41	168515.0
42	192814.0
43	217113.0
44	247208.0
45	277303.0
46	328125.5
47	378948.0
48	411492.0
49	444036.0
50	400402.0
51	356768.0
52	288563.5
53	220359.0
54	177711.5
55	135064.0
56	110892.0
57	86720.0
58	74180.5
59	61641.0
60	52360.5
61	43080.0
62	35924.5
63	28769.0
64	23457.5
65	18146.0
66	14131.0
67	10116.0
68	7531.5
69	4947.0
70	3941.5
71	2936.0
72	2202.5
73	1469.0
74	1281.5
75	880.0
76	666.0
77	476.5
78	287.0
79	201.0
80	115.0
81	78.0
82	41.0
83	24.5
84	8.0
85	11.0
86	14.0
87	9.5
88	5.0
89	4.0
90	3.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3255111.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.37931197494592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28169370299915	25.931789733239448
2	12.272961408463543	8.929637838699339
3	5.263788700038532	5.7447903386669035
4	2.761609607176327	4.0186182980990175
5	1.6325969504010753	2.9696376893993013
6	1.0974211198331867	2.395405517178361
7	0.7729625449965577	1.9683891898564565
8	0.5791084434698337	1.6854053385851533
9	0.4254570815503042	1.3930052311501673
>10	3.609011570671218	26.357230345229333
>50	0.2105705223422346	5.045063571282636
>100	0.08260781319849658	5.727842217244868
>500	0.005700881963379297	1.4322269477493543
>1k	0.003828950572338735	2.9981766993645955
>5k	2.5526337148924896E-4	0.566774198985791
>10k+	4.2543895248208164E-4	2.8360068452692073
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38364	1.1785773204047418	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	15615	0.47970714362736017	No Hit
TCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	15075	0.4631178475941374	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	11649	0.3578679805389125	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	10916	0.33534954721974153	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	8059	0.2475798828365607	No Hit
TTCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	5146	0.15808984701289755	No Hit
TCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	5105	0.15683028935111581	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	4638	0.1424836203742361	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	4432	0.1361551111467474	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	4248	0.1305024621280196	No Hit
CGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	4191	0.12875136976895718	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT	4169	0.12807550956019625	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTC	4093	0.12574071974811304	No Hit
CGTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	3918	0.12036455899660564	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG	3910	0.12011879164796531	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGT	3449	0.10595644818256582	No Hit
TGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	3330	0.1023006588715408	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3793010438046506E-4	0.0	0.0	0.5405345624158439	0.0
2	3.3793010438046506E-4	0.0	0.0	1.747713058018605	0.0
3	3.3793010438046506E-4	0.0	0.0	2.6068542670280674	0.0
4	3.3793010438046506E-4	0.0	0.0	3.860513512442433	0.0
5	3.3793010438046506E-4	0.0	0.0	5.753413631670318	0.0
6	3.3793010438046506E-4	0.0	0.0	7.290012537206873	0.0
7	3.6865102296050736E-4	0.0	0.0	8.640872769008492	0.0
8	3.6865102296050736E-4	0.0	0.0	10.596720050406883	0.0
9	3.6865102296050736E-4	0.0	0.0	11.638712166804757	0.0
10	3.993719415405496E-4	0.0	0.0	13.425010698559895	0.0
11	3.993719415405496E-4	0.0	0.0	16.715958380528345	0.0
12	3.993719415405496E-4	0.0	0.0	19.296761308600537	0.0
13	3.993719415405496E-4	0.0	0.0	20.18662343619004	0.0
14	3.993719415405496E-4	0.0	0.0	20.49106773931826	0.0
15	3.993719415405496E-4	0.0	0.0	20.870962618479062	0.0
16	3.993719415405496E-4	0.0	0.0	21.76294448945059	0.0
17	3.993719415405496E-4	0.0	0.0	23.206121081585238	0.0
18	3.993719415405496E-4	0.0	0.0	24.790030201735057	0.0
19	3.993719415405496E-4	0.0	0.0	25.98581123654462	0.0
20	3.993719415405496E-4	0.0	0.0	27.197413544422908	0.0
21	4.300928601205919E-4	0.0	0.0	28.872502350918296	0.0
22	4.300928601205919E-4	0.0	0.0	30.653424721921926	0.0
23	4.300928601205919E-4	0.0	0.0	32.369003699105804	0.0
24	4.300928601205919E-4	0.0	0.0	33.63587293950959	0.0
25	4.300928601205919E-4	0.0	0.0	34.72548247970653	0.0
26	4.300928601205919E-4	0.0	0.0	35.697523064497645	0.0
27	4.300928601205919E-4	0.0	0.0	36.64925712210736	0.0
28	4.6081377870063416E-4	0.0	0.0	37.64025251366236	0.0
29	4.6081377870063416E-4	0.0	0.0	38.673704214694986	0.0
30	4.6081377870063416E-4	0.0	0.0	39.79440332449492	0.0
31	4.6081377870063416E-4	0.0	0.0	40.824721491832385	0.0
32	4.6081377870063416E-4	0.0	0.0	41.81043288539162	0.0
33	4.915346972806764E-4	0.0	0.0	42.764471011894834	0.0
34	4.915346972806764E-4	0.0	0.0	43.70004586633144	0.0
35	4.915346972806764E-4	0.0	0.0	44.67270086949416	0.0
36	5.222556158607188E-4	0.0	0.0	45.60867509587231	0.0
37	5.222556158607188E-4	0.0	0.0	46.52821363081013	0.0
38	5.222556158607188E-4	0.0	0.0	47.43908886670839	0.0
39	5.222556158607188E-4	0.0	0.0	48.42842532866007	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGA	25	3.8923863E-5	45.0	16
CGCACGA	20	7.0351944E-4	45.0	42
CTAGCGA	25	3.8923863E-5	45.0	35
TAGCGCG	30	2.1667329E-6	44.999996	1
CGTTTTT	25315	0.0	44.395615	1
TATCGCG	95	0.0	40.26316	1
TGGGCGA	4355	0.0	39.47187	6
GTCCGCG	80	0.0	39.375	1
TTACGCG	80	0.0	39.375	1
TACGCGG	120	0.0	39.374996	2
TTCTTCG	705	0.0	39.25532	1
TCTTGCG	975	0.0	39.000004	1
GCCGATA	75	0.0	39.0	9
GTTTTTT	28790	0.0	38.880688	2
GCGCGAC	215	0.0	38.720932	9
CGAATAC	35	6.2518193E-6	38.571426	12
TTCCGCG	355	0.0	38.02817	1
TTGGGCG	3665	0.0	38.001366	5
AGGCACG	370	0.0	37.7027	10
TACGGGA	430	0.0	37.67442	4
>>END_MODULE