FastQCFastQC Report
Sat 14 Jan 2017
SRR2934414.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934414.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220697
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT496072.2338482017132457No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC422511.9026008500934617No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG368901.6611901578648507No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC346561.560591111709522No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC107500.48408224985218606TruSeq Adapter, Index 19 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT97040.4369799211688943No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT95180.4286041724737774No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC89690.40388220455109364No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT79810.3593916684716555No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT76700.34538705640616435No Hit
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT76300.3435858201276446TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGTTCACT74780.33674112226926955No Hit
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC67010.30175210755902315TruSeq Adapter, Index 19 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC66340.2987350367925025No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTA60430.27212177077737304No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC60340.2717164926147061No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC55190.24852557552876414TruSeq Adapter, Index 19 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT43630.19646984707954304No Hit
CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG43480.19579438347509812No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT40620.18291554408368182No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC39330.17710655708545558No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGT37020.16670441757700397No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG28120.12662691037993926No Hit
TCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG27440.12356480870645567No Hit
TTCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT27080.12194369605578789No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC26450.11910674891711925No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG25800.11617973996452466No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC24690.11118130929163232No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCAC207.03471E-445.033
CGTTTTT248100.044.4558681
TTCGTCG1350.043.3333321
CTACGGC5750.041.478266
CGTTTTC10000.041.3999981
CCGATGA11100.041.35135318
TACGGCT45700.041.0120357
CGATGAA11300.040.6194719
CTATGCG501.0822987E-940.51
ACGGCTG46650.040.273318
CGGCGAA1350.040.031
CGAACGA451.9297659E-840.022
ATGCTAC9700.039.8969083
GTTTTTT274700.039.831642
CGGGATA6500.039.461546
TCGATCA1200.039.37499617
CGTTTCT11100.039.3243261
TGGGCGA27050.039.177456
TATTGCG1750.038.571431
ACGGCGG1050.038.5714330
Produced by FastQC (version 0.11.5)