##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934414.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2220697 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.856878268399516 33.0 31.0 34.0 30.0 34.0 2 32.06655342894596 33.0 31.0 34.0 30.0 34.0 3 32.04409246286188 33.0 31.0 34.0 30.0 34.0 4 35.76556729711437 37.0 35.0 37.0 35.0 37.0 5 35.75019869887697 37.0 35.0 37.0 35.0 37.0 6 35.22552649010648 37.0 35.0 37.0 32.0 37.0 7 35.742520478930714 37.0 35.0 37.0 35.0 37.0 8 35.68528124278098 37.0 35.0 37.0 35.0 37.0 9 37.577358820226266 39.0 37.0 39.0 35.0 39.0 10 37.10837183100621 39.0 37.0 39.0 33.0 39.0 11 37.002107896754936 39.0 37.0 39.0 33.0 39.0 12 36.95733231503442 39.0 37.0 39.0 33.0 39.0 13 36.86259539234754 39.0 37.0 39.0 33.0 39.0 14 37.92446380573306 40.0 37.0 41.0 33.0 41.0 15 37.879401377135196 40.0 37.0 41.0 33.0 41.0 16 38.014866503624766 40.0 37.0 41.0 33.0 41.0 17 37.86834989194834 40.0 37.0 41.0 33.0 41.0 18 37.70870451934685 39.0 37.0 41.0 33.0 41.0 19 37.587987915505806 39.0 37.0 41.0 33.0 41.0 20 37.27275490532927 39.0 35.0 40.0 32.0 41.0 21 37.21415258362577 39.0 35.0 40.0 32.0 41.0 22 37.37604499848471 39.0 35.0 40.0 33.0 41.0 23 37.263608677816016 39.0 35.0 40.0 33.0 41.0 24 37.172979474462295 39.0 35.0 41.0 32.0 41.0 25 37.121870745986506 39.0 35.0 40.0 32.0 41.0 26 37.09779046848805 39.0 35.0 40.0 32.0 41.0 27 36.94957213883749 39.0 35.0 40.0 32.0 41.0 28 36.80009519533732 39.0 35.0 40.0 31.0 41.0 29 36.62396761016924 38.0 35.0 40.0 31.0 41.0 30 36.475662821177316 38.0 35.0 40.0 30.0 41.0 31 36.29930557838372 38.0 35.0 40.0 30.0 41.0 32 35.71807905355841 38.0 35.0 40.0 28.0 41.0 33 35.425409229624755 38.0 35.0 40.0 25.0 41.0 34 35.05590316914014 38.0 34.0 40.0 24.0 41.0 35 34.710177030004544 38.0 34.0 40.0 23.0 41.0 36 34.60698150175373 38.0 34.0 40.0 22.0 41.0 37 34.35713922250537 38.0 33.0 40.0 21.0 41.0 38 34.343050402643854 38.0 33.0 40.0 20.0 41.0 39 34.19504056609254 38.0 33.0 40.0 19.0 41.0 40 34.14396831265139 38.0 33.0 40.0 19.0 41.0 41 34.111937378219544 38.0 33.0 40.0 18.0 41.0 42 34.08453111793279 38.0 33.0 40.0 18.0 41.0 43 34.0903581172938 38.0 33.0 40.0 18.0 41.0 44 33.962421257830314 38.0 33.0 40.0 18.0 41.0 45 34.02678348284345 38.0 33.0 40.0 19.0 41.0 46 33.841264702028234 38.0 33.0 40.0 18.0 41.0 47 33.52442634001847 37.0 32.0 40.0 18.0 41.0 48 33.42700422434938 37.0 32.0 40.0 17.0 41.0 49 33.48244672731129 37.0 32.0 40.0 18.0 41.0 50 33.36105871264743 37.0 32.0 40.0 17.0 41.0 51 32.51811300686226 36.0 31.0 39.0 14.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 11.0 10 15.0 11 11.0 12 9.0 13 21.0 14 22.0 15 56.0 16 107.0 17 297.0 18 733.0 19 1597.0 20 3188.0 21 5524.0 22 9235.0 23 14017.0 24 21566.0 25 35545.0 26 49129.0 27 50446.0 28 47309.0 29 46887.0 30 52302.0 31 62327.0 32 76776.0 33 99016.0 34 137601.0 35 168566.0 36 205288.0 37 285089.0 38 439018.0 39 408874.0 40 114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.325339746935306 3.634354439169324 48.879923735655964 20.1603820782394 2 26.666852794415448 12.964263021925099 42.7803973257045 17.58848685795496 3 14.753611141006632 12.96354252741369 54.106841230478544 18.176005101101143 4 14.186401836900755 3.4330662850447404 62.23149758836978 20.149034289684725 5 20.952926040788096 4.090832743053195 53.697285131650105 21.2589560845086 6 16.71668849915139 10.979435735717209 56.546750862454445 15.757124902676953 7 67.69906025000259 1.082272817948599 26.151834311479682 5.066832620569127 8 67.94281254939327 7.134066466519295 19.30353398054755 5.61958700353988 9 63.929387935409466 3.4134778405158386 20.032944611534127 12.624189612540567 10 33.112666878912336 25.558822297684014 27.170883736052236 14.157627087351404 11 21.61461018770233 20.964679107505436 37.96218034247806 19.458530362314175 12 18.19100039311982 18.105621793517983 41.278616578488645 22.424761234873554 13 19.399855090541394 19.528283237199854 44.865238256277195 16.206623415981557 14 17.021007368407307 24.717735017429213 39.058682927026965 19.20257468713652 15 13.459648029424995 23.37459815544399 44.849567500654075 18.31618631447694 16 15.809856094730618 25.64474126816941 36.2941905176618 22.251212119438176 17 16.37445360623264 26.104011488284982 37.707800749044104 19.81373415643827 18 16.540662683833048 24.660906012841913 37.780210447440595 21.018220855884437 19 16.10138618640904 27.439358003365612 35.01513263628491 21.444123173940437 20 16.878259393334616 28.156024887681657 37.93529689102115 17.030418827962574 21 16.940672230385324 30.009451987371534 36.496334259018674 16.553541523224467 22 15.971652143448656 23.075097593233114 37.96564772231421 22.98760254100402 23 15.7831077359946 27.60768353359328 36.39618552193298 20.21302320847914 24 18.057393692160613 25.090545896175843 34.635657183307764 22.216403228355784 25 16.72929715310103 30.775337652998136 32.03867974784493 20.4566854460559 26 17.27871924895652 25.32065383075674 34.62453454928791 22.776092370998835 27 19.72421271339584 26.456828644340042 33.059170161440306 20.75978848082381 28 17.24215415250257 24.788253417733262 35.751342934222905 22.218249495541265 29 21.499511189504915 22.707014959717604 33.85779329642901 21.935680554348476 30 20.445292626594263 27.85521842916886 34.07173513541019 17.62775380882669 31 22.851023800185256 24.742231830817083 30.982074546865242 21.42466982213242 32 21.269538347644907 28.007062647448077 32.50515491307459 18.218244091832428 33 18.832015353738036 24.3087643203913 32.78984030689463 24.069380018976023 34 21.127330743455772 23.9384751724346 31.980139568793042 22.95405451531659 35 17.05189857058392 28.88917308394617 30.62867198901966 23.43025635645025 36 21.66292835087362 26.625694545451275 28.809108131365964 22.902268972309145 37 16.81818816344598 28.51501127799065 33.7160360013095 20.95076455725387 38 19.38508495305753 26.043264794791902 32.157066002250644 22.414584249899917 39 18.512385976114707 23.660499383752036 33.80240528086452 24.024709359268734 40 17.958911098632548 25.953878444470362 33.71792729940194 22.369283157495147 41 17.50054149665623 26.90150885059961 28.100726933931103 27.49722271881306 42 18.376077420737722 27.257162953793333 32.340521917217885 22.02623770825106 43 20.380313027846665 24.645730597195385 30.150668911607482 24.82328746335047 44 20.419174700555725 24.39031529290128 30.80699438059312 24.38351562594987 45 19.838005815291325 22.551478207067422 30.12599197459176 27.484524003049494 46 23.346769054940857 24.668741390653476 29.485337261229244 22.499152293176422 47 16.81382016547057 24.234103076646658 36.33620435385827 22.615872404024504 48 18.871777644586363 25.930372311035683 30.217179561191827 24.980670483186135 49 18.7658649514094 22.280977548940715 35.69068630254375 23.262471197106134 50 18.827332139413887 21.426516089317904 33.017786757941316 26.7283650133269 51 17.407957951940315 21.802884409714608 30.67347774144784 30.115679896897234 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 307.0 1 790.5 2 1274.0 3 33743.0 4 66212.0 5 42489.5 6 18767.0 7 18804.5 8 18842.0 9 20329.0 10 21816.0 11 23081.5 12 24347.0 13 24423.0 14 24499.0 15 23714.5 16 22930.0 17 21757.0 18 20584.0 19 19451.5 20 18319.0 21 17913.5 22 17508.0 23 17457.0 24 17406.0 25 17885.5 26 20106.0 27 21847.0 28 24054.0 29 26261.0 30 29474.5 31 32688.0 32 36905.0 33 41122.0 34 47247.0 35 53372.0 36 58309.0 37 63246.0 38 69917.5 39 76589.0 40 85379.5 41 94170.0 42 107239.0 43 120308.0 44 137874.0 45 155440.0 46 264505.5 47 373571.0 48 324449.0 49 275327.0 50 243993.0 51 212659.0 52 172223.5 53 131788.0 54 110150.0 55 88512.0 56 74279.0 57 60046.0 58 50109.5 59 40173.0 60 32957.0 61 25741.0 62 20600.0 63 15459.0 64 12611.5 65 9764.0 66 7544.0 67 5324.0 68 4004.5 69 2685.0 70 1990.5 71 1296.0 72 1123.5 73 951.0 74 757.5 75 490.5 76 417.0 77 263.0 78 109.0 79 85.5 80 62.0 81 41.0 82 20.0 83 12.5 84 5.0 85 4.0 86 3.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2220697.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.297500721275114 #Duplication Level Percentage of deduplicated Percentage of total 1 75.99386303696136 27.58387298396586 2 10.605510182731454 7.69907027014371 3 3.993516801560335 4.348640369551816 4 2.022240142458366 2.9360905211789605 5 1.2573774257197567 2.2819829008488957 6 0.8721754875834143 1.899467423378247 7 0.6408747187303616 1.6283505395753601 8 0.493422281517243 1.432799649541227 9 0.4146017448528488 1.354410641955438 >10 3.4754465590184616 24.588714325201565 >50 0.14769207171990692 3.597429754938048 >100 0.07279238881872926 4.874899409372896 >500 0.005493273409785538 1.3915302450391207 >1k 0.00287148382784244 2.417629158590455 >5k 0.0014981654753960557 4.07308203192631 >10k+ 6.242356147483565E-4 7.8920297747921655 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49607 2.2338482017132457 No Hit GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC 42251 1.9026008500934617 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG 36890 1.6611901578648507 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC 34656 1.560591111709522 No Hit GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC 10750 0.48408224985218606 TruSeq Adapter, Index 19 (95% over 21bp) GAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT 9704 0.4369799211688943 No Hit GAATGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT 9518 0.4286041724737774 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC 8969 0.40388220455109364 No Hit CGTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT 7981 0.3593916684716555 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT 7670 0.34538705640616435 No Hit CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT 7630 0.3435858201276446 TruSeq Adapter, Index 13 (95% over 22bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGTTCACT 7478 0.33674112226926955 No Hit TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC 6701 0.30175210755902315 TruSeq Adapter, Index 19 (95% over 21bp) CGTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC 6634 0.2987350367925025 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTA 6043 0.27212177077737304 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC 6034 0.2717164926147061 No Hit CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC 5519 0.24852557552876414 TruSeq Adapter, Index 19 (95% over 21bp) CGTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT 4363 0.19646984707954304 No Hit CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG 4348 0.19579438347509812 No Hit GAATGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT 4062 0.18291554408368182 No Hit CGTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC 3933 0.17710655708545558 No Hit CGTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGT 3702 0.16670441757700397 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG 2812 0.12662691037993926 No Hit TCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG 2744 0.12356480870645567 No Hit TTCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT 2708 0.12194369605578789 No Hit GAATGCTACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC 2645 0.11910674891711925 No Hit GAATGCTACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG 2580 0.11617973996452466 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC 2469 0.11118130929163232 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.3509272088898215E-4 0.0 0.0 0.3756928567922594 0.0 2 1.3509272088898215E-4 0.0 0.0 1.5410927289945453 0.0 3 1.3509272088898215E-4 0.0 0.0 2.260911776798005 0.0 4 1.3509272088898215E-4 0.0 0.0 3.692489340058549 0.0 5 1.3509272088898215E-4 0.0 0.0 7.4242906618957925 0.0 6 1.3509272088898215E-4 0.0 0.0 9.42082598391406 0.0 7 1.3509272088898215E-4 0.0 0.0 11.503055121882904 0.0 8 1.3509272088898215E-4 0.0 0.0 13.815392194432649 0.0 9 1.3509272088898215E-4 0.0 0.0 14.944497155622761 0.0 10 1.3509272088898215E-4 0.0 0.0 18.193342000281895 0.0 11 1.3509272088898215E-4 0.0 0.0 20.438718114177664 0.0 12 1.3509272088898215E-4 0.0 0.0 23.97260859991255 0.0 13 1.3509272088898215E-4 0.0 0.0 24.74056568725945 0.0 14 1.3509272088898215E-4 0.0 0.0 25.111800484262375 0.0 15 1.3509272088898215E-4 0.0 0.0 25.71652053386842 0.0 16 1.3509272088898215E-4 0.0 0.0 26.397117661707114 0.0 17 1.3509272088898215E-4 0.0 0.0 27.327636323190422 0.0 18 1.3509272088898215E-4 0.0 0.0 28.28611917789775 0.0 19 1.3509272088898215E-4 0.0 0.0 29.457102882563447 0.0 20 1.3509272088898215E-4 0.0 0.0 30.194438953175514 0.0 21 1.3509272088898215E-4 0.0 0.0 31.13063150893616 0.0 22 1.3509272088898215E-4 0.0 0.0 32.21731735576713 0.0 23 1.3509272088898215E-4 0.0 0.0 33.2112845651613 0.0 24 1.3509272088898215E-4 0.0 0.0 33.96483176228004 0.0 25 2.2515453481497026E-4 0.0 0.0 34.614762842476935 0.0 26 2.701854417779643E-4 0.0 0.0 35.20781988717957 0.0 27 2.701854417779643E-4 0.0 0.0 35.847213735147115 0.0 28 3.1521634874095836E-4 0.0 0.0 36.46535299502814 0.0 29 3.1521634874095836E-4 0.0 0.0 37.142392681216755 0.0 30 3.1521634874095836E-4 0.0 0.0 37.88747406782645 0.0 31 3.6024725570395243E-4 0.0 0.0 38.542988980486754 0.0 32 3.6024725570395243E-4 0.0 0.0 39.16054283857726 0.0 33 3.6024725570395243E-4 0.0 0.0 39.758373159417964 0.0 34 3.6024725570395243E-4 0.0 0.0 40.37646738839202 0.0 35 3.6024725570395243E-4 0.0 0.0 41.02842485940225 0.0 36 3.6024725570395243E-4 0.0 0.0 41.63219925996208 0.0 37 3.6024725570395243E-4 0.0 0.0 42.22998454989582 0.0 38 3.6024725570395243E-4 0.0 0.0 42.86451505991137 0.0 39 4.0527816266694645E-4 0.0 0.0 43.71609454148855 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTCAC 20 7.03471E-4 45.0 33 CGTTTTT 24810 0.0 44.455868 1 TTCGTCG 135 0.0 43.333332 1 CTACGGC 575 0.0 41.47826 6 CGTTTTC 1000 0.0 41.399998 1 CCGATGA 1110 0.0 41.351353 18 TACGGCT 4570 0.0 41.012035 7 CGATGAA 1130 0.0 40.61947 19 CTATGCG 50 1.0822987E-9 40.5 1 ACGGCTG 4665 0.0 40.27331 8 CGGCGAA 135 0.0 40.0 31 CGAACGA 45 1.9297659E-8 40.0 22 ATGCTAC 970 0.0 39.896908 3 GTTTTTT 27470 0.0 39.83164 2 CGGGATA 650 0.0 39.46154 6 TCGATCA 120 0.0 39.374996 17 CGTTTCT 1110 0.0 39.324326 1 TGGGCGA 2705 0.0 39.17745 6 TATTGCG 175 0.0 38.57143 1 ACGGCGG 105 0.0 38.57143 30 >>END_MODULE