##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934413.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 508237 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.000354165477916 33.0 31.0 34.0 30.0 34.0 2 32.18501407807775 34.0 31.0 34.0 30.0 34.0 3 32.26644852696675 34.0 31.0 34.0 30.0 34.0 4 35.81551717013913 37.0 35.0 37.0 35.0 37.0 5 35.762337256043935 37.0 35.0 37.0 35.0 37.0 6 35.45502590326954 37.0 35.0 37.0 33.0 37.0 7 35.79534351099979 37.0 35.0 37.0 35.0 37.0 8 35.88598232714265 37.0 35.0 37.0 35.0 37.0 9 37.6456298931404 39.0 37.0 39.0 35.0 39.0 10 37.08684531035718 39.0 37.0 39.0 33.0 39.0 11 36.97007104953004 39.0 37.0 39.0 33.0 39.0 12 36.49166628954602 38.0 35.0 39.0 33.0 39.0 13 36.06206356483294 38.0 35.0 39.0 32.0 39.0 14 36.364432341604406 39.0 35.0 41.0 27.0 41.0 15 36.65647916228059 39.0 35.0 41.0 30.0 41.0 16 37.02211173133794 39.0 35.0 41.0 31.0 41.0 17 37.13064180687356 39.0 35.0 41.0 32.0 41.0 18 37.199072479965054 39.0 35.0 41.0 32.0 41.0 19 37.240616090524696 39.0 35.0 41.0 32.0 41.0 20 37.08987145760737 39.0 35.0 41.0 31.0 41.0 21 36.937975786886824 39.0 35.0 40.0 31.0 41.0 22 37.20525857031267 39.0 35.0 40.0 32.0 41.0 23 37.2351068497571 39.0 35.0 40.0 33.0 41.0 24 37.1583473851766 39.0 35.0 41.0 32.0 41.0 25 37.06355499501217 39.0 35.0 40.0 32.0 41.0 26 36.979277384369894 39.0 35.0 40.0 32.0 41.0 27 36.9385916413012 39.0 35.0 40.0 32.0 41.0 28 36.86643042517566 38.0 35.0 40.0 32.0 41.0 29 36.71713196795983 38.0 35.0 40.0 31.0 41.0 30 36.7025324799257 38.0 35.0 40.0 31.0 41.0 31 36.52064292839758 38.0 35.0 40.0 31.0 41.0 32 36.45240901390493 38.0 35.0 40.0 31.0 41.0 33 36.444851516123386 38.0 35.0 40.0 31.0 41.0 34 36.24990112880408 38.0 35.0 40.0 30.0 41.0 35 36.195570176905655 38.0 35.0 40.0 30.0 41.0 36 36.071781472029784 38.0 35.0 40.0 30.0 41.0 37 35.96374132540527 38.0 34.0 40.0 30.0 41.0 38 35.85255107361329 38.0 34.0 40.0 29.0 41.0 39 35.822376174894785 38.0 34.0 40.0 29.0 41.0 40 35.68880856765643 38.0 34.0 40.0 29.0 41.0 41 35.57450559483076 38.0 34.0 40.0 28.0 41.0 42 35.589339225597506 38.0 34.0 40.0 29.0 41.0 43 35.54056276894441 38.0 34.0 40.0 28.0 41.0 44 35.38778561970104 38.0 34.0 40.0 28.0 41.0 45 35.48698343489357 37.0 34.0 40.0 29.0 41.0 46 35.46320122305145 37.0 34.0 40.0 29.0 41.0 47 35.21090160692748 37.0 34.0 40.0 28.0 41.0 48 35.11690805667435 37.0 34.0 40.0 27.0 41.0 49 35.182412929401046 37.0 34.0 40.0 27.0 41.0 50 35.0430625869427 37.0 34.0 40.0 27.0 41.0 51 34.03469641131991 36.0 32.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 5.0 10 9.0 11 9.0 12 4.0 13 7.0 14 11.0 15 22.0 16 41.0 17 83.0 18 141.0 19 269.0 20 454.0 21 744.0 22 1142.0 23 1687.0 24 2412.0 25 3502.0 26 4798.0 27 5735.0 28 7041.0 29 8690.0 30 11255.0 31 14713.0 32 20119.0 33 29673.0 34 48044.0 35 44336.0 36 42295.0 37 61577.0 38 98305.0 39 101073.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.729480537623196 4.513248740253071 59.07834337130119 12.67892735082255 2 20.34424884453513 4.867807735367554 53.46914923549446 21.31879418460285 3 19.675466367068907 4.402867166302335 53.23264539968559 22.689021066943177 4 19.13083856547241 4.863085528995331 51.76777763130194 24.23829827423033 5 17.316134008346502 5.143860049543815 53.02132666452856 24.51867927758113 6 20.554977303895626 5.348095475142502 55.59079720681493 18.506130014146944 7 81.32308352205763 2.0104793629743605 11.364186393355856 5.302250721612161 8 82.90836755293299 2.5163457205988546 8.42343237505337 6.1518543514147925 9 77.70528316513753 5.0151799259007115 11.341952671686634 5.9375842372751295 10 42.72219456670805 25.17349189452952 18.435296918563584 13.669016620198846 11 33.47749180008539 21.907299153741267 26.02073442114604 18.5944746250273 12 32.40102550581717 19.07928781257563 30.141056239510306 18.378630442096895 13 28.53471903855878 24.12260421811084 29.877596475659978 17.4650802676704 14 18.273561350314914 26.479378715048295 35.81144229955709 19.435617635079698 15 15.509890070970828 24.167071661449285 40.68141437951192 19.64162388806797 16 23.297792171762385 23.330847616367954 31.56598201232889 21.805378199540765 17 23.772373912170895 23.64664516751043 27.206401737771944 25.374579182546725 18 25.237044921955704 21.27865543043895 32.12516995023975 21.3591296973656 19 25.263213815601777 25.275609607328864 28.538457451936793 20.922719125132566 20 22.046210724524187 31.226573429325295 28.907576583365636 17.819639262784882 21 19.632376233922365 26.74264172029978 34.36349577067392 19.261486275103938 22 16.93560287818478 23.910301689959606 34.7812142760169 24.372881155838712 23 17.377916208383095 24.842347172677314 33.17290948907694 24.606827129862644 24 20.482176622323838 28.63054047619516 29.48230845058506 21.404974450895942 25 22.887353734576585 26.658035522797434 25.46941682718889 24.98519391543709 26 16.25993384975907 28.029049439533132 26.603533390918017 29.107483319789786 27 15.965779744489284 26.253499843576915 32.42384950328292 25.356870908650887 28 15.865236100480681 25.447576622717357 29.710154908044867 28.977032368757094 29 16.87637853993314 29.05711311848606 29.235966684834043 24.830541656746753 30 22.73565285486889 25.81925361593115 30.134759964347342 21.31033356485262 31 22.596938042684812 27.759686917717524 28.346027542268665 21.297347497329003 32 24.264860685074087 27.031483343400815 25.746256175760525 22.957399795764573 33 25.549104059720168 24.807717659281003 25.903663054834652 23.73951522616417 34 17.944580973049977 24.512579761017005 31.28068204400703 26.262157221925992 35 16.649319116868703 29.354612119936174 28.140808323675763 25.855260439519355 36 24.45099431957925 26.313904733421612 26.10396330845649 23.131137638542647 37 20.403276424187926 29.23321206445025 27.852753734970104 22.510757776391724 38 21.24186157245537 28.1345120485128 27.561354250084115 23.06227212894772 39 18.832355771028084 25.569763712598647 29.057309877084904 26.54057063928836 40 23.381808093468205 22.60736624842347 30.210315266302924 23.8005103918054 41 18.900631004826487 22.026731623238767 28.07489419306347 30.997743178871275 42 20.600231781629436 22.657933208326035 30.28272243067703 26.4591125793675 43 21.532867540143673 22.79900912369623 29.671393464072864 25.996729872087236 44 21.74595710269028 23.20452859591096 27.8822675247965 27.167246776602255 45 18.59604869381804 24.508644589040152 25.912713950381416 30.98259276676039 46 19.367735918478974 24.060231742277715 29.972237361703304 26.599794977540004 47 15.922689611342738 25.507784753963207 33.304540991702694 25.26498464299136 48 17.645704661407965 27.06453878800638 31.07861096299561 24.211145587590043 49 23.07801281685513 21.784128270865757 31.73539116593243 23.402467746346684 50 19.67743395305733 22.91332586962382 28.622079856444927 28.787160320873923 51 17.60536914864522 23.97050982120546 26.155317302754423 32.268803727394896 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 51.0 1 281.0 2 511.0 3 1419.0 4 2327.0 5 1498.0 6 669.0 7 699.5 8 730.0 9 874.0 10 1018.0 11 1115.5 12 1213.0 13 1254.5 14 1296.0 15 1268.5 16 1241.0 17 1231.5 18 1222.0 19 1358.5 20 1495.0 21 1476.0 22 1457.0 23 1729.5 24 2002.0 25 2215.0 26 2985.5 27 3543.0 28 4112.5 29 4682.0 30 6129.5 31 7577.0 32 8170.5 33 8764.0 34 11392.0 35 14020.0 36 14072.5 37 14125.0 38 16185.0 39 18245.0 40 21292.0 41 24339.0 42 27911.0 43 31483.0 44 35683.0 45 39883.0 46 46414.0 47 52945.0 48 60852.5 49 68760.0 50 64118.5 51 59477.0 52 49001.5 53 38526.0 54 34694.0 55 30862.0 56 27538.0 57 24214.0 58 20636.5 59 17059.0 60 13788.5 61 10518.0 62 8277.0 63 6036.0 64 5845.5 65 5655.0 66 4667.0 67 3679.0 68 3001.5 69 2324.0 70 1906.0 71 1488.0 72 1351.5 73 1215.0 74 917.5 75 415.5 76 211.0 77 147.0 78 83.0 79 115.0 80 147.0 81 99.0 82 51.0 83 45.0 84 39.0 85 21.5 86 4.0 87 3.0 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 508237.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.501380092435582 #Duplication Level Percentage of deduplicated Percentage of total 1 68.64957097118193 20.252570864034702 2 12.63104281839177 7.452663902984089 3 5.529803627154916 4.8941051592367835 4 2.9374279807607255 3.4663271741831085 5 1.7566790923078914 2.5912228801304917 6 1.2243814249936147 2.167256507811272 7 0.8706433813726029 1.7979626923185554 8 0.6505562138446123 1.535384490890057 9 0.5015162049082682 1.3315878165163164 >10 4.39438987853814 29.765258682503497 >50 0.7149045354559456 13.583660368863217 >100 0.1283334269474489 6.892724463087799 >500 0.00671902758887167 1.4331348422087478 >1k 0.004031416553323002 2.836140155231364 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC 3600 0.7083309558335973 No Hit CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT 2755 0.5420699398115446 TruSeq Adapter, Index 13 (95% over 22bp) GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC 2709 0.533019044264782 No Hit CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC 2449 0.48186180856568883 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1687 0.3319317562475774 No Hit TCCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG 1108 0.21800852751767386 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 943 0.18554335870863398 No Hit TTCCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCT 935 0.18396928991789263 No Hit TTTTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA 768 0.15111060391116743 No Hit TGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG 761 0.14973329371926877 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC 760 0.1495365351204261 No Hit GCCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG 694 0.13655046759681014 No Hit TTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG 634 0.12474495166625019 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 634 0.12474495166625019 No Hit TTTTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATAC 561 0.1103815739507356 No Hit CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA 540 0.1062496433750396 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.5894887621326271 0.0 2 0.0 0.0 0.0 2.512410548622001 0.0 3 0.0 0.0 0.0 3.606388358187224 0.0 4 0.0 0.0 0.0 4.836916635349256 0.0 5 0.0 0.0 0.0 6.6587045020335 0.0 6 0.0 0.0 0.0 7.767635965110765 0.0 7 0.0 0.0 0.0 8.737655857405109 0.0 8 0.0 0.0 0.0 10.22121569267881 0.0 9 0.0 0.0 0.0 11.028909740927952 0.0 10 0.0 0.0 0.0 12.45167116915927 0.0 11 0.0 0.0 0.0 15.226360930038545 0.0 12 0.0 0.0 0.0 17.529813846689635 0.0 13 0.0 0.0 0.0 18.245818387878096 0.0 14 0.0 0.0 0.0 18.530921597601118 0.0 15 0.0 0.0 0.0 18.906337004192928 0.0 16 0.0 0.0 0.0 19.8433014518817 0.0 17 0.0 0.0 0.0 21.29951184191627 0.0 18 0.0 0.0 0.0 23.054008267796323 0.0 19 0.0 0.0 0.0 24.093287186883284 0.0 20 0.0 0.0 0.0 25.211466304106157 0.0 21 0.0 0.0 0.0 26.654297109419424 0.0 22 0.0 0.0 0.0 28.18389845682231 0.0 23 0.0 0.0 0.0 29.46676452127649 0.0 24 0.0 0.0 0.0 30.41002524412823 0.0 25 0.0 0.0 0.0 31.218506326772747 0.0 26 0.0 0.0 0.0 31.958515417020013 0.0 27 0.0 0.0 0.0 32.978118476222704 0.0 28 0.0 0.0 0.0 33.88911078886425 0.0 29 0.0 0.0 0.0 34.93606329330608 0.0 30 0.0 0.0 0.0 36.05699703091275 0.0 31 0.0 0.0 0.0 36.807237568299826 0.0 32 0.0 0.0 0.0 37.520684247703336 0.0 33 0.0 0.0 0.0 38.31224409084738 0.0 34 0.0 0.0 0.0 39.20690543978498 0.0 35 0.0 0.0 0.0 40.29104531940807 0.0 36 0.0 0.0 0.0 40.971830071403694 0.0 37 0.0 0.0 0.0 41.701017438714615 0.0 38 0.0 0.0 0.0 42.745215322772644 0.0 39 0.0 0.0 0.0 44.928645494129704 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGGCG 35 1.2101373E-7 45.000004 5 GCCGATG 35 1.2101373E-7 45.000004 9 TCAAGCG 65 0.0 45.000004 17 GTAGTAC 35 1.2101373E-7 45.000004 36 TGATCGG 20 7.02957E-4 45.0 13 ACAACGC 25 3.887723E-5 45.0 38 AGGTATG 25 3.887723E-5 45.0 17 TTCTACG 20 7.02957E-4 45.0 31 AACAACG 25 3.887723E-5 45.0 37 GGTACTA 25 3.887723E-5 45.0 8 TCGTTAG 20 7.02957E-4 45.0 1 GTACCGG 20 7.02957E-4 45.0 2 ACACGAG 20 7.02957E-4 45.0 29 TCACCGC 20 7.02957E-4 45.0 34 CGTATAT 20 7.02957E-4 45.0 16 GGTCGCG 20 7.02957E-4 45.0 44 CACGGGT 20 7.02957E-4 45.0 3 CATGCGT 20 7.02957E-4 45.0 26 ACCGCGT 20 7.02957E-4 45.0 36 GAGTAGA 20 7.02957E-4 45.0 15 >>END_MODULE