##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934412.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	689527
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10451222359676	33.0	31.0	34.0	30.0	34.0
2	32.30166621466599	34.0	31.0	34.0	30.0	34.0
3	32.359059181148815	34.0	31.0	34.0	30.0	34.0
4	35.896336184079814	37.0	35.0	37.0	35.0	37.0
5	35.87083319434917	37.0	35.0	37.0	35.0	37.0
6	35.56924239369887	37.0	35.0	37.0	33.0	37.0
7	35.90925663534568	37.0	35.0	37.0	35.0	37.0
8	35.999374933831454	37.0	35.0	37.0	35.0	37.0
9	37.7648561985245	39.0	38.0	39.0	35.0	39.0
10	37.182214764614	39.0	37.0	39.0	34.0	39.0
11	37.041180403377965	39.0	37.0	39.0	33.0	39.0
12	36.935181653510305	39.0	37.0	39.0	33.0	39.0
13	36.87399768246929	39.0	37.0	39.0	33.0	39.0
14	37.96067304108468	40.0	37.0	41.0	33.0	41.0
15	37.98102032262696	40.0	37.0	41.0	33.0	41.0
16	38.01920881995919	40.0	37.0	41.0	33.0	41.0
17	37.982881018437276	40.0	37.0	41.0	33.0	41.0
18	37.94228072287235	39.0	37.0	41.0	33.0	41.0
19	37.96769234562243	40.0	37.0	41.0	33.0	41.0
20	37.91198169179742	39.0	36.0	41.0	33.0	41.0
21	37.75693627660701	39.0	36.0	41.0	33.0	41.0
22	37.82798208046965	39.0	36.0	41.0	33.0	41.0
23	37.743637304993136	39.0	36.0	41.0	33.0	41.0
24	37.69619608804296	39.0	36.0	41.0	33.0	41.0
25	37.65552762981	39.0	36.0	41.0	33.0	41.0
26	37.61029372308844	39.0	36.0	41.0	33.0	41.0
27	37.55882510764626	39.0	36.0	41.0	33.0	41.0
28	37.46830508449995	39.0	36.0	41.0	33.0	41.0
29	37.424266199873244	39.0	36.0	41.0	33.0	41.0
30	37.38227799636562	39.0	36.0	41.0	32.0	41.0
31	37.32389159525298	39.0	36.0	41.0	32.0	41.0
32	37.20273317796112	39.0	35.0	41.0	32.0	41.0
33	37.12573691820625	39.0	35.0	41.0	32.0	41.0
34	37.00902212676226	39.0	35.0	41.0	31.0	41.0
35	36.94939284466018	39.0	35.0	41.0	31.0	41.0
36	36.77996655678458	39.0	35.0	40.0	31.0	41.0
37	36.706977391748254	39.0	35.0	40.0	31.0	41.0
38	36.63453207778666	39.0	35.0	40.0	31.0	41.0
39	36.559477728935924	39.0	35.0	40.0	30.0	41.0
40	36.498457638352086	39.0	35.0	40.0	30.0	41.0
41	36.45467690170218	39.0	35.0	40.0	30.0	41.0
42	36.3440184358263	39.0	35.0	40.0	30.0	41.0
43	36.26229864820377	39.0	35.0	40.0	30.0	41.0
44	36.14437143143053	38.0	35.0	40.0	30.0	41.0
45	36.18926017400334	38.0	35.0	40.0	30.0	41.0
46	36.08396480485899	38.0	35.0	40.0	30.0	41.0
47	35.84659048884235	38.0	35.0	40.0	29.0	41.0
48	35.75327434603721	38.0	35.0	40.0	28.0	41.0
49	35.76860079445765	38.0	35.0	40.0	28.0	41.0
50	35.65686477831905	38.0	34.0	40.0	28.0	41.0
51	34.66725450925055	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	10.0
11	5.0
12	7.0
13	9.0
14	8.0
15	10.0
16	21.0
17	49.0
18	92.0
19	182.0
20	316.0
21	607.0
22	959.0
23	1545.0
24	2327.0
25	3689.0
26	5196.0
27	6254.0
28	7466.0
29	9082.0
30	11536.0
31	14874.0
32	19791.0
33	27749.0
34	44618.0
35	55826.0
36	63768.0
37	93167.0
38	156959.0
39	163370.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.65832519973837	4.166624367138633	60.50161922593313	13.673431207189857
2	19.799949820674172	3.8519158785660315	54.75811679600654	21.59001750475326
3	18.704996323566736	4.004339206441517	55.13214130846218	22.15852316152957
4	18.24134515399687	4.083233869014556	53.413571912339904	24.261849064648665
5	16.78701486671298	4.7494876922876115	54.59467141968335	23.86882602131606
6	20.241846947255144	5.406459790552074	55.64959747769122	18.702095784501548
7	82.90117718377961	1.6815875230411572	10.491974933541398	4.925260359637839
8	85.51224245025938	1.7889074684530122	7.718189425504731	4.980660655782877
9	79.65300851163188	5.46229516755689	9.966542281883088	4.918154038928135
10	37.0229157088845	36.58623955262085	14.57201820958425	11.818826528910398
11	23.769482558333465	25.83640669618449	30.763552406214696	19.630558339267353
12	22.279330613594535	22.79664175587033	34.59429434960487	20.329733280930263
13	22.502382067707284	24.578732957520156	34.81618558809155	18.102699386681014
14	19.688859174477578	26.49685944132717	33.389990529739954	20.4242908544553
15	16.62516478687564	25.5415669002084	36.87542329741982	20.95784501549613
16	19.098019366899337	26.5466036862951	32.87920560036083	21.476171346444737
17	20.191232540567665	25.654832950703888	30.620845884207583	23.533088624520868
18	21.007879314370577	24.632828011085863	33.15867253929143	21.200620135252137
19	21.177415822730655	26.10934742221842	32.14986505241999	20.563371702630935
20	23.216349758602636	27.585286725537937	30.308747880793646	18.889615635065777
21	22.655240476442547	28.00847537514847	30.97065089546892	18.365633252940057
22	22.462064574701206	25.26877120112773	30.067423030570232	22.201741193600828
23	20.4994148162436	27.081318062962	28.84789138061309	23.571375740181313
24	21.122740661351912	26.235375844600718	29.501817912859106	23.140065581188264
25	19.22375773537512	28.440220615001298	28.299109389480037	24.03691226014355
26	18.616529882078584	29.145051607841317	29.15027257815865	23.088145931921446
27	19.813437327327286	27.68695061977268	31.469688641634047	21.029923411265983
28	16.263612592400296	26.155901074214643	34.13876468941753	23.44172164396753
29	18.041788066312122	24.267940196685554	33.72833841169381	23.96193332530851
30	21.7002379892303	25.685143583935073	30.352110939818168	22.26250748701646
31	22.594329156073655	27.601964825162757	27.91246753209084	21.891238486672748
32	20.47795082716123	28.08519463342262	27.92058904147336	23.516265497942793
33	21.81567944402467	27.203285730653043	27.453167171118753	23.527867654203533
34	20.18630162415685	25.347085755887733	30.041028125077045	24.425584494878372
35	18.73559701070444	25.139552185773727	30.617075183422838	25.507775620098993
36	21.81190874323993	26.23233027858227	30.026815483657636	21.928945494520157
37	20.99743737373591	26.73122299779414	31.525669045592124	20.74567058287783
38	18.257153091902133	27.04810689066563	31.118143306933593	23.576596710498645
39	20.06012817482129	25.860046089565746	30.15646957987142	23.923356155741544
40	22.634356595173212	24.462856421866004	27.587317102883567	25.315469880077213
41	20.67417229492101	25.616110754183662	26.372426315430726	27.3372906354646
42	20.35366272821804	26.815338630684515	27.303789409261714	25.527209231835734
43	20.608185031188047	25.803485577794632	30.1718424369169	23.416486954100417
44	19.651587247489946	24.03488188279792	30.069888488775636	26.2436423809365
45	21.48110226285555	23.034776013122038	28.874141259153014	26.6099804648694
46	22.98358149862152	23.29451928640938	30.235364242444458	23.486534972524645
47	19.071624461406152	24.990464477823203	32.05675774842754	23.8811533123431
48	18.640024248506585	24.380916193274523	32.260375590803555	24.718683967415345
49	21.723007220892	22.38955109807158	32.1327518719354	23.75468980910102
50	21.358699514304732	21.657600064972073	29.4868801366734	27.49682028404979
51	19.808651437869727	22.075277690358753	27.236206849042894	30.879864022728626
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	215.0
1	376.5
2	538.0
3	1849.0
4	3160.0
5	2117.0
6	1074.0
7	1252.0
8	1430.0
9	1705.5
10	1981.0
11	2245.0
12	2509.0
13	2769.0
14	3029.0
15	2941.5
16	2854.0
17	2779.5
18	2705.0
19	2736.0
20	2767.0
21	2797.5
22	2828.0
23	3166.5
24	3505.0
25	3934.5
26	5150.5
27	5937.0
28	7432.0
29	8927.0
30	10429.0
31	11931.0
32	13376.5
33	14822.0
34	16499.0
35	18176.0
36	20323.5
37	22471.0
38	24817.5
39	27164.0
40	30784.5
41	34405.0
42	40489.0
43	46573.0
44	51908.5
45	57244.0
46	66159.5
47	75075.0
48	83787.0
49	92499.0
50	87346.5
51	82194.0
52	66710.0
53	51226.0
54	41863.5
55	32501.0
56	28100.5
57	23700.0
58	20125.0
59	16550.0
60	15221.5
61	13893.0
62	11450.0
63	9007.0
64	7083.5
65	5160.0
66	3942.0
67	2724.0
68	2288.5
69	1853.0
70	1436.0
71	1019.0
72	884.0
73	749.0
74	561.5
75	256.0
76	138.0
77	141.5
78	145.0
79	87.0
80	29.0
81	30.5
82	32.0
83	32.0
84	32.0
85	21.0
86	10.0
87	7.5
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	689527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.87588290185605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.02057128651859	24.420292449096866
2	12.85879387141671	8.96923578637267
3	5.315133235998477	5.561098930393383
4	2.7119564038682835	3.783274959049955
5	1.571119019861415	2.739708148078278
6	1.067318399930792	2.2334202920989603
7	0.7133893945847002	1.741605949227339
8	0.5804640781335513	1.6195357774175645
9	0.3980857147633487	1.2495231695689395
>10	4.423114742572333	33.5132711400688
>50	0.25849112037042876	5.808470750335983
>100	0.07528867567508336	4.742558446750239
>500	0.0029278929429199085	0.7479036989476939
>1k	0.0033461633633370385	2.8701005025933055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGCT	4139	0.6002665595400906	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGC	3869	0.5611092821600895	No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGC	3097	0.4491484742439382	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGC	2943	0.42681432344201176	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1988	0.28831358307941535	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCC	1386	0.20100735721733884	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTG	1238	0.17954336813496788	No Hit
TTTTTGGGAAACTTCTCATTTTACTGCCACATTAGCATTTTATCAGTAAAA	1015	0.14720235755815217	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTG	958	0.1389358212223742	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTG	937	0.13589025520392967	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCT	779	0.11297599658896605	No Hit
CTACACAAAAATAAATAAAGGTAAAAACTCTCATACGCGCATGTCATCGGG	749	0.10862518799118816	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.801078130370529E-4	0.0	0.0	0.6455149689569807	0.0
2	5.801078130370529E-4	0.0	0.0	2.2258736786231723	0.0
3	5.801078130370529E-4	0.0	0.0	3.1014013954493445	0.0
4	5.801078130370529E-4	0.0	0.0	4.036535190065074	0.0
5	5.801078130370529E-4	0.0	0.0	5.544235396148374	0.0
6	5.801078130370529E-4	0.0	0.0	6.443402506355806	0.0
7	5.801078130370529E-4	0.0	0.0	7.324151193499312	0.0
8	5.801078130370529E-4	0.0	0.0	8.827210537078315	0.0
9	5.801078130370529E-4	0.0	0.0	9.584686313951448	0.0
10	5.801078130370529E-4	0.0	0.0	11.064831398915489	0.0
11	5.801078130370529E-4	0.0	0.0	14.257019667105132	0.0
12	5.801078130370529E-4	0.0	0.0	16.972359312978316	0.0
13	5.801078130370529E-4	0.0	0.0	17.814675857508117	0.0
14	5.801078130370529E-4	0.0	0.0	18.07224372649657	0.0
15	5.801078130370529E-4	0.0	0.0	18.43452105573821	0.0
16	5.801078130370529E-4	0.0	0.0	19.340794486655344	0.0
17	5.801078130370529E-4	0.0	0.0	20.833121835693163	0.0
18	5.801078130370529E-4	0.0	0.0	22.401298281285577	0.0
19	5.801078130370529E-4	0.0	0.0	23.549766724145684	0.0
20	5.801078130370529E-4	0.0	0.0	24.68764819941786	0.0
21	5.801078130370529E-4	0.0	0.0	26.242047084450647	0.0
22	5.801078130370529E-4	0.0	0.0	27.8139942308278	0.0
23	5.801078130370529E-4	0.0	0.0	29.258027604430282	0.0
24	7.251347662963162E-4	0.0	0.0	30.36545341951802	0.0
25	7.251347662963162E-4	0.0	0.0	31.27636771293945	0.0
26	7.251347662963162E-4	0.0	0.0	32.137827815299474	0.0
27	7.251347662963162E-4	0.0	0.0	33.01060002001372	0.0
28	7.251347662963162E-4	0.0	0.0	33.856542238375	0.0
29	7.251347662963162E-4	0.0	0.0	34.76513610054429	0.0
30	8.701617195555794E-4	0.0	0.0	35.80135368158172	0.0
31	8.701617195555794E-4	0.0	0.0	36.72938115548775	0.0
32	8.701617195555794E-4	0.0	0.0	37.5497986300754	0.0
33	8.701617195555794E-4	0.0	0.0	38.39487068671713	0.0
34	8.701617195555794E-4	0.0	0.0	39.24530874062945	0.0
35	8.701617195555794E-4	0.0	0.0	40.14940676724769	0.0
36	8.701617195555794E-4	0.0	0.0	40.9831667215352	0.0
37	8.701617195555794E-4	0.0	0.0	41.79372236330122	0.0
38	8.701617195555794E-4	0.0	0.0	42.64416041721354	0.0
39	8.701617195555794E-4	0.0	0.0	43.515482352395196	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTC	60	0.0	45.000004	5
TACTTCG	35	1.210974E-7	45.000004	1
TTATGCG	30	2.1642336E-6	45.000004	1
ACGATGG	25	3.8891754E-5	45.000004	10
GCCGATA	25	3.8891754E-5	45.000004	9
GAGCGTA	35	1.210974E-7	45.000004	32
GTCGTGA	30	2.1642336E-6	45.000004	22
CGTACAA	70	0.0	45.000004	26
ATCGGGA	30	2.1642336E-6	45.000004	4
TATCGCG	25	3.8891754E-5	45.000004	1
AGTGTCG	30	2.1642336E-6	45.000004	19
GCTTTCG	70	0.0	45.000004	1
AACCGGT	20	7.031322E-4	45.0	36
GTCGATT	20	7.031322E-4	45.0	26
CGCGGAC	20	7.031322E-4	45.0	4
CGACGCC	20	7.031322E-4	45.0	45
ACCGAAT	20	7.031322E-4	45.0	25
CGACCAA	20	7.031322E-4	45.0	29
TACGGGT	20	7.031322E-4	45.0	4
CTAACGT	20	7.031322E-4	45.0	19
>>END_MODULE