SRR2934411.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934411.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	191445
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2546	1.3298858680038654	No Hit
TCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC	1200	0.6268118780850898	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGCT	1004	0.5244326046645251	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC	654	0.34161247355637386	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCC	639	0.33377732508031027	No Hit
CCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC	635	0.33168795215336	TruSeq Adapter, Index 27 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCT	626	0.3269868630677218	No Hit
TTCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCT	369	0.1927446525111651	No Hit
TCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	366	0.19117762281595235	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCT	339	0.17707435555903783	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTC	307	0.16035937214343546	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTT	302	0.15774765598474758	No Hit
CGTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCT	288	0.15043485074042154	No Hit
CGCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	278	0.1452114184230458	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCG	254	0.13267518086134397	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTC	252	0.13163049439786884	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGC	247	0.12901877823918098	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGT	241	0.12588471884875552	No Hit
TTTCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTC	235	0.12275065945833008	No Hit
TTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	227	0.11857191360442948	No Hit
GCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	193	0.10081224372535194	TruSeq Adapter, Index 27 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTCT	30	2.1559954E-6	45.000004	1
ATGGGTA	20	7.0185435E-4	45.0	5
TCGTTCA	25	3.8785896E-5	45.0	16
CGTGCGG	25	3.8785896E-5	45.0	2
TAGCGGG	20	7.0185435E-4	45.0	3
CGTTCAT	25	3.8785896E-5	45.0	17
TTATCAC	25	3.8785896E-5	45.0	38
TCGGCAC	20	7.0185435E-4	45.0	35
TCGTGCG	25	3.8785896E-5	45.0	1
CCCCGAT	25	3.8785896E-5	45.0	40
ACGGCGG	25	3.8785896E-5	45.0	16
CGTAGGG	20	7.0185435E-4	45.0	3
TCTCGCG	20	7.0185435E-4	45.0	1
GCGGGCT	35	1.204844E-7	45.0	5
CGGGACG	20	7.0185435E-4	45.0	6
AATTATC	25	3.8785896E-5	45.0	45
ATGCGGG	25	3.8785896E-5	45.0	3
CTCTCGG	25	3.8785896E-5	45.0	2
CGATCCC	25	3.8785896E-5	45.0	43
CCTGCGG	20	7.0185435E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content