##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934411.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	191445
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04917861526809	33.0	31.0	34.0	30.0	34.0
2	32.27408394055734	34.0	31.0	34.0	30.0	34.0
3	32.24724594531067	33.0	31.0	34.0	30.0	34.0
4	35.84396040638303	37.0	35.0	37.0	35.0	37.0
5	35.85086056047429	37.0	35.0	37.0	35.0	37.0
6	35.57106740839406	37.0	35.0	37.0	33.0	37.0
7	35.87326386168351	37.0	35.0	37.0	35.0	37.0
8	35.967431899501165	37.0	35.0	37.0	35.0	37.0
9	37.741758729661264	39.0	38.0	39.0	35.0	39.0
10	37.2282274282431	39.0	37.0	39.0	34.0	39.0
11	37.13122829010943	39.0	37.0	39.0	33.0	39.0
12	37.07479432735251	39.0	37.0	39.0	33.0	39.0
13	37.062608059756066	39.0	37.0	39.0	33.0	39.0
14	38.14499203426572	40.0	38.0	41.0	33.0	41.0
15	38.17352764501554	40.0	38.0	41.0	33.0	41.0
16	38.211804957037266	40.0	38.0	41.0	33.0	41.0
17	38.17125545195748	40.0	37.0	41.0	33.0	41.0
18	38.046906422210036	39.0	37.0	41.0	33.0	41.0
19	37.935182428373686	39.0	37.0	41.0	34.0	41.0
20	37.76396876387474	39.0	36.0	41.0	33.0	41.0
21	37.611078899945156	39.0	35.0	41.0	33.0	41.0
22	37.70151218365588	39.0	35.0	41.0	33.0	41.0
23	37.63210843845491	39.0	35.0	41.0	33.0	41.0
24	37.53521376896759	39.0	35.0	41.0	33.0	41.0
25	37.44805035388754	39.0	35.0	41.0	33.0	41.0
26	37.36106453550628	39.0	35.0	41.0	33.0	41.0
27	37.25884196505524	39.0	35.0	41.0	33.0	41.0
28	37.12872104259709	39.0	35.0	41.0	32.0	41.0
29	36.979367442346366	39.0	35.0	41.0	32.0	41.0
30	36.859881428086396	39.0	35.0	40.0	31.0	41.0
31	36.713327587557785	39.0	35.0	40.0	31.0	41.0
32	36.39759199770169	39.0	35.0	40.0	30.0	41.0
33	36.1080414740526	39.0	35.0	40.0	30.0	41.0
34	35.777361644336494	39.0	35.0	40.0	27.0	41.0
35	35.56105408864165	39.0	35.0	40.0	25.0	41.0
36	35.30562824832197	38.0	35.0	40.0	25.0	41.0
37	35.274115281151246	38.0	35.0	40.0	24.0	41.0
38	35.2101909164512	38.0	35.0	40.0	24.0	41.0
39	35.06196035414871	38.0	35.0	40.0	23.0	41.0
40	34.896387996552534	38.0	34.0	40.0	23.0	41.0
41	34.80163493431534	38.0	34.0	40.0	23.0	41.0
42	34.76391130611925	38.0	34.0	40.0	22.0	41.0
43	34.67538457520437	38.0	34.0	40.0	22.0	41.0
44	34.50400376087127	38.0	34.0	40.0	22.0	41.0
45	34.46291624226279	38.0	33.0	40.0	22.0	41.0
46	34.28665674214526	38.0	33.0	40.0	21.0	41.0
47	34.05401551359398	37.0	33.0	40.0	20.0	41.0
48	33.93255504191804	37.0	33.0	40.0	20.0	41.0
49	33.91220454960955	37.0	33.0	40.0	20.0	41.0
50	33.76132048368983	37.0	33.0	40.0	20.0	41.0
51	32.918206273342214	36.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	12.0
11	5.0
12	5.0
13	11.0
14	4.0
15	8.0
16	11.0
17	32.0
18	64.0
19	110.0
20	218.0
21	375.0
22	607.0
23	893.0
24	1438.0
25	2552.0
26	3598.0
27	3667.0
28	3706.0
29	3445.0
30	3872.0
31	4580.0
32	5661.0
33	7500.0
34	11119.0
35	13772.0
36	17564.0
37	25240.0
38	40203.0
39	41153.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.914100655540757	4.521925357152185	55.68074381676199	18.883230170545065
2	26.609208911175536	3.9102614327874847	49.589699391470134	19.890830264566848
3	17.517824962783042	3.890412389981457	57.333437801979684	21.258324845255817
4	16.271514011857192	3.9290657891300373	56.133093055446736	23.666327143566036
5	15.73976860194834	4.887043276136749	56.26002246075896	23.113165661155946
6	19.157460367207293	5.344615947138865	58.561989083026454	16.935934602627388
7	75.05811068453082	1.433309827887905	18.416255321371676	5.092324166209616
8	77.00383922275327	1.6986601896105928	15.588289064744442	5.7092115228916915
9	71.65243281360182	4.5443861161169	18.01666274909243	5.786518321188853
10	32.1152289169213	27.99811956436574	24.98002037138604	14.906631147326909
11	20.82269058998668	25.161795816030715	34.63971375590901	19.375799838073597
12	19.295358980386013	20.963200919324088	40.32124108751861	19.420199012771292
13	21.028493823291285	22.363603123612528	39.07022904750711	17.537674005589075
14	17.74295489566194	24.855180339000757	38.499307895217946	18.902556870119355
15	15.394499725769803	26.630624983676775	39.03575439421243	18.939120896340985
16	18.108072814646505	26.062315547546294	35.260779858444984	20.568831779362217
17	18.11747499281778	26.50474026482802	33.584580427799104	21.793204314555094
18	18.6988430097417	25.24275901695004	35.665073519809866	20.393324453498394
19	19.08903340384967	28.270260388101022	34.09229804904803	18.54840815900128
20	19.612421322050718	28.680299825015016	34.131996134660085	17.575282718274178
21	19.916425082921986	27.842983624539684	34.98132622946538	17.259265063072945
22	18.179111494162814	26.12708610828175	34.04685418788686	21.646948209668572
23	18.161351824283738	27.108046697484916	33.09357778996579	21.637023688265558
24	18.90986967536368	28.080649794980282	32.00292512209773	21.006555407558306
25	18.15038261641725	29.276815795659328	30.490219122985714	22.082582464937712
26	17.601922223092796	30.906004335448824	31.014129384418503	20.47794405703988
27	17.655723575961765	29.656036981900808	32.35602914675233	20.332210295385096
28	16.806915824388206	29.182271670714826	31.653477500065293	22.357335004831675
29	18.028154300190653	30.201363315834833	30.775940870746165	20.99454151322834
30	18.99866802475907	29.565149259578472	31.082034004544383	20.354148711118075
31	19.10627073049701	30.32254694559795	29.99973882838413	20.571443495520906
32	19.60824257619682	30.354409882733947	29.186450416568725	20.85089712450051
33	19.226409673796653	28.974379064483273	30.522604403353444	21.276606858366634
34	18.207840371908382	28.69283606257672	31.067408394055736	22.031915171459165
35	18.810624461333543	30.15069602235629	28.2963775496879	22.742301966622268
36	19.24207997074878	31.446107237065473	28.399279166340204	20.91253362584554
37	18.38909347332132	30.804669748491737	29.10600955888114	21.700227219305805
38	20.585024419546084	29.831544307764634	28.032071874428688	21.551359398260598
39	20.09349943848103	27.47107524354253	31.071587139909635	21.363838178066807
40	20.468019535636866	28.64478048525686	31.059050902347934	19.82814907675834
41	18.34835070124579	30.180469586565334	28.58941210269268	22.8817676094962
42	19.283867429287785	30.540886416464257	27.191621614562926	22.983624539685028
43	19.137611324401263	29.826320875447255	27.658074120504583	23.377993679646895
44	18.944866671890097	29.160333254981847	27.272584815482254	24.6222152576458
45	20.46540781947818	28.38987698816893	27.219305805845025	23.925409386507873
46	19.58421478753689	29.673274308548148	29.633053879704352	21.10945702421061
47	17.17830186215362	30.71691608555982	30.644832719580034	21.459949332706522
48	17.765415654626654	29.839379456240696	29.529107576588576	22.866097312544074
49	19.078586539214918	28.104155240408474	30.18099192979707	22.63626629057954
50	18.396406278565646	27.542636266290582	29.051163519548695	25.00979393559508
51	17.493797174123117	28.095797748700672	27.86492204027266	26.54548303690355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22.0
1	65.0
2	108.0
3	1923.0
4	3738.0
5	2552.0
6	1366.0
7	1393.5
8	1421.0
9	1617.5
10	1814.0
11	1925.5
12	2037.0
13	2131.0
14	2225.0
15	2283.0
16	2341.0
17	2277.5
18	2214.0
19	2227.0
20	2240.0
21	2247.5
22	2255.0
23	2321.0
24	2387.0
25	2476.5
26	2698.5
27	2831.0
28	3008.5
29	3186.0
30	3359.5
31	3533.0
32	3922.0
33	4311.0
34	4795.0
35	5279.0
36	5710.0
37	6141.0
38	6585.0
39	7029.0
40	7899.0
41	8769.0
42	9863.0
43	10957.0
44	12470.0
45	13983.0
46	16507.0
47	19031.0
48	20735.5
49	22440.0
50	20243.0
51	18046.0
52	14566.0
53	11086.0
54	9339.0
55	7592.0
56	6870.0
57	6148.0
58	5436.0
59	4724.0
60	3978.5
61	3233.0
62	2820.0
63	2407.0
64	2040.0
65	1673.0
66	1300.0
67	927.0
68	753.0
69	579.0
70	460.0
71	341.0
72	288.0
73	235.0
74	173.5
75	94.0
76	76.0
77	50.5
78	25.0
79	17.0
80	9.0
81	7.0
82	5.0
83	3.0
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	191445.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.33665351294491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6523948130479	45.4829659195928
2	12.91959366117616	15.332109052024986
3	4.9540982426699545	8.818788326831891
4	2.291740456875201	5.439368377248076
5	1.1110593221735308	3.2963271016069116
6	0.5781654249734117	2.0583840896887127
7	0.3724982167875088	1.5471958336597176
8	0.19965542086568042	0.9477507623910457
9	0.16488890007930632	0.8805559978922757
>10	0.6873674594518877	7.11045257303523
>50	0.035758999251898106	1.4941144579580887
>100	0.02582594390414863	3.2870518075077952
>500	0.003973222139099789	1.5053129489644808
>1k	0.002979916604324842	2.7996227515979966
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2546	1.3298858680038654	No Hit
TCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC	1200	0.6268118780850898	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGCT	1004	0.5244326046645251	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC	654	0.34161247355637386	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCC	639	0.33377732508031027	No Hit
CCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC	635	0.33168795215336	TruSeq Adapter, Index 27 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCT	626	0.3269868630677218	No Hit
TTCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCT	369	0.1927446525111651	No Hit
TCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	366	0.19117762281595235	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCT	339	0.17707435555903783	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTC	307	0.16035937214343546	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTT	302	0.15774765598474758	No Hit
CGTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCT	288	0.15043485074042154	No Hit
CGCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	278	0.1452114184230458	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCG	254	0.13267518086134397	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTC	252	0.13163049439786884	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGC	247	0.12901877823918098	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGT	241	0.12588471884875552	No Hit
TTTCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTC	235	0.12275065945833008	No Hit
TTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	227	0.11857191360442948	No Hit
GCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	193	0.10081224372535194	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5808456736921831	0.0
2	0.0	0.0	0.0	2.0397503199352296	0.0
3	0.0	0.0	0.0	2.979968137062864	0.0
4	0.0	0.0	0.0	4.691686907466896	0.0
5	0.0	0.0	0.0	6.774791715636344	0.0
6	0.0	0.0	0.0	8.293243490297474	0.0
7	0.0	0.0	0.0	9.537987411528116	0.0
8	0.0	0.0	0.0	11.109718195826478	0.0
9	0.0	0.0	0.0	12.169030269790278	0.0
10	0.0	0.0	0.0	13.80344224189715	0.0
11	0.0	0.0	0.0	16.369192196192117	0.0
12	0.0	0.0	0.0	18.296116378072032	0.0
13	0.0	0.0	0.0	19.018517067565096	0.0
14	0.0	0.0	0.0	19.31938676904594	0.0
15	0.0	0.0	0.0	19.671446107237067	0.0
16	0.0	0.0	0.0	20.45861735746559	0.0
17	0.0	0.0	0.0	21.689258011439318	0.0
18	0.0	0.0	0.0	23.133014703961972	0.0
19	0.0	0.0	0.0	23.89824753845752	0.0
20	0.0	0.0	0.0	24.641541957220088	0.0
21	0.0	0.0	0.0	25.5133328109901	0.0
22	0.0	0.0	0.0	26.376243829820574	0.0
23	0.0	0.0	0.0	27.291389171824807	0.0
24	0.0	0.0	0.0	27.931259630703334	0.0
25	0.0	0.0	0.0	28.488599858967326	0.0
26	0.0	0.0	0.0	28.97960249680065	0.0
27	0.0	0.0	0.0	29.488887147744784	0.0
28	0.0	0.0	0.0	29.971009950638564	0.0
29	0.0	0.0	0.0	30.51267988195043	0.0
30	0.0	0.0	0.0	31.11546397137559	0.0
31	0.0	0.0	0.0	31.62788268171015	0.0
32	0.0	0.0	0.0	32.13403327326386	0.0
33	0.0	0.0	0.0	32.63234871634151	0.0
34	0.0	0.0	0.0	33.14424508344433	0.0
35	0.0	0.0	0.0	33.649350988534565	0.0
36	0.0	0.0	0.0	34.13513019405051	0.0
37	0.0	0.0	0.0	34.61516362401734	0.0
38	0.0	0.0	0.0	35.14220794484056	0.0
39	0.0	0.0	0.0	35.711562067434514	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTCT	30	2.1559954E-6	45.000004	1
ATGGGTA	20	7.0185435E-4	45.0	5
TCGTTCA	25	3.8785896E-5	45.0	16
CGTGCGG	25	3.8785896E-5	45.0	2
TAGCGGG	20	7.0185435E-4	45.0	3
CGTTCAT	25	3.8785896E-5	45.0	17
TTATCAC	25	3.8785896E-5	45.0	38
TCGGCAC	20	7.0185435E-4	45.0	35
TCGTGCG	25	3.8785896E-5	45.0	1
CCCCGAT	25	3.8785896E-5	45.0	40
ACGGCGG	25	3.8785896E-5	45.0	16
CGTAGGG	20	7.0185435E-4	45.0	3
TCTCGCG	20	7.0185435E-4	45.0	1
GCGGGCT	35	1.204844E-7	45.0	5
CGGGACG	20	7.0185435E-4	45.0	6
AATTATC	25	3.8785896E-5	45.0	45
ATGCGGG	25	3.8785896E-5	45.0	3
CTCTCGG	25	3.8785896E-5	45.0	2
CGATCCC	25	3.8785896E-5	45.0	43
CCTGCGG	20	7.0185435E-4	45.0	2
>>END_MODULE