##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934409.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	913088
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10197155148244	33.0	31.0	34.0	30.0	34.0
2	32.28474911509077	34.0	31.0	34.0	30.0	34.0
3	32.359039873484264	34.0	31.0	34.0	30.0	34.0
4	35.88490265998458	37.0	35.0	37.0	35.0	37.0
5	35.84619226186304	37.0	35.0	37.0	35.0	37.0
6	35.496997003574684	37.0	35.0	37.0	33.0	37.0
7	35.86253898857503	37.0	35.0	37.0	35.0	37.0
8	35.9371604927455	37.0	35.0	37.0	35.0	37.0
9	37.712434069881546	39.0	38.0	39.0	35.0	39.0
10	37.150143250157704	39.0	37.0	39.0	34.0	39.0
11	37.030435182589194	39.0	37.0	39.0	33.0	39.0
12	36.89497507359641	39.0	37.0	39.0	33.0	39.0
13	36.81278474801991	39.0	37.0	39.0	33.0	39.0
14	37.92696541844817	40.0	37.0	41.0	33.0	41.0
15	37.97533425036798	40.0	37.0	41.0	33.0	41.0
16	38.017174686339104	40.0	37.0	41.0	33.0	41.0
17	37.94944298906567	40.0	37.0	41.0	33.0	41.0
18	37.93468208978762	40.0	37.0	41.0	33.0	41.0
19	37.99749093187075	40.0	37.0	41.0	33.0	41.0
20	37.95073311663279	40.0	37.0	41.0	33.0	41.0
21	37.77181498387888	40.0	36.0	41.0	33.0	41.0
22	37.86175593152029	40.0	36.0	41.0	33.0	41.0
23	37.81825410037148	39.0	36.0	41.0	33.0	41.0
24	37.77612125008761	40.0	36.0	41.0	33.0	41.0
25	37.69308215637485	39.0	36.0	41.0	33.0	41.0
26	37.65368398226677	39.0	36.0	41.0	33.0	41.0
27	37.56396426193313	39.0	36.0	41.0	33.0	41.0
28	37.47099512861849	39.0	36.0	41.0	33.0	41.0
29	37.4139338157987	39.0	36.0	41.0	32.0	41.0
30	37.379005090418445	39.0	36.0	41.0	32.0	41.0
31	37.34021802936847	39.0	36.0	41.0	32.0	41.0
32	37.25236450374991	39.0	35.0	41.0	32.0	41.0
33	37.19326505221841	39.0	35.0	41.0	32.0	41.0
34	37.07767159353753	39.0	35.0	41.0	31.0	41.0
35	37.03196406041915	39.0	35.0	41.0	31.0	41.0
36	36.863542177752855	39.0	35.0	41.0	31.0	41.0
37	36.84560524286816	39.0	35.0	41.0	31.0	41.0
38	36.76414650066587	39.0	35.0	41.0	31.0	41.0
39	36.678521675895425	39.0	35.0	41.0	31.0	41.0
40	36.5722778089297	39.0	35.0	40.0	30.0	41.0
41	36.53078454650592	39.0	35.0	40.0	30.0	41.0
42	36.50881076084671	39.0	35.0	40.0	30.0	41.0
43	36.443738172005325	39.0	35.0	40.0	30.0	41.0
44	36.261778711361885	39.0	35.0	40.0	30.0	41.0
45	36.27011744760637	39.0	35.0	40.0	30.0	41.0
46	36.17040088140464	38.0	35.0	40.0	30.0	41.0
47	35.967911088525966	38.0	35.0	40.0	29.0	41.0
48	35.90214743814397	38.0	35.0	40.0	29.0	41.0
49	35.907652931590384	38.0	35.0	40.0	29.0	41.0
50	35.790471455106186	38.0	34.0	40.0	29.0	41.0
51	34.825034388799324	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	15.0
11	15.0
12	8.0
13	19.0
14	17.0
15	26.0
16	38.0
17	81.0
18	162.0
19	282.0
20	518.0
21	865.0
22	1395.0
23	2146.0
24	3155.0
25	4599.0
26	6470.0
27	8097.0
28	10017.0
29	12460.0
30	15838.0
31	20068.0
32	26527.0
33	35823.0
34	59240.0
35	72340.0
36	79150.0
37	117631.0
38	196544.0
39	239470.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.83538059858415	4.246688161491554	57.04247564309245	14.875455596831848
2	19.76315535851966	4.352921076610359	54.174186934884695	21.70973662998528
3	20.139132263264877	4.420274935165066	53.20954825821826	22.231044543351793
4	19.45288953529123	5.077057194925352	51.34444872783346	24.125604541949954
5	17.400732459521972	5.690251103946169	52.00188809840891	24.90712833812294
6	21.132136223452726	6.358970876848671	53.9083856101493	18.60050728954931
7	83.93517382771431	2.0582901100441577	9.274681082217704	4.731854980023831
8	85.34270519380388	2.7299668816149154	7.148489521272867	4.778838403308334
9	79.99787534169762	5.26280051867947	9.507407829256326	5.2319163103665804
10	38.40681292493166	30.18799940421953	16.66903956683255	14.73614810401626
11	25.899146632088033	26.43666327889535	27.945718265928367	19.718471823088247
12	23.513724854559474	23.947417992570266	31.8574989486227	20.681358204247562
13	22.112107485806405	24.03141865844256	33.497538024812506	20.358935830938528
14	19.290802200883157	26.729844220929415	32.40082007429733	21.578533503890096
15	17.941096586528353	25.873628828765682	35.59153115581412	20.593743428891848
16	20.509742763019556	26.045572825401276	31.78762616527651	21.657058246302658
17	20.543912525408285	25.67101966075559	29.938078257517347	23.84698955631878
18	20.27942542230322	25.02716058036027	32.48241133384734	22.21100266348917
19	22.328625499404218	25.893670708628303	31.049581201373798	20.728122590593678
20	24.342232074016962	26.745943435901033	29.90982249246513	19.002001997616876
21	23.69683973505292	27.121153711361885	30.366952582883577	18.81505397070162
22	22.24133927945609	24.834846148454474	29.369239328520365	23.554575243569076
23	20.76820635031892	25.942844501296698	29.055906988154483	24.2330421602299
24	21.082743393845938	25.304680381299505	30.267400294385645	23.345175930468915
25	20.07813047592346	27.111297049134365	28.865454370224995	23.94511810471718
26	20.734584180276162	27.552875516927173	28.572711502067712	23.139828800728953
27	19.835656585126515	26.810449814256675	30.356219772902506	22.997673827714305
28	18.586598443961588	25.88206175089367	32.24464673722577	23.286693067918975
29	20.819241956963623	24.131628057755663	31.375617684166258	23.67351230111446
30	21.35445871591785	24.723575383752717	31.12810594378636	22.793859956543074
31	21.80425106890026	26.253548398401904	28.440303672811385	23.50189685988645
32	21.946296523445714	26.655590698815445	28.842345973224926	22.55576680451391
33	21.37723855751034	25.22560804654097	29.908946344711573	23.48820705123712
34	19.883735193102964	24.283858729936217	31.198416801009323	24.633989275951496
35	21.235959732249245	24.040508691385714	29.41896071353473	25.304570862830307
36	21.30988469895563	25.30741834302937	30.269700182238736	23.112996775776267
37	20.311514333777247	25.01489451181047	31.615572650171725	23.058018504240554
38	20.23211344361113	26.040973049695097	30.725954124903627	23.000959381790146
39	21.09500946239574	23.99604419289269	32.18276880213079	22.726177542580782
40	21.168058281348568	23.688735368332516	31.218568199341135	23.92463815097778
41	18.65406182098549	24.397100827083477	30.6752470736665	26.273590278264525
42	20.793943190579657	23.06382298310787	30.967880423354593	25.174353402957877
43	21.66067235578608	23.087807527861496	31.1354436812224	24.116076435130022
44	21.2635583864863	22.990007534870678	30.164014859465897	25.582419219177122
45	22.24429627812434	22.92254415784678	28.946826592836615	25.88633297119226
46	21.417212798766382	22.93809578047242	31.088350739468705	24.556340681292493
47	18.62098724328871	23.34035711782435	32.70966215742623	25.328993481460714
48	18.870141760706527	22.329282610219387	34.38387099600477	24.416704633069322
49	20.984395808509145	21.00947553795472	34.10821300904184	23.89791564449429
50	20.980015069741363	21.55903921637345	31.47046067848882	25.99048503539637
51	19.487825926964323	21.386547627391884	30.43091136889325	28.694715076750544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	516.0
2	850.0
3	2336.5
4	3823.0
5	2599.0
6	1375.0
7	1438.0
8	1501.0
9	1659.0
10	1817.0
11	2018.5
12	2220.0
13	2334.0
14	2448.0
15	2320.5
16	2193.0
17	2204.5
18	2216.0
19	2829.5
20	3443.0
21	3373.5
22	3304.0
23	4370.0
24	5436.0
25	6002.0
26	7693.5
27	8819.0
28	10554.5
29	12290.0
30	14297.5
31	16305.0
32	18664.5
33	21024.0
34	25363.5
35	29703.0
36	32015.5
37	34328.0
38	35502.0
39	36676.0
40	41408.5
41	46141.0
42	52351.0
43	58561.0
44	63254.5
45	67948.0
46	76654.0
47	85360.0
48	95626.0
49	105892.0
50	100208.5
51	94525.0
52	80566.0
53	66607.0
54	58163.5
55	49720.0
56	42879.5
57	36039.0
58	33831.0
59	31623.0
60	28069.0
61	24515.0
62	21130.5
63	17746.0
64	15099.0
65	12452.0
66	9866.0
67	7280.0
68	6049.5
69	4819.0
70	3882.0
71	2945.0
72	2365.0
73	1785.0
74	1558.5
75	1025.5
76	719.0
77	521.5
78	324.0
79	249.5
80	175.0
81	104.5
82	34.0
83	25.5
84	17.0
85	9.5
86	2.0
87	2.5
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	913088.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.88989282032866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.49838090903951	19.38302394739487
2	12.579685694355375	7.016921714579862
3	5.381618084509783	4.502782547307608
4	2.778801290204001	3.100018806111223
5	1.6014005991615208	2.233144553651245
6	1.0075479638713172	1.6860242834226848
7	0.693165736956281	1.3532622677304362
8	0.5003942097781839	1.1164752702901282
9	0.3727074695262663	0.935529424058113
>10	4.310427462891472	30.592642568265386
>50	1.1849920853180063	21.278061610250774
>100	0.08532260390168402	4.181190475667307
>500	0.0027779452433106407	0.5559499102437163
>1k	0.0027779452433106407	2.0649726210266803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	4096	0.44858764982126587	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	3675	0.4024803742903203	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	3428	0.37542931239924304	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2655	0.29077153571178244	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2330	0.2551780332235228	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	1454	0.1592398542090138	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	1019	0.11159932010934323	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	983	0.10765665521833602	No Hit
TGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	927	0.10152362094343591	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1903693838928997E-4	0.0	0.0	0.43391217494918344	0.0
2	2.1903693838928997E-4	0.0	0.0	1.6849416485596131	0.0
3	2.1903693838928997E-4	0.0	0.0	2.4072159528982966	0.0
4	2.1903693838928997E-4	0.0	0.0	3.2270712132894093	0.0
5	2.1903693838928997E-4	0.0	0.0	4.481057685568095	0.0
6	2.1903693838928997E-4	0.0	0.0	5.225892794560875	0.0
7	2.1903693838928997E-4	0.0	0.0	5.932067883927946	0.0
8	2.1903693838928997E-4	0.0	0.0	7.074235999158899	0.0
9	2.1903693838928997E-4	0.0	0.0	7.664869103525619	0.0
10	2.1903693838928997E-4	0.0	0.0	8.762791757201935	0.0
11	2.1903693838928997E-4	0.0	0.0	10.904425422303218	0.0
12	2.1903693838928997E-4	0.0	0.0	12.704909055863181	0.0
13	2.1903693838928997E-4	0.0	0.0	13.297513492675405	0.0
14	2.1903693838928997E-4	0.0	0.0	13.491689738557511	0.0
15	2.1903693838928997E-4	0.0	0.0	13.785527791406743	0.0
16	2.1903693838928997E-4	0.0	0.0	14.498164470456297	1.0951846919464499E-4
17	2.1903693838928997E-4	0.0	0.0	15.594334828625499	1.0951846919464499E-4
18	3.2855540758393493E-4	0.0	0.0	16.885667098899557	1.0951846919464499E-4
19	3.2855540758393493E-4	0.0	0.0	17.721402537323893	1.0951846919464499E-4
20	4.3807387677857995E-4	0.0	0.0	18.59579799537394	1.0951846919464499E-4
21	5.47592345973225E-4	0.0	0.0	19.7439896264106	1.0951846919464499E-4
22	5.47592345973225E-4	0.0	0.0	20.896123922338262	1.0951846919464499E-4
23	5.47592345973225E-4	0.0	0.0	22.08527546085372	1.0951846919464499E-4
24	5.47592345973225E-4	0.0	0.0	22.95999947431135	1.0951846919464499E-4
25	5.47592345973225E-4	0.0	0.0	23.704286990958156	1.0951846919464499E-4
26	5.47592345973225E-4	0.0	0.0	24.35395055022079	1.0951846919464499E-4
27	5.47592345973225E-4	0.0	0.0	24.95772587089087	1.0951846919464499E-4
28	5.47592345973225E-4	0.0	0.0	25.617136573911825	1.0951846919464499E-4
29	5.47592345973225E-4	0.0	0.0	26.366242903203197	1.0951846919464499E-4
30	5.47592345973225E-4	0.0	0.0	27.21796803812995	0.0020808509146982546
31	5.47592345973225E-4	0.0	0.0	27.92688108922689	0.0020808509146982546
32	5.47592345973225E-4	0.0	0.0	28.65594553865564	0.0020808509146982546
33	5.47592345973225E-4	0.0	0.0	29.34328345132123	0.0020808509146982546
34	5.47592345973225E-4	0.0	0.0	30.018026740029438	0.0020808509146982546
35	5.47592345973225E-4	0.0	0.0	30.752129039041144	0.0020808509146982546
36	5.47592345973225E-4	0.0	0.0	31.422929662858344	0.0020808509146982546
37	5.47592345973225E-4	0.0	0.0	32.08781628933903	0.0020808509146982546
38	5.47592345973225E-4	0.0	0.0	32.76934972313731	0.0020808509146982546
39	5.47592345973225E-4	0.0	0.0	33.530831639447676	0.0020808509146982546
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	20	7.0325244E-4	45.0	24
CTATCGG	20	7.0325244E-4	45.0	2
GTTAGCG	20	7.0325244E-4	45.0	1
CCTCGAC	20	7.0325244E-4	45.0	17
CGTTAAC	25	3.890174E-5	45.0	6
TAACCGT	20	7.0325244E-4	45.0	12
TATTCGA	20	7.0325244E-4	45.0	41
TCGCTAG	20	7.0325244E-4	45.0	1
TGCTCGA	20	7.0325244E-4	45.0	14
CGGTCAC	20	7.0325244E-4	45.0	28
CGGTAAT	25	3.890174E-5	45.0	6
CGCTTAG	20	7.0325244E-4	45.0	32
ACGGTAC	20	7.0325244E-4	45.0	17
CCACGAA	30	2.1650103E-6	44.999996	15
TTTCGCG	230	0.0	44.02174	1
ACGTATA	80	0.0	42.1875	18
CGTTTGG	540	0.0	41.250004	2
CGGGACG	50	1.0804797E-9	40.5	6
GCGCGAC	185	0.0	40.135136	9
CGTTAGG	135	0.0	40.000004	2
>>END_MODULE