FastQCFastQC Report
Sat 14 Jan 2017
SRR2934407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048836
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440172.148390598369025No Hit
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC269821.3169428885474483No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG177620.8669312721955296No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC170680.8330583804657864No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC89300.43585723796340947No Hit
GAACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT78000.38070397044956256No Hit
CTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT76350.37265061722851417Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC71510.34902744778010536No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCC63390.30939518829227913No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCT60190.2937765638635791No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTT59780.29177542760865194No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTC55320.2700069698111513No Hit
GAATGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCT52130.254437153583791No Hit
CGTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC47560.23213180557155377No Hit
CCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC46890.22886165608179473No Hit
GAATGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTT41090.200552899304776No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACATCCGTT40290.196648243197601No Hit
CGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTG35820.17483097719876067No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGT35730.17439170338670348No Hit
CGTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT35530.17341553935990972No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTC33720.16458125491742628No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTA31530.1538922588240347No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG28780.14047000345562063No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC28200.13763912777791876No Hit
TTCCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT27410.13378327987208347No Hit
TCCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTG25890.12636443326845098No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT253.8918784E-545.028
GCCGAAC207.034581E-445.032
CGTTTTT228250.044.4578321
GTCCGCG700.041.7857131
CCGTAGT603.6379788E-1241.25000437
GTTTGCG3050.040.5737721
TTTGTCG4150.040.120481
TTACGCG451.929584E-840.01
GTTTTTT251150.039.965162
CCGATGA6450.039.76744518
CTCGTTG2000.039.3751
ACGAACG1150.039.13043621
CGATGAA6700.038.95522319
GCGCGAC2550.038.823539
CGTTTCT7950.038.7735861
CGGGTAC2150.038.7209326
GCGATAT356.2506842E-638.5714269
CGATAAC1050.038.57142616
GCCCGCG356.2506842E-638.5714261
CGTTTTC7450.038.3557051
Produced by FastQC (version 0.11.5)