##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934407.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2048836
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.899662052013923	33.0	31.0	34.0	30.0	34.0
2	32.11665794626803	33.0	31.0	34.0	30.0	34.0
3	32.092365616379254	33.0	31.0	34.0	30.0	34.0
4	35.782753719673025	37.0	35.0	37.0	35.0	37.0
5	35.77618706426478	37.0	35.0	37.0	35.0	37.0
6	35.35393413626078	37.0	35.0	37.0	33.0	37.0
7	35.79558197923114	37.0	35.0	37.0	35.0	37.0
8	35.79635802963244	37.0	35.0	37.0	35.0	37.0
9	37.633415754115994	39.0	37.0	39.0	35.0	39.0
10	37.11557196378822	39.0	37.0	39.0	33.0	39.0
11	37.01810442612293	39.0	37.0	39.0	33.0	39.0
12	36.999993166851816	39.0	37.0	39.0	33.0	39.0
13	36.945376789552704	39.0	37.0	39.0	33.0	39.0
14	38.08309840319089	40.0	37.0	41.0	33.0	41.0
15	38.04898147045444	40.0	37.0	41.0	33.0	41.0
16	38.11468023794974	40.0	37.0	41.0	33.0	41.0
17	37.99764207579328	40.0	37.0	41.0	33.0	41.0
18	37.82542721818633	39.0	37.0	41.0	33.0	41.0
19	37.69895687112097	39.0	37.0	41.0	33.0	41.0
20	37.422089908611525	39.0	35.0	41.0	33.0	41.0
21	37.333452262650596	39.0	35.0	41.0	33.0	41.0
22	37.44828282986047	39.0	35.0	41.0	33.0	41.0
23	37.37433498825675	39.0	35.0	41.0	33.0	41.0
24	37.282232448082716	39.0	35.0	41.0	33.0	41.0
25	37.20903283620553	39.0	35.0	40.0	32.0	41.0
26	37.150708499850644	39.0	35.0	40.0	32.0	41.0
27	37.06456836955227	39.0	35.0	40.0	32.0	41.0
28	36.911303296115456	39.0	35.0	40.0	32.0	41.0
29	36.79525008346202	39.0	35.0	40.0	31.0	41.0
30	36.71927523725667	39.0	35.0	40.0	31.0	41.0
31	36.556720498858866	39.0	35.0	40.0	31.0	41.0
32	36.19624557553655	38.0	35.0	40.0	30.0	41.0
33	35.86270545812354	39.0	35.0	40.0	28.0	41.0
34	35.46540279456237	38.0	35.0	40.0	25.0	41.0
35	35.25696004951104	38.0	35.0	40.0	24.0	41.0
36	34.96655613236003	38.0	34.0	40.0	23.0	41.0
37	34.93427536415799	38.0	35.0	40.0	23.0	41.0
38	34.83877235659662	38.0	34.0	40.0	22.0	41.0
39	34.74318491084694	38.0	34.0	40.0	21.0	41.0
40	34.653636015767	38.0	34.0	40.0	21.0	41.0
41	34.48252812816643	38.0	34.0	40.0	20.0	41.0
42	34.41053554310838	38.0	34.0	40.0	20.0	41.0
43	34.32879596024279	38.0	33.0	40.0	20.0	41.0
44	34.28712254177494	38.0	33.0	40.0	19.0	41.0
45	34.365123904499924	38.0	34.0	40.0	20.0	41.0
46	34.248056457422656	38.0	33.0	40.0	20.0	41.0
47	33.97320088089042	38.0	33.0	40.0	19.0	41.0
48	33.889471875738224	38.0	33.0	40.0	18.0	41.0
49	33.904849387652305	38.0	33.0	40.0	18.0	41.0
50	33.79047908178107	37.0	33.0	40.0	18.0	41.0
51	32.995082573715024	37.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	21.0
11	28.0
12	19.0
13	26.0
14	36.0
15	57.0
16	123.0
17	260.0
18	609.0
19	1324.0
20	2541.0
21	4429.0
22	7304.0
23	11448.0
24	18342.0
25	30554.0
26	42563.0
27	44562.0
28	41069.0
29	40340.0
30	43960.0
31	52104.0
32	65148.0
33	84102.0
34	117520.0
35	150648.0
36	185703.0
37	261640.0
38	418231.0
39	423996.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.47970457371893	3.6235208674584003	52.174112520475035	19.72266203834763
2	26.887071488396337	9.282587771788469	45.78765699157961	18.042683748235582
3	15.262178134316265	9.289518536378704	56.17082089537669	19.277482433928338
4	14.64021522464463	3.6024357244796557	60.42484610774118	21.33250294313454
5	18.34319584388404	4.218151184379814	55.66360606705466	21.77504690468149
6	17.342237250809728	8.384565675339559	58.10543157187789	16.167765501972827
7	70.74792711568911	1.0688996093391565	23.31616586198212	4.8670074129896195
8	71.71652587127521	4.453504331239786	18.40708577943769	5.422884018047321
9	66.9682200039437	3.873126009109563	19.78020690772712	9.378447079219615
10	31.853061933702843	27.255329367504284	26.60393511242481	14.287673586368065
11	20.33632755379152	22.37797461583065	37.51251930364363	19.773178526734203
12	17.583593806434482	19.439037580362704	41.48087011356692	21.49649849963589
13	19.31018392882593	20.37181111616547	43.72516882756843	16.592836127440165
14	17.42008633194653	24.77675128707227	39.16257816633444	18.640584214646754
15	14.312907426460683	24.391361729294097	42.587547270742995	18.708183573502225
16	15.947835746736194	25.759846078456256	37.38444658332829	20.90787159147926
17	16.802076886583407	26.33236628017079	36.636948979810974	20.22860785343483
18	16.891688744243073	25.149304287898104	37.443602123351994	20.515404844506833
19	17.052170110247967	27.742923298887757	35.022861761507514	20.182044829356766
20	17.757253386801093	28.421113256502718	36.246629793697494	17.5750035629987
21	18.214879082562003	28.997733347129785	35.839422969920484	16.94796460038773
22	17.071205308770445	24.17235932988292	36.56676278628451	22.189672575062133
23	16.830385643360422	26.82850164678871	35.229418069577065	21.111694640273797
24	18.297023285416696	25.71006171308977	34.711367820557626	21.281547180935906
25	16.866747753358492	29.678461331214407	32.43661278892015	21.018178126506953
26	16.55457049758985	27.309213621783297	33.98246614175073	22.153749738876122
27	18.398690768807263	26.45131186683561	33.5843376434229	21.565659720934228
28	15.719706213674497	26.955695819479942	35.167773311285046	22.156824655560523
29	16.738626224841813	25.853997098840516	34.03503257459358	23.372344101724103
30	17.260971595579147	27.40521935381846	32.788275879572595	22.545533171029795
31	17.456790099353974	28.676184916703924	30.634858036465584	23.232166947476518
32	17.123137234995873	29.515197897733152	31.432676895564114	21.928987971706864
33	16.481553428385677	25.700739346633895	30.913894523524576	26.903812701455855
34	16.819062140649617	27.102998971123114	30.558180352160935	25.519758536066334
35	17.226903471044047	26.151190236797873	30.16610407079923	26.45580222135886
36	16.994381199861774	28.577153076185695	30.530994184014727	23.897471539937797
37	16.127938009679642	27.13482191839659	33.21002754734884	23.527212524574928
38	17.483097719876067	27.519723394161367	31.530878996659567	23.466299889303
39	18.053958442745053	24.491662583047155	33.37973366340693	24.074645310800864
40	19.571942312610673	24.199545498029124	31.5913035499181	24.6372086394421
41	18.87100773317142	26.061041488923465	30.306915731664226	24.761035046240888
42	19.82267004289265	25.868005052625005	32.8814995441314	21.42782536035095
43	18.799796567416816	25.966255961921792	32.74839957907807	22.48554789158332
44	18.64024255723738	25.52053946728777	30.522208707773586	25.31700926770127
45	18.798039472168586	24.4561790206732	29.32025794158244	27.425523565575773
46	20.908750139103375	25.516390770173892	29.48581536052666	24.08904373019607
47	16.51718341536365	25.731683746283256	33.472371629549656	24.278761208803438
48	17.541277095872974	25.859512425591895	31.52111735639163	25.078093122143503
49	18.50724020858673	23.257498403971816	34.36287726299226	23.8723841244492
50	18.198967608925262	22.437715854270422	32.612419930145705	26.750896606658607
51	17.254724145807668	22.356743048247882	30.508884068807852	29.8796487371366
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	355.0
1	923.0
2	1491.0
3	30188.0
4	58885.0
5	38176.0
6	17467.0
7	17684.5
8	17902.0
9	19078.5
10	20255.0
11	21209.5
12	22164.0
13	22273.5
14	22383.0
15	22235.5
16	22088.0
17	21131.0
18	20174.0
19	19114.0
20	18054.0
21	17553.0
22	17052.0
23	16775.5
24	16499.0
25	17516.0
26	20060.5
27	21588.0
28	24132.0
29	26676.0
30	29520.5
31	32365.0
32	36359.5
33	40354.0
34	45511.5
35	50669.0
36	56666.0
37	62663.0
38	68630.5
39	74598.0
40	83077.0
41	91556.0
42	102366.0
43	113176.0
44	132400.5
45	151625.0
46	225176.5
47	298728.0
48	275669.0
49	252610.0
50	225663.5
51	198717.0
52	160440.0
53	122163.0
54	100805.5
55	79448.0
56	67310.0
57	55172.0
58	46449.0
59	37726.0
60	31840.0
61	25954.0
62	21208.0
63	16462.0
64	13626.5
65	10791.0
66	8204.5
67	5618.0
68	4098.5
69	2579.0
70	2048.5
71	1518.0
72	1326.5
73	1135.0
74	1039.5
75	663.5
76	383.0
77	271.0
78	159.0
79	120.0
80	81.0
81	65.0
82	49.0
83	34.0
84	19.0
85	11.5
86	4.0
87	3.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2048836.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.27264686427276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52387250239082	28.904985021063634
2	10.620645217779973	8.129604077816403
3	4.216961881616197	4.841828788055877
4	2.178368951459993	3.334877824772977
5	1.3652867258033827	2.6126568362576026
6	0.954078417325738	2.190906380827933
7	0.7048728570107674	1.8884144958408886
8	0.5233465753072468	1.6023886931488627
9	0.45679893022257223	1.573461372994747
>10	3.2238259816998966	23.47347845900968
>50	0.14977597648334268	3.7940828184290862
>100	0.07202414996103682	5.10662925957195
>500	0.005391860549835723	1.403498935992816
>1k	0.003081063171334699	2.9660922026260925
>5k	0.001155398689250512	2.9773437782025796
>10k+	5.135105285557831E-4	5.1997510553888935
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44017	2.148390598369025	No Hit
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	26982	1.3169428885474483	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG	17762	0.8669312721955296	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC	17068	0.8330583804657864	No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	8930	0.43585723796340947	No Hit
GAACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT	7800	0.38070397044956256	No Hit
CTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT	7635	0.37265061722851417	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	7151	0.34902744778010536	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCC	6339	0.30939518829227913	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCT	6019	0.2937765638635791	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTT	5978	0.29177542760865194	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTC	5532	0.2700069698111513	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCT	5213	0.254437153583791	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	4756	0.23213180557155377	No Hit
CCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	4689	0.22886165608179473	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTT	4109	0.200552899304776	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACATCCGTT	4029	0.196648243197601	No Hit
CGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTG	3582	0.17483097719876067	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGT	3573	0.17439170338670348	No Hit
CGTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT	3553	0.17341553935990972	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTC	3372	0.16458125491742628	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTA	3153	0.1538922588240347	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG	2878	0.14047000345562063	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC	2820	0.13763912777791876	No Hit
TTCCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT	2741	0.13378327987208347	No Hit
TCCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTG	2589	0.12636443326845098	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4404100669843756E-4	0.0	0.0	0.4005201001934757	0.0
2	2.4404100669843756E-4	0.0	0.0	1.5174957878522244	0.0
3	2.4404100669843756E-4	0.0	0.0	2.2381976888340502	0.0
4	2.4404100669843756E-4	0.0	0.0	3.6107819269087424	0.0
5	2.4404100669843756E-4	0.0	0.0	6.618294485258947	0.0
6	2.4404100669843756E-4	0.0	0.0	8.619870013998192	0.0
7	2.9284920803812506E-4	0.0	0.0	10.465844996866513	0.0
8	3.4165740937781256E-4	0.0	0.0	12.653867854723364	0.0
9	3.4165740937781256E-4	0.0	0.0	13.83863813404294	0.0
10	3.4165740937781256E-4	0.0	0.0	16.266455685081677	0.0
11	3.4165740937781256E-4	0.0	0.0	18.713259626441552	0.0
12	3.4165740937781256E-4	0.0	0.0	21.55438502642476	0.0
13	3.9046561071750006E-4	0.0	0.0	22.345468353738415	0.0
14	3.9046561071750006E-4	0.0	0.0	22.686588872901492	0.0
15	3.9046561071750006E-4	0.0	0.0	23.165494944446504	0.0
16	3.9046561071750006E-4	0.0	0.0	23.842318272423952	0.0
17	3.9046561071750006E-4	0.0	0.0	24.842691167082187	0.0
18	3.9046561071750006E-4	0.0	0.0	25.93887456097023	0.0
19	3.9046561071750006E-4	0.0	0.0	26.94290807072894	0.0
20	3.9046561071750006E-4	0.0	0.0	27.727792756472457	0.0
21	3.9046561071750006E-4	0.0	0.0	28.76403967911536	0.0
22	3.9046561071750006E-4	0.0	0.0	29.894193581135827	0.0
23	4.392738120571876E-4	0.0	0.0	30.998479136446257	0.0
24	4.392738120571876E-4	0.0	0.0	31.805620362000667	0.0
25	4.392738120571876E-4	0.0	0.0	32.487226893709405	0.0
26	4.392738120571876E-4	0.0	0.0	33.12080615529989	0.0
27	4.392738120571876E-4	0.0	0.0	33.76073048306453	0.0
28	4.392738120571876E-4	0.0	0.0	34.420324515969064	0.0
29	4.392738120571876E-4	0.0	0.0	35.11130222233502	0.0
30	4.392738120571876E-4	0.0	0.0	35.86153308512736	0.0
31	4.880820133968751E-4	0.0	0.0	36.54362769884949	0.0
32	4.880820133968751E-4	0.0	0.0	37.20961560612953	0.0
33	4.880820133968751E-4	0.0	0.0	37.842462744699915	0.0
34	4.880820133968751E-4	0.0	0.0	38.47540749967298	0.0
35	4.880820133968751E-4	0.0	0.0	39.13021832884623	0.0
36	4.880820133968751E-4	0.0	0.0	39.76936172538944	0.0
37	4.880820133968751E-4	0.0	0.0	40.392398415490554	0.0
38	4.880820133968751E-4	0.0	0.0	41.055945912703606	0.0
39	4.880820133968751E-4	0.0	0.0	41.791583123295375	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	25	3.8918784E-5	45.0	28
GCCGAAC	20	7.034581E-4	45.0	32
CGTTTTT	22825	0.0	44.457832	1
GTCCGCG	70	0.0	41.785713	1
CCGTAGT	60	3.6379788E-12	41.250004	37
GTTTGCG	305	0.0	40.573772	1
TTTGTCG	415	0.0	40.12048	1
TTACGCG	45	1.929584E-8	40.0	1
GTTTTTT	25115	0.0	39.96516	2
CCGATGA	645	0.0	39.767445	18
CTCGTTG	200	0.0	39.375	1
ACGAACG	115	0.0	39.130436	21
CGATGAA	670	0.0	38.955223	19
GCGCGAC	255	0.0	38.82353	9
CGTTTCT	795	0.0	38.773586	1
CGGGTAC	215	0.0	38.720932	6
GCGATAT	35	6.2506842E-6	38.571426	9
CGATAAC	105	0.0	38.571426	16
GCCCGCG	35	6.2506842E-6	38.571426	1
CGTTTTC	745	0.0	38.355705	1
>>END_MODULE