FastQCFastQC Report
Sat 14 Jan 2017
SRR2934405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799388
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433412.4086522751068697No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT54770.3043812674086967RNA PCR Primer, Index 4 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT53430.2969342909922707RNA PCR Primer, Index 4 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT43680.24274920139514103TruSeq Adapter, Index 4 (96% over 27bp)
CGTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC42640.23696945850478052TruSeq Adapter, Index 4 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCC41940.23307924694396095No Hit
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC38100.21173865781032217RNA PCR Primer, Index 4 (96% over 26bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGT35260.19595551376356848No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG33570.1865634315667327TruSeq Adapter, Index 4 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC29500.16394463006311036RNA PCR Primer, Index 48 (96% over 25bp)
CGTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT27840.1547192712188811TruSeq Adapter, Index 4 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTC27550.1531076121436844No Hit
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC24620.13682429803911106RNA PCR Primer, Index 48 (96% over 25bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG23060.12815468370357033No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC18930.10520243549473487No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGA207.0343516E-445.042
AGCGACG207.0343516E-445.036
CGTCCGG700.045.02
GCGTCCG253.891688E-545.01
CGGACTA253.891688E-545.038
CGTTTTT236500.044.4196621
CGTTTCT6800.040.698531
TTCGTTG5750.039.5217441
GTTTTTT265850.039.4141432
GCCGATG1600.039.3759
TGTTTCG4400.038.8636361
GCTACGA2550.038.8235310
TTCTTCG5400.038.751
CGTTTTC6200.038.467741
TTGGGCG17200.037.9360475
TTGGGAT78650.037.876675
CCTCGTC2800.037.76785736
TTCGTCG1200.037.5000041
TCTAGCG601.5643309E-1037.5000041
TTACCGG601.5643309E-1037.5000042
Produced by FastQC (version 0.11.5)