SRR2934404.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934404.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1380467
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	5185	0.3755975332985142	TruSeq Adapter, Index 10 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	5029	0.3642970096351452	RNA PCR Primer, Index 35 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4751	0.3441588969529876	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4539	0.32880177505148617	RNA PCR Primer, Index 10 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2751	0.19928038844825702	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2087	0.15118072362468643	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	2012	0.14574777955575902	TruSeq Adapter, Index 10 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1556	0.11271547961668044	No Hit

Adapter graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	35	1.2121745E-7	45.0	8
CTAACGT	20	7.033781E-4	45.0	24
CCCGTCG	25	3.8912156E-5	45.0	25
CTCGCTA	20	7.033781E-4	45.0	25
CGCAATA	20	7.033781E-4	45.0	3
TACCGAC	20	7.033781E-4	45.0	45
CGAATAT	165	0.0	40.90909	14
CGTTTTT	1635	0.0	39.908257	1
TGGGCGA	1405	0.0	39.7153	6
TACGAAT	170	0.0	39.705883	12
TAGCGAT	40	3.4588993E-7	39.375	39
CATACGG	40	3.4588993E-7	39.375	3
TATCGCG	40	3.4588993E-7	39.375	1
GCGCGAC	155	0.0	39.19355	9
CTACGTA	115	0.0	39.130432	19
CTACGAA	175	0.0	38.57143	11
TACCCGT	35	6.2492036E-6	38.571426	36
CGAGTCA	35	6.2492036E-6	38.571426	19
TTATCCG	35	6.2492036E-6	38.571426	1
ACTCGTA	35	6.2492036E-6	38.571426	44