##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934404.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1380467
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10221903167551	33.0	31.0	34.0	30.0	34.0
2	32.28666096328271	34.0	31.0	34.0	30.0	34.0
3	32.36227595444151	34.0	31.0	34.0	30.0	34.0
4	35.884574567881735	37.0	35.0	37.0	35.0	37.0
5	35.83475302198459	37.0	35.0	37.0	35.0	37.0
6	35.45953651916344	37.0	35.0	37.0	33.0	37.0
7	35.80838730661436	37.0	35.0	37.0	35.0	37.0
8	35.909273456011626	37.0	35.0	37.0	35.0	37.0
9	37.66890552255143	39.0	38.0	39.0	35.0	39.0
10	37.161821325681814	39.0	37.0	39.0	34.0	39.0
11	37.03315834424148	39.0	37.0	39.0	33.0	39.0
12	36.77403806103297	39.0	35.0	39.0	33.0	39.0
13	36.6005757471928	39.0	35.0	39.0	33.0	39.0
14	37.38506099747404	40.0	36.0	41.0	32.0	41.0
15	37.53219453996365	40.0	36.0	41.0	32.0	41.0
16	37.71074498702251	40.0	36.0	41.0	33.0	41.0
17	37.7255602633022	40.0	36.0	41.0	33.0	41.0
18	37.729721174066455	39.0	36.0	41.0	33.0	41.0
19	37.7639436509529	40.0	36.0	41.0	33.0	41.0
20	37.66213462545646	39.0	36.0	41.0	33.0	41.0
21	37.50583099777104	39.0	36.0	41.0	32.0	41.0
22	37.654525606189786	39.0	36.0	41.0	33.0	41.0
23	37.62523262055522	39.0	36.0	41.0	33.0	41.0
24	37.53514499078935	39.0	35.0	41.0	33.0	41.0
25	37.429774127161316	39.0	35.0	41.0	32.0	41.0
26	37.37053330503373	39.0	35.0	41.0	32.0	41.0
27	37.30441582449997	39.0	35.0	41.0	32.0	41.0
28	37.17602014390782	39.0	35.0	41.0	32.0	41.0
29	37.09152047821498	39.0	35.0	41.0	32.0	41.0
30	37.07411912055848	39.0	35.0	41.0	32.0	41.0
31	36.9483087969506	39.0	35.0	41.0	31.0	41.0
32	36.86679290414041	39.0	35.0	41.0	31.0	41.0
33	36.82509904257038	39.0	35.0	41.0	31.0	41.0
34	36.635198813155256	39.0	35.0	41.0	30.0	41.0
35	36.631070500055415	39.0	35.0	41.0	30.0	41.0
36	36.49409294101199	39.0	35.0	40.0	30.0	41.0
37	36.42232664743163	39.0	35.0	40.0	30.0	41.0
38	36.34777651331035	39.0	35.0	40.0	30.0	41.0
39	36.2636803342637	39.0	35.0	40.0	30.0	41.0
40	36.12593129716248	38.0	35.0	40.0	30.0	41.0
41	36.01087313206328	38.0	35.0	40.0	29.0	41.0
42	36.00778721983213	38.0	35.0	40.0	29.0	41.0
43	35.94054765525	38.0	35.0	40.0	29.0	41.0
44	35.8160760090607	38.0	35.0	40.0	29.0	41.0
45	35.88746706730404	38.0	35.0	40.0	29.0	41.0
46	35.83036102999927	38.0	35.0	40.0	29.0	41.0
47	35.613453997813785	38.0	34.0	40.0	29.0	41.0
48	35.50830407391122	38.0	34.0	40.0	28.0	41.0
49	35.54172537264564	38.0	34.0	40.0	28.0	41.0
50	35.41679083962166	38.0	34.0	40.0	28.0	41.0
51	34.40392635245899	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	22.0
10	38.0
11	45.0
12	29.0
13	25.0
14	32.0
15	51.0
16	97.0
17	165.0
18	341.0
19	639.0
20	1095.0
21	1745.0
22	2674.0
23	4008.0
24	5848.0
25	8446.0
26	11367.0
27	14392.0
28	17433.0
29	21602.0
30	26892.0
31	34719.0
32	46403.0
33	64878.0
34	102310.0
35	105295.0
36	118787.0
37	175066.0
38	286448.0
39	329457.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.877306013110058	5.062127526409541	54.43418785092291	15.62637860955749
2	20.67807488335469	4.712390806879122	52.24065479290704	22.36887951685915
3	21.379866378551608	4.912902662649668	50.67792276092076	23.029308197877967
4	19.86313327301558	5.24829640983812	49.523965440680584	25.36460487646572
5	18.17529864893547	6.034045000713527	50.09152699774786	25.69912935260314
6	22.680078553127313	6.611168539342121	52.85160746327149	17.857145444259082
7	82.78727416156995	2.7010424733079454	9.60877731955925	4.902906045562842
8	83.76274115933231	2.901988964604007	8.07719416690149	5.258075709162189
9	77.96405129568473	5.472133705477929	10.468921024551836	6.0948939742855135
10	41.244593315160735	24.059321954092347	18.91671441620843	15.779370314538484
11	29.137530994945916	24.726270167993874	26.1420229531021	19.99417588395811
12	27.69193323708571	21.031506004851984	31.227258601618146	20.04930215644416
13	27.08677570706145	21.75234902391727	31.98229294869055	19.17858232033073
14	20.925092740355254	23.30624346688476	34.54323790427442	21.225425888485564
15	18.698889578671565	24.569221864774747	35.81266339579287	20.919225160760817
16	23.787022797357707	24.09068815118362	29.981882942511483	22.14040610894719
17	24.0593943933466	24.298588810887907	28.071152733096845	23.570864062668647
18	24.47251546034784	22.857192529774345	31.90137830169066	20.768913708187156
19	25.081512270847472	24.704465952463913	28.970703392402715	21.243318384285896
20	23.469086910444073	28.42175872367829	29.172301836987046	18.936852528890586
21	23.710019870087443	24.80885091784157	32.074942754879324	19.40618645719166
22	21.854560811667355	23.17556305221349	32.1061640734621	22.863712062657058
23	21.933302281039676	23.987679531636758	31.024138932694516	23.05487925462905
24	21.901718766185645	25.698477399314868	29.114929947619174	23.28487388688031
25	23.036986758828714	25.352579960259824	26.93573986194527	24.67469341896619
26	20.081682503094967	25.69623178243305	27.730615798856473	26.49146991561551
27	19.378442222813007	24.93105593976531	31.99178249099761	23.69871934642407
28	17.460105891701865	24.339516989540495	30.387108130799216	27.813268987958423
29	19.58866093865337	25.31650521164215	30.39761182266581	24.697222027038677
30	22.62618374796355	23.70357277645898	30.81261631027761	22.85762716529986
31	21.13661536277216	24.56444087399409	30.436366823690825	23.862576939542922
32	22.891818493306975	24.078301038706467	28.71904942313	24.31083104485656
33	22.927748363416146	22.990335879090193	29.603605156805628	24.478310600688026
34	18.51033019985266	22.95904212125317	31.92057470406754	26.610052974826637
35	18.926059080006983	24.71055084982111	29.694081785366837	26.66930828480507
36	21.84470907308903	23.518852678115447	29.433879984092336	25.20255826470318
37	19.017332540364965	25.570911872576453	30.29293710027114	25.118818486787443
38	20.378393688512656	24.275770445798415	30.82724903963659	24.518586826052342
39	19.492316730497723	22.591557784430922	31.66370510848865	26.25242037658271
40	21.407683052184513	22.149750772745744	31.168148170148218	25.27441800492152
41	19.136422674355853	22.595324625652044	29.729866776967505	28.5383859230246
42	19.534693694235354	22.02066402166803	30.91620444385849	27.528437840238123
43	19.574752601836913	22.478697426305736	31.266086041897417	26.680463929959934
44	20.649026742399492	21.858472531396984	29.791005507556502	27.701495218647022
45	19.423137242686714	21.78943792209448	29.212360744588604	29.5750640906302
46	19.83343317877211	21.611237356633662	31.43494194355968	27.12038752103455
47	17.181142323576008	23.031843571776797	33.53328982148794	26.25372428315925
48	17.322905944147887	24.304239072719593	32.024959669445195	26.347895313687324
49	20.653807733180148	21.416303323440548	32.68937250944789	25.240516433931415
50	19.105925748315606	21.694107863498367	30.608772248811455	28.591194139374576
51	18.366465840907463	22.1514893148478	28.760122480291088	30.721922363953645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	265.0
1	763.5
2	1262.0
3	3029.5
4	4797.0
5	3335.5
6	1874.0
7	1914.0
8	1954.0
9	2077.5
10	2201.0
11	2433.5
12	2666.0
13	2638.0
14	2610.0
15	2591.5
16	2573.0
17	2690.0
18	2807.0
19	2824.0
20	2841.0
21	3512.0
22	4183.0
23	5134.5
24	6086.0
25	7182.5
26	10286.0
27	12293.0
28	14227.5
29	16162.0
30	18462.5
31	20763.0
32	24508.0
33	28253.0
34	31524.0
35	34795.0
36	40278.5
37	45762.0
38	49134.0
39	52506.0
40	58164.0
41	63822.0
42	70268.5
43	76715.0
44	86048.5
45	95382.0
46	109992.5
47	124603.0
48	141574.5
49	158546.0
50	151478.0
51	144410.0
52	123255.0
53	102100.0
54	92805.0
55	83510.0
56	76105.5
57	68701.0
58	63108.0
59	57515.0
60	50723.5
61	43932.0
62	37350.0
63	30768.0
64	27822.0
65	24876.0
66	21279.0
67	17682.0
68	15629.5
69	13577.0
70	11037.0
71	8497.0
72	6617.5
73	4738.0
74	3859.0
75	2395.5
76	1811.0
77	1317.0
78	823.0
79	598.5
80	374.0
81	248.0
82	122.0
83	76.0
84	30.0
85	18.0
86	6.0
87	6.0
88	6.0
89	5.0
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1380467.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.074023205201453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.45319497425226	20.059786537929412
2	11.873458863661254	6.666715193288618
3	4.67536332115304	3.9376877511239443
4	2.451949646077232	2.7534436504783084
5	1.4355313145430293	2.0150569718137175
6	0.9297319691306378	1.56607901255947
7	0.6373373817702309	1.252483711175186
8	0.475281627556267	1.0674453952816443
9	0.39252835658330737	0.9917865172277425
>10	4.4435037712007	31.36953716142241
>50	1.1294676390584781	20.237287270118216
>100	0.09481517052479277	4.853500936901859
>500	0.004962777509525394	0.9049533901236658
>1k	0.0023507893466172916	1.575253928096312
>5k	5.223976325816204E-4	0.748982572459535
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	5185	0.3755975332985142	TruSeq Adapter, Index 10 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	5029	0.3642970096351452	RNA PCR Primer, Index 35 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4751	0.3441588969529876	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4539	0.32880177505148617	RNA PCR Primer, Index 10 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2751	0.19928038844825702	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2087	0.15118072362468643	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	2012	0.14574777955575902	TruSeq Adapter, Index 10 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1556	0.11271547961668044	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.621962712618266E-4	0.0	0.0	0.4073983659153026	0.0
2	3.621962712618266E-4	0.0	0.0	1.5820008736174063	0.0
3	4.3463552551419195E-4	0.0	0.0	2.2615535177588453	0.0
4	4.3463552551419195E-4	0.0	0.0	3.0551979873477597	0.0
5	4.3463552551419195E-4	0.0	0.0	4.332736675342475	0.0
6	5.070747797665572E-4	0.0	0.0	5.1360880050012065	0.0
7	5.070747797665572E-4	0.0	0.0	5.827375808331529	0.0
8	5.070747797665572E-4	0.0	0.0	6.947793753852863	0.0
9	5.070747797665572E-4	0.0	0.0	7.486596926981956	0.0
10	5.070747797665572E-4	0.0	0.0	8.5444997960835	0.0
11	5.070747797665572E-4	0.0	0.0	10.536072213243779	0.0
12	5.070747797665572E-4	0.0	0.0	12.11452356340282	0.0
13	5.070747797665572E-4	0.0	0.0	12.636810586562374	0.0
14	5.795140340189226E-4	0.0	0.0	12.839568059214743	0.0
15	5.795140340189226E-4	0.0	0.0	13.132874599682571	0.0
16	5.795140340189226E-4	0.0	0.0	13.801923551957417	0.0
17	6.51953288271288E-4	0.0	0.0	14.780288119889864	0.0
18	6.51953288271288E-4	0.0	0.0	15.824862166208971	0.0
19	6.51953288271288E-4	0.0	0.0	16.522379745404997	0.0
20	6.51953288271288E-4	0.0	0.0	17.328338888216813	0.0
21	6.51953288271288E-4	0.0	0.0	18.426662861191176	0.0
22	6.51953288271288E-4	0.0	0.0	19.5819240880079	0.0
23	6.51953288271288E-4	0.0	0.0	20.637508900973366	0.0
24	6.51953288271288E-4	0.0	0.0	21.425792865747606	0.0
25	6.51953288271288E-4	0.0	0.0	22.130119734843355	0.0
26	7.243925425236532E-4	0.0	0.0	22.7501997512436	0.0
27	7.243925425236532E-4	0.0	0.0	23.46800032163029	0.0
28	7.243925425236532E-4	0.0	0.0	24.129153395191626	0.0
29	7.968317967760186E-4	0.0	0.0	24.925043481662364	0.0
30	7.968317967760186E-4	0.0	0.0	25.826622440087302	0.0
31	8.692710510283839E-4	0.0	0.0	26.49139747636126	0.0
32	8.692710510283839E-4	0.0	0.0	27.156969344431992	0.0
33	8.692710510283839E-4	0.0	0.0	27.83253782958955	0.0
34	8.692710510283839E-4	0.0	0.0	28.572215054760456	0.0
35	8.692710510283839E-4	0.0	0.0	29.333841373969822	0.0
36	8.692710510283839E-4	0.0	0.0	29.982172699528494	0.0
37	8.692710510283839E-4	0.0	0.0	30.637313314986883	0.0
38	9.417103052807491E-4	0.0	0.0	31.40741502694378	0.0
39	9.417103052807491E-4	0.0	0.0	32.813968026761955	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	35	1.2121745E-7	45.0	8
CTAACGT	20	7.033781E-4	45.0	24
CCCGTCG	25	3.8912156E-5	45.0	25
CTCGCTA	20	7.033781E-4	45.0	25
CGCAATA	20	7.033781E-4	45.0	3
TACCGAC	20	7.033781E-4	45.0	45
CGAATAT	165	0.0	40.90909	14
CGTTTTT	1635	0.0	39.908257	1
TGGGCGA	1405	0.0	39.7153	6
TACGAAT	170	0.0	39.705883	12
TAGCGAT	40	3.4588993E-7	39.375	39
CATACGG	40	3.4588993E-7	39.375	3
TATCGCG	40	3.4588993E-7	39.375	1
GCGCGAC	155	0.0	39.19355	9
CTACGTA	115	0.0	39.130432	19
CTACGAA	175	0.0	38.57143	11
TACCCGT	35	6.2492036E-6	38.571426	36
CGAGTCA	35	6.2492036E-6	38.571426	19
TTATCCG	35	6.2492036E-6	38.571426	1
ACTCGTA	35	6.2492036E-6	38.571426	44
>>END_MODULE