##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934402.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2199516
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.340587201911696	33.0	31.0	34.0	31.0	34.0
2	32.53251442590097	34.0	31.0	34.0	31.0	34.0
3	32.58841626976117	34.0	31.0	34.0	31.0	34.0
4	36.05637558444676	37.0	35.0	37.0	35.0	37.0
5	36.0141262896019	37.0	35.0	37.0	35.0	37.0
6	35.66463985713221	37.0	35.0	37.0	35.0	37.0
7	35.98593417824649	37.0	35.0	37.0	35.0	37.0
8	36.07975981988765	37.0	35.0	37.0	35.0	37.0
9	37.88860231069017	39.0	38.0	39.0	35.0	39.0
10	37.38712016643662	39.0	37.0	39.0	35.0	39.0
11	37.276141660256165	39.0	37.0	39.0	34.0	39.0
12	37.06931570400033	39.0	37.0	39.0	33.0	39.0
13	36.996749284842664	39.0	37.0	39.0	33.0	39.0
14	38.05457791623248	40.0	37.0	41.0	33.0	41.0
15	38.143298798462936	40.0	37.0	41.0	33.0	41.0
16	38.22049078069902	40.0	37.0	41.0	33.0	41.0
17	38.17202057179852	40.0	37.0	41.0	33.0	41.0
18	38.156283927918686	40.0	37.0	41.0	33.0	41.0
19	38.18096162974036	40.0	37.0	41.0	34.0	41.0
20	38.10289309102548	40.0	37.0	41.0	34.0	41.0
21	37.971646944145895	40.0	37.0	41.0	33.0	41.0
22	38.07030683113921	40.0	37.0	41.0	34.0	41.0
23	38.015289727376384	40.0	37.0	41.0	34.0	41.0
24	37.9689945424357	40.0	36.0	41.0	34.0	41.0
25	37.896921413620085	40.0	36.0	41.0	33.0	41.0
26	37.8409709226939	40.0	36.0	41.0	33.0	41.0
27	37.783350973577825	40.0	36.0	41.0	33.0	41.0
28	37.689111604553	40.0	36.0	41.0	33.0	41.0
29	37.61707712060289	40.0	36.0	41.0	33.0	41.0
30	37.57228317502578	40.0	36.0	41.0	33.0	41.0
31	37.497130277752014	40.0	36.0	41.0	33.0	41.0
32	37.393056017778456	40.0	35.0	41.0	32.0	41.0
33	37.33293824641421	40.0	35.0	41.0	32.0	41.0
34	37.16157736520216	39.0	35.0	41.0	32.0	41.0
35	37.09943642146727	39.0	35.0	41.0	31.0	41.0
36	36.95069551665003	39.0	35.0	41.0	31.0	41.0
37	36.8953487949167	39.0	35.0	41.0	31.0	41.0
38	36.804630200462285	39.0	35.0	41.0	31.0	41.0
39	36.7358291551414	39.0	35.0	41.0	31.0	41.0
40	36.601603261808506	39.0	35.0	41.0	30.0	41.0
41	36.510927858674364	39.0	35.0	41.0	30.0	41.0
42	36.46416620747473	39.0	35.0	41.0	30.0	41.0
43	36.3818526439453	39.0	35.0	41.0	30.0	41.0
44	36.224622598789914	38.0	35.0	40.0	30.0	41.0
45	36.24942896528145	38.0	35.0	40.0	30.0	41.0
46	36.191948592326675	38.0	35.0	40.0	30.0	41.0
47	36.00845822444574	38.0	35.0	40.0	30.0	41.0
48	35.913358666179285	38.0	35.0	40.0	30.0	41.0
49	35.891448846018854	38.0	35.0	40.0	29.0	41.0
50	35.78105137675743	38.0	34.0	40.0	29.0	41.0
51	34.82903602428898	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	37.0
10	39.0
11	45.0
12	38.0
13	30.0
14	41.0
15	79.0
16	116.0
17	224.0
18	387.0
19	743.0
20	1358.0
21	2203.0
22	3403.0
23	5052.0
24	7377.0
25	11091.0
26	15539.0
27	19416.0
28	22942.0
29	27490.0
30	34143.0
31	43918.0
32	58772.0
33	83924.0
34	142649.0
35	159874.0
36	180726.0
37	272425.0
38	467900.0
39	637303.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.233975110888032	4.199242015061495	55.9967738356984	16.570009038352072
2	19.87750941570782	4.486441562598317	53.65366744320114	21.982381578492724
3	20.751383486185144	4.704398604056529	52.35169919200406	22.192518717754268
4	18.904568095890188	4.855795547747777	50.76616855708256	25.473467799279476
5	18.419324978768056	5.534581244237369	51.30501437588997	24.741079401104606
6	21.55551494055965	6.257376622857029	53.304908898139416	18.88219953844391
7	85.95354614378799	1.9729795100376628	8.12060471485545	3.9528696313188902
8	88.76998394192177	2.3430609279496037	5.849104984914863	3.037850145213765
9	83.91423385872164	4.428565193433465	7.438954751863591	4.218246195981298
10	40.01898599510074	28.91977144062603	15.787746031399635	15.273496532873596
11	29.214563567621237	25.21113735930996	25.772624522849576	19.801674550219232
12	26.296785292764408	23.303581333347882	29.478257944020413	20.921375429867297
13	24.95198943767629	22.994467873841337	30.948535950636412	21.10500673784596
14	21.728280221648763	24.601457775256012	31.9061557178943	21.764106285200928
15	20.866408791752367	24.119260782826768	32.95374982496149	22.060580600459375
16	24.799364951198356	24.635874437830868	29.470710829109674	21.094049781861102
17	25.02605118580633	24.543672335186468	28.248078213570622	22.18219826543658
18	25.582810036389823	23.354865343102755	29.523586098032478	21.538738522474947
19	25.300111479070853	24.990043264063548	28.00670693007007	21.703138326795532
20	26.0770096694	27.395936196872402	27.548515218802685	18.97853891492492
21	25.802858447040165	26.05955128310046	29.235840975923793	18.901749293935573
22	25.154533997479444	22.43079841201428	30.23937993631326	22.175287654193014
23	23.513445685323497	24.52489547700494	29.725721476906735	22.235937360764822
24	23.213470599895615	25.337437872695627	29.65106868965718	21.798022837751578
25	23.582870049592728	26.708603165423668	26.801532700830542	22.90699408415306
26	22.204657751978164	26.746656991810923	27.181889106512525	23.866796149698388
27	21.5790201116973	25.767168777130966	30.143949850785358	22.509861260386376
28	19.628591017296532	26.423676845269593	29.888939202988297	24.058792934445577
29	21.422121957739794	25.321207029182784	30.21787520527243	23.03879580780499
30	23.429154413971073	24.176818900158032	30.69638956934162	21.697637116529272
31	23.90585019613406	25.839821124283706	28.60292900801813	21.651399671564107
32	23.867296259722593	25.071697591651983	28.535459619298067	22.52554652932736
33	24.019375171628667	24.9846329828926	27.411666930361044	23.58432491511769
34	21.997566737409503	24.93953215161881	30.036335266485896	23.026565844485784
35	21.678223754680577	26.033772884580063	29.396876403717908	22.891126957021456
36	23.484666626657866	26.39603440029534	28.34614524286252	21.773153730184276
37	23.004606467968408	26.646225806040963	28.24816914266593	22.100998583324696
38	23.222381651236	26.244319204770505	28.304999827234717	22.22829931675878
39	22.370466957276054	24.84901223723765	27.510734179701352	25.269786625784945
40	23.87243375360761	25.11093349627827	27.925870964339428	23.09076178577469
41	22.762962397181926	25.468148447203838	27.187117529492856	24.58177162612138
42	23.92790050174675	24.454971002711506	28.5140912819002	23.103037213641546
43	23.507171577747105	23.634608704824153	29.13459142829604	23.7236282891327
44	23.354274303983242	24.20200625955892	28.638209496998428	23.80550993945941
45	22.778829524313533	23.7141261986728	27.795569570760115	25.711474706253558
46	22.55832646818664	24.057519927111237	28.72568328668671	24.658470318015418
47	20.933741786829465	25.576899645194672	30.615644532706288	22.873714035269575
48	21.929415380474612	25.721113190356427	29.9260382738748	22.423433155294166
49	23.730766223114543	23.95267868021874	29.36559679493125	22.950958301735472
50	21.870129610332455	24.490751601716013	28.768829142411327	24.8702896455402
51	21.5292818965627	24.26742974363451	27.901865683177572	26.30142267662522
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	286.0
1	1165.5
2	2045.0
3	6151.5
4	10258.0
5	7009.0
6	3760.0
7	3783.0
8	3806.0
9	3982.0
10	4158.0
11	4194.5
12	4231.0
13	4243.0
14	4255.0
15	4360.0
16	4465.0
17	4665.0
18	4865.0
19	5223.5
20	5582.0
21	5851.0
22	6120.0
23	7804.0
24	9488.0
25	12187.5
26	17410.5
27	19934.0
28	23744.0
29	27554.0
30	32112.5
31	36671.0
32	44256.5
33	51842.0
34	56305.0
35	60768.0
36	66214.0
37	71660.0
38	77928.0
39	84196.0
40	92787.0
41	101378.0
42	109609.0
43	117840.0
44	128782.0
45	139724.0
46	155881.5
47	172039.0
48	187024.5
49	202010.0
50	204814.5
51	207619.0
52	188619.0
53	169619.0
54	156668.5
55	143718.0
56	134799.0
57	125880.0
58	115299.0
59	104718.0
60	94179.0
61	83640.0
62	73487.0
63	63334.0
64	55669.5
65	48005.0
66	40011.5
67	32018.0
68	26914.0
69	21810.0
70	18325.5
71	14841.0
72	11255.5
73	7670.0
74	7098.0
75	4923.0
76	3320.0
77	2450.0
78	1580.0
79	1220.5
80	861.0
81	591.5
82	322.0
83	185.5
84	49.0
85	83.0
86	117.0
87	77.5
88	38.0
89	21.5
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2199516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.58203582628613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.94283298583495	17.685012979023142
2	11.35340716837421	5.581797235267772
3	4.180445919145609	3.0829161406286705
4	2.072857273720935	2.0382020706154336
5	1.199154406340822	1.4738828288959482
6	0.7757782445980448	1.14421251611775
7	0.5602592767235903	0.9640619528699916
8	0.43304035493685705	0.8516010815428385
9	0.35445838071016067	0.7841977752190086
>10	5.440560554105849	35.37920858526149
>50	1.5934397051457945	25.151127478458417
>100	0.08757722852498921	3.8600958847248985
>500	0.0035630768159732786	0.6159257991725925
>1k	0.0024378946635606642	1.118356402060257
>5k	1.8753035873543571E-4	0.2694012701418031
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5844	0.26569481649599275	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	3810	0.17321992656566262	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	3229	0.14680502437809045	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2889	0.1313470781753804	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2500	0.11366136913757391	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3639364296508868E-4	0.0	0.0	0.15744372852936736	0.0
2	1.3639364296508868E-4	0.0	0.0	0.6215003664442541	0.0
3	1.3639364296508868E-4	0.0	0.0	0.9223392782775847	0.0
4	1.8185819062011823E-4	0.0	0.0	1.2916477988793897	0.0
5	1.8185819062011823E-4	0.0	0.0	1.959294681193499	0.0
6	1.8185819062011823E-4	0.0	0.0	2.460768641828475	0.0
7	2.273227382751478E-4	0.0	0.0	2.8984103775557895	0.0
8	2.273227382751478E-4	0.0	0.0	3.519092382142253	0.0
9	2.273227382751478E-4	0.0	0.0	3.836298531131394	0.0
10	2.273227382751478E-4	0.0	0.0	4.497262124940214	0.0
11	2.273227382751478E-4	0.0	0.0	5.766223114539744	0.0
12	2.273227382751478E-4	0.0	0.0	6.831184678811157	0.0
13	2.273227382751478E-4	0.0	0.0	7.174942123630835	0.0
14	2.273227382751478E-4	0.0	0.0	7.307789531878831	0.0
15	2.273227382751478E-4	0.0	0.0	7.503969055010284	0.0
16	2.7278728593017735E-4	0.0	0.0	7.931199409324597	0.0
17	2.7278728593017735E-4	0.0	0.0	8.52928553372651	0.0
18	2.7278728593017735E-4	0.0	0.0	9.163334115323552	0.0
19	2.7278728593017735E-4	0.0	0.0	9.646304005062932	0.0
20	2.7278728593017735E-4	0.0	0.0	10.16587285566461	0.0
21	2.7278728593017735E-4	0.0	0.0	10.893260153597428	0.0
22	2.7278728593017735E-4	0.0	0.0	11.68043333169661	0.0
23	2.7278728593017735E-4	0.0	0.0	12.39536334357195	0.0
24	2.7278728593017735E-4	0.0	0.0	12.968489431311252	0.0
25	2.7278728593017735E-4	0.0	0.0	13.461370592439428	0.0
26	2.7278728593017735E-4	0.0	0.0	13.926973024974584	0.0
27	2.7278728593017735E-4	0.0	0.0	14.423445885367508	0.0
28	2.7278728593017735E-4	0.0	0.0	14.909552828895084	0.0
29	2.7278728593017735E-4	0.0	0.0	15.448307718607184	0.0
30	2.7278728593017735E-4	0.0	0.0	16.078173561819963	0.0
31	3.182518335852069E-4	0.0	0.0	16.58364840264858	0.0
32	3.182518335852069E-4	0.0	0.0	17.078029893849376	0.0
33	3.182518335852069E-4	0.0	0.0	17.576184942505535	0.0
34	3.6371638124023647E-4	0.0	0.0	18.100709428801608	0.0
35	3.6371638124023647E-4	0.0	0.0	18.67706349942442	0.0
36	4.09180928895266E-4	0.0	0.0	19.17544587081885	0.0
37	4.09180928895266E-4	0.0	0.0	19.697788058827488	0.0
38	4.09180928895266E-4	0.0	0.0	20.273596554878438	0.0
39	4.09180928895266E-4	0.0	0.0	21.124010918765766	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGGT	50	1.0822987E-9	40.5	40
AGTACGC	85	0.0	39.705883	17
CGTTTTT	3410	0.0	39.325516	1
CACGACC	980	0.0	39.030613	27
TTTGGGA	12865	0.0	38.93121	4
AACACGT	955	0.0	38.874348	41
TCGTTCA	250	0.0	38.7	16
TAAGCGA	70	0.0	38.571426	19
TTGGGAT	7125	0.0	38.463158	5
CGTGCGG	390	0.0	38.076923	2
TACGGGA	315	0.0	37.857143	4
TTGGGAC	5015	0.0	37.821537	5
TTGTTCG	530	0.0	37.78302	1
TTTGGGC	5795	0.0	37.467648	4
CGACCAA	1015	0.0	37.463055	29
TCTTGCG	790	0.0	37.310127	1
TGGGCGA	3000	0.0	37.274998	6
TCAAGCG	1075	0.0	37.255814	17
GCGATGT	535	0.0	37.009346	9
AGACACG	1060	0.0	36.933964	24
>>END_MODULE