SRR2934400.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934400.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1443699
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC	14943	1.0350495498022787	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG	14205	0.9839308609343083	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC	13258	0.9183354702053546	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	6690	0.4633929925836341	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	5878	0.4071485815256504	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	5056	0.35021150530685413	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	4636	0.32111956855272467	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT	3654	0.25309984976092664	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3515	0.24347180402563137	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT	3441	0.2383460818356181	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC	3294	0.22816390397167274	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT	2892	0.20031876450700595	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTA	2659	0.18417966626007223	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTG	1948	0.13493117332629587	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	20	7.033888E-4	45.0	8
CAACGGT	20	7.033888E-4	45.0	27
CGATTCA	25	3.8913036E-5	45.0	10
TCAATCG	20	7.033888E-4	45.0	33
CTACGTC	35	1.2122109E-7	45.0	11
ACTACGT	35	1.2122109E-7	45.0	10
ACCGTTC	25	3.8913036E-5	45.0	45
CCGCGTA	25	3.8913036E-5	45.0	34
TATCGGC	20	7.033888E-4	45.0	22
GCCCGTA	25	3.8913036E-5	45.0	31
CGATGAA	555	0.0	41.351353	19
CTACGGC	285	0.0	41.05263	6
CGAACGA	55	6.184564E-11	40.90909	22
CCGATGA	575	0.0	40.695652	18
CGTTTGG	1070	0.0	40.16355	2
TCGTTTG	590	0.0	40.042374	1
TTTCGCG	360	0.0	40.0	1
TTTGTCG	330	0.0	39.545452	1
TACGGCT	1940	0.0	39.43299	7
GCGTTTA	80	0.0	39.375	11