##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934400.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1443699 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03967170442038 33.0 31.0 34.0 30.0 34.0 2 32.20983667648173 34.0 31.0 34.0 30.0 34.0 3 32.30293087409495 34.0 31.0 34.0 30.0 34.0 4 35.85358374564227 37.0 35.0 37.0 35.0 37.0 5 35.80279338006053 37.0 35.0 37.0 35.0 37.0 6 35.311902965922954 37.0 35.0 37.0 33.0 37.0 7 35.77662379761986 37.0 35.0 37.0 35.0 37.0 8 35.78706641758428 37.0 35.0 37.0 35.0 37.0 9 37.62031628476573 39.0 37.0 39.0 35.0 39.0 10 37.112249159970325 39.0 37.0 39.0 33.0 39.0 11 37.01704371894696 39.0 37.0 39.0 33.0 39.0 12 36.810848383215614 39.0 35.0 39.0 33.0 39.0 13 36.68223500882109 39.0 35.0 39.0 33.0 39.0 14 37.63846203398354 40.0 36.0 41.0 32.0 41.0 15 37.648108781678175 40.0 36.0 41.0 32.0 41.0 16 37.797957191907734 40.0 36.0 41.0 33.0 41.0 17 37.710429944191965 40.0 36.0 41.0 33.0 41.0 18 37.70714324800391 39.0 36.0 41.0 33.0 41.0 19 37.772985920195275 40.0 36.0 41.0 33.0 41.0 20 37.63972822589751 39.0 36.0 41.0 32.0 41.0 21 37.537252571346244 39.0 36.0 41.0 32.0 41.0 22 37.677276218934836 39.0 36.0 41.0 33.0 41.0 23 37.613194301582254 39.0 36.0 41.0 33.0 41.0 24 37.5762246839542 39.0 36.0 41.0 33.0 41.0 25 37.5185984059004 39.0 36.0 41.0 33.0 41.0 26 37.45573211590504 39.0 36.0 41.0 33.0 41.0 27 37.315283171907716 39.0 35.0 41.0 32.0 41.0 28 37.20697874002822 39.0 35.0 41.0 32.0 41.0 29 37.1285891311139 39.0 35.0 41.0 32.0 41.0 30 37.08107645707312 39.0 35.0 41.0 31.0 41.0 31 37.06444972255297 39.0 35.0 41.0 31.0 41.0 32 36.9022303125513 39.0 35.0 41.0 31.0 41.0 33 36.88403954009804 39.0 35.0 41.0 31.0 41.0 34 36.73522735694906 39.0 35.0 41.0 31.0 41.0 35 36.646261443694286 39.0 35.0 41.0 30.0 41.0 36 36.49009939052392 39.0 35.0 40.0 30.0 41.0 37 36.37807673206118 39.0 35.0 40.0 30.0 41.0 38 36.36588513256572 39.0 35.0 40.0 30.0 41.0 39 36.34006811669192 39.0 35.0 40.0 30.0 41.0 40 36.25764581121134 39.0 35.0 40.0 30.0 41.0 41 36.15629504488124 39.0 35.0 40.0 30.0 41.0 42 36.10883916938364 38.0 35.0 40.0 30.0 41.0 43 36.04151834973911 38.0 35.0 40.0 29.0 41.0 44 35.94347783021253 38.0 35.0 40.0 29.0 41.0 45 36.01001732355567 38.0 35.0 40.0 29.0 41.0 46 35.892653524037904 38.0 35.0 40.0 29.0 41.0 47 35.585372020067894 38.0 34.0 40.0 28.0 41.0 48 35.519026472969784 38.0 34.0 40.0 28.0 41.0 49 35.56039728502964 38.0 34.0 40.0 28.0 41.0 50 35.45280214227481 38.0 34.0 40.0 28.0 41.0 51 34.489435817299864 37.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 10.0 10 27.0 11 19.0 12 16.0 13 24.0 14 18.0 15 34.0 16 60.0 17 153.0 18 330.0 19 620.0 20 1198.0 21 2046.0 22 3373.0 23 5006.0 24 6625.0 25 8848.0 26 11694.0 27 14339.0 28 17242.0 29 21648.0 30 27357.0 31 36041.0 32 46947.0 33 65409.0 34 102570.0 35 112550.0 36 126495.0 37 183172.0 38 300033.0 39 349691.0 40 103.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.154552992001797 4.376258486014051 52.73571568588743 14.73347283609672 2 18.561140514747187 9.91383938064652 50.34899934127544 21.17602076333086 3 19.79256063763984 9.982066898986561 48.9955316170476 21.229840846326002 4 18.1903568541642 4.441369011130437 54.02538894880442 23.34288518590094 5 21.80108180444816 5.436313248121665 47.805255804707215 24.957349142722965 6 20.74698396272353 9.900124610462429 52.113771637993786 17.239119788820247 7 79.7881691405203 1.9987545880408588 13.490485205018498 4.722591066420355 8 80.41419991286273 6.161672204524627 8.306579141496947 5.117548741115704 9 75.29623557265053 4.647229096923943 10.087144203881834 9.969391126543691 10 40.282565825701894 28.04559676220597 17.286082486723338 14.385754925368794 11 28.75696388236052 22.9452953835945 28.497352980087953 19.800387753957022 12 25.55283338147356 20.365325459115784 32.42026211835016 21.6615790410605 13 24.93289806254628 20.67155272671104 35.1927236910187 19.202825519723987 14 20.779608491797806 25.279507709016908 32.49534702178224 21.445536777403046 15 18.11728067969847 23.712006450097977 37.692482989875316 20.478229880328243 16 21.842641714096914 24.261844054751023 30.16286635926187 23.732647871890194 17 21.58815653401436 24.510303047934507 30.53531241623081 23.366228001820325 18 21.820753495015236 23.313100583985996 32.139317129124564 22.72682879187421 19 22.515635184342443 25.293638078297487 29.611781957319362 22.578944780040715 20 23.29342889341892 27.36858583402773 31.41222650981957 17.925758762733786 21 23.885519072881536 27.816740193073485 30.478998738656742 17.818741995388233 22 21.423994890901774 22.661926066306066 32.088336973288754 23.82574206950341 23 20.982213051335492 26.240303553580073 30.382718281303788 22.394765113780643 24 22.468118354310697 24.198118859956267 29.048922247643034 24.284840538090002 25 21.513002364066196 27.91121972100833 26.597857309591543 23.97792060533394 26 20.444566353512748 25.179833192375973 28.73168160399086 25.643918850120418 27 21.777184856400122 25.906092613487992 29.705638086609465 22.61108444350242 28 18.523113197418574 25.94723692404026 31.336864540323155 24.19278533821801 29 22.512033325506216 24.730016436944265 29.353417852336257 23.404532385213262 30 22.654583815601452 24.974319439162873 30.224582825090273 22.146513920145406 31 23.97154808585446 25.118670858676218 26.93296871439268 23.976812341076638 32 23.616557190938 27.46645942125055 26.91537501930804 22.001608368503405 33 22.125733965321025 24.30215716711032 27.13522694134996 26.436881926218692 34 22.57859844746031 23.71976429989908 28.965386829249034 24.736250423391578 35 20.73153752963741 25.676612645710772 29.01796011495471 24.573889709697106 36 24.28691853357244 26.490424943149506 27.110775861173273 22.111880662104774 37 21.090476615970505 26.981524542165648 30.51224666637575 21.415752175488105 38 21.98768579877107 27.029803303874285 27.017543130527898 23.964967766826742 39 21.267452564558127 26.470060587421617 27.02578584594157 25.23670100207869 40 20.704385055333557 22.920013105224843 30.13135009444489 26.244251744996706 41 19.291417393791917 25.258381421612125 28.11638714164102 27.333814042954934 42 20.285668965622335 23.886488804106673 31.950635139319207 23.877207090951785 43 21.95339887331085 24.750311526156075 28.73119673837829 24.565092862154785 44 21.95526906924504 23.07856416053485 28.801848584781176 26.164318185438933 45 21.763539352732113 21.703623816321823 27.794090042314917 28.738746788631147 46 23.423372877587365 24.075863459072842 28.59307930531226 23.907684358027538 47 18.040810445944757 23.521731330422753 34.229849850973096 24.207608372659397 48 19.94910296398349 24.942110509185085 30.187386705954633 24.92139982087679 49 21.405500731108077 21.963581051174792 32.72607378684892 23.90484443086821 50 20.95471424445123 22.017262601137773 29.521596953381557 27.506426201029438 51 19.702098567637712 21.93850657235338 27.84998812079249 30.509406739216416 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 272.0 1 876.0 2 1480.0 3 3684.0 4 5888.0 5 3911.0 6 1934.0 7 2003.5 8 2073.0 9 2264.5 10 2456.0 11 2645.5 12 2835.0 13 2955.5 14 3076.0 15 2951.5 16 2827.0 17 2740.0 18 2653.0 19 3264.5 20 3876.0 21 4603.5 22 5331.0 23 6434.0 24 7537.0 25 8851.5 26 12073.5 27 13981.0 28 16108.0 29 18235.0 30 21471.5 31 24708.0 32 28403.0 33 32098.0 34 35500.0 35 38902.0 36 41335.5 37 43769.0 38 48498.0 39 53227.0 40 58857.0 41 64487.0 42 70196.5 43 75906.0 44 85531.5 45 95157.0 46 134648.5 47 174140.0 48 168051.0 49 161962.0 50 153201.5 51 144441.0 52 124120.0 53 103799.0 54 92770.0 55 81741.0 56 74147.5 57 66554.0 58 61484.5 59 56415.0 60 50480.5 61 44546.0 62 38099.0 63 31652.0 64 27783.5 65 23915.0 66 19801.5 67 15688.0 68 12996.0 69 10304.0 70 8168.5 71 6033.0 72 5263.5 73 4494.0 74 3496.0 75 1813.5 76 1129.0 77 985.0 78 841.0 79 583.5 80 326.0 81 258.0 82 190.0 83 146.0 84 102.0 85 59.5 86 17.0 87 14.0 88 11.0 89 17.0 90 23.0 91 13.0 92 3.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1443699.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.21310596500936 #Duplication Level Percentage of deduplicated Percentage of total 1 70.0931668614115 18.37359610361262 2 12.202431446250092 6.3972725706263205 3 5.186759900824984 4.078832606861601 4 2.6443821929632363 2.7726988254451657 5 1.5737883407335167 2.0626940271071965 6 0.9882790899935674 1.554351870540264 7 0.6938823632645048 1.2732168340852508 8 0.5091081866783976 1.0676245476043682 9 0.39158835438251216 0.9238272325283184 >10 4.419353401016006 29.2738608158139 >50 1.1875380694421116 20.328241001886642 >100 0.09880287676974087 4.428850580652769 >500 0.005592615650390142 0.958623247508174 >1k 0.003728410433593428 2.3156489763394728 >5k 7.989450929128775E-4 1.2303215929277642 >10k+ 7.989450929128775E-4 2.9603391664602117 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC 14943 1.0350495498022787 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG 14205 0.9839308609343083 No Hit GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC 13258 0.9183354702053546 No Hit GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC 6690 0.4633929925836341 TruSeq Adapter, Index 16 (95% over 22bp) CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT 5878 0.4071485815256504 TruSeq Adapter, Index 13 (95% over 22bp) TCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC 5056 0.35021150530685413 TruSeq Adapter, Index 16 (95% over 22bp) CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC 4636 0.32111956855272467 TruSeq Adapter, Index 16 (95% over 22bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT 3654 0.25309984976092664 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3515 0.24347180402563137 No Hit GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT 3441 0.2383460818356181 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC 3294 0.22816390397167274 No Hit GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT 2892 0.20031876450700595 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTA 2659 0.18417966626007223 No Hit TCCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTG 1948 0.13493117332629587 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.0779954824378213E-4 0.0 0.0 0.44268230427533717 0.0 2 2.0779954824378213E-4 0.0 0.0 1.7094976168855143 0.0 3 2.0779954824378213E-4 0.0 0.0 2.3660056563037033 0.0 4 2.0779954824378213E-4 0.0 0.0 3.3368451456986534 0.0 5 2.0779954824378213E-4 0.0 0.0 5.49262692569573 0.0 6 2.0779954824378213E-4 0.0 0.0 6.259130192650961 0.0 7 2.0779954824378213E-4 0.0 0.0 7.193604761103249 0.0 8 2.0779954824378213E-4 0.0 0.0 8.457718679586257 0.0 9 2.0779954824378213E-4 0.0 0.0 8.94306915776765 0.0 10 2.0779954824378213E-4 0.0 0.0 10.97424047533454 0.0 11 2.0779954824378213E-4 0.0 0.0 12.871311817768108 0.0 12 2.0779954824378213E-4 0.0 0.0 15.706182521425864 0.0 13 2.0779954824378213E-4 0.0 0.0 16.300073630306596 0.0 14 2.770660643250428E-4 0.0 0.0 16.545831229362907 0.0 15 2.770660643250428E-4 0.0 0.0 17.023493124259282 0.0 16 2.770660643250428E-4 0.0 0.0 17.726409729451916 0.0 17 2.770660643250428E-4 0.0 0.0 18.68699777446684 0.0 18 2.770660643250428E-4 0.0 0.0 19.69150079067728 0.0 19 3.463325804063035E-4 0.0 0.0 20.727589338220778 0.0 20 3.463325804063035E-4 0.0 0.0 21.53364378585841 0.0 21 3.463325804063035E-4 0.0 0.0 22.54403445593576 0.0 22 3.463325804063035E-4 0.0 0.0 23.68409204411723 0.0 23 4.1559909648756425E-4 0.0 0.0 24.689772591101054 0.0 24 4.1559909648756425E-4 0.0 0.0 25.47567048255904 0.0 25 4.1559909648756425E-4 0.0 0.0 26.154136007574987 0.0 26 4.1559909648756425E-4 0.0 0.0 26.758971225996554 0.0 27 4.1559909648756425E-4 0.0 0.0 27.382646936792227 0.0 28 4.1559909648756425E-4 0.0 0.0 28.042964634594885 0.0 29 4.1559909648756425E-4 0.0 0.0 28.72946507547626 0.0 30 4.1559909648756425E-4 0.0 0.0 29.499016069139067 0.0 31 4.1559909648756425E-4 0.0 0.0 30.19438262407884 0.0 32 4.1559909648756425E-4 0.0 0.0 30.816742271068968 0.0 33 4.1559909648756425E-4 0.0 0.0 31.459189207722662 0.0 34 4.8486561256882494E-4 0.0 0.0 32.1073852652111 0.0 35 5.541321286500856E-4 0.0 0.0 32.779478270747575 0.0 36 5.541321286500856E-4 0.0 0.0 33.38646075116766 0.0 37 5.541321286500856E-4 0.0 0.0 34.008889664673866 0.0 38 5.541321286500856E-4 0.0 0.0 34.74907165551822 0.0 39 5.541321286500856E-4 0.0 0.0 35.76625044417153 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACGT 20 7.033888E-4 45.0 8 CAACGGT 20 7.033888E-4 45.0 27 CGATTCA 25 3.8913036E-5 45.0 10 TCAATCG 20 7.033888E-4 45.0 33 CTACGTC 35 1.2122109E-7 45.0 11 ACTACGT 35 1.2122109E-7 45.0 10 ACCGTTC 25 3.8913036E-5 45.0 45 CCGCGTA 25 3.8913036E-5 45.0 34 TATCGGC 20 7.033888E-4 45.0 22 GCCCGTA 25 3.8913036E-5 45.0 31 CGATGAA 555 0.0 41.351353 19 CTACGGC 285 0.0 41.05263 6 CGAACGA 55 6.184564E-11 40.90909 22 CCGATGA 575 0.0 40.695652 18 CGTTTGG 1070 0.0 40.16355 2 TCGTTTG 590 0.0 40.042374 1 TTTCGCG 360 0.0 40.0 1 TTTGTCG 330 0.0 39.545452 1 TACGGCT 1940 0.0 39.43299 7 GCGTTTA 80 0.0 39.375 11 >>END_MODULE