FastQCFastQC Report
Sat 14 Jan 2017
SRR2934396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814745
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277080.9843875732970482No Hit
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT120380.42767639697379334Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC114640.40728378592021663Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC98150.34869943813738014No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC76280.2710014583914351Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC59660.21195525704815177Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC36700.13038481283384462No Hit
TCCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG36600.13002954086427013No Hit
TTCCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT35600.12647682116852504No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG28840.10246043602528826No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACG253.8922517E-545.022
CGTTTTT136850.043.717571
TATCGCG650.041.538461
CTATGCG501.0822987E-940.51
AGGCACG2750.040.09090810
TTTCGCG8750.039.3428541
TATTGCG1500.039.01
ACGCCTA356.2515173E-638.57142612
TTTGGGA208000.038.254
TTCGTCG2250.038.01
TTGGGAT120250.037.590445
GCCGATT4400.037.3295449
TTGGGAC80950.037.3285985
TTCTACG1450.037.241381
GGCCGAT8300.037.1385548
TGCGGGC9950.036.85934
GTTTTTT165400.036.525092
TTGGGAA103050.036.222715
CGTTTGG16100.036.1956522
TGGGCGA36950.036.17056
Produced by FastQC (version 0.11.5)