FastQCFastQC Report
Sat 14 Jan 2017
SRR2934394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151324
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371811.7282845354767575No Hit
CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT65010.30218600266626505No Hit
CGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTG57690.26816044445188175No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT56690.26351214414937035No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTC54400.25286753645661925No Hit
CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC51950.24147920071546639No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT50020.2325079811316194No Hit
TCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC42830.19908670195656256No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCC41980.1951356466994279No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC34720.16138898650319525No Hit
CGTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCT30590.14219150625382324No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTC25420.11815979368983937No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGT24420.113511493387328No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAT207.0346595E-445.018
CGTTTTT240750.044.271031
GTTTTTT274350.039.0541272
TGGGCGA26550.038.813566
CGTTAGT301.1402529E-437.49999616
TTCGTTG9400.037.1010631
TATTGCG850.037.0588231
TTGGGAC45500.036.989015
TCTTGCG6450.036.9767461
TTTCGCG5550.036.891891
CGTTCTG8550.036.8421061
TTTGGGC48150.036.7757034
GGCGATT24250.036.7422648
TTGGGCG21150.036.702135
TTGGGAT73300.036.6814465
GTTGGGA36400.036.655224
GGGCGAT45200.036.587397
GCGATTG6150.036.5853659
GCGGGCG4800.036.56255
CGTTTTC7050.036.3829771
Produced by FastQC (version 0.11.5)