##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934394.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2151324
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0842332442719	33.0	31.0	34.0	30.0	34.0
2	32.3247772069665	34.0	31.0	34.0	30.0	34.0
3	32.284883169620194	34.0	31.0	34.0	30.0	34.0
4	35.89203811234384	37.0	35.0	37.0	35.0	37.0
5	35.89696298651435	37.0	35.0	37.0	35.0	37.0
6	35.591463675392454	37.0	35.0	37.0	33.0	37.0
7	35.92334673903141	37.0	35.0	37.0	35.0	37.0
8	36.00673399264825	37.0	35.0	37.0	35.0	37.0
9	37.79884433957879	39.0	38.0	39.0	35.0	39.0
10	37.26148641487754	39.0	37.0	39.0	34.0	39.0
11	37.163424012375636	39.0	37.0	39.0	34.0	39.0
12	37.08284805078175	39.0	37.0	39.0	33.0	39.0
13	37.08567793600592	39.0	37.0	39.0	33.0	39.0
14	38.31194464432136	40.0	38.0	41.0	33.0	41.0
15	38.32134722617328	40.0	38.0	41.0	33.0	41.0
16	38.309956101451945	40.0	38.0	41.0	33.0	41.0
17	38.2422127025032	40.0	38.0	41.0	33.0	41.0
18	38.059444323588636	39.0	37.0	41.0	33.0	41.0
19	37.917105001385195	39.0	37.0	41.0	34.0	41.0
20	37.71321428106599	39.0	35.0	41.0	33.0	41.0
21	37.553943060180615	39.0	35.0	41.0	33.0	41.0
22	37.62976427539506	39.0	35.0	41.0	33.0	41.0
23	37.569805849792964	39.0	35.0	41.0	33.0	41.0
24	37.50668007236474	39.0	35.0	41.0	33.0	41.0
25	37.44120550879366	39.0	35.0	41.0	33.0	41.0
26	37.37462279043045	39.0	35.0	41.0	33.0	41.0
27	37.29020175482633	39.0	35.0	41.0	33.0	41.0
28	37.14803349007402	39.0	35.0	41.0	32.0	41.0
29	37.02818125024404	39.0	35.0	41.0	32.0	41.0
30	36.904466737692694	39.0	35.0	41.0	31.0	41.0
31	36.70277559307664	39.0	35.0	41.0	31.0	41.0
32	36.325116532888586	39.0	35.0	41.0	30.0	41.0
33	35.90764338611943	39.0	35.0	41.0	28.0	41.0
34	35.51199726308078	39.0	35.0	41.0	25.0	41.0
35	35.264386024606246	39.0	35.0	40.0	23.0	41.0
36	35.00999756429064	38.0	35.0	40.0	22.0	41.0
37	34.94625309809215	38.0	35.0	40.0	22.0	41.0
38	34.87738806428042	38.0	34.0	40.0	21.0	41.0
39	34.76810977797859	38.0	34.0	40.0	21.0	41.0
40	34.681762021899075	38.0	34.0	40.0	21.0	41.0
41	34.58907258971684	38.0	34.0	40.0	20.0	41.0
42	34.46325936957892	38.0	34.0	40.0	19.0	41.0
43	34.377477776476255	38.0	34.0	40.0	18.0	41.0
44	34.23888126567639	38.0	33.0	40.0	18.0	41.0
45	34.253057651938995	38.0	34.0	40.0	19.0	41.0
46	34.14251316863476	38.0	33.0	40.0	19.0	41.0
47	33.93648004670612	38.0	33.0	40.0	18.0	41.0
48	33.800723647391095	38.0	33.0	40.0	18.0	41.0
49	33.760981144634655	37.0	33.0	40.0	18.0	41.0
50	33.63852167316499	37.0	33.0	40.0	18.0	41.0
51	32.79459858208248	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	16.0
10	23.0
11	17.0
12	20.0
13	18.0
14	28.0
15	58.0
16	140.0
17	313.0
18	721.0
19	1466.0
20	2594.0
21	4310.0
22	6886.0
23	11002.0
24	19177.0
25	33418.0
26	47199.0
27	49288.0
28	44319.0
29	42007.0
30	43793.0
31	50127.0
32	60288.0
33	78714.0
34	118953.0
35	151115.0
36	180347.0
37	262816.0
38	434897.0
39	507101.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.76717593444781	4.160368219756764	53.83484774957189	22.237608096223536
2	27.861726081241134	3.7943145709339925	48.49195193285623	19.852007414968643
3	17.67618452636609	3.870081865864928	57.65561114922717	20.79812245854181
4	15.853911358772551	4.09761616567286	57.74588114110194	22.302591334452643
5	15.873201805027973	4.793745618976965	57.41952397686262	21.91352859913244
6	18.363900556122648	5.534591721191229	59.61110460349068	16.490403119195435
7	75.86839546251518	1.56187538464685	18.210506646139773	4.359222506698201
8	78.15926378360489	1.8360786194919967	16.262450472360275	3.742207124542839
9	73.25214612024968	4.374840795714639	17.827579667218885	4.545433416816807
10	32.77702475312877	27.580364463930117	26.073989784895257	13.568620998045855
11	22.418008630964	24.15122036476142	34.974927068168256	18.455843936106323
12	19.583103242468358	21.38366884764917	39.38802337537256	19.64520453450991
13	19.929866445035707	21.275549382612756	40.45043889251456	18.344145279836972
14	18.616256779545992	23.233552919039624	39.17359728241771	18.976593018996674
15	16.821687481755422	24.556273253122264	39.729115651570844	18.892923613551467
16	18.469556421998732	24.7749292993524	37.816386560090436	18.93912771855843
17	18.897804328869107	25.100775150558448	36.06346603301037	19.937954487562077
18	19.23750211497664	24.46014640286633	36.96607298575203	19.336278496405004
19	19.355708391669502	26.664788753344453	35.17415321913389	18.805349635852153
20	20.990283193047627	26.120333338911294	34.99895878073224	17.89042468730884
21	21.076695095671315	26.21636722316118	35.49827919922801	17.208658481939494
22	20.397950285498602	23.796462085673753	35.482521461202495	20.323066167625147
23	18.80623281290963	25.289821523861583	34.95103480461334	20.95291085861544
24	19.014244251447014	24.493288784023235	36.070484966467156	20.421981998062588
25	19.17554027194416	25.870022367621054	34.02341999624417	20.93101736419061
26	18.853784925004323	27.188838129449582	34.07775862678053	19.87961831876556
27	19.19250656804833	25.893496284148735	34.862066336823275	20.051930810979655
28	17.780074038127218	25.914924948543316	35.27841459491922	21.026586418410247
29	18.810090902160717	25.25230974042032	34.46338161987688	21.47421773754209
30	19.999637432576403	25.971262348209756	33.81815105488527	20.21094916432857
31	19.26418335871305	27.492232690194502	31.96422296223163	21.279360988860812
32	18.66278626557413	28.30233846691619	31.27515892538734	21.75971634212234
33	18.92267273548754	28.802030749436163	30.694400285591573	21.58089622948473
34	18.030849839447708	27.96696360008999	30.513534920820852	23.488651639641446
35	18.44162013718064	28.200866071312365	29.680327091595686	23.67718669991131
36	18.682030228826527	30.609103975040487	28.24790687037378	22.460958925759204
37	17.5888429636819	30.534080408157955	29.361035343816177	22.516041284343967
38	17.3443423677698	30.688496944207383	29.221632817743863	22.745527870278952
39	18.448220723610202	29.77617504383347	29.32431377142634	22.451290461129982
40	19.411813376320815	27.66900755069901	29.8683973218353	23.05078175114488
41	18.513947689887715	27.697036801523154	29.2872203350123	24.50179517357683
42	19.200501644568646	28.42602973796601	28.94491950073536	23.428549116729975
43	18.90249911217464	28.20802445377823	30.391610003885976	22.49786643016115
44	19.591795564034058	27.546710769739935	29.14261171260117	23.718881953624837
45	20.017998218771325	26.740091218245137	28.686613452924803	24.555297110058735
46	20.029479520518528	27.442821257978807	28.644081505156826	23.88361771634584
47	18.401877169594165	27.47791592526277	30.030530036386892	24.08967686875617
48	17.995383308139544	27.324893879304096	30.40843685098107	24.27128596157529
49	18.97473369887567	26.755012262216198	30.515673138960008	23.754580899948124
50	18.392952433013342	26.156543598267856	30.11610524495613	25.334398723762668
51	17.57703628091352	25.362381491583786	29.818706991601452	27.24187523590124
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	249.0
1	965.0
2	1681.0
3	29154.0
4	56627.0
5	38546.5
6	20466.0
7	20579.5
8	20693.0
9	21290.0
10	21887.0
11	22140.5
12	22394.0
13	22138.0
14	21882.0
15	21180.0
16	20478.0
17	19568.5
18	18659.0
19	18087.5
20	17516.0
21	17182.5
22	16849.0
23	16854.0
24	16859.0
25	18143.0
26	21880.5
27	24334.0
28	26884.0
29	29434.0
30	33942.5
31	38451.0
32	44338.5
33	50226.0
34	57208.0
35	64190.0
36	70908.5
37	77627.0
38	84332.0
39	91037.0
40	97961.5
41	104886.0
42	113714.0
43	122542.0
44	136885.5
45	151229.0
46	173601.0
47	195973.0
48	211249.5
49	226526.0
50	212132.0
51	197738.0
52	168663.5
53	139589.0
54	119158.5
55	98728.0
56	86026.0
57	73324.0
58	65425.0
59	57526.0
60	51648.0
61	45770.0
62	39160.0
63	32550.0
64	27081.5
65	21613.0
66	17505.5
67	13398.0
68	10457.5
69	7517.0
70	6218.0
71	4919.0
72	3860.0
73	2801.0
74	2310.5
75	1373.0
76	926.0
77	687.5
78	449.0
79	339.5
80	230.0
81	218.0
82	206.0
83	122.0
84	38.0
85	30.0
86	22.0
87	23.5
88	25.0
89	15.0
90	5.0
91	6.0
92	7.0
93	3.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2151324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.12680675126631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.79457897301968	28.14010686324895
2	10.257621444305512	7.616654581807519
3	3.8380658787294983	4.274853905346577
4	1.9670404656870029	2.9211972496592904
5	1.208899180353334	2.2441283125371236
6	0.8311866676172064	1.8515584069711828
7	0.6396622616652322	1.6624032022446118
8	0.5051946219189783	1.5005010479811964
9	0.4246971024045973	1.419088252391842
>10	4.200481204110266	32.979607058445296
>50	0.27150868202136763	6.454209240013112
>100	0.05651269293942302	3.72996715438693
>500	0.0021490008020573627	0.5250827019980732
>1k	0.0015169417426287263	1.3598271547812097
>5k	7.584708713143631E-4	1.5758112980235346
>10k+	1.264118118857272E-4	1.7450035701636002
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37181	1.7282845354767575	No Hit
CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT	6501	0.30218600266626505	No Hit
CGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTG	5769	0.26816044445188175	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT	5669	0.26351214414937035	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTC	5440	0.25286753645661925	No Hit
CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	5195	0.24147920071546639	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT	5002	0.2325079811316194	No Hit
TCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	4283	0.19908670195656256	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCC	4198	0.1951356466994279	No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	3472	0.16138898650319525	No Hit
CGTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCT	3059	0.14219150625382324	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTC	2542	0.11815979368983937	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGT	2442	0.113511493387328	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.394490090753415E-4	0.0	0.0	0.3312378795569612	0.0
2	1.394490090753415E-4	0.0	0.0	1.0141661600019336	0.0
3	1.394490090753415E-4	0.0	0.0	1.6528426215669978	0.0
4	1.394490090753415E-4	0.0	0.0	2.3063006780940483	0.0
5	1.394490090753415E-4	0.0	0.0	3.4890142070650447	0.0
6	1.394490090753415E-4	0.0	0.0	4.779940167078506	0.0
7	1.394490090753415E-4	0.0	0.0	5.843238861277985	0.0
8	1.394490090753415E-4	0.0	0.0	7.172420332781115	0.0
9	1.394490090753415E-4	0.0	0.0	7.9435733529677535	0.0
10	1.394490090753415E-4	0.0	0.0	8.966245902523283	0.0
11	1.394490090753415E-4	0.0	0.0	10.69206683883971	0.0
12	1.394490090753415E-4	0.0	0.0	12.07605177090945	0.0
13	1.394490090753415E-4	0.0	0.0	12.637473481446774	0.0
14	1.394490090753415E-4	0.0	0.0	12.860312997949169	0.0
15	1.394490090753415E-4	0.0	0.0	13.099653980525481	0.0
16	1.394490090753415E-4	0.0	0.0	13.613941925995341	0.0
17	1.394490090753415E-4	0.0	0.0	14.39973709213489	0.0
18	1.8593201210045536E-4	0.0	0.0	15.244798087131459	0.0
19	1.8593201210045536E-4	0.0	0.0	15.856374957932882	0.0
20	2.3241501512556918E-4	0.0	0.0	16.47445944915782	0.0
21	2.3241501512556918E-4	0.0	0.0	17.349176600084412	0.0
22	2.3241501512556918E-4	0.0	0.0	18.313605946849474	0.0
23	2.78898018150683E-4	0.0	0.0	19.306111027441705	0.0
24	3.718640242009107E-4	0.0	0.0	20.040449509232452	0.0
25	3.718640242009107E-4	0.0	0.0	20.695162606841183	0.0
26	3.718640242009107E-4	0.0	0.0	21.269181211198312	0.0
27	3.718640242009107E-4	0.0	0.0	21.83906282828621	0.0
28	3.718640242009107E-4	0.0	0.0	22.471045737415658	0.0
29	3.718640242009107E-4	0.0	0.0	23.12459675994876	0.0
30	3.718640242009107E-4	0.0	0.0	23.834671114160397	0.0
31	3.718640242009107E-4	0.0	0.0	24.51871498667797	0.0
32	3.718640242009107E-4	0.0	0.0	25.154741917070606	0.0
33	3.718640242009107E-4	0.0	0.0	25.786771309203075	0.0
34	4.183470272260245E-4	0.0	0.0	26.401137160186007	0.0
35	4.183470272260245E-4	0.0	0.0	27.05371203965558	0.0
36	4.183470272260245E-4	0.0	0.0	27.698849638641136	0.0
37	4.183470272260245E-4	0.0	0.0	28.3373401681941	0.0
38	4.183470272260245E-4	0.0	0.0	28.983221495228054	0.0
39	4.183470272260245E-4	0.0	0.0	29.634401884606874	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAT	20	7.0346595E-4	45.0	18
CGTTTTT	24075	0.0	44.27103	1
GTTTTTT	27435	0.0	39.054127	2
TGGGCGA	2655	0.0	38.81356	6
CGTTAGT	30	1.1402529E-4	37.499996	16
TTCGTTG	940	0.0	37.101063	1
TATTGCG	85	0.0	37.058823	1
TTGGGAC	4550	0.0	36.98901	5
TCTTGCG	645	0.0	36.976746	1
TTTCGCG	555	0.0	36.89189	1
CGTTCTG	855	0.0	36.842106	1
TTTGGGC	4815	0.0	36.775703	4
GGCGATT	2425	0.0	36.742264	8
TTGGGCG	2115	0.0	36.70213	5
TTGGGAT	7330	0.0	36.681446	5
GTTGGGA	3640	0.0	36.65522	4
GGGCGAT	4520	0.0	36.58739	7
GCGATTG	615	0.0	36.585365	9
GCGGGCG	480	0.0	36.5625	5
CGTTTTC	705	0.0	36.382977	1
>>END_MODULE