##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934391.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	948056
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.921000447230966	33.0	31.0	34.0	30.0	34.0
2	32.11250917667311	33.0	31.0	34.0	30.0	34.0
3	32.172922274633564	34.0	31.0	34.0	30.0	34.0
4	35.74800750166657	37.0	35.0	37.0	35.0	37.0
5	35.717190756664166	37.0	35.0	37.0	35.0	37.0
6	35.37520990321247	37.0	35.0	37.0	33.0	37.0
7	35.77821035888175	37.0	35.0	37.0	35.0	37.0
8	35.882038613752776	37.0	35.0	37.0	35.0	37.0
9	37.65327048191246	39.0	37.0	39.0	35.0	39.0
10	36.97017792197929	39.0	37.0	39.0	33.0	39.0
11	36.87757579721029	39.0	37.0	39.0	33.0	39.0
12	36.768078046022595	39.0	35.0	39.0	33.0	39.0
13	36.68549853595146	39.0	35.0	39.0	32.0	39.0
14	37.75937497363025	39.0	37.0	41.0	33.0	41.0
15	37.76784071826981	39.0	37.0	41.0	33.0	41.0
16	37.82041461685807	39.0	37.0	41.0	33.0	41.0
17	37.763614174690105	39.0	36.0	41.0	33.0	41.0
18	37.75534989494292	39.0	36.0	41.0	33.0	41.0
19	37.782277629169585	39.0	37.0	41.0	33.0	41.0
20	37.74268925042403	39.0	36.0	41.0	33.0	41.0
21	37.570423055178175	39.0	36.0	41.0	32.0	41.0
22	37.68749314386492	39.0	36.0	41.0	33.0	41.0
23	37.638205970955305	39.0	36.0	41.0	33.0	41.0
24	37.58763828297062	39.0	36.0	41.0	33.0	41.0
25	37.51043820196276	39.0	36.0	41.0	33.0	41.0
26	37.44791763355751	39.0	36.0	41.0	32.0	41.0
27	37.38502261469787	39.0	36.0	41.0	32.0	41.0
28	37.247849283164705	39.0	36.0	40.0	32.0	41.0
29	37.17058169559604	39.0	36.0	40.0	32.0	41.0
30	37.14375522121056	39.0	36.0	40.0	31.0	41.0
31	37.04476317854642	39.0	35.0	40.0	31.0	41.0
32	36.89936037533648	39.0	35.0	40.0	31.0	41.0
33	36.82233749905069	39.0	35.0	40.0	31.0	41.0
34	36.621603576160055	39.0	35.0	40.0	30.0	41.0
35	36.59129523994363	39.0	35.0	40.0	30.0	41.0
36	36.40638738639912	38.0	35.0	40.0	30.0	41.0
37	36.29626098036403	38.0	35.0	40.0	30.0	41.0
38	36.170736749727865	38.0	35.0	40.0	30.0	41.0
39	36.14923696490503	38.0	35.0	40.0	30.0	41.0
40	36.048726024623015	38.0	35.0	40.0	30.0	41.0
41	36.069545469887856	38.0	35.0	40.0	30.0	41.0
42	36.001589568548695	38.0	35.0	40.0	30.0	41.0
43	35.787283662568456	38.0	35.0	40.0	29.0	41.0
44	35.59242070088687	38.0	34.0	40.0	28.0	41.0
45	35.72990730505371	38.0	35.0	40.0	29.0	41.0
46	35.70068118339001	38.0	34.0	40.0	29.0	41.0
47	35.45054195110837	38.0	34.0	40.0	27.0	41.0
48	35.32475402296911	38.0	34.0	40.0	27.0	41.0
49	35.26288953395158	38.0	34.0	40.0	27.0	41.0
50	35.09147349945573	38.0	34.0	40.0	26.0	41.0
51	34.199923844161106	37.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	13.0
11	7.0
12	15.0
13	13.0
14	14.0
15	20.0
16	38.0
17	71.0
18	121.0
19	243.0
20	479.0
21	925.0
22	1457.0
23	2319.0
24	3486.0
25	5453.0
26	7583.0
27	9743.0
28	11761.0
29	14513.0
30	18691.0
31	24086.0
32	31485.0
33	42325.0
34	64593.0
35	81673.0
36	95109.0
37	139615.0
38	210954.0
39	181188.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.405579417249616	4.604158404144902	59.6163095850878	12.373952593517682
2	20.11210308251833	4.212303914536694	54.05682786670829	21.61876513623668
3	19.335250238382542	4.134143974617533	53.953669403495155	22.576936383504773
4	18.79087311298067	4.298480258550128	52.27855738479583	24.63208924367337
5	16.971254862581958	5.576569316580455	52.59436151450969	24.857814306327896
6	20.220219058789777	6.113879348899221	55.82708194452648	17.83881964778452
7	81.5541487000768	1.4516020150708397	11.513982296404432	5.48026698844794
8	83.36321905035146	1.6086602479178445	8.593690668061802	6.434430033668898
9	78.1122634106002	4.091319500113917	11.756056604251226	6.040360485034639
10	36.11179086467466	26.430822651826475	20.789067312479432	16.668319171019434
11	23.93097032242821	27.020344789759253	28.11068122558161	20.938003662230923
12	21.129026133477346	23.92031694330293	33.64579729467458	21.30485962854515
13	21.591235116912923	25.620427485296226	34.63687799032969	18.15145940746116
14	20.146594715923953	27.11190056283595	32.36486030361076	20.37664441762934
15	17.10848304319576	28.297484536778416	34.28162471415191	20.312407705873913
16	18.62411081201954	26.508666154741917	31.80339558000793	23.063827453230612
17	18.751423966516747	26.55961251234104	28.593669572261554	26.095293948880656
18	19.30677090804763	25.523597762157507	33.161015804973545	22.008615524821316
19	20.278127030470774	28.71866218873147	30.441028799986498	20.56218198081126
20	22.093737078822347	28.50527816922207	29.84981899803387	19.55116575392171
21	21.63131713738429	28.664129545090162	30.457589003181244	19.2469643143443
22	19.841971360341585	26.245812483650756	28.91611887905356	24.996097276954103
23	18.677483186647205	27.35302555967158	27.953939429738327	26.01555182394289
24	19.76360046241994	25.999624494755587	29.93272549300885	24.304049549815623
25	18.605124591796265	27.114537537866962	27.743508822263664	26.53682904807311
26	17.67564363286557	27.8197701401605	29.076341481937778	25.42824474503616
27	17.831647075700168	27.320116111284566	29.37938265250154	25.468854160513725
28	15.421979292362476	27.748466335321964	30.603993856902967	26.225560515412592
29	15.34761659648797	26.527863332967673	30.47562591239336	27.648894158150995
30	18.924198570548576	29.368518315373777	27.608917616680873	24.098365497396777
31	19.076721206342242	32.57064983503084	25.244394845874083	23.108234112752832
32	17.9172960247074	34.473807454411975	23.471398314023645	24.13749820685698
33	20.080881298151166	30.534799632089243	24.88070324959707	24.503615820162523
34	18.1218198081126	29.938210401073356	26.173137451796098	25.76683233901795
35	17.641362957462427	28.134519479861947	24.991667158902008	29.23245040377362
36	19.476592100044723	29.63970482756293	24.708877956576405	26.174825115815942
37	17.92415215978803	32.02648366763145	26.40118305247791	23.64818112010261
38	16.621381015467442	36.15957285223658	24.80011729264938	22.418928839646604
39	20.105457905440186	31.146472360282512	26.9393369168066	21.8087328174707
40	21.97254170639709	28.542195819656225	26.60876572691909	22.876496747027602
41	19.906102593095767	28.357607567485466	24.80001181364814	26.936278025770626
42	21.81928071759474	27.56240137713384	24.172939151273766	26.445378753997655
43	20.717447070637178	27.48413595821344	26.010805268887072	25.787611702262314
44	18.21653995122651	28.336828204241097	24.66415486005046	28.78247698448193
45	20.101027787388087	27.426860860539882	24.119250339642385	28.35286101242965
46	20.833579451002894	27.73665268718303	25.771473415072528	25.658294446741543
47	17.39538592656974	29.04606900858177	27.637291468014546	25.921253596833942
48	17.670369682803546	28.85135477229193	27.424645801513837	26.053629743390687
49	20.42780173323095	27.31663530424363	28.105196317517105	24.15036664500831
50	20.12317837764858	26.545583805176065	25.916507041778125	27.414730775397235
51	18.87198646493456	26.733230948382797	23.539115832820002	30.855666753862643
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	158.0
1	417.5
2	677.0
3	2212.0
4	3747.0
5	2608.5
6	1470.0
7	1592.5
8	1715.0
9	2014.5
10	2314.0
11	2475.0
12	2636.0
13	2877.5
14	3119.0
15	3015.5
16	2912.0
17	3054.5
18	3197.0
19	3373.0
20	3549.0
21	4338.5
22	5128.0
23	5107.5
24	5087.0
25	5741.0
26	6890.5
27	7386.0
28	8898.5
29	10411.0
30	12521.0
31	14631.0
32	16529.5
33	18428.0
34	22311.0
35	26194.0
36	28266.5
37	30339.0
38	33354.5
39	36370.0
40	41613.5
41	46857.0
42	55089.0
43	63321.0
44	71929.0
45	80537.0
46	96149.5
47	111762.0
48	126741.0
49	141720.0
50	127835.5
51	113951.0
52	91697.5
53	69444.0
54	56765.0
55	44086.0
56	34748.5
57	25411.0
58	22597.0
59	19783.0
60	17396.0
61	15009.0
62	12522.0
63	10035.0
64	8479.5
65	6924.0
66	6122.5
67	5321.0
68	4166.0
69	3011.0
70	2572.0
71	2133.0
72	1598.0
73	1063.0
74	993.0
75	663.0
76	403.0
77	354.5
78	306.0
79	213.0
80	120.0
81	73.0
82	26.0
83	28.0
84	30.0
85	19.5
86	9.0
87	8.5
88	8.0
89	4.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	948056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.280443369048214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.30193118728691	25.868656765441354
2	12.986487949732716	9.423110812462099
3	5.295306574672343	5.763482109124459
4	2.6436684757573237	3.836538576850064
5	1.5413066061441265	2.7959643519275943
6	1.0024963902409711	2.182260810828769
7	0.6908557542793663	1.7545187148519548
8	0.49623093314880284	1.4402782614460075
9	0.36904305550679795	1.205014110844941
>10	3.153358460535621	25.660395916887328
>50	0.4352437080102524	10.18227580364072
>100	0.0747296930532754	4.7618031739096764
>500	0.006130170133140706	1.5089669414387545
>1k	0.0026272157713460166	2.2151566919661
>5k	5.838257269657815E-4	1.401576958380157
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCTGC	6972	0.7353995966482993	No Hit
CTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCTGCT	6262	0.6605095057675918	No Hit
GCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCTGC	4831	0.509569054992532	No Hit
CCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCTGC	3496	0.36875458833655395	No Hit
TCCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCTG	2444	0.2577906790316184	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2389	0.25198933396339457	No Hit
TTCCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCT	1940	0.2046292624064401	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCC	1604	0.1691883179896546	No Hit
TGCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCTG	1556	0.16412532593011384	No Hit
GCCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCTG	1427	0.15051853477009797	No Hit
TTCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCTG	1229	0.12963369252449222	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7406735467103209	0.0
2	0.0	0.0	0.0	2.6165121047701825	0.0
3	0.0	0.0	0.0	3.7915481785886067	0.0
4	0.0	0.0	0.0	5.2351337895651735	0.0
5	0.0	0.0	0.0	7.25094298227109	0.0
6	0.0	0.0	0.0	8.593479710059322	0.0
7	0.0	0.0	0.0	9.639620444361936	0.0
8	0.0	0.0	0.0	11.476115334959117	0.0
9	0.0	0.0	0.0	12.21489025964711	0.0
10	0.0	0.0	0.0	13.85920240998422	0.0
11	0.0	0.0	0.0	17.245711223809565	0.0
12	0.0	0.0	0.0	19.836697410279562	0.0
13	0.0	0.0	0.0	20.69561291738041	0.0
14	0.0	0.0	0.0	20.946652940332637	0.0
15	1.0547900124043306E-4	0.0	0.0	21.337558118929685	0.0
16	1.0547900124043306E-4	0.0	0.0	22.492342224509944	0.0
17	1.0547900124043306E-4	0.0	0.0	24.266499025374028	0.0
18	1.0547900124043306E-4	0.0	0.0	26.28821504214941	0.0
19	1.0547900124043306E-4	0.0	0.0	27.56050275511151	0.0
20	1.0547900124043306E-4	0.0	0.0	28.825512416988026	0.0
21	1.0547900124043306E-4	0.0	0.0	30.705464656096265	0.0
22	1.0547900124043306E-4	0.0	0.0	32.64047693385201	0.0
23	1.0547900124043306E-4	0.0	0.0	34.87810846616656	0.0
24	1.0547900124043306E-4	0.0	0.0	36.43698262549891	0.0
25	1.0547900124043306E-4	0.0	0.0	37.74544963588649	0.0
26	1.0547900124043306E-4	0.0	0.0	38.800450606293296	0.0
27	1.0547900124043306E-4	0.0	0.0	39.863257022791906	0.0
28	2.1095800248086612E-4	0.0	0.0	41.02521370045651	0.0
29	2.1095800248086612E-4	0.0	0.0	42.228412667606136	0.0
30	2.1095800248086612E-4	0.0	0.0	43.429923970735906	0.0
31	2.1095800248086612E-4	0.0	0.0	44.58312589129756	0.0
32	2.1095800248086612E-4	0.0	0.0	45.64023644172918	0.0
33	2.1095800248086612E-4	0.0	0.0	46.664332064772545	0.0
34	2.1095800248086612E-4	0.0	0.0	47.65994835748099	0.0
35	2.1095800248086612E-4	0.0	0.0	48.702713763743915	0.0
36	2.1095800248086612E-4	0.0	0.0	49.68482874429358	0.0
37	2.1095800248086612E-4	0.0	0.0	50.63951918452074	0.0
38	3.164370037212992E-4	0.0	0.0	51.575012446522145	0.0
39	3.164370037212992E-4	0.0	0.0	52.496160564354845	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGGT	25	3.8902872E-5	45.0	22
ACACGCG	20	7.032662E-4	45.0	36
TCACCCG	40	6.8121153E-9	45.0	15
GGATGCG	25	3.8902872E-5	45.0	9
TTGTACG	25	3.8902872E-5	45.0	1
CGATTAG	25	3.8902872E-5	45.0	10
TCGATTA	20	7.032662E-4	45.0	12
TTATCGT	20	7.032662E-4	45.0	17
ATCGTAT	25	3.8902872E-5	45.0	13
CGTTACA	20	7.032662E-4	45.0	17
GTAGCCC	25	3.8902872E-5	45.0	28
TATCTCG	35	1.2116288E-7	45.0	1
CCATACG	20	7.032662E-4	45.0	16
CGAGATC	25	3.8902872E-5	45.0	44
TTCGGCC	20	7.032662E-4	45.0	1
TAGTGCA	20	7.032662E-4	45.0	25
TGTACGG	50	2.1827873E-11	45.0	2
GTGCGGT	20	7.032662E-4	45.0	19
CTCTACG	20	7.032662E-4	45.0	43
GCGTATT	20	7.032662E-4	45.0	9
>>END_MODULE