FastQCFastQC Report
Sat 14 Jan 2017
SRR2934390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871268
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365151.9513506349705119No Hit
TCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC70680.3777117975618671RNA PCR Primer, Index 43 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGCT67840.36253492284376154RNA PCR Primer, Index 24 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCC53880.28793310204631295No Hit
CCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC41360.22102659800734048RNA PCR Primer, Index 24 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC39290.20996458016703112RNA PCR Primer, Index 43 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCT39230.2096439419687613No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTT38650.2065444393854862No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTC33020.17645788844783322RNA PCR Primer, Index 43 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTG29980.16021221973549488RNA PCR Primer, Index 24 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCT26560.1419358424341142RNA PCR Primer, Index 24 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCT26500.14161520423584434RNA PCR Primer, Index 43 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTG26310.1405998499413232RNA PCR Primer, Index 24 (95% over 23bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGT25540.136484993063527No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTC23930.12788120140995304No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGC22200.11863613335983943No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCG21560.11521599257829451No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA253.891749E-545.039
GATCGCG207.0344243E-445.09
CGCGGAT253.891749E-545.04
GGCGTAA207.0344243E-445.08
GCCCTAT253.891749E-545.022
ATAAGCG207.0344243E-445.028
ACCGTCA207.0344243E-445.019
AATCGCG207.0344243E-445.01
CTTACGT253.891749E-545.029
CGTTTTT194400.044.3865741
CGAATAT2100.042.85714314
TTTCGCG6000.042.01
GCGATAT603.6379788E-1241.2500049
CTATGCG451.929402E-840.0000041
GCTTACG451.929402E-840.0000041
CGCCGGT850.039.70588328
GTTTTTT216300.039.694872
TCTCGCG1250.039.61
CTTACGA403.4597724E-739.37529
CGTTTGG8300.039.3072282
Produced by FastQC (version 0.11.5)