##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934390.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1871268
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95427378654474	33.0	31.0	34.0	30.0	34.0
2	32.19426613397974	33.0	31.0	34.0	30.0	34.0
3	32.157582452112685	33.0	31.0	34.0	30.0	34.0
4	35.7952377745999	37.0	35.0	37.0	35.0	37.0
5	35.803108907970426	37.0	35.0	37.0	35.0	37.0
6	35.542124912091694	37.0	35.0	37.0	33.0	37.0
7	35.87725168174735	37.0	35.0	37.0	35.0	37.0
8	35.97226265826167	37.0	35.0	37.0	35.0	37.0
9	37.755366414645046	39.0	38.0	39.0	35.0	39.0
10	37.15961102311374	39.0	37.0	39.0	33.0	39.0
11	37.01350848729311	39.0	37.0	39.0	33.0	39.0
12	36.97592808726489	39.0	37.0	39.0	33.0	39.0
13	36.96477254995009	39.0	37.0	39.0	33.0	39.0
14	38.07433943187186	40.0	37.0	41.0	33.0	41.0
15	38.08451488509396	40.0	37.0	41.0	33.0	41.0
16	38.10400968754876	40.0	37.0	41.0	33.0	41.0
17	38.04673248300083	40.0	37.0	41.0	33.0	41.0
18	37.88646682356563	39.0	37.0	41.0	33.0	41.0
19	37.78106235985439	39.0	37.0	41.0	33.0	41.0
20	37.586293892697356	39.0	35.0	41.0	33.0	41.0
21	37.42022949144644	39.0	35.0	41.0	33.0	41.0
22	37.53427087942507	39.0	35.0	41.0	33.0	41.0
23	37.48307350951333	39.0	35.0	41.0	33.0	41.0
24	37.392997154870386	39.0	35.0	41.0	33.0	41.0
25	37.3194213763074	39.0	35.0	41.0	33.0	41.0
26	37.25927873506093	39.0	35.0	41.0	33.0	41.0
27	37.1817826201271	39.0	35.0	40.0	32.0	41.0
28	37.04180641148141	39.0	35.0	40.0	32.0	41.0
29	36.94616644970149	39.0	35.0	40.0	32.0	41.0
30	36.852188996979585	39.0	35.0	40.0	31.0	41.0
31	36.659808750002675	39.0	35.0	40.0	31.0	41.0
32	36.34846745629167	39.0	35.0	40.0	30.0	41.0
33	36.0216649886601	39.0	35.0	40.0	29.0	41.0
34	35.650532686926724	39.0	35.0	40.0	26.0	41.0
35	35.45786707195335	39.0	35.0	40.0	25.0	41.0
36	35.243516161233984	38.0	35.0	40.0	24.0	41.0
37	35.172103087318334	38.0	35.0	40.0	24.0	41.0
38	35.09640414948581	38.0	35.0	40.0	24.0	41.0
39	34.98985180102476	38.0	34.0	40.0	23.0	41.0
40	34.857928955125615	38.0	34.0	40.0	23.0	41.0
41	34.77788430091254	38.0	34.0	40.0	22.0	41.0
42	34.71945974601179	38.0	34.0	40.0	22.0	41.0
43	34.66013473217091	38.0	34.0	40.0	22.0	41.0
44	34.517823208647826	38.0	34.0	40.0	21.0	41.0
45	34.56849152553242	38.0	34.0	40.0	22.0	41.0
46	34.45104068471218	38.0	34.0	40.0	21.0	41.0
47	34.22790001218425	38.0	33.0	40.0	20.0	41.0
48	34.13449596744026	38.0	33.0	40.0	20.0	41.0
49	34.14819309687335	38.0	33.0	40.0	20.0	41.0
50	34.028945078951814	38.0	33.0	40.0	20.0	41.0
51	33.22822011598552	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	14.0
10	22.0
11	19.0
12	17.0
13	16.0
14	24.0
15	36.0
16	97.0
17	284.0
18	543.0
19	1092.0
20	2020.0
21	3558.0
22	5496.0
23	8984.0
24	15229.0
25	26191.0
26	36790.0
27	38035.0
28	35458.0
29	35232.0
30	38178.0
31	45323.0
32	57194.0
33	73849.0
34	106629.0
35	138331.0
36	169630.0
37	239963.0
38	392172.0
39	400729.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.109502219885126	4.07338767082	57.45970112244745	19.35740898684742
2	26.542323173377625	3.7930964458324516	50.7460716476742	18.918508733115726
3	16.63027423116304	3.5506939679404557	59.32934245655886	20.489689344337634
4	15.464700940752474	3.9178781446591295	58.512623525865884	22.104797388722513
5	15.730723765917014	4.506783635481396	58.46880297210234	21.29368962649925
6	18.316350196764976	4.716106939251887	60.379218797093735	16.588324066889403
7	75.82478832534945	1.0938037736978348	18.469935893736224	4.611472007216498
8	78.41126979139278	1.22077650021269	15.792019101486265	4.575934606908256
9	73.67693991453923	3.9080452399121874	17.744598849550144	4.670415995998435
10	33.359411906792616	30.63056708071746	23.472746821941058	12.53727419054887
11	19.173790178638228	23.93884788282598	37.789669892286945	19.097692046248856
12	18.23469433560559	20.832130940089822	40.53898212335165	20.394192600952938
13	19.722402135877918	21.841125910345287	41.22728545563757	17.20918649813923
14	17.213515113815873	24.673590314161306	39.77645104816627	18.336443523856552
15	14.796384056158713	26.039402159391383	40.53187464329	18.6323391411599
16	17.307088028010952	26.647652821509265	37.76327068062939	18.28198846985039
17	18.00388827255102	26.69083209887627	34.97430619237865	20.330973436194068
18	17.937836803707434	25.789090605942068	37.19018334092177	19.08288924942873
19	18.38566148729097	27.84710688153701	35.39642638040088	18.370805250771134
20	18.470470290733342	28.55571729971335	34.76717391629633	18.20663849325698
21	19.09266871447596	27.916097533864736	35.780871580126416	17.210362171532886
22	18.023981599642592	25.406088278108747	35.81678305833264	20.753147063916018
23	16.92563545146927	26.172039494075676	34.87416019511903	22.028164859336023
24	17.645254447786208	26.58678500353771	35.31343452674871	20.45452602192738
25	17.595715846153517	27.33745246538711	33.130689992026795	21.93614169643258
26	16.88186833740544	28.517828552617797	33.05785168131983	21.542451428656932
27	17.210629370031445	26.512824459136798	34.85363935042976	21.422906820401995
28	16.030894559197293	26.073176049609142	34.8230718421947	23.07285754899886
29	16.89464042563652	24.202572800902917	34.018216524837705	24.884570248622857
30	18.77229771470468	25.720527471212034	32.54931949886387	22.957855315219415
31	17.74614860084178	28.722609481912798	31.135572242992453	22.395669674252964
32	17.426418877467043	28.80597541346296	30.276315311329004	23.491290397740997
33	18.2558564566914	27.162758087029758	30.6693108630084	23.912074593270447
34	16.430195995442663	27.97162138186513	30.810658868745683	24.787523753946523
35	16.334806131457384	26.854517899093022	30.794146001534788	26.016529967914803
36	16.590675413676713	27.838289331084592	30.82134680868801	24.74968844655068
37	16.414965681024846	29.957921580447056	32.149911183219075	21.47720155530902
38	18.09943845563543	29.029139599458766	32.01160924036536	20.859812704540452
39	18.261307306061987	26.80091788028225	31.970460671587393	22.967314142068375
40	18.365461280799973	27.335528636197488	31.83167777143627	22.467332311566274
41	16.911848008943668	26.84671570293512	32.54114322480799	23.700293063313218
42	17.47606435849916	26.7121010993615	32.92109948975775	22.890735052381594
43	17.55371224218017	26.109140967515078	33.374962859408704	22.962183930896053
44	18.823279188229584	26.450193131074755	29.13366765209473	25.592860028600928
45	19.696857959415752	25.36937520440685	28.03355799383092	26.900208842346473
46	19.168499648366776	25.792457307023902	29.759179337219464	25.279863707389854
47	16.497797215577886	27.34675097313693	31.58809962015062	24.567352191134567
48	16.646199261677108	26.712261418460635	32.32519339827326	24.316345921588997
49	18.946297376965777	24.90423605811674	32.12420668765778	24.025259877259696
50	18.063366658330075	24.315223687895053	31.323412787478865	26.297996866296007
51	16.69381403412018	24.044391289756465	30.6142145326057	28.647580143517658
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	318.0
1	763.0
2	1208.0
3	25128.0
4	49048.0
5	31770.5
6	14493.0
7	14921.5
8	15350.0
9	16570.5
10	17791.0
11	18625.5
12	19460.0
13	20235.0
14	21010.0
15	20339.0
16	19668.0
17	19039.5
18	18411.0
19	17331.5
20	16252.0
21	15784.0
22	15316.0
23	15638.0
24	15960.0
25	16997.5
26	19072.0
27	20109.0
28	22625.0
29	25141.0
30	28795.5
31	32450.0
32	36897.5
33	41345.0
34	48256.5
35	55168.0
36	59757.5
37	64347.0
38	70854.5
39	77362.0
40	85831.5
41	94301.0
42	104483.0
43	114665.0
44	130060.0
45	145455.0
46	172679.0
47	199903.0
48	216429.0
49	232955.0
50	210472.5
51	187990.0
52	154502.5
53	121015.0
54	98740.5
55	76466.0
56	63755.0
57	51044.0
58	42769.5
59	34495.0
60	28122.5
61	21750.0
62	17862.5
63	13975.0
64	11425.5
65	8876.0
66	6666.5
67	4457.0
68	3281.0
69	2105.0
70	1795.5
71	1486.0
72	1166.0
73	846.0
74	834.0
75	520.5
76	219.0
77	154.5
78	90.0
79	82.0
80	74.0
81	46.0
82	18.0
83	14.5
84	11.0
85	7.0
86	3.0
87	2.5
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1871268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.011786141096046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32123240857821	28.25082792057571
2	11.331316358764523	8.614471482529202
3	4.520401543020621	5.154856101755413
4	2.2799633557788814	3.4666191795761003
5	1.3374766849162314	2.5419938857868947
6	0.9160809522058259	2.089312394590769
7	0.6567130347564282	1.7473984803262061
8	0.5156783261818911	1.5681483401939533
9	0.40479200484879385	1.384818040794408
>10	3.3754698973856536	26.26689551743336
>50	0.2554509254818841	6.395027833285054
>100	0.07762010950822924	5.571550381786141
>500	0.0038312502085323438	1.0411656685481956
>1k	0.003405555740917639	2.8996002698965913
>5k	4.256944676147049E-4	1.0377675730646678
>10k+	1.418981558715683E-4	1.9695469298573984
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36515	1.9513506349705119	No Hit
TCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC	7068	0.3777117975618671	RNA PCR Primer, Index 43 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGCT	6784	0.36253492284376154	RNA PCR Primer, Index 24 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCC	5388	0.28793310204631295	No Hit
CCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC	4136	0.22102659800734048	RNA PCR Primer, Index 24 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC	3929	0.20996458016703112	RNA PCR Primer, Index 43 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCT	3923	0.2096439419687613	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTT	3865	0.2065444393854862	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTC	3302	0.17645788844783322	RNA PCR Primer, Index 43 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTG	2998	0.16021221973549488	RNA PCR Primer, Index 24 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCT	2656	0.1419358424341142	RNA PCR Primer, Index 24 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCT	2650	0.14161520423584434	RNA PCR Primer, Index 43 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTG	2631	0.1405998499413232	RNA PCR Primer, Index 24 (95% over 23bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGT	2554	0.136484993063527	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTC	2393	0.12788120140995304	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGC	2220	0.11863613335983943	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCG	2156	0.11521599257829451	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.206381982698363E-4	0.0	0.0	0.3979120040528668	0.0
2	3.206381982698363E-4	0.0	0.0	1.3105551957282442	0.0
3	3.206381982698363E-4	0.0	0.0	2.0654978335545735	0.0
4	3.206381982698363E-4	0.0	0.0	3.128092822620811	0.0
5	3.206381982698363E-4	0.0	0.0	4.811870881135145	0.0
6	3.206381982698363E-4	0.0	0.0	6.594939901713705	0.0
7	3.206381982698363E-4	0.0	0.0	8.042461047802881	0.0
8	3.206381982698363E-4	0.0	0.0	9.894146642811185	0.0
9	3.206381982698363E-4	0.0	0.0	10.942045714456722	0.0
10	3.206381982698363E-4	0.0	0.0	12.317530145334608	0.0
11	3.206381982698363E-4	0.0	0.0	14.97139907271433	0.0
12	3.206381982698363E-4	0.0	0.0	17.0309116599012	0.0
13	3.206381982698363E-4	0.0	0.0	17.792267061692925	0.0
14	3.206381982698363E-4	0.0	0.0	18.054976625475348	0.0
15	3.206381982698363E-4	0.0	0.0	18.34258908932339	0.0
16	3.206381982698363E-4	0.0	0.0	18.977666480696513	0.0
17	3.206381982698363E-4	0.0	0.0	20.08007404604792	0.0
18	3.206381982698363E-4	0.0	0.0	21.271886229016904	0.0
19	3.206381982698363E-4	0.0	0.0	22.158290528133865	0.0
20	3.7407789798147564E-4	0.0	0.0	23.02684596754714	0.0
21	3.7407789798147564E-4	0.0	0.0	24.25873792529985	0.0
22	3.7407789798147564E-4	0.0	0.0	25.600982862957096	0.0
23	3.7407789798147564E-4	0.0	0.0	26.918164581449584	0.0
24	3.7407789798147564E-4	0.0	0.0	27.877834708871205	0.0
25	3.7407789798147564E-4	0.0	0.0	28.676277262262808	0.0
26	3.7407789798147564E-4	0.0	0.0	29.38071938386164	0.0
27	4.2751759769311503E-4	0.0	0.0	30.103918840059254	0.0
28	4.809572974047544E-4	0.0	0.0	30.869335659029066	0.0
29	4.809572974047544E-4	0.0	0.0	31.68183285344483	0.0
30	4.809572974047544E-4	0.0	0.0	32.60115600758416	0.0
31	4.809572974047544E-4	0.0	0.0	33.3979419302847	0.0
32	4.809572974047544E-4	0.0	0.0	34.134340992311095	0.0
33	4.809572974047544E-4	0.0	0.0	34.88329838376972	0.0
34	4.809572974047544E-4	0.0	0.0	35.63995109198682	0.0
35	5.343969971163938E-4	0.0	0.0	36.43508038399631	0.0
36	5.343969971163938E-4	0.0	0.0	37.19344316260418	0.0
37	5.343969971163938E-4	0.0	0.0	37.897938723902726	0.0
38	5.343969971163938E-4	0.0	0.0	38.67350908581774	0.0
39	5.343969971163938E-4	0.0	0.0	39.54954608319065	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	25	3.891749E-5	45.0	39
GATCGCG	20	7.0344243E-4	45.0	9
CGCGGAT	25	3.891749E-5	45.0	4
GGCGTAA	20	7.0344243E-4	45.0	8
GCCCTAT	25	3.891749E-5	45.0	22
ATAAGCG	20	7.0344243E-4	45.0	28
ACCGTCA	20	7.0344243E-4	45.0	19
AATCGCG	20	7.0344243E-4	45.0	1
CTTACGT	25	3.891749E-5	45.0	29
CGTTTTT	19440	0.0	44.386574	1
CGAATAT	210	0.0	42.857143	14
TTTCGCG	600	0.0	42.0	1
GCGATAT	60	3.6379788E-12	41.250004	9
CTATGCG	45	1.929402E-8	40.000004	1
GCTTACG	45	1.929402E-8	40.000004	1
CGCCGGT	85	0.0	39.705883	28
GTTTTTT	21630	0.0	39.69487	2
TCTCGCG	125	0.0	39.6	1
CTTACGA	40	3.4597724E-7	39.375	29
CGTTTGG	830	0.0	39.307228	2
>>END_MODULE