SRR2934389.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934389.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1490936
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGCT	7621	0.5111554084145798	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC	6601	0.442742009046666	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC	5941	0.39847451533801587	TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC	4977	0.3338171457393208	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3430	0.23005682336465147	No Hit
TCCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTG	2719	0.18236865968760563	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCC	2332	0.15641181110389715	No Hit
TTCCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCT	1955	0.13112568212183487	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGGA	35	1.2122473E-7	45.000004	3
TTAGGCG	40	6.8175723E-9	45.0	13
CGACGTG	20	7.033963E-4	45.0	24
TAGCGAA	20	7.033963E-4	45.0	33
AAGCGTA	25	3.8913662E-5	45.0	18
TACGGAC	25	3.8913662E-5	45.0	32
CGCTAGA	20	7.033963E-4	45.0	12
AGCGATG	20	7.033963E-4	45.0	35
GTCCGCG	50	2.1827873E-11	45.0	1
CGGTGCG	20	7.033963E-4	45.0	26
CCGTTCG	40	6.8175723E-9	45.0	1
GCGTAAA	25	3.8913662E-5	45.0	29
GTCGGTA	20	7.033963E-4	45.0	32
GTACGGC	90	0.0	45.0	9
CCCGAAC	20	7.033963E-4	45.0	9
TCGTCCG	30	2.165938E-6	44.999996	1
GTCCGGT	30	2.165938E-6	44.999996	3
GTTACCG	105	0.0	42.857147	1
CTTCCGT	90	0.0	42.5	19
CGTTTTT	2680	0.0	42.481342	1