##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934389.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1490936
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.128393170464726	33.0	31.0	34.0	30.0	34.0
2	32.31091542494111	34.0	31.0	34.0	30.0	34.0
3	32.377181180144554	34.0	31.0	34.0	30.0	34.0
4	35.909593034174506	37.0	35.0	37.0	35.0	37.0
5	35.89057477986983	37.0	35.0	37.0	35.0	37.0
6	35.575115900347164	37.0	35.0	37.0	33.0	37.0
7	35.93287505298685	37.0	35.0	37.0	35.0	37.0
8	36.02726676396572	37.0	35.0	37.0	35.0	37.0
9	37.83400159362977	39.0	38.0	39.0	35.0	39.0
10	37.116854781157606	39.0	37.0	39.0	33.0	39.0
11	37.0094551342244	39.0	37.0	39.0	33.0	39.0
12	36.97382114322814	39.0	37.0	39.0	33.0	39.0
13	36.99375560050867	39.0	37.0	39.0	33.0	39.0
14	38.15792830812322	40.0	37.0	41.0	33.0	41.0
15	38.17880110212645	40.0	37.0	41.0	33.0	41.0
16	38.19541683881803	40.0	37.0	41.0	33.0	41.0
17	38.14707673568819	40.0	37.0	41.0	33.0	41.0
18	38.11022069357772	40.0	37.0	41.0	33.0	41.0
19	38.13971692949932	40.0	37.0	41.0	34.0	41.0
20	38.09813566779526	40.0	37.0	41.0	34.0	41.0
21	37.96892287797733	40.0	37.0	41.0	33.0	41.0
22	38.05262197706676	40.0	37.0	41.0	34.0	41.0
23	37.98677072657713	40.0	37.0	41.0	33.0	41.0
24	37.94011010532981	40.0	37.0	41.0	33.0	41.0
25	37.887949583348984	40.0	37.0	41.0	33.0	41.0
26	37.84300130924466	40.0	37.0	41.0	33.0	41.0
27	37.777856997215174	40.0	37.0	41.0	33.0	41.0
28	37.71945542934103	40.0	37.0	41.0	33.0	41.0
29	37.66637065574914	40.0	36.0	41.0	33.0	41.0
30	37.60838828762603	39.0	36.0	41.0	33.0	41.0
31	37.531765951053565	39.0	36.0	41.0	33.0	41.0
32	37.42934170212538	39.0	36.0	41.0	32.0	41.0
33	37.37908669453283	39.0	36.0	41.0	32.0	41.0
34	37.25786821164691	39.0	36.0	41.0	32.0	41.0
35	37.20547025492711	39.0	36.0	41.0	32.0	41.0
36	37.04414609346075	39.0	36.0	41.0	31.0	41.0
37	36.992677754108826	39.0	35.0	41.0	31.0	41.0
38	36.989819817886215	39.0	35.0	41.0	31.0	41.0
39	36.90382551631995	39.0	35.0	41.0	31.0	41.0
40	36.73791832781555	39.0	35.0	41.0	30.0	41.0
41	36.689385728160026	39.0	35.0	40.0	30.0	41.0
42	36.673021511319064	39.0	35.0	40.0	30.0	41.0
43	36.61867645559568	39.0	35.0	40.0	30.0	41.0
44	36.4865138409697	39.0	35.0	40.0	30.0	41.0
45	36.54012043441167	39.0	35.0	40.0	30.0	41.0
46	36.40777337189524	39.0	35.0	40.0	30.0	41.0
47	36.181301544801386	39.0	35.0	40.0	29.0	41.0
48	36.10623863130275	38.0	35.0	40.0	29.0	41.0
49	36.117974212172754	39.0	35.0	40.0	29.0	41.0
50	35.96954530576765	38.0	35.0	40.0	28.0	41.0
51	34.960200169557915	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	13.0
11	15.0
12	8.0
13	11.0
14	12.0
15	13.0
16	32.0
17	65.0
18	152.0
19	303.0
20	616.0
21	1130.0
22	1795.0
23	2850.0
24	4759.0
25	6979.0
26	10047.0
27	12410.0
28	14381.0
29	18080.0
30	22673.0
31	30386.0
32	40402.0
33	54806.0
34	83938.0
35	108716.0
36	134026.0
37	203751.0
38	351147.0
39	387328.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.334828590898603	4.25645366400704	59.24218075088401	14.166536994210349
2	18.740375173716377	4.15805909844554	56.25244812654601	20.849117601292075
3	18.58047562068392	4.3248670633749535	55.660538078093225	21.434119237847902
4	17.6016945060016	4.521320834697129	54.254307361281775	23.622677298019497
5	17.54220167733558	5.282319294724925	54.45143185220559	22.7240471757339
6	20.496386162786333	5.709634752933727	56.387933486078545	17.4060455982014
7	84.47136563876651	1.5206554808522967	9.541589981058879	4.466388899322305
8	86.925931092951	1.565996125923581	7.130218869220409	4.377853911905005
9	82.19205921649218	4.156784731202412	8.932040007082799	4.719116045222598
10	35.44075667902579	28.901173491015044	20.02614465007217	15.631925179886997
11	22.63450610891413	27.524186148835362	28.632885650356556	21.20842209189395
12	19.141532567460978	24.127393798258275	35.32096615817178	21.410107476108966
13	21.176965342576747	24.814009454463502	35.62540578535899	18.383619417600755
14	19.986438049654716	26.554862180536254	33.47507874248124	19.9836210273278
15	17.567823166118465	27.856460639490898	34.0435136048764	20.532202589514238
16	19.07251552045158	27.038182725482518	31.850059291612787	22.039242462453117
17	19.761948198983724	26.795315157726424	30.180034555473878	23.262702087815974
18	20.522678371170862	25.61900712035929	32.86660191986779	20.99171258860206
19	21.16516067758777	28.077798108034145	31.042915322991732	19.71412589138635
20	21.853922636518266	27.80568716564628	31.071756265862522	19.268633931972936
21	22.105844918896587	27.978665750910835	31.2439299876051	18.671559342587475
22	20.94469514452666	25.477418212451774	30.158437384300868	23.419449258720697
23	18.839574602799853	27.802333567638048	29.446736814994072	23.91135501456803
24	19.666303583788974	27.141406472175866	30.513315125531882	22.678974818503274
25	18.80047165002388	28.22012480750347	29.425877435382873	23.553526107089777
26	17.84395842611621	29.34908004099438	31.100865496573967	21.706096036315444
27	18.33002892142922	28.94611170432534	31.152645049821054	21.57121432442439
28	17.353327037512006	26.85044830898174	32.129145717857774	23.667078935648476
29	19.112155048908875	25.012676600471114	32.07542107776592	23.7997472728541
30	20.97769454892765	27.26904441236914	30.45160892218043	21.301652116522778
31	19.72821100302092	28.36164664345083	29.82683361324698	22.083308740281275
32	19.703595593640504	28.523692499208554	29.651105077615675	22.121606829535274
33	21.485563431294167	26.854137266790794	29.058859669362064	22.60143963255297
34	19.10450884545011	26.168259402147378	30.542223140362836	24.185008612039685
35	19.202635123170946	25.504381140437953	31.475059962332384	23.81792377405871
36	20.37330911588425	27.66651284830469	30.006787682368657	21.9533903534424
37	18.449886514243403	28.195174038322236	30.818760832121566	22.536178615312796
38	21.15442916396143	27.187417836848798	28.74811527791937	22.910037721270395
39	23.54675183911315	24.704548015474842	30.170845696931327	21.577854448480686
40	21.743253902246643	24.71668804026464	31.933161450256755	21.606896607231967
41	19.144416661748057	25.314969924933063	32.30393524604678	23.236678167272103
42	21.021693754795646	25.331134267332732	30.572204306556415	23.0749676713152
43	21.469264944974164	25.6461041922658	29.321379321446393	23.563251541313647
44	20.243726088846202	24.975787022380572	28.544753094700244	26.235733794072985
45	21.892690229493418	24.026115138409697	28.562393020223535	25.51880161187335
46	21.730711445695857	24.805021811801446	30.813059715507574	22.651207026995124
47	18.615285968009356	25.825722901586655	32.708110877998784	22.8508802524052
48	19.859739116903743	24.91844049643982	31.603033262326484	23.618787124329952
49	22.663011691984096	22.576623007292064	31.796133435640428	22.964231865083413
50	22.169697424973307	21.823136606802706	30.594673413211567	25.412492555012424
51	20.652194326248747	22.0189867304834	29.560088427672284	27.768730515595575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	218.0
1	558.0
2	898.0
3	3276.5
4	5655.0
5	3927.5
6	2200.0
7	2422.0
8	2644.0
9	3092.0
10	3540.0
11	3877.5
12	4215.0
13	4333.5
14	4452.0
15	4466.0
16	4480.0
17	4961.5
18	5443.0
19	5308.0
20	5173.0
21	5529.0
22	5885.0
23	7065.5
24	8246.0
25	9583.5
26	13149.5
27	15378.0
28	18914.5
29	22451.0
30	25611.5
31	28772.0
32	32751.0
33	36730.0
34	41564.5
35	46399.0
36	52052.0
37	57705.0
38	64894.5
39	72084.0
40	80138.0
41	88192.0
42	98399.0
43	108606.0
44	119307.5
45	130009.0
46	148444.0
47	166879.0
48	180810.0
49	194741.0
50	178258.5
51	161776.0
52	133791.0
53	105806.0
54	86804.0
55	67802.0
56	55519.5
57	43237.0
58	36281.0
59	29325.0
60	25077.5
61	20830.0
62	16690.5
63	12551.0
64	10090.5
65	7630.0
66	6138.0
67	4646.0
68	3542.0
69	2438.0
70	1895.5
71	1353.0
72	1041.5
73	730.0
74	564.0
75	317.0
76	236.0
77	173.5
78	111.0
79	83.5
80	56.0
81	56.0
82	56.0
83	38.5
84	21.0
85	18.5
86	16.0
87	8.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1490936.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.29097140947661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.64215787751712	22.165219575076847
2	13.336068508586452	8.61269213851173
3	5.610294619006851	5.4348558942327205
4	2.9064704847700487	3.7541102130478907
5	1.6636006864241275	2.685964110105358
6	1.0578121644801441	2.049466941589494
7	0.7161841234732528	1.618839673849711
8	0.5606396610592592	1.4482879413026557
9	0.4601634822487132	1.3373213264080557
>10	4.527124907165653	33.45340415952022
>50	0.43235731717922954	8.795364998349124
>100	0.08084303275825207	4.970634914836959
>500	0.003351005509839295	0.7815942984352257
>1k	0.002303816288014515	1.5286299149893057
>5k	6.283135330948679E-4	1.3636138997446965
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGCT	7621	0.5111554084145798	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC	6601	0.442742009046666	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC	5941	0.39847451533801587	TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC	4977	0.3338171457393208	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3430	0.23005682336465147	No Hit
TCCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTG	2719	0.18236865968760563	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCC	2332	0.15641181110389715	No Hit
TTCCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCT	1955	0.13112568212183487	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0121588049386427E-4	0.0	0.0	0.5540814629199375	0.0
2	2.0121588049386427E-4	0.0	0.0	1.870167465270139	0.0
3	2.0121588049386427E-4	0.0	0.0	2.6682567192689692	0.0
4	2.0121588049386427E-4	0.0	0.0	3.5896242360503736	0.0
5	2.0121588049386427E-4	0.0	0.0	5.01302537466397	0.0
6	2.0121588049386427E-4	0.0	0.0	5.871680608691453	0.0
7	2.0121588049386427E-4	0.0	0.0	6.662794378833163	0.0
8	2.0121588049386427E-4	0.0	0.0	7.892223408650673	0.0
9	2.0121588049386427E-4	0.0	0.0	8.430207601131102	0.0
10	2.0121588049386427E-4	0.0	0.0	9.65205749945001	0.0
11	2.0121588049386427E-4	0.0	0.0	12.072617469831032	0.0
12	2.0121588049386427E-4	0.0	0.0	14.070557019214775	0.0
13	2.0121588049386427E-4	0.0	0.0	14.70733820901769	0.0
14	2.0121588049386427E-4	0.0	0.0	14.906474858746451	0.0
15	2.0121588049386427E-4	0.0	0.0	15.199378108785353	0.0
16	2.0121588049386427E-4	0.0	0.0	16.02771681681843	0.0
17	2.0121588049386427E-4	0.0	0.0	17.431801230904615	0.0
18	2.0121588049386427E-4	0.0	0.0	18.917243932670484	0.0
19	2.0121588049386427E-4	0.0	0.0	19.979797925598415	0.0
20	2.0121588049386427E-4	0.0	0.0	20.978968916170782	0.0
21	2.0121588049386427E-4	0.0	0.0	22.483862486384393	0.0
22	2.0121588049386427E-4	0.0	0.0	24.08969935664576	0.0
23	2.0121588049386427E-4	0.0	0.0	25.77166290169397	0.0
24	2.0121588049386427E-4	0.0	0.0	26.92737984729056	0.0
25	2.6828784065848567E-4	0.0	0.0	27.926953269623915	0.0
26	2.6828784065848567E-4	0.0	0.0	28.805797163660948	0.0
27	2.6828784065848567E-4	0.0	0.0	29.700000536575683	0.0
28	2.6828784065848567E-4	0.0	0.0	30.64175792924713	0.0
29	2.6828784065848567E-4	0.0	0.0	31.619600036487146	0.0
30	2.6828784065848567E-4	0.0	0.0	32.66894085326265	0.0
31	2.6828784065848567E-4	0.0	0.0	33.66891670735699	0.0
32	2.6828784065848567E-4	0.0	0.0	34.587936705532634	0.0
33	2.6828784065848567E-4	0.0	0.0	35.48958506602564	0.0
34	2.6828784065848567E-4	0.0	0.0	36.41021479124523	0.0
35	2.6828784065848567E-4	0.0	0.0	37.32514339985083	0.0
36	2.6828784065848567E-4	0.0	0.0	38.22638932858285	0.0
37	2.6828784065848567E-4	0.0	0.0	39.10751366926548	0.0
38	2.6828784065848567E-4	0.0	0.0	40.000308531016756	0.0
39	2.6828784065848567E-4	0.0	0.0	40.892298529246055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGGA	35	1.2122473E-7	45.000004	3
TTAGGCG	40	6.8175723E-9	45.0	13
CGACGTG	20	7.033963E-4	45.0	24
TAGCGAA	20	7.033963E-4	45.0	33
AAGCGTA	25	3.8913662E-5	45.0	18
TACGGAC	25	3.8913662E-5	45.0	32
CGCTAGA	20	7.033963E-4	45.0	12
AGCGATG	20	7.033963E-4	45.0	35
GTCCGCG	50	2.1827873E-11	45.0	1
CGGTGCG	20	7.033963E-4	45.0	26
CCGTTCG	40	6.8175723E-9	45.0	1
GCGTAAA	25	3.8913662E-5	45.0	29
GTCGGTA	20	7.033963E-4	45.0	32
GTACGGC	90	0.0	45.0	9
CCCGAAC	20	7.033963E-4	45.0	9
TCGTCCG	30	2.165938E-6	44.999996	1
GTCCGGT	30	2.165938E-6	44.999996	3
GTTACCG	105	0.0	42.857147	1
CTTCCGT	90	0.0	42.5	19
CGTTTTT	2680	0.0	42.481342	1
>>END_MODULE