##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934388.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469692
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07941800158402	33.0	31.0	34.0	30.0	34.0
2	32.25891222332933	34.0	31.0	34.0	30.0	34.0
3	32.34140245096787	34.0	31.0	34.0	30.0	34.0
4	35.87094521516228	37.0	35.0	37.0	35.0	37.0
5	35.83209422344856	37.0	35.0	37.0	35.0	37.0
6	35.42359673999131	37.0	35.0	37.0	33.0	37.0
7	35.81822343152534	37.0	35.0	37.0	35.0	37.0
8	35.87482861108982	37.0	35.0	37.0	35.0	37.0
9	37.665789496095314	39.0	38.0	39.0	35.0	39.0
10	37.1736968055662	39.0	37.0	39.0	34.0	39.0
11	37.00753898299311	39.0	37.0	39.0	33.0	39.0
12	36.69547277790552	39.0	35.0	39.0	33.0	39.0
13	36.46940761179667	39.0	35.0	39.0	32.0	39.0
14	37.51910826669392	39.0	36.0	41.0	33.0	41.0
15	37.63058344617324	39.0	36.0	41.0	33.0	41.0
16	37.74666802926173	39.0	36.0	41.0	33.0	41.0
17	37.679128024322324	39.0	36.0	41.0	33.0	41.0
18	37.679915774592715	39.0	36.0	41.0	33.0	41.0
19	37.73761741737138	39.0	36.0	41.0	33.0	41.0
20	37.630370540694756	39.0	35.0	41.0	33.0	41.0
21	37.47688485220102	39.0	35.0	41.0	33.0	41.0
22	37.55090569990547	39.0	35.0	41.0	33.0	41.0
23	37.55944108053789	39.0	35.0	41.0	33.0	41.0
24	37.489452662595916	39.0	35.0	41.0	33.0	41.0
25	37.41309837084728	39.0	35.0	41.0	33.0	41.0
26	37.35163043015423	39.0	35.0	41.0	33.0	41.0
27	37.218351600623386	39.0	35.0	41.0	32.0	41.0
28	37.10545847065737	39.0	35.0	41.0	32.0	41.0
29	37.067361164337484	39.0	35.0	41.0	32.0	41.0
30	37.04184870085077	39.0	35.0	41.0	32.0	41.0
31	37.010415336007426	39.0	35.0	41.0	32.0	41.0
32	36.89456707800005	39.0	35.0	41.0	31.0	41.0
33	36.823712134760655	39.0	35.0	41.0	31.0	41.0
34	36.72844757841309	39.0	35.0	41.0	31.0	41.0
35	36.683978011122186	39.0	35.0	41.0	31.0	41.0
36	36.480670311608456	39.0	35.0	40.0	30.0	41.0
37	36.41802500361939	38.0	35.0	40.0	30.0	41.0
38	36.38758590736057	38.0	35.0	40.0	30.0	41.0
39	36.373712986382564	38.0	35.0	40.0	30.0	41.0
40	36.31479565332175	38.0	35.0	40.0	30.0	41.0
41	36.23144315849535	38.0	35.0	40.0	30.0	41.0
42	36.14134368905581	38.0	35.0	40.0	30.0	41.0
43	36.03195498326563	38.0	35.0	40.0	30.0	41.0
44	35.91768009674425	38.0	35.0	40.0	30.0	41.0
45	35.98072779608765	38.0	35.0	40.0	30.0	41.0
46	35.88120725922519	38.0	35.0	40.0	30.0	41.0
47	35.64488004905342	38.0	34.0	40.0	29.0	41.0
48	35.5980961992114	38.0	34.0	40.0	29.0	41.0
49	35.6371047409792	38.0	34.0	40.0	29.0	41.0
50	35.50678103948971	38.0	34.0	40.0	28.0	41.0
51	34.61977849314019	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	9.0
11	10.0
12	7.0
13	2.0
14	5.0
15	17.0
16	29.0
17	46.0
18	113.0
19	176.0
20	328.0
21	576.0
22	854.0
23	1250.0
24	1778.0
25	2615.0
26	3524.0
27	4424.0
28	5309.0
29	6933.0
30	8725.0
31	11290.0
32	15080.0
33	20765.0
34	36400.0
35	46880.0
36	40512.0
37	56952.0
38	94044.0
39	110997.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.160547763215042	4.735443652436064	53.74458155557259	15.359427028776304
2	19.36290164618516	6.59772787273362	53.36497108743602	20.6743993936452
3	20.080606014153958	6.521720616914914	52.073273549474976	21.324399819456154
4	19.651175664052186	4.270883898384473	52.03963448387454	24.038305953688802
5	19.027788423051703	5.579188063667254	51.18247702749888	24.21054648578217
6	21.10404264922545	8.095730819345444	52.18270696541564	18.617519566013474
7	80.8974817540005	2.2516883404443764	11.444521090416698	5.406308815138431
8	81.2543113359393	4.2630063956805735	8.478534869659265	6.004147398720864
9	75.71344625839912	4.786328061793686	10.965696669306695	8.534529010500497
10	43.252812481370775	20.767439087742606	19.5998654437376	16.379882987149024
11	30.02137571003977	24.693203205504886	25.599328921931818	19.686092162523526
12	27.92872776202277	21.086797305468263	31.430384166645375	19.554090765863588
13	23.629740340478442	26.31958815564242	31.323718521925002	18.72695298195413
14	18.038629570016095	29.422898410021887	32.16064995784472	20.3778220621173
15	16.85764288086661	25.315313013634466	38.0924094938811	19.734634611617828
16	19.116995818536402	24.46262657230696	33.353985164746256	23.06639244441038
17	18.853631741651977	25.42495933505361	29.66475903357945	26.05664988971496
18	20.27498871600964	24.741958560077666	32.942225969358645	22.04082675455405
19	22.90117779310697	24.993612835645486	29.34284595011199	22.762363421135554
20	23.866917043509364	26.95340776508861	30.003491649847135	19.17618354155489
21	22.91011982320329	28.476959369118486	29.79739914667484	18.81552166100338
22	20.111051497577133	25.209711896306512	30.626453079890652	24.052783526225696
23	20.577314495456598	27.272765982814267	28.003244679492095	24.14667484223704
24	21.576267000502458	23.904175502244023	31.34011224376826	23.179445253485262
25	19.5355679892355	26.716018156579207	28.642386925900375	25.10602692828492
26	17.97348049360006	28.591076705585788	28.698168161263126	24.737274639551025
27	18.210870102109467	30.098873304207864	29.611319758480025	22.078936835202644
28	15.989414339609787	28.29769295623515	32.030990521448096	23.681902182706967
29	19.00117523824123	24.941450993416964	31.313286153479304	24.744087614862504
30	19.188957870263916	28.29641552336425	28.804833805983492	23.709792800388342
31	22.96015261064698	28.269802338553774	26.023862446028463	22.746182604770787
32	24.703422668472104	27.313643834683155	25.558238164584452	22.42469533226029
33	22.58160666990283	27.339405397579693	25.913577408173865	24.16541052434361
34	19.53790994949882	26.246348671044	29.635803888505656	24.57993749095152
35	20.292021154288342	27.165035810701482	26.678759697844544	25.86418333716563
36	21.58606065251271	28.829743746966095	26.203767575347246	23.380428025173945
37	20.4421195166194	30.276862284220297	29.00496495575824	20.27605324340206
38	21.84623114721988	29.162089198879265	25.922519438270186	23.069160215630667
39	21.87497338681519	26.111579503163775	28.364971087436018	23.64847602258501
40	22.122582458291816	24.51798199671274	28.25489895505991	25.104536589935535
41	19.788286792195738	26.203767575347246	27.27851443073333	26.72943120172368
42	21.233063369186617	26.26018752714545	26.73645708251365	25.77029202115429
43	21.438304250444972	27.197610348909496	26.29595564753072	25.06812975311481
44	20.52281069296475	25.151375795201965	27.961515205709276	26.364298306124013
45	20.512804135476014	25.575909319298606	25.931887279323472	27.97939926590191
46	21.76660449826695	26.229316232765303	28.562760276947447	23.441318992020303
47	18.76357272425334	25.7438917418223	30.64838234417448	24.84415318974988
48	19.95712083663337	25.15691133764254	29.916413309147273	24.96955451657682
49	20.889221021435322	23.228200608058046	32.11338494162132	23.769193428885313
50	20.85579486131337	24.076841845294364	28.834640572971225	26.23272272042104
51	19.63605937507984	23.924188617221496	26.512480519148717	29.927271488549945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82.0
1	280.5
2	479.0
3	1331.0
4	2183.0
5	1411.0
6	639.0
7	728.5
8	818.0
9	938.0
10	1058.0
11	1162.5
12	1267.0
13	1353.0
14	1439.0
15	1301.5
16	1164.0
17	1241.5
18	1319.0
19	1395.0
20	1471.0
21	1448.0
22	1425.0
23	1638.0
24	1851.0
25	2446.5
26	3297.5
27	3553.0
28	4255.0
29	4957.0
30	6296.0
31	7635.0
32	8898.5
33	10162.0
34	9904.0
35	9646.0
36	12184.5
37	14723.0
38	16075.0
39	17427.0
40	20697.0
41	23967.0
42	27449.0
43	30931.0
44	34422.5
45	37914.0
46	46450.0
47	54986.0
48	56574.0
49	58162.0
50	54749.0
51	51336.0
52	43031.0
53	34726.0
54	29700.5
55	24675.0
56	21638.0
57	18601.0
58	16344.0
59	14087.0
60	12408.5
61	10730.0
62	9218.0
63	7706.0
64	6568.5
65	5431.0
66	4514.0
67	3597.0
68	3285.0
69	2973.0
70	2163.0
71	1353.0
72	1257.0
73	1161.0
74	788.0
75	359.5
76	304.0
77	199.0
78	94.0
79	107.5
80	121.0
81	80.5
82	40.0
83	30.5
84	21.0
85	13.0
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	3.0
92	5.0
93	6.0
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	469692.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.653987483038673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.92455296623712	20.322712529346717
2	12.146374636515473	6.9608413359802555
3	5.220212712391442	4.487397291589913
4	2.6844826166557754	3.07684525184358
5	1.4976947821592825	2.1457463770702208
6	1.0157555812067325	1.7463268625834631
7	0.6792527615273966	1.3624310088637832
8	0.46287539417719664	1.0610580600767987
9	0.3376163186601389	0.8706648392061493
>10	3.719544388715415	26.30324250020578
>50	1.2035767695640713	22.57111984199333
>100	0.09911808690527646	4.9121409082988565
>500	0.0029809950948955326	0.6077447735086094
>1k	0.005961990189791065	3.571728419432538
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2880	0.6131677780332644	No Hit
TCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2647	0.5635608015465454	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC	2146	0.45689515682617543	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG	2110	0.4492305596007597	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	2085	0.44390792263866535	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	1805	0.3842943886632091	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	1668	0.35512633811093225	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1385	0.29487408770002466	No Hit
TCCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	877	0.18671810463026836	No Hit
TGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	683	0.1454144418044165	No Hit
GCCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	653	0.13902727744990334	No Hit
TTCCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	633	0.13476916788022789	No Hit
TTGTTGGGTGTTATGTATTGTGGTTTTTTTGTTTGTTTTATTTTGTTCTAA	496	0.10560111732795109	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGAT	492	0.10474949541401599	No Hit
GCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	476	0.10134300775827562	No Hit
TTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	473	0.10070429132282431	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1290547848377235E-4	0.0	0.0	0.5043730785280567	0.0
2	2.1290547848377235E-4	0.0	0.0	2.2757466595130427	0.0
3	2.1290547848377235E-4	0.0	0.0	3.2604344975004897	0.0
4	2.1290547848377235E-4	0.0	0.0	4.356684806213433	0.0
5	2.1290547848377235E-4	0.0	0.0	6.3064731781678205	0.0
6	2.1290547848377235E-4	0.0	0.0	7.2675285080435685	0.0
7	2.1290547848377235E-4	0.0	0.0	8.23497100227383	0.0
8	2.1290547848377235E-4	0.0	0.0	9.604591945359939	0.0
9	2.1290547848377235E-4	0.0	0.0	10.227127564446489	0.0
10	2.1290547848377235E-4	0.0	0.0	11.872035291212114	0.0
11	2.1290547848377235E-4	0.0	0.0	14.17396932457866	0.0
12	2.1290547848377235E-4	0.0	0.0	16.482503427778205	0.0
13	2.1290547848377235E-4	0.0	0.0	17.09588411128995	0.0
14	2.1290547848377235E-4	0.0	0.0	17.30687344046737	0.0
15	2.1290547848377235E-4	0.0	0.0	17.701174386619318	0.0
16	2.1290547848377235E-4	0.0	0.0	18.531718658184513	0.0
17	2.1290547848377235E-4	0.0	0.0	19.79339652367935	0.0
18	4.258109569675447E-4	0.0	0.0	21.13576556551953	0.0
19	4.258109569675447E-4	0.0	0.0	22.142169762312324	0.0
20	4.258109569675447E-4	0.0	0.0	23.02572749801998	0.0
21	4.258109569675447E-4	0.0	0.0	24.126023010824113	0.0
22	4.258109569675447E-4	0.0	0.0	25.295512804135477	0.0
23	4.258109569675447E-4	0.0	0.0	26.320865588513325	0.0
24	4.258109569675447E-4	0.0	0.0	27.163545472352094	0.0
25	6.38716435451317E-4	0.0	0.0	27.89530160190082	0.0
26	6.38716435451317E-4	0.0	0.0	28.56126993859806	0.0
27	6.38716435451317E-4	0.0	0.0	29.202115428834215	0.0
28	6.38716435451317E-4	0.0	0.0	29.86382565596178	0.0
29	6.38716435451317E-4	0.0	0.0	30.567265356872163	0.0
30	6.38716435451317E-4	0.0	0.0	31.386951449034687	0.0
31	6.38716435451317E-4	0.0	0.0	32.082726552719656	0.0
32	6.38716435451317E-4	0.0	0.0	32.74252063054087	0.0
33	6.38716435451317E-4	0.0	0.0	33.36164976197168	0.0
34	6.38716435451317E-4	0.0	0.0	34.00568883438508	0.0
35	6.38716435451317E-4	0.0	0.0	34.65121824514788	0.0
36	6.38716435451317E-4	0.0	0.0	35.23074695758071	0.0
37	6.38716435451317E-4	0.0	0.0	35.87478602999413	0.0
38	6.38716435451317E-4	0.0	0.0	36.48688928063497	0.0
39	6.38716435451317E-4	0.0	0.0	37.22396804714579	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.02902E-4	45.000004	34
AACGTAG	20	7.02902E-4	45.000004	38
CGGGTAG	40	6.7993824E-9	45.000004	6
GCCTTCG	20	7.02902E-4	45.000004	1
GTACCCC	30	2.1627493E-6	45.000004	36
TACCCGT	30	2.1627493E-6	45.000004	14
TGCCCGT	20	7.02902E-4	45.000004	13
CGAGTCG	20	7.02902E-4	45.000004	28
CATGCGT	20	7.02902E-4	45.000004	14
CGGCCCC	20	7.02902E-4	45.000004	21
TAGCGAT	20	7.02902E-4	45.000004	44
TTGTCGA	20	7.02902E-4	45.000004	10
GTTAGCG	30	2.1627493E-6	45.000004	1
TAACGGA	20	7.02902E-4	45.000004	29
CGACAAT	20	7.02902E-4	45.000004	20
TGACGAA	20	7.02902E-4	45.000004	36
TACGCGG	40	6.7993824E-9	45.000004	2
CACTCCA	20	7.02902E-4	45.000004	32
TATCTCG	20	7.02902E-4	45.000004	17
CTAGTCG	20	7.02902E-4	45.000004	23
>>END_MODULE