##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934387.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2286739
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15826729679251	33.0	31.0	34.0	30.0	34.0
2	32.369102026947544	34.0	31.0	34.0	30.0	34.0
3	32.37201184743864	34.0	31.0	34.0	30.0	34.0
4	35.942318296928505	37.0	35.0	37.0	35.0	37.0
5	35.92396727392151	37.0	35.0	37.0	35.0	37.0
6	35.60166158009287	37.0	35.0	37.0	33.0	37.0
7	35.930833383258864	37.0	35.0	37.0	35.0	37.0
8	36.03091738934789	37.0	35.0	37.0	35.0	37.0
9	37.81982683638142	39.0	38.0	39.0	35.0	39.0
10	37.305537711124884	39.0	37.0	39.0	34.0	39.0
11	37.167070662633556	39.0	37.0	39.0	34.0	39.0
12	36.95119775365706	39.0	37.0	39.0	33.0	39.0
13	36.88074546329948	39.0	37.0	39.0	33.0	39.0
14	37.874287795852524	40.0	37.0	41.0	33.0	41.0
15	37.953369842382536	40.0	37.0	41.0	33.0	41.0
16	38.0386248714873	40.0	37.0	41.0	33.0	41.0
17	38.015786672637326	40.0	37.0	41.0	33.0	41.0
18	37.89715004641982	39.0	37.0	41.0	33.0	41.0
19	37.81240491372212	39.0	37.0	41.0	33.0	41.0
20	37.639648862419364	39.0	35.0	41.0	33.0	41.0
21	37.4874955121682	39.0	35.0	41.0	33.0	41.0
22	37.60675966955564	39.0	35.0	41.0	33.0	41.0
23	37.55683967431351	39.0	35.0	41.0	33.0	41.0
24	37.49777827727607	39.0	35.0	41.0	33.0	41.0
25	37.41585637888714	39.0	35.0	41.0	33.0	41.0
26	37.36719319520068	39.0	35.0	41.0	33.0	41.0
27	37.3082306288562	39.0	35.0	41.0	33.0	41.0
28	37.175129299845764	39.0	35.0	41.0	33.0	41.0
29	37.07653606292629	39.0	35.0	41.0	32.0	41.0
30	36.98487540554475	39.0	35.0	41.0	32.0	41.0
31	36.79437924485479	39.0	35.0	41.0	31.0	41.0
32	36.507643417110565	39.0	35.0	41.0	31.0	41.0
33	36.26449760991525	39.0	35.0	41.0	30.0	41.0
34	35.911119283836065	39.0	35.0	41.0	29.0	41.0
35	35.729879973184524	39.0	35.0	41.0	27.0	41.0
36	35.52642955754898	39.0	35.0	40.0	26.0	41.0
37	35.44909147917624	38.0	35.0	40.0	25.0	41.0
38	35.344872326924936	38.0	35.0	40.0	25.0	41.0
39	35.2619769899407	38.0	35.0	40.0	25.0	41.0
40	35.12228155465053	38.0	34.0	40.0	24.0	41.0
41	34.96909004481928	38.0	34.0	40.0	23.0	41.0
42	34.89983028233655	38.0	34.0	40.0	23.0	41.0
43	34.87460265469737	38.0	34.0	40.0	23.0	41.0
44	34.75771436967664	38.0	34.0	40.0	23.0	41.0
45	34.784013391996204	38.0	34.0	40.0	23.0	41.0
46	34.67837168999173	38.0	34.0	40.0	23.0	41.0
47	34.45667782812118	38.0	34.0	40.0	23.0	41.0
48	34.314693543950575	37.0	33.0	40.0	22.0	41.0
49	34.3138631037473	37.0	33.0	40.0	23.0	41.0
50	34.197564741756715	37.0	33.0	40.0	22.0	41.0
51	33.25162075776903	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	20.0
10	40.0
11	33.0
12	22.0
13	22.0
14	40.0
15	72.0
16	157.0
17	316.0
18	636.0
19	1279.0
20	2285.0
21	3680.0
22	6107.0
23	9854.0
24	16410.0
25	28064.0
26	38779.0
27	41434.0
28	39611.0
29	40730.0
30	45221.0
31	54966.0
32	69423.0
33	96825.0
34	154033.0
35	167545.0
36	191899.0
37	281671.0
38	465424.0
39	529997.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.97564348183155	4.233714472880377	54.32045371159542	18.470188333692654
2	25.284826995997356	4.1416182607634715	49.63093733040806	20.942617412831112
3	19.218459124543728	4.2029282747178405	55.07930725806487	21.499305342673562
4	17.576513979076754	4.5119709770113685	54.11028543266197	23.80122961124991
5	17.436139410750418	5.380675276015321	54.05321726703397	23.12996804620029
6	20.276647225590676	5.876927799805749	56.521710610611876	17.324714363991692
7	79.48038669913794	1.902709491551069	14.537688822379815	4.079214986931171
8	81.84550138865869	2.0801674349368247	12.382829872582747	3.69150130382173
9	76.85245233496258	4.490411892218569	14.190338293963586	4.46679747885526
10	38.10841552096675	27.61351426638545	21.032483374797035	13.245586837850757
11	27.472308820551888	23.856942134629268	30.758123248870987	17.912625795947854
12	24.137166506540535	21.287606499911007	35.19439691193442	19.380830081614036
13	23.856067526726925	21.152304657418274	36.40161819954092	18.590009616313885
14	20.449644668674473	22.880529872451557	37.28938895081599	19.38043650805798
15	18.4589058917524	23.472333309573152	38.082833239823174	19.985927558851273
16	22.469376697559277	24.22886914510139	34.159779493855666	19.141974663483676
17	23.82619966686185	23.700562241690022	32.245962481944815	20.227275609503316
18	23.99849742362377	22.44462529392292	33.97580572159743	19.58107156085587
19	23.488557286161647	25.084716707940874	31.783732205555598	19.642993800341884
20	23.541777177019327	26.983315542350923	31.514221780448054	17.9606855001817
21	23.24051848505667	25.86792808449062	33.408316384161026	17.483237046291684
22	22.40308141856154	22.446768083283665	34.3536800658055	20.7964704323493
23	21.002353132561257	24.81153292964348	33.28311626294037	20.90299767485489
24	21.38866744302695	24.93472145268874	32.950240495307945	20.726370608976364
25	21.946492363142447	25.90291240058441	30.3540543979877	21.796540838285434
26	20.459352816390503	25.52779307126874	31.676461546333005	22.336392566007753
27	20.06595418191582	24.44979510123368	34.370122694369584	21.114128022480923
28	18.624381706876036	25.000579427735303	33.24410875049579	23.130930114892866
29	19.761109597553546	24.595592238554552	33.059566483101044	22.583731680790855
30	22.61753527621648	23.595653023803767	33.38588269146589	20.40092900851387
31	22.18801533537496	25.440069898663552	31.77621057759543	20.595704188366053
32	22.118046703187378	25.620457778522166	30.89464954242701	21.36684597586345
33	23.267981173190293	24.3696810173789	29.710780285813115	22.651557523617694
34	21.080062044684592	25.117077200327625	31.645937730541178	22.156923024446602
35	20.13089381866492	26.105078017211408	30.850525573753718	22.913502590369955
36	22.286714837154566	27.29660009297082	29.270808780538577	21.145876289336037
37	20.88546178641288	28.104169299600873	28.90469791261705	22.1056710013692
38	21.585410490659406	28.16722852935993	27.969873256195832	22.27748772378483
39	22.68181895703882	24.78931788892392	27.40238391875942	25.12647923527783
40	24.85631285424353	24.840394990420858	28.31958522594839	21.98370692938722
41	21.398681703508796	25.547471749071494	28.477758065087443	24.576088482332263
42	20.45620422794206	25.5870477566526	29.680125278835934	24.27662273656941
43	20.176723272747786	24.615970602679184	31.017400761521102	24.189905363051928
44	21.525849692509727	24.336883221040967	29.166686709764427	24.97058037668488
45	21.718919386952336	23.58113453262484	28.444216852032522	26.255729228390294
46	21.467425884633094	24.46370136688096	29.449622366172967	24.61925038231298
47	19.437679595266445	25.734200536222108	31.4669054929312	23.361214375580246
48	19.652658217662793	26.695175968923436	30.512096045941405	23.140069767472372
49	22.176645432644477	24.03466246038573	30.031017969256656	23.757674137713135
50	20.978388876037013	24.232936071847288	29.045072480943386	25.743602571172314
51	20.453274291469207	24.159206625679623	27.96606871182063	27.421450371030538
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	410.0
1	1196.5
2	1983.0
3	21326.0
4	40669.0
5	27204.0
6	13739.0
7	13902.5
8	14066.0
9	14652.5
10	15239.0
11	15374.5
12	15510.0
13	15465.5
14	15421.0
15	14955.0
16	14489.0
17	14145.5
18	13802.0
19	13280.0
20	12758.0
21	12642.0
22	12526.0
23	12939.0
24	13352.0
25	14548.0
26	17454.5
27	19165.0
28	21787.5
29	24410.0
30	29746.0
31	35082.0
32	39175.5
33	43269.0
34	50377.5
35	57486.0
36	62294.0
37	67102.0
38	76276.0
39	85450.0
40	95424.5
41	105399.0
42	115712.5
43	126026.0
44	138934.5
45	151843.0
46	174547.0
47	197251.0
48	214741.5
49	232232.0
50	224184.5
51	216137.0
52	190145.0
53	164153.0
54	148065.0
55	131977.0
56	118806.5
57	105636.0
58	98254.5
59	90873.0
60	81029.5
61	71186.0
62	61490.5
63	51795.0
64	44422.0
65	37049.0
66	31452.5
67	25856.0
68	21958.0
69	18060.0
70	14766.5
71	11473.0
72	9614.5
73	7756.0
74	6157.5
75	3810.0
76	3061.0
77	2259.0
78	1457.0
79	1076.5
80	696.0
81	513.5
82	331.0
83	261.5
84	192.0
85	122.0
86	52.0
87	31.0
88	10.0
89	7.0
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2286739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.379674356073856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47093241575277	24.113445406152596
2	10.778372154701229	6.980003609155998
3	4.065054710730234	3.9487544331920685
4	2.0725484929771576	2.6843378115908796
5	1.265751828559583	2.049231601218216
6	0.8020968359266362	1.5582980609605006
7	0.6079492245187019	1.3779638540460237
8	0.4749004203706826	1.2301696770532227
9	0.38326250109686316	1.1168923480569743
>10	4.3333532358794145	32.554492803798944
>50	0.6537802377565117	13.686888313657459
>100	0.08528036871099598	4.8375152552422165
>500	0.0034273331730973705	0.7079376657631009
>1k	0.003153146519249581	1.9855369859491938
>5k	0.0	0.0
>10k+	1.3709332692389483E-4	1.168532174162771
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26324	1.1511589210661997	No Hit
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT	4953	0.2165966470156848	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	4486	0.19617455249593416	TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	4241	0.18546060569221062	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	2992	0.13084134219077911	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCC	2921	0.12773648413745511	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.31191185351717E-4	0.0	0.0	0.23846184457430428	0.0
2	1.31191185351717E-4	0.0	0.0	0.8247115215160103	0.0
3	1.31191185351717E-4	0.0	0.0	1.2578173547571454	0.0
4	1.31191185351717E-4	0.0	0.0	1.837201359665445	0.0
5	1.31191185351717E-4	0.0	0.0	2.817505627008592	0.0
6	1.31191185351717E-4	0.0	0.0	3.7561348278050097	0.0
7	1.74921580468956E-4	0.0	0.0	4.553252470002042	0.0
8	1.74921580468956E-4	0.0	0.0	5.688318605665097	0.0
9	1.74921580468956E-4	0.0	0.0	6.38485633909248	0.0
10	1.74921580468956E-4	0.0	0.0	7.43591638573532	0.0
11	1.74921580468956E-4	0.0	0.0	9.148835962477571	0.0
12	1.74921580468956E-4	0.0	0.0	10.484974454889691	0.0
13	1.74921580468956E-4	0.0	0.0	10.990847665605912	0.0
14	1.74921580468956E-4	0.0	0.0	11.215359514137818	0.0
15	1.74921580468956E-4	0.0	0.0	11.460861952325997	0.0
16	1.74921580468956E-4	0.0	0.0	11.971501776109998	0.0
17	1.74921580468956E-4	0.0	0.0	12.719291532614784	0.0
18	1.74921580468956E-4	0.0	0.0	13.546495686652477	0.0
19	1.74921580468956E-4	0.0	0.0	14.1746390821165	0.0
20	1.74921580468956E-4	0.0	0.0	14.82447275355867	0.0
21	1.74921580468956E-4	0.0	0.0	15.707520622161077	0.0
22	1.74921580468956E-4	0.0	0.0	16.687606237528637	0.0
23	2.1865197558619502E-4	0.0	0.0	17.650330886034656	0.0
24	2.1865197558619502E-4	0.0	0.0	18.359419242860685	0.0
25	2.62382370703434E-4	0.0	0.0	18.995390379050693	0.0
26	3.0611276582067303E-4	0.0	0.0	19.562092569374993	0.0
27	3.0611276582067303E-4	0.0	0.0	20.17256888521165	0.0
28	3.0611276582067303E-4	0.0	0.0	20.7926658879741	0.0
29	3.0611276582067303E-4	0.0	0.0	21.49414515604973	0.0
30	3.0611276582067303E-4	0.0	0.0	22.291875023778402	0.0
31	3.0611276582067303E-4	0.0	0.0	22.95819505417977	0.0
32	3.0611276582067303E-4	0.0	0.0	23.594603494320953	0.0
33	3.0611276582067303E-4	0.0	0.0	24.227469772457635	0.0
34	3.0611276582067303E-4	0.0	0.0	24.884300307118565	0.0
35	3.0611276582067303E-4	0.0	0.0	25.609568910137973	0.0
36	3.0611276582067303E-4	0.0	0.0	26.246196002254738	0.0
37	3.0611276582067303E-4	0.0	0.0	26.89764769831625	0.0
38	3.49843160937912E-4	0.0	0.0	27.642332596767712	0.0
39	3.49843160937912E-4	0.0	0.0	28.762268015720203	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAG	20	7.034754E-4	45.0	44
GATAGCG	20	7.034754E-4	45.0	9
CGTTTTT	16545	0.0	44.07525	1
TACGGGT	50	1.0822987E-9	40.500004	4
CGAATAT	190	0.0	39.078945	14
TATTGCG	125	0.0	37.8	1
GTTTTTT	19725	0.0	37.73384	2
ACGCAAC	30	1.1402752E-4	37.500004	17
TTCGTAC	60	1.5643309E-10	37.500004	33
TCGTACA	60	1.5643309E-10	37.500004	34
TTTTGCG	1955	0.0	37.404095	1
TGGGCGA	2995	0.0	37.337227	6
CGGGATA	585	0.0	37.30769	6
TGCGGGA	2890	0.0	37.29239	4
TCGTTTG	745	0.0	37.14765	1
TTTGTCG	530	0.0	36.933964	1
TTTGGGA	12630	0.0	36.876488	4
TACGGGA	330	0.0	36.81818	4
GTTTGCG	435	0.0	36.724136	1
TTGGGAC	4965	0.0	36.072506	5
>>END_MODULE