FastQCFastQC Report
Sat 14 Jan 2017
SRR2934386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186760
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48700.410360982844046No Hit
CTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGCT14080.1186423539721595Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGC13190.11114294381340793No Hit
TCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGC12170.1025481141932657No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG253.890883E-545.011
ATAGCGG253.890883E-545.02
CGTCGCA207.03338E-445.037
TACGTCT253.890883E-545.014
TATTGCG650.041.538461
CGTTTTT26450.040.4914931
TTATCGG403.4583536E-739.3752
TGTTTCG3500.039.2142871
CGGGTAT1550.039.1935466
TTCGGGC4550.039.0659334
CGGGATA5850.038.8461576
GCGGGAT11600.038.5991365
TCTTGCG3150.038.571431
TGCGGGA20600.038.2281534
TTTGGGA80150.037.673114
TTAATCG601.5643309E-1037.49999620
CGTAGAT601.5643309E-1037.49999616
CAAGGCG301.1399444E-437.49999642
TTTTGCG10950.037.191781
CGGGCTC1400.036.9642836
Produced by FastQC (version 0.11.5)