##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934386.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1186760
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.735350871279785	34.0	31.0	34.0	31.0	34.0
2	32.939597728268566	34.0	31.0	34.0	31.0	34.0
3	32.9712039502511	34.0	33.0	34.0	31.0	34.0
4	36.33872813374229	37.0	37.0	37.0	35.0	37.0
5	36.292796353095824	37.0	37.0	37.0	35.0	37.0
6	35.98318025548552	37.0	36.0	37.0	35.0	37.0
7	36.22063433213118	37.0	36.0	37.0	35.0	37.0
8	36.31817637938589	37.0	37.0	37.0	35.0	37.0
9	38.14589217701978	39.0	39.0	39.0	37.0	39.0
10	37.819009740806905	39.0	38.0	39.0	35.0	39.0
11	37.706278438774476	39.0	37.0	39.0	35.0	39.0
12	37.60866898109138	39.0	37.0	39.0	35.0	39.0
13	37.62072702147022	39.0	37.0	39.0	35.0	39.0
14	38.98271006774748	40.0	38.0	41.0	35.0	41.0
15	39.02978108463379	40.0	38.0	41.0	35.0	41.0
16	39.045787690855775	40.0	38.0	41.0	35.0	41.0
17	39.007081465502715	40.0	38.0	41.0	35.0	41.0
18	38.934242812363074	40.0	38.0	41.0	35.0	41.0
19	38.93630051568978	40.0	38.0	41.0	35.0	41.0
20	38.8680230206613	40.0	38.0	41.0	35.0	41.0
21	38.77401749300617	40.0	38.0	41.0	35.0	41.0
22	38.78771444942532	40.0	38.0	41.0	35.0	41.0
23	38.72844972867303	40.0	38.0	41.0	35.0	41.0
24	38.68209747547946	40.0	38.0	41.0	35.0	41.0
25	38.63234099565203	40.0	38.0	41.0	35.0	41.0
26	38.600695170042805	40.0	38.0	41.0	35.0	41.0
27	38.56162745626749	40.0	38.0	41.0	35.0	41.0
28	38.47159071758401	40.0	38.0	41.0	35.0	41.0
29	38.41854545148134	40.0	38.0	41.0	35.0	41.0
30	38.37800060669385	40.0	38.0	41.0	35.0	41.0
31	38.285364353365466	40.0	37.0	41.0	34.0	41.0
32	38.17029812261957	40.0	37.0	41.0	34.0	41.0
33	38.06930634669183	40.0	37.0	41.0	34.0	41.0
34	37.93778691563585	40.0	37.0	41.0	34.0	41.0
35	37.866964676935524	40.0	37.0	41.0	34.0	41.0
36	37.73113350635343	40.0	37.0	41.0	33.0	41.0
37	37.69102345882908	40.0	37.0	41.0	33.0	41.0
38	37.65556641612457	40.0	37.0	41.0	33.0	41.0
39	37.59547001921197	40.0	36.0	41.0	33.0	41.0
40	37.50554282247464	40.0	36.0	41.0	33.0	41.0
41	37.42287657150561	40.0	36.0	41.0	33.0	41.0
42	37.32279062320941	40.0	36.0	41.0	33.0	41.0
43	37.26371886480839	40.0	36.0	41.0	33.0	41.0
44	37.14542957295494	40.0	35.0	41.0	33.0	41.0
45	37.13226600155044	39.0	35.0	41.0	33.0	41.0
46	37.051189794061145	39.0	35.0	41.0	32.0	41.0
47	36.920229026930464	39.0	35.0	41.0	32.0	41.0
48	36.8396811486737	39.0	35.0	41.0	32.0	41.0
49	36.800708652128485	39.0	35.0	41.0	32.0	41.0
50	36.707054501331356	39.0	35.0	41.0	32.0	41.0
51	35.82728100037076	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	14.0
10	16.0
11	17.0
12	17.0
13	16.0
14	23.0
15	31.0
16	63.0
17	89.0
18	133.0
19	216.0
20	418.0
21	699.0
22	1116.0
23	1745.0
24	2698.0
25	4529.0
26	6709.0
27	8108.0
28	8606.0
29	8921.0
30	10271.0
31	12701.0
32	17120.0
33	25913.0
34	53512.0
35	72571.0
36	80372.0
37	136378.0
38	278231.0
39	455311.0
40	188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.503421079240958	4.076308604941184	57.72228588762681	16.69798442819104
2	20.476844517846914	4.26202433516465	54.31982877751188	20.94130236947656
3	19.432825508106106	4.070831507634231	54.27146179513971	22.22488118911996
4	18.53247497387846	4.520459065017358	52.04599076477131	24.901075196332872
5	18.205450133135596	5.438757625804712	51.84805689440156	24.507735346658126
6	21.849152313862955	5.961694024065523	54.08599885402272	18.103154808048807
7	85.84246182884492	1.9481613805655735	8.546546900805554	3.6628298897839495
8	88.43692069163099	2.3107452222858877	6.285432606424214	2.9669014796589033
9	82.76247935555631	5.224392463514105	7.946931140247396	4.0661970406821935
10	37.671306751154404	34.17750851056658	14.977333243452762	13.17385149482625
11	25.301830193130876	24.938993562304088	30.88417203141326	18.875004213151776
12	23.780208298223734	22.420118642353973	33.01543698810206	20.784236071320233
13	22.55199029289831	22.631281809295896	33.41223162223196	21.40449627557383
14	20.156139404765916	24.768697967575584	33.39158717853652	21.683575449121978
15	19.1322592605076	26.162408574606495	34.03097509184671	20.6743570730392
16	21.99518015436988	24.877481546395227	32.97911961980519	20.148218679429707
17	22.03798577639961	24.517594121810642	31.00449964609525	22.439920455694494
18	21.271023627355152	25.28910647477165	31.9929893154471	21.4468805824261
19	23.774815463952272	25.876082780006065	30.472968418214297	19.876133337827362
20	25.32373858235869	26.040142910108194	29.805268124978934	18.830850382554182
21	25.3677238868853	25.67890727695574	30.037328524722774	18.91604031143618
22	23.873487478512924	23.7004112036132	30.441032727762984	21.98506859011089
23	22.068404732212073	25.45181839630591	30.36190973743638	22.11786713404564
24	21.860864875796285	24.837709393643195	31.539569921466853	21.761855809093667
25	21.430702079611716	26.37441437190333	29.291095082409246	22.903788466075703
26	20.49369712494523	28.193316256024808	29.44276854629411	21.870218072735852
27	20.443055040614784	27.773602076241193	30.4138157672992	21.36952711584482
28	19.298425966497017	27.072617883986656	32.045148134416394	21.583808015099937
29	20.38145876167043	25.565067916006605	31.803144696484548	22.250328625838417
30	21.79699349489366	25.64275843472985	30.590768141831543	21.969479928544946
31	21.294617277292797	27.88356533755772	28.845090835552263	21.976726549597224
32	22.063686002224543	27.386076376015367	28.492112979877987	22.0581246418821
33	22.51424045299808	27.314284269776536	28.17486265125215	21.99661262597324
34	21.655094543125823	26.525835046681724	28.93314570764097	22.885924702551485
35	21.31492466884627	26.130557147190668	29.250396036266814	23.304122147696248
36	21.255182176682734	28.6833900704439	28.44526273214466	21.616165020728705
37	20.740587818935587	29.16874515487546	28.564663453436246	21.526003572752707
38	21.182631703124475	27.849354545148135	27.624203714314604	23.343810037412787
39	22.019279382520477	26.34559641376521	28.78779197141798	22.847332232296335
40	22.208281371128113	25.845663824193604	28.77624793555563	23.169806869122652
41	20.655819205231047	27.065371262934374	28.942751693687015	23.33605783814756
42	20.85493275809768	27.74520543328053	28.598958508881324	22.80090329974047
43	20.755081061040144	27.320603997438404	29.069230509959894	22.85508443156156
44	20.859651488085206	27.089049175907515	28.557753884525937	23.493545451481342
45	21.67582324985675	25.814992079274663	28.072483063129866	24.436701607738716
46	20.918888402035797	26.813677575920998	29.01285853921602	23.254575482827196
47	20.095217230105497	27.302150392665748	29.7625467659847	22.84008561124406
48	20.240065388115543	26.397081128450573	30.456452863257944	22.90640062017594
49	20.47583336142101	26.291162492837643	29.61609761030031	23.616906535441032
50	20.456789915399913	25.69356904513128	29.20084937139776	24.648791668071052
51	20.299723617243586	25.627338299234893	28.21707843203344	25.855859651488082
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	244.0
1	775.0
2	1306.0
3	5083.0
4	8860.0
5	6017.0
6	3174.0
7	3122.0
8	3070.0
9	3187.5
10	3305.0
11	3309.0
12	3313.0
13	3243.5
14	3174.0
15	3181.0
16	3188.0
17	3213.5
18	3239.0
19	4093.0
20	4947.0
21	5516.5
22	6086.0
23	7125.5
24	8165.0
25	9832.0
26	14012.5
27	16526.0
28	19087.5
29	21649.0
30	24658.0
31	27667.0
32	31418.5
33	35170.0
34	39337.5
35	43505.0
36	46140.5
37	48776.0
38	52222.0
39	55668.0
40	60422.5
41	65177.0
42	68602.0
43	72027.0
44	75399.0
45	78771.0
46	85842.5
47	92914.0
48	97686.5
49	102459.0
50	99313.5
51	96168.0
52	85588.5
53	75009.0
54	67112.5
55	59216.0
56	54895.0
57	50574.0
58	48774.0
59	46974.0
60	43681.0
61	40388.0
62	35493.5
63	30599.0
64	26488.5
65	22378.0
66	18809.0
67	15240.0
68	12555.0
69	9870.0
70	8187.0
71	6504.0
72	5346.5
73	4189.0
74	3485.5
75	2112.0
76	1442.0
77	1115.0
78	788.0
79	630.5
80	473.0
81	291.0
82	109.0
83	74.5
84	40.0
85	58.0
86	76.0
87	56.5
88	37.0
89	30.5
90	24.0
91	12.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1186760.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.669313396619604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.46221496159535	20.326490485822195
2	10.244633514078618	5.669239506691073
3	3.6315744337918647	3.0144951339521557
4	1.7397800300383002	1.9255407556923998
5	1.0530399632766574	1.456844638153332
6	0.7413498468631786	1.2307584749635945
7	0.537357223605475	1.0407813798114098
8	0.4519969303426961	1.000515577596968
9	0.38182554406094504	0.9508365577311371
>10	7.094281328336985	49.810487660682334
>50	0.6098174090525962	10.224300977081292
>100	0.04873581516727283	2.17131937677335
>500	0.0018507271582135524	0.32961107790528993
>1k	0.001542272631844627	0.8487783971434771
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4870	0.410360982844046	No Hit
CTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGCT	1408	0.1186423539721595	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGC	1319	0.11114294381340793	No Hit
TCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGC	1217	0.1025481141932657	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.426303549159054E-5	0.0	0.0	0.1238666621726381	0.0
2	8.426303549159054E-5	0.0	0.0	0.45864370218072736	0.0
3	8.426303549159054E-5	0.0	0.0	0.7495197006976979	0.0
4	8.426303549159054E-5	0.0	0.0	1.1180019549024234	0.0
5	8.426303549159054E-5	0.0	0.0	1.6847551316188614	0.0
6	8.426303549159054E-5	0.0	0.0	2.164296066601503	0.0
7	8.426303549159054E-5	0.0	0.0	2.5453335130944756	0.0
8	8.426303549159054E-5	0.0	0.0	3.1666048737739727	0.0
9	8.426303549159054E-5	0.0	0.0	3.497674340220432	0.0
10	8.426303549159054E-5	0.0	0.0	4.124675587313358	0.0
11	8.426303549159054E-5	0.0	0.0	5.3942667430651525	0.0
12	8.426303549159054E-5	0.0	0.0	6.438959857089892	0.0
13	8.426303549159054E-5	0.0	0.0	6.798763018638984	0.0
14	8.426303549159054E-5	0.0	0.0	6.919596211533924	0.0
15	8.426303549159054E-5	0.0	0.0	7.09048164751087	0.0
16	8.426303549159054E-5	0.0	0.0	7.501348208567865	0.0
17	8.426303549159054E-5	0.0	0.0	8.108294853213792	0.0
18	8.426303549159054E-5	0.0	0.0	8.748862449020864	0.0
19	8.426303549159054E-5	0.0	0.0	9.238346422191514	0.0
20	8.426303549159054E-5	0.0	0.0	9.738953116047053	0.0
21	8.426303549159054E-5	0.0	0.0	10.446425562034447	0.0
22	8.426303549159054E-5	0.0	0.0	11.246250294920625	0.0
23	8.426303549159054E-5	0.0	0.0	12.050962283865314	0.0
24	8.426303549159054E-5	0.0	0.0	12.64678620782635	0.0
25	8.426303549159054E-5	0.0	0.0	13.146044693114025	0.0
26	8.426303549159054E-5	0.0	0.0	13.594155515858303	0.0
27	8.426303549159054E-5	0.0	0.0	14.051956587684115	0.0
28	8.426303549159054E-5	0.0	0.0	14.51843675216556	0.0
29	8.426303549159054E-5	0.0	0.0	15.03876099632613	0.0
30	8.426303549159054E-5	0.0	0.0	15.592706191647848	0.0
31	8.426303549159054E-5	0.0	0.0	16.129545990764772	0.0
32	8.426303549159054E-5	0.0	0.0	16.638326199062995	0.0
33	8.426303549159054E-5	0.0	0.0	17.156965182513733	0.0
34	8.426303549159054E-5	0.0	0.0	17.666672284202367	0.0
35	8.426303549159054E-5	0.0	0.0	18.201068455290034	0.0
36	8.426303549159054E-5	0.0	0.0	18.724089116586335	0.0
37	8.426303549159054E-5	0.0	0.0	19.24719404091813	0.0
38	8.426303549159054E-5	0.0	0.0	19.769709124001484	0.0
39	8.426303549159054E-5	0.0	0.0	20.304863662408575	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	25	3.890883E-5	45.0	11
ATAGCGG	25	3.890883E-5	45.0	2
CGTCGCA	20	7.03338E-4	45.0	37
TACGTCT	25	3.890883E-5	45.0	14
TATTGCG	65	0.0	41.53846	1
CGTTTTT	2645	0.0	40.491493	1
TTATCGG	40	3.4583536E-7	39.375	2
TGTTTCG	350	0.0	39.214287	1
CGGGTAT	155	0.0	39.193546	6
TTCGGGC	455	0.0	39.065933	4
CGGGATA	585	0.0	38.846157	6
GCGGGAT	1160	0.0	38.599136	5
TCTTGCG	315	0.0	38.57143	1
TGCGGGA	2060	0.0	38.228153	4
TTTGGGA	8015	0.0	37.67311	4
TTAATCG	60	1.5643309E-10	37.499996	20
CGTAGAT	60	1.5643309E-10	37.499996	16
CAAGGCG	30	1.1399444E-4	37.499996	42
TTTTGCG	1095	0.0	37.19178	1
CGGGCTC	140	0.0	36.964283	6
>>END_MODULE