##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934386.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1186760 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.735350871279785 34.0 31.0 34.0 31.0 34.0 2 32.939597728268566 34.0 31.0 34.0 31.0 34.0 3 32.9712039502511 34.0 33.0 34.0 31.0 34.0 4 36.33872813374229 37.0 37.0 37.0 35.0 37.0 5 36.292796353095824 37.0 37.0 37.0 35.0 37.0 6 35.98318025548552 37.0 36.0 37.0 35.0 37.0 7 36.22063433213118 37.0 36.0 37.0 35.0 37.0 8 36.31817637938589 37.0 37.0 37.0 35.0 37.0 9 38.14589217701978 39.0 39.0 39.0 37.0 39.0 10 37.819009740806905 39.0 38.0 39.0 35.0 39.0 11 37.706278438774476 39.0 37.0 39.0 35.0 39.0 12 37.60866898109138 39.0 37.0 39.0 35.0 39.0 13 37.62072702147022 39.0 37.0 39.0 35.0 39.0 14 38.98271006774748 40.0 38.0 41.0 35.0 41.0 15 39.02978108463379 40.0 38.0 41.0 35.0 41.0 16 39.045787690855775 40.0 38.0 41.0 35.0 41.0 17 39.007081465502715 40.0 38.0 41.0 35.0 41.0 18 38.934242812363074 40.0 38.0 41.0 35.0 41.0 19 38.93630051568978 40.0 38.0 41.0 35.0 41.0 20 38.8680230206613 40.0 38.0 41.0 35.0 41.0 21 38.77401749300617 40.0 38.0 41.0 35.0 41.0 22 38.78771444942532 40.0 38.0 41.0 35.0 41.0 23 38.72844972867303 40.0 38.0 41.0 35.0 41.0 24 38.68209747547946 40.0 38.0 41.0 35.0 41.0 25 38.63234099565203 40.0 38.0 41.0 35.0 41.0 26 38.600695170042805 40.0 38.0 41.0 35.0 41.0 27 38.56162745626749 40.0 38.0 41.0 35.0 41.0 28 38.47159071758401 40.0 38.0 41.0 35.0 41.0 29 38.41854545148134 40.0 38.0 41.0 35.0 41.0 30 38.37800060669385 40.0 38.0 41.0 35.0 41.0 31 38.285364353365466 40.0 37.0 41.0 34.0 41.0 32 38.17029812261957 40.0 37.0 41.0 34.0 41.0 33 38.06930634669183 40.0 37.0 41.0 34.0 41.0 34 37.93778691563585 40.0 37.0 41.0 34.0 41.0 35 37.866964676935524 40.0 37.0 41.0 34.0 41.0 36 37.73113350635343 40.0 37.0 41.0 33.0 41.0 37 37.69102345882908 40.0 37.0 41.0 33.0 41.0 38 37.65556641612457 40.0 37.0 41.0 33.0 41.0 39 37.59547001921197 40.0 36.0 41.0 33.0 41.0 40 37.50554282247464 40.0 36.0 41.0 33.0 41.0 41 37.42287657150561 40.0 36.0 41.0 33.0 41.0 42 37.32279062320941 40.0 36.0 41.0 33.0 41.0 43 37.26371886480839 40.0 36.0 41.0 33.0 41.0 44 37.14542957295494 40.0 35.0 41.0 33.0 41.0 45 37.13226600155044 39.0 35.0 41.0 33.0 41.0 46 37.051189794061145 39.0 35.0 41.0 32.0 41.0 47 36.920229026930464 39.0 35.0 41.0 32.0 41.0 48 36.8396811486737 39.0 35.0 41.0 32.0 41.0 49 36.800708652128485 39.0 35.0 41.0 32.0 41.0 50 36.707054501331356 39.0 35.0 41.0 32.0 41.0 51 35.82728100037076 38.0 35.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 8.0 9 14.0 10 16.0 11 17.0 12 17.0 13 16.0 14 23.0 15 31.0 16 63.0 17 89.0 18 133.0 19 216.0 20 418.0 21 699.0 22 1116.0 23 1745.0 24 2698.0 25 4529.0 26 6709.0 27 8108.0 28 8606.0 29 8921.0 30 10271.0 31 12701.0 32 17120.0 33 25913.0 34 53512.0 35 72571.0 36 80372.0 37 136378.0 38 278231.0 39 455311.0 40 188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.503421079240958 4.076308604941184 57.72228588762681 16.69798442819104 2 20.476844517846914 4.26202433516465 54.31982877751188 20.94130236947656 3 19.432825508106106 4.070831507634231 54.27146179513971 22.22488118911996 4 18.53247497387846 4.520459065017358 52.04599076477131 24.901075196332872 5 18.205450133135596 5.438757625804712 51.84805689440156 24.507735346658126 6 21.849152313862955 5.961694024065523 54.08599885402272 18.103154808048807 7 85.84246182884492 1.9481613805655735 8.546546900805554 3.6628298897839495 8 88.43692069163099 2.3107452222858877 6.285432606424214 2.9669014796589033 9 82.76247935555631 5.224392463514105 7.946931140247396 4.0661970406821935 10 37.671306751154404 34.17750851056658 14.977333243452762 13.17385149482625 11 25.301830193130876 24.938993562304088 30.88417203141326 18.875004213151776 12 23.780208298223734 22.420118642353973 33.01543698810206 20.784236071320233 13 22.55199029289831 22.631281809295896 33.41223162223196 21.40449627557383 14 20.156139404765916 24.768697967575584 33.39158717853652 21.683575449121978 15 19.1322592605076 26.162408574606495 34.03097509184671 20.6743570730392 16 21.99518015436988 24.877481546395227 32.97911961980519 20.148218679429707 17 22.03798577639961 24.517594121810642 31.00449964609525 22.439920455694494 18 21.271023627355152 25.28910647477165 31.9929893154471 21.4468805824261 19 23.774815463952272 25.876082780006065 30.472968418214297 19.876133337827362 20 25.32373858235869 26.040142910108194 29.805268124978934 18.830850382554182 21 25.3677238868853 25.67890727695574 30.037328524722774 18.91604031143618 22 23.873487478512924 23.7004112036132 30.441032727762984 21.98506859011089 23 22.068404732212073 25.45181839630591 30.36190973743638 22.11786713404564 24 21.860864875796285 24.837709393643195 31.539569921466853 21.761855809093667 25 21.430702079611716 26.37441437190333 29.291095082409246 22.903788466075703 26 20.49369712494523 28.193316256024808 29.44276854629411 21.870218072735852 27 20.443055040614784 27.773602076241193 30.4138157672992 21.36952711584482 28 19.298425966497017 27.072617883986656 32.045148134416394 21.583808015099937 29 20.38145876167043 25.565067916006605 31.803144696484548 22.250328625838417 30 21.79699349489366 25.64275843472985 30.590768141831543 21.969479928544946 31 21.294617277292797 27.88356533755772 28.845090835552263 21.976726549597224 32 22.063686002224543 27.386076376015367 28.492112979877987 22.0581246418821 33 22.51424045299808 27.314284269776536 28.17486265125215 21.99661262597324 34 21.655094543125823 26.525835046681724 28.93314570764097 22.885924702551485 35 21.31492466884627 26.130557147190668 29.250396036266814 23.304122147696248 36 21.255182176682734 28.6833900704439 28.44526273214466 21.616165020728705 37 20.740587818935587 29.16874515487546 28.564663453436246 21.526003572752707 38 21.182631703124475 27.849354545148135 27.624203714314604 23.343810037412787 39 22.019279382520477 26.34559641376521 28.78779197141798 22.847332232296335 40 22.208281371128113 25.845663824193604 28.77624793555563 23.169806869122652 41 20.655819205231047 27.065371262934374 28.942751693687015 23.33605783814756 42 20.85493275809768 27.74520543328053 28.598958508881324 22.80090329974047 43 20.755081061040144 27.320603997438404 29.069230509959894 22.85508443156156 44 20.859651488085206 27.089049175907515 28.557753884525937 23.493545451481342 45 21.67582324985675 25.814992079274663 28.072483063129866 24.436701607738716 46 20.918888402035797 26.813677575920998 29.01285853921602 23.254575482827196 47 20.095217230105497 27.302150392665748 29.7625467659847 22.84008561124406 48 20.240065388115543 26.397081128450573 30.456452863257944 22.90640062017594 49 20.47583336142101 26.291162492837643 29.61609761030031 23.616906535441032 50 20.456789915399913 25.69356904513128 29.20084937139776 24.648791668071052 51 20.299723617243586 25.627338299234893 28.21707843203344 25.855859651488082 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 244.0 1 775.0 2 1306.0 3 5083.0 4 8860.0 5 6017.0 6 3174.0 7 3122.0 8 3070.0 9 3187.5 10 3305.0 11 3309.0 12 3313.0 13 3243.5 14 3174.0 15 3181.0 16 3188.0 17 3213.5 18 3239.0 19 4093.0 20 4947.0 21 5516.5 22 6086.0 23 7125.5 24 8165.0 25 9832.0 26 14012.5 27 16526.0 28 19087.5 29 21649.0 30 24658.0 31 27667.0 32 31418.5 33 35170.0 34 39337.5 35 43505.0 36 46140.5 37 48776.0 38 52222.0 39 55668.0 40 60422.5 41 65177.0 42 68602.0 43 72027.0 44 75399.0 45 78771.0 46 85842.5 47 92914.0 48 97686.5 49 102459.0 50 99313.5 51 96168.0 52 85588.5 53 75009.0 54 67112.5 55 59216.0 56 54895.0 57 50574.0 58 48774.0 59 46974.0 60 43681.0 61 40388.0 62 35493.5 63 30599.0 64 26488.5 65 22378.0 66 18809.0 67 15240.0 68 12555.0 69 9870.0 70 8187.0 71 6504.0 72 5346.5 73 4189.0 74 3485.5 75 2112.0 76 1442.0 77 1115.0 78 788.0 79 630.5 80 473.0 81 291.0 82 109.0 83 74.5 84 40.0 85 58.0 86 76.0 87 56.5 88 37.0 89 30.5 90 24.0 91 12.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1186760.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.669313396619604 #Duplication Level Percentage of deduplicated Percentage of total 1 73.46221496159535 20.326490485822195 2 10.244633514078618 5.669239506691073 3 3.6315744337918647 3.0144951339521557 4 1.7397800300383002 1.9255407556923998 5 1.0530399632766574 1.456844638153332 6 0.7413498468631786 1.2307584749635945 7 0.537357223605475 1.0407813798114098 8 0.4519969303426961 1.000515577596968 9 0.38182554406094504 0.9508365577311371 >10 7.094281328336985 49.810487660682334 >50 0.6098174090525962 10.224300977081292 >100 0.04873581516727283 2.17131937677335 >500 0.0018507271582135524 0.32961107790528993 >1k 0.001542272631844627 0.8487783971434771 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4870 0.410360982844046 No Hit CTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGCT 1408 0.1186423539721595 Illumina Single End Adapter 2 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGC 1319 0.11114294381340793 No Hit TCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGC 1217 0.1025481141932657 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.426303549159054E-5 0.0 0.0 0.1238666621726381 0.0 2 8.426303549159054E-5 0.0 0.0 0.45864370218072736 0.0 3 8.426303549159054E-5 0.0 0.0 0.7495197006976979 0.0 4 8.426303549159054E-5 0.0 0.0 1.1180019549024234 0.0 5 8.426303549159054E-5 0.0 0.0 1.6847551316188614 0.0 6 8.426303549159054E-5 0.0 0.0 2.164296066601503 0.0 7 8.426303549159054E-5 0.0 0.0 2.5453335130944756 0.0 8 8.426303549159054E-5 0.0 0.0 3.1666048737739727 0.0 9 8.426303549159054E-5 0.0 0.0 3.497674340220432 0.0 10 8.426303549159054E-5 0.0 0.0 4.124675587313358 0.0 11 8.426303549159054E-5 0.0 0.0 5.3942667430651525 0.0 12 8.426303549159054E-5 0.0 0.0 6.438959857089892 0.0 13 8.426303549159054E-5 0.0 0.0 6.798763018638984 0.0 14 8.426303549159054E-5 0.0 0.0 6.919596211533924 0.0 15 8.426303549159054E-5 0.0 0.0 7.09048164751087 0.0 16 8.426303549159054E-5 0.0 0.0 7.501348208567865 0.0 17 8.426303549159054E-5 0.0 0.0 8.108294853213792 0.0 18 8.426303549159054E-5 0.0 0.0 8.748862449020864 0.0 19 8.426303549159054E-5 0.0 0.0 9.238346422191514 0.0 20 8.426303549159054E-5 0.0 0.0 9.738953116047053 0.0 21 8.426303549159054E-5 0.0 0.0 10.446425562034447 0.0 22 8.426303549159054E-5 0.0 0.0 11.246250294920625 0.0 23 8.426303549159054E-5 0.0 0.0 12.050962283865314 0.0 24 8.426303549159054E-5 0.0 0.0 12.64678620782635 0.0 25 8.426303549159054E-5 0.0 0.0 13.146044693114025 0.0 26 8.426303549159054E-5 0.0 0.0 13.594155515858303 0.0 27 8.426303549159054E-5 0.0 0.0 14.051956587684115 0.0 28 8.426303549159054E-5 0.0 0.0 14.51843675216556 0.0 29 8.426303549159054E-5 0.0 0.0 15.03876099632613 0.0 30 8.426303549159054E-5 0.0 0.0 15.592706191647848 0.0 31 8.426303549159054E-5 0.0 0.0 16.129545990764772 0.0 32 8.426303549159054E-5 0.0 0.0 16.638326199062995 0.0 33 8.426303549159054E-5 0.0 0.0 17.156965182513733 0.0 34 8.426303549159054E-5 0.0 0.0 17.666672284202367 0.0 35 8.426303549159054E-5 0.0 0.0 18.201068455290034 0.0 36 8.426303549159054E-5 0.0 0.0 18.724089116586335 0.0 37 8.426303549159054E-5 0.0 0.0 19.24719404091813 0.0 38 8.426303549159054E-5 0.0 0.0 19.769709124001484 0.0 39 8.426303549159054E-5 0.0 0.0 20.304863662408575 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAG 25 3.890883E-5 45.0 11 ATAGCGG 25 3.890883E-5 45.0 2 CGTCGCA 20 7.03338E-4 45.0 37 TACGTCT 25 3.890883E-5 45.0 14 TATTGCG 65 0.0 41.53846 1 CGTTTTT 2645 0.0 40.491493 1 TTATCGG 40 3.4583536E-7 39.375 2 TGTTTCG 350 0.0 39.214287 1 CGGGTAT 155 0.0 39.193546 6 TTCGGGC 455 0.0 39.065933 4 CGGGATA 585 0.0 38.846157 6 GCGGGAT 1160 0.0 38.599136 5 TCTTGCG 315 0.0 38.57143 1 TGCGGGA 2060 0.0 38.228153 4 TTTGGGA 8015 0.0 37.67311 4 TTAATCG 60 1.5643309E-10 37.499996 20 CGTAGAT 60 1.5643309E-10 37.499996 16 CAAGGCG 30 1.1399444E-4 37.499996 42 TTTTGCG 1095 0.0 37.19178 1 CGGGCTC 140 0.0 36.964283 6 >>END_MODULE