##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934385.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1660440
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.071941774469416	33.0	31.0	34.0	30.0	34.0
2	32.276249668762496	34.0	31.0	34.0	30.0	34.0
3	32.31005095035051	34.0	31.0	34.0	30.0	34.0
4	35.88359410758594	37.0	35.0	37.0	35.0	37.0
5	35.874206234492064	37.0	35.0	37.0	35.0	37.0
6	35.5554702368047	37.0	35.0	37.0	33.0	37.0
7	35.918674568186745	37.0	35.0	37.0	35.0	37.0
8	36.00381404928814	37.0	35.0	37.0	35.0	37.0
9	37.81085495410855	39.0	38.0	39.0	35.0	39.0
10	37.18975090939751	39.0	37.0	39.0	34.0	39.0
11	37.06741345667413	39.0	37.0	39.0	33.0	39.0
12	36.99934414974344	39.0	37.0	39.0	33.0	39.0
13	37.00543711305437	39.0	37.0	39.0	33.0	39.0
14	38.175671508756714	40.0	38.0	41.0	33.0	41.0
15	38.18837838163378	40.0	37.0	41.0	33.0	41.0
16	38.195104309701044	40.0	37.0	41.0	33.0	41.0
17	38.146699669967	40.0	37.0	41.0	33.0	41.0
18	38.05415552504156	39.0	37.0	41.0	33.0	41.0
19	38.036265086362654	40.0	37.0	41.0	34.0	41.0
20	37.916165594661656	40.0	36.0	41.0	34.0	41.0
21	37.77624244176243	39.0	36.0	41.0	33.0	41.0
22	37.87266387222664	39.0	36.0	41.0	33.0	41.0
23	37.79099576015996	39.0	36.0	41.0	33.0	41.0
24	37.74910626099106	39.0	36.0	41.0	33.0	41.0
25	37.655918310809184	39.0	36.0	41.0	33.0	41.0
26	37.571043217460435	39.0	36.0	41.0	33.0	41.0
27	37.49203343692034	39.0	36.0	41.0	33.0	41.0
28	37.36208595312086	39.0	36.0	41.0	32.0	41.0
29	37.30024511575245	39.0	36.0	41.0	32.0	41.0
30	37.26795427717954	39.0	36.0	41.0	32.0	41.0
31	37.15783828382838	39.0	35.0	41.0	32.0	41.0
32	36.91632519091325	39.0	35.0	41.0	31.0	41.0
33	36.70592794680928	39.0	35.0	41.0	30.0	41.0
34	36.462716508877165	39.0	35.0	41.0	30.0	41.0
35	36.32274216472742	39.0	35.0	41.0	30.0	41.0
36	36.130123340801234	39.0	35.0	40.0	29.0	41.0
37	36.03637168461372	39.0	35.0	40.0	29.0	41.0
38	35.94746814097468	39.0	35.0	40.0	28.0	41.0
39	35.86461660764616	39.0	35.0	40.0	28.0	41.0
40	35.7766899135169	39.0	35.0	40.0	27.0	41.0
41	35.72543843800438	39.0	35.0	40.0	27.0	41.0
42	35.595371708703716	39.0	35.0	40.0	26.0	41.0
43	35.442859121678595	38.0	35.0	40.0	26.0	41.0
44	35.27547698200477	38.0	34.0	40.0	25.0	41.0
45	35.3465394714654	38.0	34.0	40.0	26.0	41.0
46	35.25170798101708	38.0	34.0	40.0	25.0	41.0
47	35.008631447086316	38.0	34.0	40.0	24.0	41.0
48	34.8952898027029	38.0	34.0	40.0	24.0	41.0
49	34.874149622991496	38.0	34.0	40.0	24.0	41.0
50	34.76615174291152	38.0	34.0	40.0	24.0	41.0
51	33.7694297896943	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	9.0
11	18.0
12	14.0
13	16.0
14	13.0
15	34.0
16	50.0
17	155.0
18	328.0
19	634.0
20	1256.0
21	2223.0
22	3570.0
23	5788.0
24	9448.0
25	15754.0
26	21989.0
27	23985.0
28	24359.0
29	26064.0
30	30927.0
31	37994.0
32	48299.0
33	63915.0
34	94435.0
35	120619.0
36	148022.0
37	220554.0
38	365660.0
39	394178.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.362337693623378	4.193165666931656	56.2153405121534	15.229156127291562
2	23.026125605261257	4.49838596998386	53.50136108501361	18.974127339741273
3	18.77785406277854	4.458035219580352	56.346088988460885	20.418021729180218
4	16.628664691286644	4.027667365276674	56.84071691840716	22.50295102502951
5	16.5899400158994	5.14399797643998	56.2926694129267	21.973392594733927
6	19.67478499674785	6.159933511599335	57.39605164896051	16.769229842692297
7	82.21001662210017	1.1627038616270386	12.707896702078965	3.919382814193828
8	84.8927392739274	1.8703476187034762	9.739346197393461	3.497566909975669
9	80.15411577654116	4.33752499337525	11.246175712461758	4.262183517621835
10	34.471826744718264	34.74410397244104	18.379164558791647	12.404904724049047
11	22.705126352051263	26.629869191298695	31.316518513165185	19.348485943484857
12	20.257642552576424	23.697634361976345	35.44253330442533	20.6021897810219
13	21.27809496278095	23.811459613114597	36.54368721543687	18.366758208667584
14	20.450001204500012	26.95634891956349	33.85530341355303	18.738346462383465
15	18.300450483004504	26.53429211534292	35.22578352725784	19.93947387439474
16	20.184890751848908	27.71446122714461	32.11582472115825	19.984823299848234
17	21.21865288718653	25.932885259328852	31.567114740671148	21.28134711281347
18	20.811893233118933	25.51022620510226	33.33815133338152	20.339729228397292
19	20.617547156175473	27.341608248416083	32.048312495483124	19.99253209992532
20	22.422490424224904	27.85165377851654	31.556635590566355	18.169220206692202
21	22.25139119751391	27.856592228565923	32.1869504468695	17.70506612705066
22	21.38481366384814	24.558791645587917	31.909554094095544	22.146840596468405
23	19.729589747295897	27.186589096865895	30.945833634458335	22.137987521379877
24	20.93999180939992	26.732793717327937	30.941136084411358	21.386078388860785
25	19.582821420828214	29.432439594324393	29.214304642143045	21.77043434270434
26	19.720194647201946	29.473934619739346	30.063597600635976	20.74227313242273
27	20.06763267567633	28.383139408831394	30.97329623473296	20.575931680759314
28	17.526499000264987	28.809893763098938	32.3366697983667	21.326937438269375
29	18.65601888656019	26.875888318758882	32.52872732528727	21.939365469393653
30	20.49438702994387	27.60045530100455	31.80301606803016	20.102141601021415
31	20.682951506829514	29.48019801980198	29.015863265158632	20.82098720820987
32	20.005781600057816	30.98401628484016	28.031184505311845	20.979017609790176
33	20.918310809183108	28.540025535400254	28.503288285032884	22.038375370383754
34	19.578063645780635	28.619642986196432	28.39614800896148	23.406145359061455
35	20.468851629688515	29.01797114017971	27.567572450675726	22.945604779456048
36	22.256028522560285	29.748801522488016	27.384307773843076	20.610862181108622
37	21.072125460721256	30.57039098070391	27.996073329960737	20.3614102286141
38	20.277938377779385	31.305437113054367	27.668268651682688	20.74835585748356
39	19.93754667437547	28.690106236901062	29.1006600660066	22.271687022716872
40	21.394871238948713	26.28339476283395	29.276095492760955	23.045638505456385
41	22.451579099515794	26.563802365638022	26.58000289080003	24.404615644046157
42	24.126617041266172	27.075473970754736	26.446363614463635	22.351545373515457
43	22.709402327094022	27.43766712437667	27.665799426657994	22.18713112187131
44	20.331177278311774	27.895316903953173	27.32625087326251	24.447254944472547
45	20.895726433957265	27.40665124906651	27.011996820119965	24.685625496856257
46	21.937498494374985	27.70392185203922	27.858579653585796	22.5
47	19.897557273975572	28.560622485606224	28.97286261472863	22.568957625689574
48	21.285562862855627	28.16861795668618	28.630242586302423	21.915576594155766
49	22.01199682011997	26.378550263785506	29.57288429572884	22.03656862036569
50	21.427814314278145	25.813699983137	28.046903230469034	24.711582472115825
51	20.646816506468166	24.865999373659996	26.903531594035318	27.583652525836527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	155.0
1	497.0
2	839.0
3	9178.5
4	17518.0
5	12109.0
6	6700.0
7	6976.0
8	7252.0
9	7631.0
10	8010.0
11	8670.0
12	9330.0
13	9460.5
14	9591.0
15	9510.5
16	9430.0
17	9220.5
18	9011.0
19	8869.5
20	8728.0
21	8484.0
22	8240.0
23	9035.0
24	9830.0
25	10902.0
26	13846.0
27	15718.0
28	17759.5
29	19801.0
30	23899.0
31	27997.0
32	32379.0
33	36761.0
34	41836.0
35	46911.0
36	52604.5
37	58298.0
38	66109.5
39	73921.0
40	84812.5
41	95704.0
42	105597.5
43	115491.0
44	128338.5
45	141186.0
46	162186.5
47	183187.0
48	192800.0
49	202413.0
50	189046.5
51	175680.0
52	146892.5
53	118105.0
54	98141.5
55	78178.0
56	65619.5
57	53061.0
58	45723.5
59	38386.0
60	31920.0
61	25454.0
62	20954.0
63	16454.0
64	13010.0
65	9566.0
66	7444.5
67	5323.0
68	3904.0
69	2485.0
70	2010.5
71	1536.0
72	1227.0
73	918.0
74	805.0
75	526.0
76	360.0
77	249.5
78	139.0
79	97.0
80	55.0
81	43.5
82	32.0
83	20.5
84	9.0
85	8.0
86	7.0
87	5.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1660440.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.9422547947425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.65993634414858	26.115619454511595
2	12.064094596022635	8.672215236762433
3	4.696793655734463	5.064400628782145
4	2.3018472587393166	3.309343226887523
5	1.3862202586949635	2.491194086982412
6	0.9156645695579335	1.974662955934168
7	0.6596057686253703	1.6595403020010522
8	0.5033151479201787	1.447222503088046
9	0.40866795982227155	1.321960314454171
>10	3.9809999447109226	31.2413456458492
>50	0.3446140168831697	7.9456767609251955
>100	0.07149563994130811	4.563901376576068
>500	0.00421552449932682	1.0666082015816594
>1k	0.002023451759676874	1.6729730550860915
>5k	3.3724195994614564E-4	0.7749106463675832
>10k+	1.6862097997307282E-4	0.678425604210756
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11194	0.6741586567415866	No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT	7663	0.46150417961504175	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	5123	0.30853267808532675	No Hit
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	4594	0.2766736527667365	No Hit
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	4319	0.26011177760111776	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCC	3225	0.19422562694225626	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG	2263	0.13628917636289176	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC	2183	0.13147117631471175	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC	1964	0.11828190118281902	No Hit
CGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG	1853	0.11159692611596927	No Hit
TCCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG	1808	0.10888680108886802	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCT	1735	0.10449037604490376	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0112500301125E-4	0.0	0.0	0.5120931801209317	0.0
2	3.0112500301125E-4	0.0	0.0	1.4811736648117366	0.0
3	3.0112500301125E-4	0.0	0.0	2.0648141456481413	0.0
4	3.0112500301125E-4	0.0	0.0	2.8698417286984172	0.0
5	3.0112500301125E-4	0.0	0.0	4.204307292043073	0.0
6	3.0112500301125E-4	0.0	0.0	5.059923875599239	0.0
7	3.0112500301125E-4	0.0	0.0	5.80942400809424	0.0
8	3.0112500301125E-4	0.0	0.0	6.845534918455349	0.0
9	3.0112500301125E-4	0.0	0.0	7.3432945484329455	0.0
10	3.0112500301125E-4	0.0	0.0	8.582363710823637	0.0
11	3.0112500301125E-4	0.0	0.0	10.82682903326829	0.0
12	3.0112500301125E-4	0.0	0.0	12.964455204644553	0.0
13	3.0112500301125E-4	0.0	0.0	13.671195586711956	0.0
14	3.0112500301125E-4	0.0	0.0	13.92480306424803	0.0
15	3.0112500301125E-4	0.0	0.0	14.241827467418275	0.0
16	3.0112500301125E-4	0.0	0.0	14.960070824600708	0.0
17	3.0112500301125E-4	0.0	0.0	16.12283491122835	0.0
18	3.0112500301125E-4	0.0	0.0	17.382139673821396	0.0
19	3.0112500301125E-4	0.0	0.0	18.37235913372359	0.0
20	3.0112500301125E-4	0.0	0.0	19.28079304280793	0.0
21	3.0112500301125E-4	0.0	0.0	20.593577605935778	0.0
22	3.0112500301125E-4	0.0	0.0	22.050540820505407	0.0
23	3.0112500301125E-4	0.0	0.0	23.549420635494208	0.0
24	3.0112500301125E-4	0.0	0.0	24.588000770880008	0.0
25	3.0112500301125E-4	0.0	0.0	25.46740622967406	0.0
26	3.0112500301125E-4	0.0	0.0	26.28724916287249	0.0
27	3.0112500301125E-4	0.0	0.0	27.069150345691504	0.0
28	3.0112500301125E-4	0.0	0.0	27.956144154561443	0.0
29	3.0112500301125E-4	0.0	0.0	28.846811688468115	0.0
30	3.6135000361350004E-4	0.0	0.0	29.833899448338993	0.0
31	3.6135000361350004E-4	0.0	0.0	30.71709908217099	0.0
32	3.6135000361350004E-4	0.0	0.0	31.544108790441086	0.0
33	3.6135000361350004E-4	0.0	0.0	32.38057382380574	0.0
34	3.6135000361350004E-4	0.0	0.0	33.19132278191323	0.0
35	3.6135000361350004E-4	0.0	0.0	34.07151116571511	0.0
36	3.6135000361350004E-4	0.0	0.0	34.914781624147814	0.0
37	3.6135000361350004E-4	0.0	0.0	35.75648623256486	0.0
38	3.6135000361350004E-4	0.0	0.0	36.60891089108911	0.0
39	3.6135000361350004E-4	0.0	0.0	37.463684324636844	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGCG	20	7.034193E-4	45.000004	15
TAACGGT	30	2.1660871E-6	45.000004	24
CGCTAAG	20	7.034193E-4	45.000004	16
GTCCGAC	30	2.1660871E-6	45.000004	19
ACTCGCG	20	7.034193E-4	45.000004	1
CCGGTAT	20	7.034193E-4	45.000004	22
TCGACGG	20	7.034193E-4	45.000004	2
CGATAAG	20	7.034193E-4	45.000004	10
GTACGAC	20	7.034193E-4	45.000004	9
TCCGGTA	20	7.034193E-4	45.000004	21
GACGTTG	35	1.2123564E-7	45.0	1
CGACCGG	25	3.8915576E-5	45.0	18
CATACGT	25	3.8915576E-5	45.0	19
ACGTCCC	35	1.2123564E-7	45.0	21
TATAGCG	35	1.2123564E-7	45.0	38
TTCGACC	25	3.8915576E-5	45.0	29
CGTTTTT	8590	0.0	43.559372	1
CCGTCGG	80	0.0	42.187504	2
GCCGATC	60	3.6379788E-12	41.250004	9
CGAATAT	60	3.6379788E-12	41.250004	14
>>END_MODULE