##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934384.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174513
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14477319535842	33.0	31.0	34.0	30.0	34.0
2	32.33115512557119	34.0	31.0	34.0	30.0	34.0
3	32.401719691480636	34.0	31.0	34.0	30.0	34.0
4	35.91190646676537	37.0	35.0	37.0	35.0	37.0
5	35.88083316234048	37.0	35.0	37.0	35.0	37.0
6	35.55084618050205	37.0	35.0	37.0	33.0	37.0
7	35.88774155756471	37.0	35.0	37.0	35.0	37.0
8	35.9824701812581	37.0	35.0	37.0	35.0	37.0
9	37.76373782154816	39.0	38.0	39.0	35.0	39.0
10	37.2337760416445	39.0	37.0	39.0	34.0	39.0
11	37.11376119293699	39.0	37.0	39.0	33.0	39.0
12	36.933482217736206	39.0	37.0	39.0	33.0	39.0
13	36.8542068074172	39.0	37.0	39.0	33.0	39.0
14	37.962760735726214	40.0	37.0	41.0	33.0	41.0
15	38.00622726185236	40.0	37.0	41.0	33.0	41.0
16	38.04986917982176	40.0	37.0	41.0	33.0	41.0
17	38.000194123010985	40.0	37.0	41.0	33.0	41.0
18	37.9701825352295	40.0	37.0	41.0	33.0	41.0
19	38.02147358096504	40.0	37.0	41.0	33.0	41.0
20	37.964946322433214	40.0	37.0	41.0	33.0	41.0
21	37.821830835418595	40.0	36.0	41.0	33.0	41.0
22	37.907045728740336	40.0	36.0	41.0	33.0	41.0
23	37.86478736293255	40.0	36.0	41.0	33.0	41.0
24	37.8157219204896	40.0	36.0	41.0	33.0	41.0
25	37.75516235239627	40.0	36.0	41.0	33.0	41.0
26	37.69140912020556	40.0	36.0	41.0	33.0	41.0
27	37.6115275011856	39.0	36.0	41.0	33.0	41.0
28	37.447022723460705	39.0	36.0	41.0	32.0	41.0
29	37.37013298277669	39.0	36.0	41.0	32.0	41.0
30	37.349533806777785	39.0	36.0	41.0	32.0	41.0
31	37.252759228718624	39.0	35.0	41.0	32.0	41.0
32	37.11493018808647	39.0	35.0	41.0	31.0	41.0
33	37.072928098709845	39.0	35.0	41.0	31.0	41.0
34	36.92951291301161	39.0	35.0	41.0	31.0	41.0
35	36.870153842486204	39.0	35.0	41.0	31.0	41.0
36	36.71368388429928	39.0	35.0	41.0	31.0	41.0
37	36.605058436986226	39.0	35.0	41.0	30.0	41.0
38	36.50180372631039	39.0	35.0	40.0	30.0	41.0
39	36.49566586321309	39.0	35.0	40.0	30.0	41.0
40	36.4582086362603	39.0	35.0	40.0	30.0	41.0
41	36.361599233043826	39.0	35.0	40.0	30.0	41.0
42	36.260825550675044	38.0	35.0	40.0	30.0	41.0
43	36.171101554431495	38.0	35.0	40.0	30.0	41.0
44	36.01777247250563	38.0	35.0	40.0	29.0	41.0
45	36.07658919058367	38.0	35.0	40.0	29.0	41.0
46	35.95108781256572	38.0	35.0	40.0	29.0	41.0
47	35.68027173815871	38.0	34.0	40.0	28.0	41.0
48	35.564382003434616	38.0	34.0	40.0	28.0	41.0
49	35.575634326737976	38.0	34.0	40.0	28.0	41.0
50	35.47073297613564	38.0	34.0	40.0	28.0	41.0
51	34.4975304658186	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	13.0
10	22.0
11	19.0
12	22.0
13	19.0
14	22.0
15	33.0
16	59.0
17	96.0
18	175.0
19	399.0
20	742.0
21	1266.0
22	1942.0
23	2920.0
24	4246.0
25	6463.0
26	8678.0
27	11049.0
28	13311.0
29	16380.0
30	20684.0
31	26456.0
32	34368.0
33	47740.0
34	79150.0
35	92791.0
36	102261.0
37	154056.0
38	253058.0
39	295960.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.678920539832255	4.676065739587386	54.256359870005696	17.388653850574663
2	20.1266397221657	4.217748121987581	54.071772726227806	21.583839429618916
3	20.560691963392486	4.140354342608383	53.59489422424443	21.7040594697547
4	19.09114671357405	4.9297879206105	51.556943175597034	24.422122190218413
5	17.734329036800784	5.816708712462101	51.25188056666891	25.197081684068205
6	21.42811531247419	6.0502523173434435	53.70608924720288	18.81554312297948
7	83.86514240370263	2.0899726099242835	9.378525397334895	4.66635958903818
8	85.80501024680017	2.344375924319271	7.238744909592317	4.61186891928825
9	80.36454258062703	5.08065896248062	9.355196579348206	5.19960187754414
10	39.21557275228116	30.933501800320645	16.09194619386929	13.75897925352891
11	26.465522305840807	25.188908083605714	27.85367211772028	20.4918974928332
12	24.845020872480763	22.172934654618555	32.774520162824935	20.20752431007575
13	23.655932288531503	22.97599090005815	33.692432523096805	19.67564428831354
14	20.435959414668037	25.154595989997556	32.559622583998646	21.84982201133576
15	18.15646144401978	24.636764344030247	35.603607622904136	21.603166589045845
16	22.14628531144398	24.203733802861272	32.414285750775	21.235695134919748
17	22.103714475701842	23.84511708256954	30.11512005401388	23.936048387714738
18	21.848885452949435	24.347282660983744	32.26162673380371	21.542205152263108
19	23.23822724822969	25.137056805671797	30.662836426672165	20.96187951942635
20	24.431402632410197	26.388809659833477	29.850244314026327	19.32954339373
21	24.405519564278983	26.25207213542975	30.800851076148156	18.541557224143112
22	22.744746120306885	23.916636086616325	30.704640987370937	22.633976805705856
23	21.471537564931168	25.256425429092737	29.603418608393433	23.66861839758266
24	22.571142252150466	24.871925640669794	30.017888265178843	22.539043842000897
25	21.669917659489506	25.571790180270458	28.053414479022372	24.704877681217663
26	21.15489568868118	27.585220427530388	27.975424707942782	23.284459175845647
27	21.385203910046123	25.778854725320194	30.599746448102323	22.236194916531364
28	19.1596006174474	25.836495636915046	32.18167870427999	22.822225041357566
29	21.326456156721978	25.20193475934281	30.50362150099658	22.967987582938633
30	24.590362133071324	24.7950427113195	29.61031508378366	21.004280071825516
31	24.135705607345344	27.006938194809255	27.55695339259761	21.300402805247792
32	23.776578037024706	28.63220756177241	26.3010285965332	21.290185804669683
33	24.80815452872808	27.05615008092716	26.59383080476759	21.541864585577173
34	22.369526774075723	26.022530189108167	28.675970380915324	22.931972655900786
35	22.444110878295938	27.542223883430832	27.1073202254892	22.906345012784023
36	25.255829437392347	27.343247797172104	26.97730889313273	20.423613872302816
37	23.07194556382092	27.907651937441308	28.83646243166317	20.18394006707461
38	21.366217317305132	29.239182537783748	27.722894510320444	21.671705634590676
39	23.14099545939466	27.273431626555006	27.864399968327298	21.721172945723037
40	25.308446990369625	25.13807850572961	27.280157818602262	22.273316685298504
41	22.298348336714877	26.66202928362649	26.789145799152497	24.250476580506135
42	21.853823669895522	27.265258026092514	27.079649182256816	23.801269121755144
43	21.986985244096914	26.026020997639023	28.586571625856845	23.40042213240722
44	22.42614598561276	24.607135042353725	28.427101275166816	24.5396176968667
45	23.96984963129399	24.06282433655481	26.541383535133285	25.42594249701791
46	24.72999447430552	24.61360580938653	28.300240184655255	22.356159531652693
47	21.369708125835984	26.212140691503627	30.32686739099525	22.09128379166514
48	21.139825612828467	26.171272689191177	30.11256580386935	22.576335894111004
49	23.138866917607555	24.40568984762195	30.489317700187225	21.96612553458327
50	23.078246047510756	23.588585226387448	28.56196568279789	24.771203043303906
51	21.720747237365615	24.1348541906305	27.331838813193215	26.81255975881067
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	207.0
1	741.5
2	1276.0
3	3376.5
4	5477.0
5	3755.0
6	2033.0
7	2052.5
8	2072.0
9	2239.0
10	2406.0
11	2636.5
12	2867.0
13	2986.5
14	3106.0
15	3129.5
16	3153.0
17	3284.5
18	3416.0
19	3351.0
20	3286.0
21	3866.0
22	4446.0
23	5229.0
24	6012.0
25	6689.0
26	9122.0
27	10878.0
28	12696.5
29	14515.0
30	17723.0
31	20931.0
32	23901.0
33	26871.0
34	30898.5
35	34926.0
36	36918.5
37	38911.0
38	43107.5
39	47304.0
40	51923.0
41	56542.0
42	62237.5
43	67933.0
44	75513.5
45	83094.0
46	92556.0
47	102018.0
48	115814.5
49	129611.0
50	126758.0
51	123905.0
52	107743.5
53	91582.0
54	78349.5
55	65117.0
56	58280.0
57	51443.0
58	46924.5
59	42406.0
60	39412.0
61	36418.0
62	32271.0
63	28124.0
64	24214.0
65	20304.0
66	16401.5
67	12499.0
68	10308.0
69	8117.0
70	6865.5
71	5614.0
72	4562.5
73	3511.0
74	2844.5
75	1598.0
76	1018.0
77	882.0
78	746.0
79	534.0
80	322.0
81	332.0
82	342.0
83	226.0
84	110.0
85	86.5
86	63.0
87	44.0
88	25.0
89	15.0
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1174513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.20879613476044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.24556477703156	19.81542816166392
2	12.367397206336737	6.977387730223176
3	5.088205943877439	4.305964924875449
4	2.604925315712253	2.9392722870881363
5	1.4804163875646572	2.0880382035684955
6	1.0062923517660785	1.7031777481762762
7	0.67655654069601	1.3359391871092747
8	0.4840702630881584	1.0924031493082969
9	0.3608803250230571	0.9161999565859353
>10	4.528746616539753	32.61418376635422
>50	1.0695648633176738	19.348163579989368
>100	0.0818991673643326	4.137886312312171
>500	0.003044578712335978	0.6134686992180093
>1k	0.0021312050986351844	1.6522344722884028
>5k	3.044578712335978E-4	0.46025182123887887
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	5359	0.45627421748418284	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	4736	0.40323095614948495	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	3820	0.3252411850698971	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	3787	0.3224315099109163	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3166	0.26955853191918694	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	1534	0.13060732405686443	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC	1176	0.10012660566549711	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.514167148426624E-5	0.0	0.0	0.441629849988889	0.0
2	8.514167148426624E-5	0.0	0.0	1.69934262115447	0.0
3	8.514167148426624E-5	0.0	0.0	2.356210616655584	0.0
4	8.514167148426624E-5	0.0	0.0	3.0914940915937072	0.0
5	8.514167148426624E-5	0.0	0.0	4.236053581356699	0.0
6	8.514167148426624E-5	0.0	0.0	4.922380595191369	0.0
7	8.514167148426624E-5	0.0	0.0	5.584101665967086	0.0
8	8.514167148426624E-5	0.0	0.0	6.618572974500921	0.0
9	8.514167148426624E-5	0.0	0.0	7.099538276715541	0.0
10	8.514167148426624E-5	0.0	0.0	8.071685881722892	0.0
11	8.514167148426624E-5	0.0	0.0	10.09686567964765	0.0
12	1.7028334296853248E-4	0.0	0.0	11.856573745884464	0.0
13	1.7028334296853248E-4	0.0	0.0	12.43902792050833	0.0
14	1.7028334296853248E-4	0.0	0.0	12.64081368192604	0.0
15	1.7028334296853248E-4	0.0	0.0	12.958051549876417	0.0
16	1.7028334296853248E-4	0.0	0.0	13.63765237166383	0.0
17	1.7028334296853248E-4	0.0	0.0	14.663098663020333	0.0
18	1.7028334296853248E-4	0.0	0.0	15.777007151048988	0.0
19	1.7028334296853248E-4	0.0	0.0	16.573252062769846	0.0
20	1.7028334296853248E-4	0.0	0.0	17.40874728504495	0.0
21	1.7028334296853248E-4	0.0	0.0	18.50239205526035	0.0
22	1.7028334296853248E-4	0.0	0.0	19.645078428250688	0.0
23	1.7028334296853248E-4	0.0	0.0	20.707901913388785	0.0
24	1.7028334296853248E-4	0.0	0.0	21.505849658539326	0.0
25	2.5542501445279875E-4	0.0	0.0	22.197966306034928	0.0
26	2.5542501445279875E-4	0.0	0.0	22.837124833867314	0.0
27	2.5542501445279875E-4	0.0	0.0	23.47543194498486	0.0
28	3.4056668593706495E-4	0.0	0.0	24.158523575303125	0.0
29	3.4056668593706495E-4	0.0	0.0	24.863241190178396	0.0
30	3.4056668593706495E-4	0.0	0.0	25.65420731826723	0.0
31	3.4056668593706495E-4	0.0	0.0	26.34887821590736	0.0
32	4.257083574213312E-4	0.0	0.0	27.011365561726436	0.0
33	4.257083574213312E-4	0.0	0.0	27.670958090715047	0.0
34	4.257083574213312E-4	0.0	0.0	28.35992449636573	0.0
35	4.257083574213312E-4	0.0	0.0	29.071623728302708	0.0
36	4.257083574213312E-4	0.0	0.0	29.727384882074528	0.0
37	4.257083574213312E-4	0.0	0.0	30.385359719304937	0.0
38	4.257083574213312E-4	0.0	0.0	31.10208230985949	0.0
39	4.257083574213312E-4	0.0	0.0	31.883682854085055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGT	30	2.1655433E-6	45.000004	42
GTCCGCG	25	3.8908576E-5	45.000004	1
GTAAGCG	25	3.8908576E-5	45.000004	19
CGATCTA	30	2.1655433E-6	45.000004	5
TTTAGCG	70	0.0	45.0	1
CGAACTT	20	7.03335E-4	45.0	20
GACCGTA	20	7.03335E-4	45.0	22
GCACGTC	20	7.03335E-4	45.0	45
TAGTTCG	20	7.03335E-4	45.0	24
AGCGCGA	20	7.03335E-4	45.0	41
TGTCGAT	20	7.03335E-4	45.0	37
TACCGGT	20	7.03335E-4	45.0	40
TCTAGCG	75	0.0	42.0	1
TTATCCG	60	3.6379788E-12	41.250004	1
TCCGAAC	55	6.184564E-11	40.909092	17
CGAATAT	95	0.0	40.26316	14
TCGATTG	45	1.9283107E-8	40.000004	1
TTGAGCG	45	1.9283107E-8	40.000004	1
AGTTGCG	45	1.9283107E-8	40.000004	1
CTTTACG	45	1.9283107E-8	40.000004	1
>>END_MODULE