##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934382.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2488051
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18026640129161	33.0	31.0	34.0	30.0	34.0
2	32.389967086687534	34.0	31.0	34.0	30.0	34.0
3	32.421652932355485	34.0	31.0	34.0	30.0	34.0
4	35.963864084779615	37.0	35.0	37.0	35.0	37.0
5	35.93079120966572	37.0	35.0	37.0	35.0	37.0
6	35.53457505493255	37.0	35.0	37.0	33.0	37.0
7	35.88322345482468	37.0	35.0	37.0	35.0	37.0
8	35.94201405035508	37.0	35.0	37.0	35.0	37.0
9	37.75996432549011	39.0	38.0	39.0	35.0	39.0
10	37.29167569314295	39.0	37.0	39.0	34.0	39.0
11	37.17367650421957	39.0	37.0	39.0	34.0	39.0
12	36.93630034111037	39.0	37.0	39.0	33.0	39.0
13	36.833480503414116	39.0	37.0	39.0	33.0	39.0
14	37.848377706084	40.0	37.0	41.0	33.0	41.0
15	37.918018963437646	40.0	37.0	41.0	33.0	41.0
16	38.02648257612083	40.0	37.0	41.0	33.0	41.0
17	37.96350717891233	40.0	37.0	41.0	33.0	41.0
18	37.875895228835745	39.0	37.0	41.0	33.0	41.0
19	37.824566698994516	39.0	37.0	41.0	33.0	41.0
20	37.66193377868862	39.0	35.0	41.0	33.0	41.0
21	37.53653924296568	39.0	35.0	41.0	33.0	41.0
22	37.64318094765742	39.0	35.0	41.0	33.0	41.0
23	37.59654122845553	39.0	35.0	41.0	33.0	41.0
24	37.522597808485436	39.0	35.0	41.0	33.0	41.0
25	37.4456532442462	39.0	35.0	41.0	33.0	41.0
26	37.38319994244491	39.0	35.0	41.0	33.0	41.0
27	37.2957965893786	39.0	35.0	41.0	33.0	41.0
28	37.1679189855835	39.0	35.0	41.0	32.0	41.0
29	37.040682847739056	39.0	35.0	41.0	32.0	41.0
30	36.96877154045476	39.0	35.0	41.0	32.0	41.0
31	36.84897938185351	39.0	35.0	41.0	31.0	41.0
32	36.62354991919378	39.0	35.0	41.0	31.0	41.0
33	36.44350698599024	39.0	35.0	41.0	30.0	41.0
34	36.1251791060553	39.0	35.0	41.0	30.0	41.0
35	35.97805149492515	39.0	35.0	41.0	29.0	41.0
36	35.789088728486675	38.0	35.0	40.0	28.0	41.0
37	35.68918563164501	38.0	35.0	40.0	28.0	41.0
38	35.61942460182689	38.0	35.0	40.0	27.0	41.0
39	35.5439542838953	38.0	35.0	40.0	27.0	41.0
40	35.39138626981521	38.0	35.0	40.0	26.0	41.0
41	35.26778751721729	38.0	34.0	40.0	26.0	41.0
42	35.17833034773001	38.0	34.0	40.0	26.0	41.0
43	35.08302000240349	38.0	34.0	40.0	25.0	41.0
44	34.94475153443398	38.0	34.0	40.0	25.0	41.0
45	35.01042341977717	38.0	34.0	40.0	25.0	41.0
46	34.97122044524007	38.0	34.0	40.0	25.0	41.0
47	34.7642246883203	37.0	34.0	40.0	24.0	41.0
48	34.67275590411933	37.0	34.0	40.0	24.0	41.0
49	34.681427350162835	37.0	34.0	40.0	24.0	41.0
50	34.55751952029922	37.0	34.0	40.0	24.0	41.0
51	33.6308556376055	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	31.0
10	36.0
11	47.0
12	38.0
13	46.0
14	45.0
15	80.0
16	164.0
17	349.0
18	678.0
19	1394.0
20	2550.0
21	4329.0
22	6666.0
23	9811.0
24	14648.0
25	23496.0
26	32950.0
27	38642.0
28	40170.0
29	43042.0
30	49706.0
31	60777.0
32	77718.0
33	108818.0
34	175316.0
35	193208.0
36	215565.0
37	310629.0
38	492709.0
39	584214.0
40	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.114357382545613	4.240266779097373	51.991337798139995	20.65403804021702
2	22.02221739023838	5.9203368419698785	50.99047406986432	21.066971697927414
3	18.81967049710798	6.130983649450916	54.39836241298912	20.650983440451984
4	17.739949864371752	4.659148867929154	54.0912545602964	23.509646707402702
5	18.78739623906423	5.507202223748629	52.29370298277648	23.411698554410663
6	20.543992064471347	7.299287675373215	54.47553124915848	17.681189010996963
7	79.84567036608172	2.074676121992676	13.732274780541074	4.347378731384525
8	81.9252901166415	3.5523387583293107	10.86456829060176	3.6578028344274296
9	77.16702752475733	4.1567073986827445	12.568713422675017	6.107551653884908
10	41.013829700436204	21.88608674018338	21.044705273324382	16.055378286056033
11	29.83994299152228	22.8849006712483	27.684360167858298	19.590796169371128
12	26.515493452505595	20.28784779733213	31.68821700198268	21.5084417481796
13	25.611010385237282	18.8063669112892	34.49764494377326	21.084977759700262
14	21.789706079176028	21.65156582401245	34.92753966860004	21.63118842821148
15	19.622105816962755	22.17932831762693	36.67356497113604	21.525000894274275
16	24.427433360489797	21.962451734309305	32.08197902695725	21.528135878243653
17	24.766413550204557	22.641416916293114	31.54525369455851	21.046915838943818
18	24.39881658374366	22.015384732869222	32.34921631429581	21.23658236909131
19	24.411919209051582	23.624555927511132	30.766893443904486	21.1966314195328
20	24.44969978509283	25.62431397105606	30.80491517255876	19.12107107129235
21	24.537037223111586	24.470760446630717	32.29572062630549	18.696481703952212
22	23.13276536534018	21.33010939084448	33.53745562289519	21.99966962092015
23	23.35080751962078	22.425424559223263	32.16714609145874	22.05662182969722
24	22.733939135491998	23.30611390200603	31.257719395623322	22.70222756687865
25	23.249362653739812	24.833695129239715	29.4056673275588	22.51127488946167
26	21.141970160579508	24.301310543875505	30.393267662117857	24.163451633427126
27	21.18252399167059	24.257139423588985	32.033869080657915	22.52646750408251
28	19.75879915644816	24.47602561201519	31.962085986179545	23.80308924535711
29	21.86309685774126	24.985701659652477	30.70483683815163	22.446364644454636
30	23.051054821625442	24.56014768186022	31.25458441165394	21.1342130848604
31	23.065724938918052	24.93542133983588	30.565048706799015	21.433805014447053
32	24.8291936137965	25.668002786116524	28.538241378492646	20.96456222159433
33	25.352012478843882	24.398696007437145	28.45138624569995	21.797905268019026
34	23.23726483098618	25.0057173265339	30.15404426999286	21.60297357248706
35	23.64589793376422	26.94209242495431	27.868480187906115	21.543529453375353
36	25.13179995104602	27.00414903070717	27.59641984830697	20.267631169939847
37	23.7221825436858	27.28155491989513	28.643303533569046	20.35295900285002
38	24.76858392372182	25.463826907085103	28.272692159445285	21.494897009747792
39	24.957406419723714	24.62332966647388	27.578936283862348	22.84032762994006
40	25.974748909889712	23.009697148490925	28.86379740608211	22.151756535537253
41	24.664205034382334	24.1794480900914	27.753691544104203	23.402655331422064
42	23.46209945053377	24.323697544785055	29.407355395850004	22.80684760883117
43	22.543348186994557	23.894285125184332	30.061120129772263	23.501246558048848
44	23.336057018123824	23.792759875099023	28.921593649004784	23.94958945777237
45	22.511315081563843	23.509727091607044	28.358904218603236	25.62005360822588
46	23.060098044614037	24.653755087817732	29.028303680270216	23.25784318729801
47	21.615473316262406	24.898967103166296	31.16527756062878	22.320282019942518
48	22.017715874795172	25.54175939319572	29.843359320206858	22.59716541180225
49	23.8002757982051	23.540755394483472	30.33523026658216	22.32373854072927
50	23.129067691940396	23.293091660902448	29.247953518637683	24.329887128519474
51	22.356816640816447	23.821738380764703	28.39001290568401	25.431432072734843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	507.0
1	1520.5
2	2534.0
3	24453.0
4	46372.0
5	29647.0
6	12922.0
7	12678.5
8	12435.0
9	12517.5
10	12600.0
11	12526.0
12	12452.0
13	12134.5
14	11817.0
15	11157.0
16	10497.0
17	10091.5
18	9686.0
19	9549.0
20	9412.0
21	10266.5
22	11121.0
23	11384.5
24	11648.0
25	13281.0
26	17714.0
27	20514.0
28	23954.0
29	27394.0
30	31634.0
31	35874.0
32	40999.5
33	46125.0
34	50889.0
35	55653.0
36	60828.5
37	66004.0
38	72970.5
39	79937.0
40	87443.0
41	94949.0
42	105580.0
43	116211.0
44	128445.5
45	140680.0
46	178580.0
47	216480.0
48	223723.0
49	230966.0
50	226136.5
51	221307.0
52	198781.5
53	176256.0
54	161861.5
55	147467.0
56	140732.0
57	133997.0
58	126829.0
59	119661.0
60	111193.0
61	102725.0
62	93861.5
63	84998.0
64	75270.5
65	65543.0
66	54958.0
67	44373.0
68	37605.5
69	30838.0
70	25633.0
71	20428.0
72	16640.5
73	12853.0
74	10410.0
75	6443.5
76	4920.0
77	3813.0
78	2706.0
79	2001.0
80	1296.0
81	909.0
82	522.0
83	409.5
84	297.0
85	202.0
86	107.0
87	68.5
88	30.0
89	36.0
90	42.0
91	27.0
92	12.0
93	7.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2488051.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.10545757879014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.73564384492595	21.7521511155206
2	9.789630391833908	5.69863344163113
3	3.548244721358509	3.0981985864999833
4	1.76586159678441	2.0558483918089303
5	1.0581215985443149	1.539855664981658
6	0.7076547406103695	1.2357969019958908
7	0.5281701392745544	1.0760843508127456
8	0.442687554383437	1.0307699067812381
9	0.36066261565161806	0.9447525414093294
>10	6.592949875017746	46.73854227661906
>50	0.4080765138506558	7.1914099771734
>100	0.05576216227006515	2.878707859901345
>500	0.002502476997866029	0.4978727342349184
>1k	0.0033366359971547057	1.842946495507913
>5k	5.561059995257843E-4	1.173424205142913
>10k+	1.3902649988144607E-4	1.2450055499788661
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30768	1.2366305996139146	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC	7951	0.3195674043659073	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG	7935	0.31892433073116266	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC	7848	0.31542761784223877	No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT	5265	0.21161141793315327	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	3871	0.1555836275060278	No Hit
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	3733	0.15003711740635542	No Hit
TCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	3051	0.12262610372536575	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCC	2852	0.11462787539322948	No Hit
CGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG	2655	0.10671003126543628	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0096051085769545E-4	0.0	0.0	0.2292959428886305	0.0
2	2.0096051085769545E-4	0.0	0.0	0.7072202298104018	0.0
3	2.0096051085769545E-4	0.0	0.0	1.0728879753670644	0.0
4	2.0096051085769545E-4	0.0	0.0	1.5531032121126134	0.0
5	2.0096051085769545E-4	0.0	0.0	2.6313367370684926	0.0
6	2.0096051085769545E-4	0.0	0.0	3.4421722062771223	0.0
7	2.0096051085769545E-4	0.0	0.0	4.168765029334206	0.0
8	2.0096051085769545E-4	0.0	0.0	5.093746068710006	0.0
9	2.0096051085769545E-4	0.0	0.0	5.642287879147172	0.0
10	2.0096051085769545E-4	0.0	0.0	6.795318906244285	0.0
11	2.0096051085769545E-4	0.0	0.0	8.069609505592933	0.0
12	2.0096051085769545E-4	0.0	0.0	9.415442046806918	0.0
13	2.0096051085769545E-4	0.0	0.0	9.796583751699623	0.0
14	2.0096051085769545E-4	0.0	0.0	10.010244966843524	0.0
15	2.0096051085769545E-4	0.0	0.0	10.271453438856359	0.0
16	2.0096051085769545E-4	0.0	0.0	10.702996039872174	0.0
17	2.0096051085769545E-4	0.0	0.0	11.252301500250597	0.0
18	2.0096051085769545E-4	0.0	0.0	11.849194409600125	0.0
19	2.0096051085769545E-4	0.0	0.0	12.394159122944023	0.0
20	2.0096051085769545E-4	0.0	0.0	12.911029556870016	0.0
21	2.0096051085769545E-4	0.0	0.0	13.599399690761967	0.0
22	2.0096051085769545E-4	0.0	0.0	14.353644680113069	0.0
23	2.0096051085769545E-4	0.0	0.0	15.037754451174836	0.0
24	2.0096051085769545E-4	0.0	0.0	15.599398886919923	0.0
25	2.0096051085769545E-4	0.0	0.0	16.09396270414071	0.0
26	2.0096051085769545E-4	0.0	0.0	16.5467267351031	0.0
27	2.0096051085769545E-4	0.0	0.0	17.046636101912704	0.0
28	2.0096051085769545E-4	0.0	0.0	17.5137889054525	0.0
29	2.0096051085769545E-4	0.0	0.0	18.048142903823113	0.0
30	2.0096051085769545E-4	7.234578390877036E-4	0.0	18.621925354424004	0.0
31	2.0096051085769545E-4	7.234578390877036E-4	0.0	19.118016471527312	0.0
32	2.0096051085769545E-4	7.234578390877036E-4	0.0	19.6021303421835	0.0
33	2.0096051085769545E-4	7.234578390877036E-4	0.0	20.08600306022666	0.0
34	2.0096051085769545E-4	7.234578390877036E-4	0.0	20.60858077266101	0.0
35	2.0096051085769545E-4	7.234578390877036E-4	0.0	21.15643931736126	0.0
36	2.0096051085769545E-4	7.234578390877036E-4	0.0	21.66273922841614	0.0
37	2.0096051085769545E-4	7.234578390877036E-4	0.0	22.15665997200218	0.0
38	2.0096051085769545E-4	7.234578390877036E-4	0.0	22.723609765233913	0.0
39	2.0096051085769545E-4	7.234578390877036E-4	0.0	23.607755628803428	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATCG	40	6.8212103E-9	45.0	1
CGTTTTT	14300	0.0	43.222027	1
TCGTTTG	910	0.0	40.54945	1
TTTCGCG	780	0.0	38.653847	1
TACGCGG	100	0.0	38.25	2
TTTGGGC	6725	0.0	38.141262	4
CGTTTGG	1650	0.0	37.636364	2
GCCGATA	30	1.1403043E-4	37.499996	9
TTTGGGA	10000	0.0	37.125	4
TTGGGAC	4080	0.0	37.11397	5
GTTTTTT	17260	0.0	36.982906	2
TGGGCGA	2690	0.0	36.97026	6
GGGCGAT	3740	0.0	36.9385	7
TTGGGCG	2545	0.0	36.866405	5
ATACGGA	220	0.0	36.81818	4
TTTGTCG	710	0.0	36.44366	1
TTGGGCA	4115	0.0	36.3062	5
ACGCTTA	25	0.0021075793	36.0	17
TACGGGA	245	0.0	35.816326	4
TTGGGAT	5245	0.0	35.64824	5
>>END_MODULE