SRR2934381.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934381.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	630649
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	3515	0.557362336259948	RNA PCR Primer, Index 40 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	3139	0.49774121579515707	Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	3128	0.4959969808879424	Illumina PCR Primer Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2151	0.3410772077653338	RNA PCR Primer, Index 40 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1815	0.2877987596904142	No Hit
TCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	1087	0.17236212219475494	RNA PCR Primer, Index 40 (95% over 24bp)
TTCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	850	0.13478178828476697	Illumina PCR Primer Index 8 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	833	0.13208615251907163	TruSeq Adapter, Index 19 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC	761	0.12066934221730313	No Hit
GCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	720	0.11416810301768496	Illumina PCR Primer Index 8 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGATG	25	3.888795E-5	45.000004	20
CACGAAG	30	2.163937E-6	45.000004	34
TATTGCG	25	3.888795E-5	45.000004	1
TGATTCG	30	2.163937E-6	45.000004	15
GCGATAG	30	2.163937E-6	45.000004	9
CGTCATA	25	3.888795E-5	45.000004	38
CTCACGG	20	7.030862E-4	45.0	2
ACGCCTA	20	7.030862E-4	45.0	9
TAGCGAA	20	7.030862E-4	45.0	1
TGCGGTA	35	1.2107557E-7	45.0	4
ACTTACG	35	1.2107557E-7	45.0	37
TACGCGG	20	7.030862E-4	45.0	2
CGTAGCG	20	7.030862E-4	45.0	2
CGAGGAC	20	7.030862E-4	45.0	37
ACGCTGG	20	7.030862E-4	45.0	2
GTTTTCG	110	0.0	45.0	1
CGTCATC	20	7.030862E-4	45.0	11
TCGGTTG	40	6.8066583E-9	45.0	1
TAGTCCG	40	6.8066583E-9	45.0	1
GTCACGG	80	0.0	42.1875	1