FastQCFastQC Report
Sat 14 Jan 2017
SRR2934380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948368
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389762.0004434480549875No Hit
CTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGCT62550.3210379148087014TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCT61570.316008064185No Hit
TCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC58740.30148308738390284TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTT54790.28120970987000404No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCC49420.2536481814523745No Hit
GCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC44340.22757507821930972TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTC41530.21315275143094117No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGT39680.20365762525354553No Hit
CCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC38010.1950863491907073TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTG34960.17943222224959557No Hit
CGTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCT33470.17178479630131474No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTC32820.16844867088763518No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCG27700.14217026762911317No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGC23200.11907401476517782No Hit
TCCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTG23080.11845811468880621No Hit
TTCCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCT22980.11794486462516321No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG253.891807E-545.0000041
CCGCGCA253.891807E-545.00000442
GCCCGCG253.891807E-545.0000041
CGGCGCC351.212502E-745.042
CCGCTCG207.0344965E-445.01
GTCCGCG406.8193913E-945.01
CAGTACG207.0344965E-445.022
AGTACGT207.0344965E-445.023
CCGTGCG302.1662818E-644.9999961
CGTTTTT301100.044.589011
TATCGCG1050.042.8571431
CTTCGCG900.042.51
ACGGGGC1250.041.3999985
TTTCGCG5600.040.982141
TTCGTAG1000.040.5000041
TTCCGCG1900.040.263161
GGCGCGA451.929402E-840.08
TTCTCCG2950.039.6610151
GTTTTTT339450.039.4520532
GCGCGAC356.250526E-638.5714269
Produced by FastQC (version 0.11.5)